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The map label for this gene is lutR [H]

Identifier: 188586850

GI number: 188586850

Start: 2377617

End: 2378300

Strand: Reverse

Name: lutR [H]

Synonym: Nther_2241

Alternate gene names: 188586850

Gene position: 2378300-2377617 (Counterclockwise)

Preceding gene: 188586851

Following gene: 188586848

Centisome position: 75.13

GC content: 34.5

Gene sequence:

>684_bases
ATGGAAGATTGGAATATTAAAAAATTAAAAACAACTAGCTTAAGTGATAGGGTAATTGATCAAATTATAGATTTAATAGT
TCAAGGTAAATTGAAACCTGGTAATAAACTTCCTTCTGAAAGAGAATTGGTGGAGTTATTTGGTGTTAGTCGAAGTTCCA
TAAGAGAGGCATTAAAAACATTGGAAAAAATCAATCTGCTTAAATCTGTCCCTGGAAGAGGTGTCTATCTTTCCAGTGAG
GAAGATGAGGGATCAGTTAGCGCACTGGCTTATCCCCTTTTACTGACAAATGATATAGAAGAACTATTTGAAGCTCGTGA
ATTAATCCAAGTGGAAATGGCAAGAAAAGCGGCGAAAAGGGCAACTGAAGAAGATATTAAGAATATTAGAGAAGTAATCA
GAAAAGCTTCACAAGCTGAAACGAAATCGGAAAAAGCCGAACTGGACGTAGAATTTGATTTAAATTTAGCCAAAGCTGCA
AGGAATTCGGTCCTTTTAAAATTTTTAATTAGTATTCAAGAAGTGCTCAGATTAACTCAACTAAAATTTATTACCGAAGA
AAGATACACAAAATCAATAAATGATCACACAAAAATCGTTGACTTTGTTGAAAAAGGAAAACCAGAGGAAGCCGCTGAAG
CCATGAGAGTTCACCTGATCAGTGTTAAAGACGATATCAACTAA

Upstream 100 bases:

>100_bases
TAGAATGGTAAAAGGTTTGAAGGAAAAATTATGGAATTATTTATTAAGTAGGTTGTAGGTAGTTGTTAGTGGTTAGTGCC
AACAAAAGGAGTGAAGTTTA

Downstream 100 bases:

>100_bases
ACCGACTAAATCCTTATATTCGCGAAAACGCAAAAAACGGTAGCATTGACGCTACCGCTTTTTATGTATAGATTATGTCG
ATAAATATGTAAAAAATAGC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MEDWNIKKLKTTSLSDRVIDQIIDLIVQGKLKPGNKLPSERELVELFGVSRSSIREALKTLEKINLLKSVPGRGVYLSSE
EDEGSVSALAYPLLLTNDIEELFEARELIQVEMARKAAKRATEEDIKNIREVIRKASQAETKSEKAELDVEFDLNLAKAA
RNSVLLKFLISIQEVLRLTQLKFITEERYTKSINDHTKIVDFVEKGKPEEAAEAMRVHLISVKDDIN

Sequences:

>Translated_227_residues
MEDWNIKKLKTTSLSDRVIDQIIDLIVQGKLKPGNKLPSERELVELFGVSRSSIREALKTLEKINLLKSVPGRGVYLSSE
EDEGSVSALAYPLLLTNDIEELFEARELIQVEMARKAAKRATEEDIKNIREVIRKASQAETKSEKAELDVEFDLNLAKAA
RNSVLLKFLISIQEVLRLTQLKFITEERYTKSINDHTKIVDFVEKGKPEEAAEAMRVHLISVKDDIN
>Mature_227_residues
MEDWNIKKLKTTSLSDRVIDQIIDLIVQGKLKPGNKLPSERELVELFGVSRSSIREALKTLEKINLLKSVPGRGVYLSSE
EDEGSVSALAYPLLLTNDIEELFEARELIQVEMARKAAKRATEEDIKNIREVIRKASQAETKSEKAELDVEFDLNLAKAA
RNSVLLKFLISIQEVLRLTQLKFITEERYTKSINDHTKIVDFVEKGKPEEAAEAMRVHLISVKDDIN

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=216, Percent_Identity=32.4074074074074, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1786303, Length=230, Percent_Identity=33.4782608695652, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI1790780, Length=215, Percent_Identity=33.0232558139535, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1789621, Length=224, Percent_Identity=28.125, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1789353, Length=223, Percent_Identity=29.5964125560538, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI48994955, Length=212, Percent_Identity=25.4716981132075, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082208, Length=203, Percent_Identity=27.5862068965517, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25776; Mature: 25776

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDWNIKKLKTTSLSDRVIDQIIDLIVQGKLKPGNKLPSERELVELFGVSRSSIREALKT
CCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
LEKINLLKSVPGRGVYLSSEEDEGSVSALAYPLLLTNDIEELFEARELIQVEMARKAAKR
HHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATEEDIKNIREVIRKASQAETKSEKAELDVEFDLNLAKAARNSVLLKFLISIQEVLRLTQ
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKFITEERYTKSINDHTKIVDFVEKGKPEEAAEAMRVHLISVKDDIN
HHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEDWNIKKLKTTSLSDRVIDQIIDLIVQGKLKPGNKLPSERELVELFGVSRSSIREALKT
CCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
LEKINLLKSVPGRGVYLSSEEDEGSVSALAYPLLLTNDIEELFEARELIQVEMARKAAKR
HHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATEEDIKNIREVIRKASQAETKSEKAELDVEFDLNLAKAARNSVLLKFLISIQEVLRLTQ
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKFITEERYTKSINDHTKIVDFVEKGKPEEAAEAMRVHLISVKDDIN
HHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]