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The map label for this gene is appA [H]

Identifier: 188586996

GI number: 188586996

Start: 2551271

End: 2553022

Strand: Direct

Name: appA [H]

Synonym: Nther_2388

Alternate gene names: 188586996

Gene position: 2551271-2553022 (Clockwise)

Preceding gene: 188586982

Following gene: 188586997

Centisome position: 80.59

GC content: 43.72

Gene sequence:

>1752_bases
TTGAAAGGTTATTGGTGGACCTTTGTGACTGTGTTAGTCTTAAGTTTTACCTTAGTGTTTACTGGCTGTGGCCCCGATGA
TGCCGATCCTGCCGAAGAAGAACCAGAAGAAGCTCCTGACCCCGACAGAAAAGTTGAAGAAATTGTAATCAACACTACCA
CTATGGATTACGACCCGGCTAGAAATGAAGTCGCCAGGTGGGTAGGAGATACTTTTGAAACTGAACTAGGCGTTGATGTG
GAAGTTCAACCTAGAGAGTTTACAACTTTAGTAGACTCAGCTCGTCGGGATCCTGGTGAATCTGAATGGCAAGCCATCAC
TCTAGGTTGGTCTGGGCGGATAGAGAGAGCCGACCCTGACATGTTCACTCACACCTTGTTCCATTCCGATCAAGCTTACG
ACGGTGGAAACAACTATCATAATTATGAAAGTGATGAATATGACGAGCTAGCAGAACAGGCCAGGATGGCCTTCGATGAA
GATGAAAGACAGGAACTGGTCTATGAAATGCAAGAAAAATTAGCTGAAGATATTCCCATGATTGTATTGTACAACGAGGC
TGAACATCAAGCTCAAAACGTGGAACGCTGGGACAATGTAATACAAACTCCTGAAGGAAAATACAGTGAATGGTTCCCCT
ATTACGCTGAACCATTAACTGATGATGATCATTTTAGAATCGGCTATACCCAGGATTTGGATACATTTAACCCACTAGCA
GCTACCACAGTTTTTGAGTGGAAATTACTACGACTAAATTACGACAAATTGTTCCGAGTTGGCCCTGATATGGAATTGAG
GCCCTGGATGGCTGAAGATCACGAGGTCGTAGACGAACAAACCATAGATGTGGAACTTCGGGACGGTTTAGAGTTTCATG
ACGGTGAACCTGTTACACCAAGAGACGTTAAATTTACCTTTGATTACATGACAGACTGGGGAGTAGGTTATTTTGCCGGT
TTCTTAGATCCTCTAGACGAAGTGGAACTACTTGAAGACGAAACAATTCGCTTCCACTTGGAAGAACCCAATGCAACCTT
CTTGACAAATACTTTAACCCAAATTGTCATCTTACCAAAACACATTTGGGGAGACTTAGTTGAACCAGAAGAGGATCATG
TTTGGGGAGAAATGATGGATGATCCCAGTCAAAGCGAAGGATTATCCCACCCCGATGAATATGAAAATGAAGAAGCCATC
GGAAGTGGACCTTATGAACTGGACTACTGGAACCGAGGTGAAGAAATCAGCCTTGTTCGCAATGAAAATTACTTTGACCA
TCCCGATTACTATGATGCCGATGAAATAGATGTAGAAAGGATTTCCTATATAATTTACGGACACGAAGAAGGTGTTATGG
GCGGCCTTGAACACGAAGAAATTGACCTGGTAGGAGATGCTTACATGTCCGATTACGTGGATAGAGTTGAAGAAATGGAG
CATGTGGAATATTCCGGACACACATCCATCGGTTTCCACTATTTATCCTTCGATCTCAGCGAACCACCTTTTGAAGACCA
CGCCTTTAGAGAAGCAGCTGCCCACTTAATAGATGCCGAAGAACTACTGGATATTGTCCACGGAGGTTACGGAGAACCCG
GTGGTGCAGGTAGAGTAATTTCACCATCAGTCGAGTTTTGGAGAAATCCCGATGTGCCAGAATACCCCTTTGACCCAGAC
CGAGCAGTAGAAATTTTAGAAGACGCAGGTTATGAATTTTACGATGGAGATCTCTATTATCCCGAAGACTAA

Upstream 100 bases:

>100_bases
TAAAGTTTTAAGTAATCAAATTTATTAAGGGGGGGGGATTTCAAAAAGTATTACCGAGACAAAAGACAAGTTTAATCAAA
TTTTTACAGGGGGGTTTAAT

Downstream 100 bases:

>100_bases
ACATGGGCACAACATGAGTAAAACATGAGCATAACAGGGTAAGGGGGCAAACTTGGCCCTCTTATCCTGTTAATCAATAT
AATCAATCCATTTAAGCTGC

Product: extracellular solute-binding protein family 5

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MKGYWWTFVTVLVLSFTLVFTGCGPDDADPAEEEPEEAPDPDRKVEEIVINTTTMDYDPARNEVARWVGDTFETELGVDV
EVQPREFTTLVDSARRDPGESEWQAITLGWSGRIERADPDMFTHTLFHSDQAYDGGNNYHNYESDEYDELAEQARMAFDE
DERQELVYEMQEKLAEDIPMIVLYNEAEHQAQNVERWDNVIQTPEGKYSEWFPYYAEPLTDDDHFRIGYTQDLDTFNPLA
ATTVFEWKLLRLNYDKLFRVGPDMELRPWMAEDHEVVDEQTIDVELRDGLEFHDGEPVTPRDVKFTFDYMTDWGVGYFAG
FLDPLDEVELLEDETIRFHLEEPNATFLTNTLTQIVILPKHIWGDLVEPEEDHVWGEMMDDPSQSEGLSHPDEYENEEAI
GSGPYELDYWNRGEEISLVRNENYFDHPDYYDADEIDVERISYIIYGHEEGVMGGLEHEEIDLVGDAYMSDYVDRVEEME
HVEYSGHTSIGFHYLSFDLSEPPFEDHAFREAAAHLIDAEELLDIVHGGYGEPGGAGRVISPSVEFWRNPDVPEYPFDPD
RAVEILEDAGYEFYDGDLYYPED

Sequences:

>Translated_583_residues
MKGYWWTFVTVLVLSFTLVFTGCGPDDADPAEEEPEEAPDPDRKVEEIVINTTTMDYDPARNEVARWVGDTFETELGVDV
EVQPREFTTLVDSARRDPGESEWQAITLGWSGRIERADPDMFTHTLFHSDQAYDGGNNYHNYESDEYDELAEQARMAFDE
DERQELVYEMQEKLAEDIPMIVLYNEAEHQAQNVERWDNVIQTPEGKYSEWFPYYAEPLTDDDHFRIGYTQDLDTFNPLA
ATTVFEWKLLRLNYDKLFRVGPDMELRPWMAEDHEVVDEQTIDVELRDGLEFHDGEPVTPRDVKFTFDYMTDWGVGYFAG
FLDPLDEVELLEDETIRFHLEEPNATFLTNTLTQIVILPKHIWGDLVEPEEDHVWGEMMDDPSQSEGLSHPDEYENEEAI
GSGPYELDYWNRGEEISLVRNENYFDHPDYYDADEIDVERISYIIYGHEEGVMGGLEHEEIDLVGDAYMSDYVDRVEEME
HVEYSGHTSIGFHYLSFDLSEPPFEDHAFREAAAHLIDAEELLDIVHGGYGEPGGAGRVISPSVEFWRNPDVPEYPFDPD
RAVEILEDAGYEFYDGDLYYPED
>Mature_583_residues
MKGYWWTFVTVLVLSFTLVFTGCGPDDADPAEEEPEEAPDPDRKVEEIVINTTTMDYDPARNEVARWVGDTFETELGVDV
EVQPREFTTLVDSARRDPGESEWQAITLGWSGRIERADPDMFTHTLFHSDQAYDGGNNYHNYESDEYDELAEQARMAFDE
DERQELVYEMQEKLAEDIPMIVLYNEAEHQAQNVERWDNVIQTPEGKYSEWFPYYAEPLTDDDHFRIGYTQDLDTFNPLA
ATTVFEWKLLRLNYDKLFRVGPDMELRPWMAEDHEVVDEQTIDVELRDGLEFHDGEPVTPRDVKFTFDYMTDWGVGYFAG
FLDPLDEVELLEDETIRFHLEEPNATFLTNTLTQIVILPKHIWGDLVEPEEDHVWGEMMDDPSQSEGLSHPDEYENEEAI
GSGPYELDYWNRGEEISLVRNENYFDHPDYYDADEIDVERISYIIYGHEEGVMGGLEHEEIDLVGDAYMSDYVDRVEEME
HVEYSGHTSIGFHYLSFDLSEPPFEDHAFREAAAHLIDAEELLDIVHGGYGEPGGAGRVISPSVEFWRNPDVPEYPFDPD
RAVEILEDAGYEFYDGDLYYPED

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=354, Percent_Identity=22.8813559322034, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1787551, Length=381, Percent_Identity=23.3595800524934, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 67665; Mature: 67665

Theoretical pI: Translated: 3.78; Mature: 3.78

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGYWWTFVTVLVLSFTLVFTGCGPDDADPAEEEPEEAPDPDRKVEEIVINTTTMDYDPA
CCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEEECCCCCC
RNEVARWVGDTFETELGVDVEVQPREFTTLVDSARRDPGESEWQAITLGWSGRIERADPD
HHHHHHHHCCCHHHCCCCCEEECCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCC
MFTHTLFHSDQAYDGGNNYHNYESDEYDELAEQARMAFDEDERQELVYEMQEKLAEDIPM
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCE
IVLYNEAEHQAQNVERWDNVIQTPEGKYSEWFPYYAEPLTDDDHFRIGYTQDLDTFNPLA
EEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHH
ATTVFEWKLLRLNYDKLFRVGPDMELRPWMAEDHEVVDEQTIDVELRDGLEFHDGEPVTP
HHHEEEEEEEEECHHHHHCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCC
RDVKFTFDYMTDWGVGYFAGFLDPLDEVELLEDETIRFHLEEPNATFLTNTLTQIVILPK
CCEEEEEECCHHCCHHHHHHHHCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHEEECH
HIWGDLVEPEEDHVWGEMMDDPSQSEGLSHPDEYENEEAIGSGPYELDYWNRGEEISLVR
HHHCCCCCCCCCCCHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEE
NENYFDHPDYYDADEIDVERISYIIYGHEEGVMGGLEHEEIDLVGDAYMSDYVDRVEEME
CCCCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
HVEYSGHTSIGFHYLSFDLSEPPFEDHAFREAAAHLIDAEELLDIVHGGYGEPGGAGRVI
HHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEE
SPSVEFWRNPDVPEYPFDPDRAVEILEDAGYEFYDGDLYYPED
CCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEECCEEECCCC
>Mature Secondary Structure
MKGYWWTFVTVLVLSFTLVFTGCGPDDADPAEEEPEEAPDPDRKVEEIVINTTTMDYDPA
CCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEEECCCCCC
RNEVARWVGDTFETELGVDVEVQPREFTTLVDSARRDPGESEWQAITLGWSGRIERADPD
HHHHHHHHCCCHHHCCCCCEEECCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCC
MFTHTLFHSDQAYDGGNNYHNYESDEYDELAEQARMAFDEDERQELVYEMQEKLAEDIPM
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCE
IVLYNEAEHQAQNVERWDNVIQTPEGKYSEWFPYYAEPLTDDDHFRIGYTQDLDTFNPLA
EEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHH
ATTVFEWKLLRLNYDKLFRVGPDMELRPWMAEDHEVVDEQTIDVELRDGLEFHDGEPVTP
HHHEEEEEEEEECHHHHHCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCC
RDVKFTFDYMTDWGVGYFAGFLDPLDEVELLEDETIRFHLEEPNATFLTNTLTQIVILPK
CCEEEEEECCHHCCHHHHHHHHCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHEEECH
HIWGDLVEPEEDHVWGEMMDDPSQSEGLSHPDEYENEEAIGSGPYELDYWNRGEEISLVR
HHHCCCCCCCCCCCHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEE
NENYFDHPDYYDADEIDVERISYIIYGHEEGVMGGLEHEEIDLVGDAYMSDYVDRVEEME
CCCCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
HVEYSGHTSIGFHYLSFDLSEPPFEDHAFREAAAHLIDAEELLDIVHGGYGEPGGAGRVI
HHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEE
SPSVEFWRNPDVPEYPFDPDRAVEILEDAGYEFYDGDLYYPED
CCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEECCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]