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The map label for this gene is rpfG [H]

Identifier: 188587058

GI number: 188587058

Start: 2629490

End: 2631343

Strand: Reverse

Name: rpfG [H]

Synonym: Nther_2450

Alternate gene names: 188587058

Gene position: 2631343-2629490 (Counterclockwise)

Preceding gene: 188587059

Following gene: 188587057

Centisome position: 83.12

GC content: 39.97

Gene sequence:

>1854_bases
ATGAACGACTTAAAAAGCGATGTTAATAGAATTACTGACCACATAAATAAAGAATTGAAAGTAAAAGAACAGGCTATAAA
TTCGTCCATTAATGCCATTGCCTTTACGGATTTGGAAGGATATATCACTTATGTCAATCAATCCTTTTTAGATTTGTGGG
GGTATGAAGAAGAACAAGAAATTTTAGGCCGGTCCGCTCTGGAGTTTTGGAAAAACATTGACAAAACCCAAAAGATTTTG
GATGAGCTTTTTGAAAAGGGGACTTGGGTTGGCGAATTGATTGCCCGAAAAAAAGATGGAGAATTGTTTGATGTTCACTT
GTCTGCCTCTTTAGTTGAGGATGAGCAGGGCAAGCCTTTAAAATTAGTGGGTTCTTTTATAGATATTAGTAAGCAAAAAC
GGGCAGAGGACAAGCTTCTTCGGGAAAAGGCTAGGTATCAGGCTTTAATAGAAGAGCAGTTAGAGCTGGTGTGCAGGTGG
CTTCCTGATACCACTTTAACCTTTGTTAACAAATCTTATTGTAATTTTTTTGGTCAGACAAAACATGATTTGCTGGGAAA
AAAGTGGATAGAGTTTGTCCCTGAGCAGGAAAGAGATAAAGTTTGGCAAAAATATCAAAACCTTACGAATAACCCCAGGG
TTCTTTCATACGAACATCAGGTTACCGGTGCCGATGGGAAAGCTTATTGGCAGCACTGGATTGATTGCCCTATTTTTAAT
GACATGGGTGAGCTAGTTGAATTTCAGTCAGTAGCCCGAGATGTTACTGTACGCAGAAATAAGGATGAAAAAATTCAGTA
CTTAAGTTTTCACGATAGTTTAACTGGACTGTATAATCGTGCTTATTTAGAAGGTGAACTAACTCGCTTGGATGGTGAAA
CCCGAAAAGGAAGTAGCAGTTATTTCCCTATTAGTGTGATAATGGCAGATTTAAATGGACTTAAAATGATTAATGATTCT
TACGGCCATCATGTTGGTGATGAAATGTTACTAAAGGTAGCTCAGATTTTGCATGAATCATGTGATGAATCATCTCGTAA
AAAAGCTGAAATTGCCAGGTGGGGAGGAGATGAGTTTGTAATCCTACTCCCGGATATTACGGATGAAGAAGCTAGGGACA
TATGTAAGGTAATAAATTATAAATGCAAGGGTACTTATGTGAAAAACATCCCCCTCTCTCTGGCAGTGGGTACGGCAACT
AAAGAGAGTATTCAGGAGGACATGATGGAAGTTCTTACTAGGGCAGAAAATGATATGTACAAGTTAAAACTGGCCGAGAG
CCGAAGTGCCCGAAGCGATGTTGTGAATGCCCTCTTAAAAGCCTTAGAAGCCAAGAGCTTTGAAACAGAAAGCCATGCCC
GGCGCATGTTGATGATGGCACGAGAAATTGGCAAACAAATTGGTTTATCTCATTCAGAACTGAACCGGCTAAACCTTTTG
ATAACCCTCCACGATATCGGTAAAATCAATATCCCTGAAGAAGTGCTTACTAAAAATGGGACATTAAATGAAGACGAGTG
GACAATCATGAAAAAACATCCCGAGATAGGTTACAGAATTGCTATAGCTACTGAAGAATTTGCTCATGTTGCCGAAGAAA
TCCTGTCCCATCATGAACACTGGGATGGTTCAGGTTATCCCAGGGGATTATCAGGGGAGGAGATCCCCCTTCTGGCCAGA
ATTGCTGCCATTGCCGATGCTTATGAAGTGATGAGTAATGGTAGGCCTTACAAAGAGCCAATGGGTGAAAAAGAAATTGT
CGCTGAATTTAAGCGATGTGCGGGAACTCAGTTTGATCCAAAATTGGTTGAAATATTTCTAGAGCTATTGAGGGGGGGTA
ACATTAATGAATAA

Upstream 100 bases:

>100_bases
TAGAAAATAAATGTTATAATAAATTTATGGGAATTTTCGACAAATTTTAACAAAAAATTATTGATTTAGTAAGATTTATA
CGTGGGGGGGGGCTTAGTAT

Downstream 100 bases:

>100_bases
TTTAGACAAGAAAGCTAATTTATTCACAGTGGTTTTGGTAGTTGTTTTTTCAACATTTTACATCATGGGACATGTGGCTT
CATACTTTGAGGGTGATCGG

Product: diguanylate cyclase and metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MNDLKSDVNRITDHINKELKVKEQAINSSINAIAFTDLEGYITYVNQSFLDLWGYEEEQEILGRSALEFWKNIDKTQKIL
DELFEKGTWVGELIARKKDGELFDVHLSASLVEDEQGKPLKLVGSFIDISKQKRAEDKLLREKARYQALIEEQLELVCRW
LPDTTLTFVNKSYCNFFGQTKHDLLGKKWIEFVPEQERDKVWQKYQNLTNNPRVLSYEHQVTGADGKAYWQHWIDCPIFN
DMGELVEFQSVARDVTVRRNKDEKIQYLSFHDSLTGLYNRAYLEGELTRLDGETRKGSSSYFPISVIMADLNGLKMINDS
YGHHVGDEMLLKVAQILHESCDESSRKKAEIARWGGDEFVILLPDITDEEARDICKVINYKCKGTYVKNIPLSLAVGTAT
KESIQEDMMEVLTRAENDMYKLKLAESRSARSDVVNALLKALEAKSFETESHARRMLMMAREIGKQIGLSHSELNRLNLL
ITLHDIGKINIPEEVLTKNGTLNEDEWTIMKKHPEIGYRIAIATEEFAHVAEEILSHHEHWDGSGYPRGLSGEEIPLLAR
IAAIADAYEVMSNGRPYKEPMGEKEIVAEFKRCAGTQFDPKLVEIFLELLRGGNINE

Sequences:

>Translated_617_residues
MNDLKSDVNRITDHINKELKVKEQAINSSINAIAFTDLEGYITYVNQSFLDLWGYEEEQEILGRSALEFWKNIDKTQKIL
DELFEKGTWVGELIARKKDGELFDVHLSASLVEDEQGKPLKLVGSFIDISKQKRAEDKLLREKARYQALIEEQLELVCRW
LPDTTLTFVNKSYCNFFGQTKHDLLGKKWIEFVPEQERDKVWQKYQNLTNNPRVLSYEHQVTGADGKAYWQHWIDCPIFN
DMGELVEFQSVARDVTVRRNKDEKIQYLSFHDSLTGLYNRAYLEGELTRLDGETRKGSSSYFPISVIMADLNGLKMINDS
YGHHVGDEMLLKVAQILHESCDESSRKKAEIARWGGDEFVILLPDITDEEARDICKVINYKCKGTYVKNIPLSLAVGTAT
KESIQEDMMEVLTRAENDMYKLKLAESRSARSDVVNALLKALEAKSFETESHARRMLMMAREIGKQIGLSHSELNRLNLL
ITLHDIGKINIPEEVLTKNGTLNEDEWTIMKKHPEIGYRIAIATEEFAHVAEEILSHHEHWDGSGYPRGLSGEEIPLLAR
IAAIADAYEVMSNGRPYKEPMGEKEIVAEFKRCAGTQFDPKLVEIFLELLRGGNINE
>Mature_617_residues
MNDLKSDVNRITDHINKELKVKEQAINSSINAIAFTDLEGYITYVNQSFLDLWGYEEEQEILGRSALEFWKNIDKTQKIL
DELFEKGTWVGELIARKKDGELFDVHLSASLVEDEQGKPLKLVGSFIDISKQKRAEDKLLREKARYQALIEEQLELVCRW
LPDTTLTFVNKSYCNFFGQTKHDLLGKKWIEFVPEQERDKVWQKYQNLTNNPRVLSYEHQVTGADGKAYWQHWIDCPIFN
DMGELVEFQSVARDVTVRRNKDEKIQYLSFHDSLTGLYNRAYLEGELTRLDGETRKGSSSYFPISVIMADLNGLKMINDS
YGHHVGDEMLLKVAQILHESCDESSRKKAEIARWGGDEFVILLPDITDEEARDICKVINYKCKGTYVKNIPLSLAVGTAT
KESIQEDMMEVLTRAENDMYKLKLAESRSARSDVVNALLKALEAKSFETESHARRMLMMAREIGKQIGLSHSELNRLNLL
ITLHDIGKINIPEEVLTKNGTLNEDEWTIMKKHPEIGYRIAIATEEFAHVAEEILSHHEHWDGSGYPRGLSGEEIPLLAR
IAAIADAYEVMSNGRPYKEPMGEKEIVAEFKRCAGTQFDPKLVEIFLELLRGGNINE

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=268, Percent_Identity=25.3731343283582, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788381, Length=302, Percent_Identity=26.158940397351, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI145693134, Length=134, Percent_Identity=34.3283582089552, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI87082007, Length=189, Percent_Identity=29.1005291005291, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=29.6511627906977, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=147, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786584, Length=197, Percent_Identity=26.9035532994924, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081974, Length=101, Percent_Identity=42.5742574257426, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=27.1676300578035, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 70719; Mature: 70719

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLKSDVNRITDHINKELKVKEQAINSSINAIAFTDLEGYITYVNQSFLDLWGYEEEQE
CCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCHHHH
ILGRSALEFWKNIDKTQKILDELFEKGTWVGELIARKKDGELFDVHLSASLVEDEQGKPL
HHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCCH
KLVGSFIDISKQKRAEDKLLREKARYQALIEEQLELVCRWLPDTTLTFVNKSYCNFFGQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
KHDLLGKKWIEFVPEQERDKVWQKYQNLTNNPRVLSYEHQVTGADGKAYWQHWIDCPIFN
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECEECCCCCHHHHHHHCCCCCHH
DMGELVEFQSVARDVTVRRNKDEKIQYLSFHDSLTGLYNRAYLEGELTRLDGETRKGSSS
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCC
YFPISVIMADLNGLKMINDSYGHHVGDEMLLKVAQILHESCDESSRKKAEIARWGGDEFV
CCHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEE
ILLPDITDEEARDICKVINYKCKGTYVKNIPLSLAVGTATKESIQEDMMEVLTRAENDMY
EECCCCCCHHHHHHHHHHCCCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCEE
KLKLAESRSARSDVVNALLKALEAKSFETESHARRMLMMAREIGKQIGLSHSELNRLNLL
EEEEHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEE
ITLHDIGKINIPEEVLTKNGTLNEDEWTIMKKHPEIGYRIAIATEEFAHVAEEILSHHEH
EEEECCCCCCCCHHHHHCCCCCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHCCC
WDGSGYPRGLSGEEIPLLARIAAIADAYEVMSNGRPYKEPMGEKEIVAEFKRCAGTQFDP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCH
KLVEIFLELLRGGNINE
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNDLKSDVNRITDHINKELKVKEQAINSSINAIAFTDLEGYITYVNQSFLDLWGYEEEQE
CCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCHHHH
ILGRSALEFWKNIDKTQKILDELFEKGTWVGELIARKKDGELFDVHLSASLVEDEQGKPL
HHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCCH
KLVGSFIDISKQKRAEDKLLREKARYQALIEEQLELVCRWLPDTTLTFVNKSYCNFFGQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
KHDLLGKKWIEFVPEQERDKVWQKYQNLTNNPRVLSYEHQVTGADGKAYWQHWIDCPIFN
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECEECCCCCHHHHHHHCCCCCHH
DMGELVEFQSVARDVTVRRNKDEKIQYLSFHDSLTGLYNRAYLEGELTRLDGETRKGSSS
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCC
YFPISVIMADLNGLKMINDSYGHHVGDEMLLKVAQILHESCDESSRKKAEIARWGGDEFV
CCHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEE
ILLPDITDEEARDICKVINYKCKGTYVKNIPLSLAVGTATKESIQEDMMEVLTRAENDMY
EECCCCCCHHHHHHHHHHCCCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCEE
KLKLAESRSARSDVVNALLKALEAKSFETESHARRMLMMAREIGKQIGLSHSELNRLNLL
EEEEHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEE
ITLHDIGKINIPEEVLTKNGTLNEDEWTIMKKHPEIGYRIAIATEEFAHVAEEILSHHEH
EEEECCCCCCCCHHHHHCCCCCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHCCC
WDGSGYPRGLSGEEIPLLARIAAIADAYEVMSNGRPYKEPMGEKEIVAEFKRCAGTQFDP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCH
KLVEIFLELLRGGNINE
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]