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The map label for this gene is splB [H]

Identifier: 188586949

GI number: 188586949

Start: 2488798

End: 2489871

Strand: Reverse

Name: splB [H]

Synonym: Nther_2341

Alternate gene names: 188586949

Gene position: 2489871-2488798 (Counterclockwise)

Preceding gene: 188586950

Following gene: 188586948

Centisome position: 78.66

GC content: 39.11

Gene sequence:

>1074_bases
ATGAAAACCCCCACAAAAAAAGTTGACCCCGAGAGTATCATCCCCCAATTCATTCCCAACAGAGTTTATATAGAACAAGG
AGCTCTAGAATATCCGATTGGCAAGGGGATCAAGGAAAAGTTTGACAAGGAAGGAGTTCCTATTAAAATTATTAAAACTC
ATCAAAGGGTGACTGGAATACCGGGTGATACCAGGGAGGAGGGTTATCGGGAGGCTAAACGGACTCTGGTAGTAGGTGTT
AGAAAATCTAAGGACTTTCAAACTTGTAAACCCTCGGCTCATTATCAGCTGCCACTGTCCACTAGCTGTCCCGGCCTATG
CAAGTACTGCTACTTAAATACCACCCTGGGAAAGAAACCATATCTCAGGGTTTATGTCAATATTGACGACATTTTAGCTA
AAGCAAAGAATTATATTGATAAAGGTGCCCCAGAGGAGACGGTTTTTGAGGGAGCTGCTACTTCCGATCCATTACCTGTC
GAGGGTTTGACTGGTAATTTGCGAAAAAGTATAGAATTTTTTGCCCGTGAAGACCTGGGGAGATTTCGTTTTGTTACTAA
ATTTACCAATGTGGATTCACTCCTGGACTTAGAACATAATAAAAAGACTAGAATAAGGTTTAGTTTGAATACTGATCGGG
TTATAAAAGAATACGAACAAGGAACACCGGGTTTGTATCAAAGGATAGAAGCTGCTGGTAAAGTTTACCGGGCTGGATAT
CCCATTGGTTTTATCATTGCCCCAATAATGTTAACTCCTGGCTATCGCAAAGAGTATAGACATCTTTTGGAGAATTTGCG
GGAAGAATTATTAAAATCTGGATTAGATGCTCAAAGTGAAATACCCTTTGAATTGATAACACACAGGTTTACTGCAAGAG
CCAAACAAAATATCGAAGAGATTTTTCCTCATCACGGTTTACCTATGGACGAATCAAAACGTCGATTTAAATACGGTCAA
TTTGGATATGGTAAATACGTCTATCATAAGGAAGATATGGAAGATTTACAAAGATTTTTCAATGACGAAATTAAGAAACT
GTTTCCCGGTGGGGAAATTGTTTATTTTGTTTAA

Upstream 100 bases:

>100_bases
GCTTCTATAGTTAAGTTAGATAATAATTGTGAATAATAAAGATACTCAAATTGAAACTAAATTGAAATGTACTGTCAATT
AAAACAGGAGGTTATAAACC

Downstream 100 bases:

>100_bases
ACTATAGAATGTGATATTATGTAATCAGTATCAATCAGTATTGTGAATTTAAGATACTAATGACTTTTCAAAAGGAGGAA
TTTAGGTGTCAGAAAAGGTG

Product: Radical SAM domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKTPTKKVDPESIIPQFIPNRVYIEQGALEYPIGKGIKEKFDKEGVPIKIIKTHQRVTGIPGDTREEGYREAKRTLVVGV
RKSKDFQTCKPSAHYQLPLSTSCPGLCKYCYLNTTLGKKPYLRVYVNIDDILAKAKNYIDKGAPEETVFEGAATSDPLPV
EGLTGNLRKSIEFFAREDLGRFRFVTKFTNVDSLLDLEHNKKTRIRFSLNTDRVIKEYEQGTPGLYQRIEAAGKVYRAGY
PIGFIIAPIMLTPGYRKEYRHLLENLREELLKSGLDAQSEIPFELITHRFTARAKQNIEEIFPHHGLPMDESKRRFKYGQ
FGYGKYVYHKEDMEDLQRFFNDEIKKLFPGGEIVYFV

Sequences:

>Translated_357_residues
MKTPTKKVDPESIIPQFIPNRVYIEQGALEYPIGKGIKEKFDKEGVPIKIIKTHQRVTGIPGDTREEGYREAKRTLVVGV
RKSKDFQTCKPSAHYQLPLSTSCPGLCKYCYLNTTLGKKPYLRVYVNIDDILAKAKNYIDKGAPEETVFEGAATSDPLPV
EGLTGNLRKSIEFFAREDLGRFRFVTKFTNVDSLLDLEHNKKTRIRFSLNTDRVIKEYEQGTPGLYQRIEAAGKVYRAGY
PIGFIIAPIMLTPGYRKEYRHLLENLREELLKSGLDAQSEIPFELITHRFTARAKQNIEEIFPHHGLPMDESKRRFKYGQ
FGYGKYVYHKEDMEDLQRFFNDEIKKLFPGGEIVYFV
>Mature_357_residues
MKTPTKKVDPESIIPQFIPNRVYIEQGALEYPIGKGIKEKFDKEGVPIKIIKTHQRVTGIPGDTREEGYREAKRTLVVGV
RKSKDFQTCKPSAHYQLPLSTSCPGLCKYCYLNTTLGKKPYLRVYVNIDDILAKAKNYIDKGAPEETVFEGAATSDPLPV
EGLTGNLRKSIEFFAREDLGRFRFVTKFTNVDSLLDLEHNKKTRIRFSLNTDRVIKEYEQGTPGLYQRIEAAGKVYRAGY
PIGFIIAPIMLTPGYRKEYRHLLENLREELLKSGLDAQSEIPFELITHRFTARAKQNIEEIFPHHGLPMDESKRRFKYGQ
FGYGKYVYHKEDMEDLQRFFNDEIKKLFPGGEIVYFV

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 41151; Mature: 41151

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPTKKVDPESIIPQFIPNRVYIEQGALEYPIGKGIKEKFDKEGVPIKIIKTHQRVTGI
CCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCCCCCCEEEEECCHHHCCC
PGDTREEGYREAKRTLVVGVRKSKDFQTCKPSAHYQLPLSTSCPGLCKYCYLNTTLGKKP
CCCCHHHHHHHHHHEEEEEEECCCCCCCCCCCCCEECCCCCCCCHHHHHHEEECCCCCCC
YLRVYVNIDDILAKAKNYIDKGAPEETVFEGAATSDPLPVEGLTGNLRKSIEFFAREDLG
EEEEEECHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCH
RFRFVTKFTNVDSLLDLEHNKKTRIRFSLNTDRVIKEYEQGTPGLYQRIEAAGKVYRAGY
HHHHHHHHCCHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHEECCC
PIGFIIAPIMLTPGYRKEYRHLLENLREELLKSGLDAQSEIPFELITHRFTARAKQNIEE
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IFPHHGLPMDESKRRFKYGQFGYGKYVYHKEDMEDLQRFFNDEIKKLFPGGEIVYFV
HCCCCCCCCCCCHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MKTPTKKVDPESIIPQFIPNRVYIEQGALEYPIGKGIKEKFDKEGVPIKIIKTHQRVTGI
CCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCCCCCCEEEEECCHHHCCC
PGDTREEGYREAKRTLVVGVRKSKDFQTCKPSAHYQLPLSTSCPGLCKYCYLNTTLGKKP
CCCCHHHHHHHHHHEEEEEEECCCCCCCCCCCCCEECCCCCCCCHHHHHHEEECCCCCCC
YLRVYVNIDDILAKAKNYIDKGAPEETVFEGAATSDPLPVEGLTGNLRKSIEFFAREDLG
EEEEEECHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCH
RFRFVTKFTNVDSLLDLEHNKKTRIRFSLNTDRVIKEYEQGTPGLYQRIEAAGKVYRAGY
HHHHHHHHCCHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHEECCC
PIGFIIAPIMLTPGYRKEYRHLLENLREELLKSGLDAQSEIPFELITHRFTARAKQNIEE
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IFPHHGLPMDESKRRFKYGQFGYGKYVYHKEDMEDLQRFFNDEIKKLFPGGEIVYFV
HCCCCCCCCCCCHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]