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The map label for this gene is mleN [H]

Identifier: 188587062

GI number: 188587062

Start: 2635882

End: 2637198

Strand: Reverse

Name: mleN [H]

Synonym: Nther_2454

Alternate gene names: 188587062

Gene position: 2637198-2635882 (Counterclockwise)

Preceding gene: 188587063

Following gene: 188587060

Centisome position: 83.31

GC content: 40.39

Gene sequence:

>1317_bases
ATGGATCAAAAAGAAATTAAACCGAATGGTTGGGCATTAATACCCTTATTTGTGTTTTTATTTTTGTATTTAGGTGTAGG
ACTGTTTCTGACTTATTTAGGAGTTGAAATGGCCTTTTACGAATTTCCTGCACCTATTGCAGCATTGATTGGTATTGTAG
TAGCAGTATTTATGGCAAGAGGTACAATTGATGAACGGATCAAGACTTTTATTAACGGGGCCGGTGAAGAAGGTATTGTG
ATTATGTGTATGATTTATTTGCTGGCCGGTGCCTTTTCAGCAGTAGTAGAAGGTATGGGAGGCGTTGAATCTACTGTTAA
TCTAGGGCTTACTATGGTGCCTGCTCGCCTGTTGCTACCTGGTTTATTCTTGATAAGTGCTTTTATATCCCTGGCCATGG
GAACTTCAATGGGAACTATCGGTGCAGTTGCGCCAATTGCCGTTGGCTTGGGTCCCGAAATTGGAGTTTCTATGCCGGTC
ATGATGGGAATTGTGATTGGTGGTGCCATGTTTGGTGACAATTTGTCAATTATATCCGATACAACAATTGCCGCTACTCA
GACCCAGGGAGTTAAAATGAAAGATAAGTTTAAAATGAACTTTTTGATTGTACTCCCTGCCGCTATTATATCTTTGATTA
TTTTACTATTTGTCGGTGATGTTGCTGCTTTAGATGGAGATTATCCCTTTGAAATTATTAAGGTTTTACCCTATATATTT
GTACTTGTCTTTGCTTTGATGGGTGTAAATGTTTTTGTTGTTCTGTTTTTAGGTTTGGTATTGGCTGGGCTAATTGGAGT
TGTTTACGGAGATTTTACAGCTTTAGAACTGTCTCAAGCCGCATATCAAGGTTTTACTGATATGAACGAAGTTTTCTTCT
TGTCATTGTTTGCTGGTGGACTGGCTGCCATGATACGAAAAGAAGGTGGACTGGCCTTTTTGGTCGATCAAATATCAAGG
CGGGTCAAAACTCGAAAAGGGGCAGAGCTGGGGATTGGTGCTTTATCAGCAGCTGCCGACATTTGTACTGCCAATAACAC
TGTTGCAATTATAATCAGTGGACCTTTGGCGAAGAATATTGCTTTAGAAAATGGTGTTGATCTGCGAAGAAGTGCTAGTA
TGCTGGATATTTTTGCATCAGTAACCCAGGGAATGCTTCCCTATGGGGCTCAAATCTTGCTGGCAGGTTCACTTGCTGGA
ATTTCGCCACTCACAATAATCCCGAACTTGCACTACAATTTCTTAATGCTCGGTTGTGTGCTTTTGGCAATATATTTAGG
TATACCTAATTTGAAACCTTCAGAAGATAGCGCTTAG

Upstream 100 bases:

>100_bases
CCTCAGCGCCTCCCTACATTACATTGTTAACCCATCTGAAGAAATTGGTAAGAAAATTACAAAACTACTGACAATGAAGA
TTTAAAGGGGGATCACCTAG

Downstream 100 bases:

>100_bases
TGAACACCTACAGACTAAGTTAACGAATACAGCATTTAGCTAGCGAATTACATTTAATTAGCGTATACAGTTGATTAACT
TGGTATAACTTGTATAAATC

Product: Na+/H+ antiporter NhaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MDQKEIKPNGWALIPLFVFLFLYLGVGLFLTYLGVEMAFYEFPAPIAALIGIVVAVFMARGTIDERIKTFINGAGEEGIV
IMCMIYLLAGAFSAVVEGMGGVESTVNLGLTMVPARLLLPGLFLISAFISLAMGTSMGTIGAVAPIAVGLGPEIGVSMPV
MMGIVIGGAMFGDNLSIISDTTIAATQTQGVKMKDKFKMNFLIVLPAAIISLIILLFVGDVAALDGDYPFEIIKVLPYIF
VLVFALMGVNVFVVLFLGLVLAGLIGVVYGDFTALELSQAAYQGFTDMNEVFFLSLFAGGLAAMIRKEGGLAFLVDQISR
RVKTRKGAELGIGALSAAADICTANNTVAIIISGPLAKNIALENGVDLRRSASMLDIFASVTQGMLPYGAQILLAGSLAG
ISPLTIIPNLHYNFLMLGCVLLAIYLGIPNLKPSEDSA

Sequences:

>Translated_438_residues
MDQKEIKPNGWALIPLFVFLFLYLGVGLFLTYLGVEMAFYEFPAPIAALIGIVVAVFMARGTIDERIKTFINGAGEEGIV
IMCMIYLLAGAFSAVVEGMGGVESTVNLGLTMVPARLLLPGLFLISAFISLAMGTSMGTIGAVAPIAVGLGPEIGVSMPV
MMGIVIGGAMFGDNLSIISDTTIAATQTQGVKMKDKFKMNFLIVLPAAIISLIILLFVGDVAALDGDYPFEIIKVLPYIF
VLVFALMGVNVFVVLFLGLVLAGLIGVVYGDFTALELSQAAYQGFTDMNEVFFLSLFAGGLAAMIRKEGGLAFLVDQISR
RVKTRKGAELGIGALSAAADICTANNTVAIIISGPLAKNIALENGVDLRRSASMLDIFASVTQGMLPYGAQILLAGSLAG
ISPLTIIPNLHYNFLMLGCVLLAIYLGIPNLKPSEDSA
>Mature_438_residues
MDQKEIKPNGWALIPLFVFLFLYLGVGLFLTYLGVEMAFYEFPAPIAALIGIVVAVFMARGTIDERIKTFINGAGEEGIV
IMCMIYLLAGAFSAVVEGMGGVESTVNLGLTMVPARLLLPGLFLISAFISLAMGTSMGTIGAVAPIAVGLGPEIGVSMPV
MMGIVIGGAMFGDNLSIISDTTIAATQTQGVKMKDKFKMNFLIVLPAAIISLIILLFVGDVAALDGDYPFEIIKVLPYIF
VLVFALMGVNVFVVLFLGLVLAGLIGVVYGDFTALELSQAAYQGFTDMNEVFFLSLFAGGLAAMIRKEGGLAFLVDQISR
RVKTRKGAELGIGALSAAADICTANNTVAIIISGPLAKNIALENGVDLRRSASMLDIFASVTQGMLPYGAQILLAGSLAG
ISPLTIIPNLHYNFLMLGCVLLAIYLGIPNLKPSEDSA

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 46133; Mature: 46133

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQKEIKPNGWALIPLFVFLFLYLGVGLFLTYLGVEMAFYEFPAPIAALIGIVVAVFMAR
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GTIDERIKTFINGAGEEGIVIMCMIYLLAGAFSAVVEGMGGVESTVNLGLTMVPARLLLP
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHH
GLFLISAFISLAMGTSMGTIGAVAPIAVGLGPEIGVSMPVMMGIVIGGAMFGDNLSIISD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEC
TTIAATQTQGVKMKDKFKMNFLIVLPAAIISLIILLFVGDVAALDGDYPFEIIKVLPYIF
CEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VLVFALMGVNVFVVLFLGLVLAGLIGVVYGDFTALELSQAAYQGFTDMNEVFFLSLFAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LAAMIRKEGGLAFLVDQISRRVKTRKGAELGIGALSAAADICTANNTVAIIISGPLAKNI
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHH
ALENGVDLRRSASMLDIFASVTQGMLPYGAQILLAGSLAGISPLTIIPNLHYNFLMLGCV
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEECCCHHHHHHHHHH
LLAIYLGIPNLKPSEDSA
HHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDQKEIKPNGWALIPLFVFLFLYLGVGLFLTYLGVEMAFYEFPAPIAALIGIVVAVFMAR
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GTIDERIKTFINGAGEEGIVIMCMIYLLAGAFSAVVEGMGGVESTVNLGLTMVPARLLLP
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHH
GLFLISAFISLAMGTSMGTIGAVAPIAVGLGPEIGVSMPVMMGIVIGGAMFGDNLSIISD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEC
TTIAATQTQGVKMKDKFKMNFLIVLPAAIISLIILLFVGDVAALDGDYPFEIIKVLPYIF
CEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VLVFALMGVNVFVVLFLGLVLAGLIGVVYGDFTALELSQAAYQGFTDMNEVFFLSLFAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LAAMIRKEGGLAFLVDQISRRVKTRKGAELGIGALSAAADICTANNTVAIIISGPLAKNI
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHH
ALENGVDLRRSASMLDIFASVTQGMLPYGAQILLAGSLAGISPLTIIPNLHYNFLMLGCV
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEECCCHHHHHHHHHH
LLAIYLGIPNLKPSEDSA
HHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]