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The map label for this gene is ytsJ [H]

Identifier: 188587007

GI number: 188587007

Start: 2562894

End: 2564093

Strand: Reverse

Name: ytsJ [H]

Synonym: Nther_2399

Alternate gene names: 188587007

Gene position: 2564093-2562894 (Counterclockwise)

Preceding gene: 188587008

Following gene: 188587006

Centisome position: 81.0

GC content: 43.42

Gene sequence:

>1200_bases
TTGAATAACGAGGAAATGAAGAAACTTGCACTTGAACTACATCAACGATCTCCAGGGAAGCTTGAGGTCAAAAGTAAAGT
CACTATTAAGAACAAGGAAGACCTCTCCCTGGCATATAGTCCAGGTGTTGCTGAACCCTGTCGTGAGATCGCTGCAAACT
CTGAGAAAGTATGGCAATATACAAATCGAGGGAATAATGTGGCAGTTGTGACAGACGGCTCTGCTGTCCTGGGTTTAGGC
AATATCGGTGCCCAAGCTGCCATGCCTGTTATGGAAGGAAAAGCTGTTTTATTCAAGGAATTTGCCAATATAGATGCTTT
TCCCTTGTGTGTAAACACGCAAAATGTGGACGAAATTGTCAGGATAGTTAAGGGAACTGAGGCTACCTGGGGTGGTATTA
ACCTAGAGGATATAAGCTCTCCCAGGTGTATTGAAATTGAGCGTCGGTTAAAAGAAGAAACAGATATACCCATATTCCAC
GATGACCAACACGGAACAGCCATTGTAGCCACTGCTGCCTTAATCAATTCTTTAAAACTTGTTAATAAGAAAATTGAAGA
TGTACGAATTGTAGTCAATGGTTCGGGGGCAGGAGGGATTGCCACGGCCAAGCTTCTCATGGATATGAACTGTGGAGATT
TAATTTTGTGTGATTCCAGGGGGATTATTCATAAGGGACGCAGACAAGGTATGAATTGGATTAAGGAAGAAATGGCGGAG
AGGACTAATCAAGACAATATTACCGGAGATTTAGCCCGAGCCATGGAAGGTGCCGATGTCTTCTTAGGATTGTCTGTTCC
TGACAGTGTGACTAAAGAGATGGTAGTGAGCATGAATAGGGATCCGATTATTTTTGCCCTGGCAAATCCAGTTCCGGAGA
TTTATCCTCATCTAGCTCAGGAGGCAGGTGCTAAAGTAGTAGGAACGGGGCGGAGTGATTTCCCAAATCAAATCAATAAT
GTTTTGGCTTTTCCCGGGATACTGCGGGGAGCCCTGGATGTGCAAGCAAAAGATATCAACGAGGATATGAAAATTGCAGC
TTCGCAAGCTATTGCAGAGTTAATCCCTCCTGCCAATTTAAGACAAGATTATGTGATACCTGATGCCATAGACAAAAAAA
TTGCCAAAACTGTAGCCAGGGCTGTGGCAAAGGCTGCAATAGATTCTGGGCTAAATCGAAAGGATATCAATTCAATATAG

Upstream 100 bases:

>100_bases
AAGGAAGCTATTATCTCAACATAGAAGTATAATATAAGAAGAACGAGTCAGGGGAACGAGTCTAGGGAATAATGAATCTT
TTCAGGAGGTGTATTACGTA

Downstream 100 bases:

>100_bases
TTCATGCTATTATAAGCCATAAAGCAAACATAAGCTAAGGAGGAATCTCTTTGCGTAAAATCCATGTTTCCGAGATTACC
AGGAGTGTAAAAGAGATGTT

Product: Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MNNEEMKKLALELHQRSPGKLEVKSKVTIKNKEDLSLAYSPGVAEPCREIAANSEKVWQYTNRGNNVAVVTDGSAVLGLG
NIGAQAAMPVMEGKAVLFKEFANIDAFPLCVNTQNVDEIVRIVKGTEATWGGINLEDISSPRCIEIERRLKEETDIPIFH
DDQHGTAIVATAALINSLKLVNKKIEDVRIVVNGSGAGGIATAKLLMDMNCGDLILCDSRGIIHKGRRQGMNWIKEEMAE
RTNQDNITGDLARAMEGADVFLGLSVPDSVTKEMVVSMNRDPIIFALANPVPEIYPHLAQEAGAKVVGTGRSDFPNQINN
VLAFPGILRGALDVQAKDINEDMKIAASQAIAELIPPANLRQDYVIPDAIDKKIAKTVARAVAKAAIDSGLNRKDINSI

Sequences:

>Translated_399_residues
MNNEEMKKLALELHQRSPGKLEVKSKVTIKNKEDLSLAYSPGVAEPCREIAANSEKVWQYTNRGNNVAVVTDGSAVLGLG
NIGAQAAMPVMEGKAVLFKEFANIDAFPLCVNTQNVDEIVRIVKGTEATWGGINLEDISSPRCIEIERRLKEETDIPIFH
DDQHGTAIVATAALINSLKLVNKKIEDVRIVVNGSGAGGIATAKLLMDMNCGDLILCDSRGIIHKGRRQGMNWIKEEMAE
RTNQDNITGDLARAMEGADVFLGLSVPDSVTKEMVVSMNRDPIIFALANPVPEIYPHLAQEAGAKVVGTGRSDFPNQINN
VLAFPGILRGALDVQAKDINEDMKIAASQAIAELIPPANLRQDYVIPDAIDKKIAKTVARAVAKAAIDSGLNRKDINSI
>Mature_399_residues
MNNEEMKKLALELHQRSPGKLEVKSKVTIKNKEDLSLAYSPGVAEPCREIAANSEKVWQYTNRGNNVAVVTDGSAVLGLG
NIGAQAAMPVMEGKAVLFKEFANIDAFPLCVNTQNVDEIVRIVKGTEATWGGINLEDISSPRCIEIERRLKEETDIPIFH
DDQHGTAIVATAALINSLKLVNKKIEDVRIVVNGSGAGGIATAKLLMDMNCGDLILCDSRGIIHKGRRQGMNWIKEEMAE
RTNQDNITGDLARAMEGADVFLGLSVPDSVTKEMVVSMNRDPIIFALANPVPEIYPHLAQEAGAKVVGTGRSDFPNQINN
VLAFPGILRGALDVQAKDINEDMKIAASQAIAELIPPANLRQDYVIPDAIDKKIAKTVARAVAKAAIDSGLNRKDINSI

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=411, Percent_Identity=24.0875912408759, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI62420882, Length=357, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI62420880, Length=357, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI239049447, Length=357, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4505145, Length=362, Percent_Identity=24.8618784530387, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788806, Length=412, Percent_Identity=48.5436893203884, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17537199, Length=410, Percent_Identity=25.609756097561, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322823, Length=323, Percent_Identity=26.9349845201238, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281362674, Length=444, Percent_Identity=24.0990990990991, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281362672, Length=444, Percent_Identity=24.0990990990991, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356279, Length=444, Percent_Identity=24.0990990990991, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24646388, Length=358, Percent_Identity=25.4189944134078, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24646386, Length=358, Percent_Identity=25.4189944134078, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 43164; Mature: 43164

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNEEMKKLALELHQRSPGKLEVKSKVTIKNKEDLSLAYSPGVAEPCREIAANSEKVWQY
CCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEECCCCHHHHHHHHCCCHHHHHH
TNRGNNVAVVTDGSAVLGLGNIGAQAAMPVMEGKAVLFKEFANIDAFPLCVNTQNVDEIV
HCCCCEEEEEECCCEEEEECCCCHHHHCHHHCCCHHHHHHHCCCCCEEEEECCCCHHHHH
RIVKGTEATWGGINLEDISSPRCIEIERRLKEETDIPIFHDDQHGTAIVATAALINSLKL
HHHCCCCCCCCCCCHHHCCCCCEEHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
VNKKIEDVRIVVNGSGAGGIATAKLLMDMNCGDLILCDSRGIIHKGRRQGMNWIKEEMAE
HHHCCCEEEEEEECCCCCCHHHHHHHEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
RTNQDNITGDLARAMEGADVFLGLSVPDSVTKEMVVSMNRDPIIFALANPVPEIYPHLAQ
HCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHH
EAGAKVVGTGRSDFPNQINNVLAFPGILRGALDVQAKDINEDMKIAASQAIAELIPPANL
HCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCCC
RQDYVIPDAIDKKIAKTVARAVAKAAIDSGLNRKDINSI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNNEEMKKLALELHQRSPGKLEVKSKVTIKNKEDLSLAYSPGVAEPCREIAANSEKVWQY
CCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEECCCCHHHHHHHHCCCHHHHHH
TNRGNNVAVVTDGSAVLGLGNIGAQAAMPVMEGKAVLFKEFANIDAFPLCVNTQNVDEIV
HCCCCEEEEEECCCEEEEECCCCHHHHCHHHCCCHHHHHHHCCCCCEEEEECCCCHHHHH
RIVKGTEATWGGINLEDISSPRCIEIERRLKEETDIPIFHDDQHGTAIVATAALINSLKL
HHHCCCCCCCCCCCHHHCCCCCEEHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
VNKKIEDVRIVVNGSGAGGIATAKLLMDMNCGDLILCDSRGIIHKGRRQGMNWIKEEMAE
HHHCCCEEEEEEECCCCCCHHHHHHHEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
RTNQDNITGDLARAMEGADVFLGLSVPDSVTKEMVVSMNRDPIIFALANPVPEIYPHLAQ
HCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHH
EAGAKVVGTGRSDFPNQINNVLAFPGILRGALDVQAKDINEDMKIAASQAIAELIPPANL
HCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCCC
RQDYVIPDAIDKKIAKTVARAVAKAAIDSGLNRKDINSI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]