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The map label for this gene is lplA [H]

Identifier: 188586793

GI number: 188586793

Start: 2313705

End: 2314694

Strand: Reverse

Name: lplA [H]

Synonym: Nther_2184

Alternate gene names: 188586793

Gene position: 2314694-2313705 (Counterclockwise)

Preceding gene: 188586794

Following gene: 188586791

Centisome position: 73.12

GC content: 33.84

Gene sequence:

>990_bases
ATGAGATACATACAAACGGACTGGAACATACCGTATTACAACTTTGCTCTTGAGGAATATTTAATAAATGAAGCACCGGA
AGACAGCTATTTGTTTTTTTACATTCACAAACCATCTATTATCGTAGGTAAGTATCAGAACACTATTGAAGAAATTAATA
AAGAGTTTGTTGATGAGCACGATATCATAGTGGCTAGAAGAATTTCTGGCGGCGGGGCAGTTTACCATGATGAAGGTAAT
TTGAATTTTTCTTTTGTTCAAAAAGCGGACAAGGAAGATGTTAATAATTTTGAGAAATTTACGAGACCGATAATAGAGGC
CCTTAATGAACTAGGGGTAAAAGCTGAACTTTCCGGGCGAAATGATATTCTGGTAGACGGCAAGAAAATTTCAGGAAACG
CCCAGTATTATGCCAATGGAAGGATATTAAGCCATGGGACCTTATTATTTAACTCCGAAATGTCCAATTTAACCAAAGCT
CTAAAGGCAAATGACCTTAAGATTAAGTCAAAAGGGGTAAAATCAGTTAAAAGTAGAGTGGCAAACATTAAAGATTATAT
GGATCAAGATATGTCAATTTATGAGTTGAAAAACTATCTTCTAGAATATATTTCTAATACTTTAGGTGTTGAGGAATATG
TCTTAAGTCAAGAAGAGCTTAAGAAAATTGAAGACGCTGCAGAAAATAAATTTAGTAAATGGGAATGGAATTGGGGTAAG
TCCCCGGCTTTTGAAATTAGTAAAGTTGATAAATTCTCCTGTGGTTTGATCAATGTAAAATTAAATGTTCGCAAAGGTAA
AATTAAAGACTGTAAAATTTACGGTGATTTTTTCACCAGAAGAGAGCTTGAAGAACTGGAAAACTTCTTATGTAATCAAA
CTTATGAACAAGAAAGCCTAAAAAAGGCTTTACAAGAGAAAGAGATTGACTTGGAAGATTATTTTCAAGGGATTGATCCC
GAGGAGTTTGCAGAATTTATTACAACATAA

Upstream 100 bases:

>100_bases
TTACCGAGATAACTTGTGATTGATAAGCGTTAACACATTGAACTAATTAATGTTATTATAATAGTAATAATAAACTTGCA
TAGATCTGGAGGGATGAAAA

Downstream 100 bases:

>100_bases
TATGTTTGCTAATATTTTTGGTTAAGTAAATCGGGTCAAGTAAAAAAGTGCAAATTAACTAAATTAGTACGATAAACTAA
AGCGAGGCAGACTAAATTAG

Product: lipoyltransferase and lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MRYIQTDWNIPYYNFALEEYLINEAPEDSYLFFYIHKPSIIVGKYQNTIEEINKEFVDEHDIIVARRISGGGAVYHDEGN
LNFSFVQKADKEDVNNFEKFTRPIIEALNELGVKAELSGRNDILVDGKKISGNAQYYANGRILSHGTLLFNSEMSNLTKA
LKANDLKIKSKGVKSVKSRVANIKDYMDQDMSIYELKNYLLEYISNTLGVEEYVLSQEELKKIEDAAENKFSKWEWNWGK
SPAFEISKVDKFSCGLINVKLNVRKGKIKDCKIYGDFFTRRELEELENFLCNQTYEQESLKKALQEKEIDLEDYFQGIDP
EEFAEFITT

Sequences:

>Translated_329_residues
MRYIQTDWNIPYYNFALEEYLINEAPEDSYLFFYIHKPSIIVGKYQNTIEEINKEFVDEHDIIVARRISGGGAVYHDEGN
LNFSFVQKADKEDVNNFEKFTRPIIEALNELGVKAELSGRNDILVDGKKISGNAQYYANGRILSHGTLLFNSEMSNLTKA
LKANDLKIKSKGVKSVKSRVANIKDYMDQDMSIYELKNYLLEYISNTLGVEEYVLSQEELKKIEDAAENKFSKWEWNWGK
SPAFEISKVDKFSCGLINVKLNVRKGKIKDCKIYGDFFTRRELEELENFLCNQTYEQESLKKALQEKEIDLEDYFQGIDP
EEFAEFITT
>Mature_329_residues
MRYIQTDWNIPYYNFALEEYLINEAPEDSYLFFYIHKPSIIVGKYQNTIEEINKEFVDEHDIIVARRISGGGAVYHDEGN
LNFSFVQKADKEDVNNFEKFTRPIIEALNELGVKAELSGRNDILVDGKKISGNAQYYANGRILSHGTLLFNSEMSNLTKA
LKANDLKIKSKGVKSVKSRVANIKDYMDQDMSIYELKNYLLEYISNTLGVEEYVLSQEELKKIEDAAENKFSKWEWNWGK
SPAFEISKVDKFSCGLINVKLNVRKGKIKDCKIYGDFFTRRELEELENFLCNQTYEQESLKKALQEKEIDLEDYFQGIDP
EEFAEFITT

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=274, Percent_Identity=29.5620437956204, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI21729884, Length=274, Percent_Identity=29.5620437956204, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI21729882, Length=274, Percent_Identity=29.5620437956204, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI21729880, Length=274, Percent_Identity=29.5620437956204, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI21729878, Length=274, Percent_Identity=29.5620437956204, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1790846, Length=323, Percent_Identity=32.1981424148607, Blast_Score=165, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71981909, Length=255, Percent_Identity=31.3725490196078, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322415, Length=284, Percent_Identity=31.3380281690141, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28573464, Length=256, Percent_Identity=30.46875, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38230; Mature: 38230

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYIQTDWNIPYYNFALEEYLINEAPEDSYLFFYIHKPSIIVGKYQNTIEEINKEFVDEH
CCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEHHHHHHHHHHHHHCCCC
DIIVARRISGGGAVYHDEGNLNFSFVQKADKEDVNNFEKFTRPIIEALNELGVKAELSGR
CEEEEEEECCCCEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
NDILVDGKKISGNAQYYANGRILSHGTLLFNSEMSNLTKALKANDLKIKSKGVKSVKSRV
CCEEEECEEECCCEEEEECCEEEECCEEEECCHHHHHHHHHHCCCCEEHHHHHHHHHHHH
ANIKDYMDQDMSIYELKNYLLEYISNTLGVEEYVLSQEELKKIEDAAENKFSKWEWNWGK
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCC
SPAFEISKVDKFSCGLINVKLNVRKGKIKDCKIYGDFFTRRELEELENFLCNQTYEQESL
CCCCCHHCCCCCCCEEEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHH
KKALQEKEIDLEDYFQGIDPEEFAEFITT
HHHHHHCCCCHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MRYIQTDWNIPYYNFALEEYLINEAPEDSYLFFYIHKPSIIVGKYQNTIEEINKEFVDEH
CCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEHHHHHHHHHHHHHCCCC
DIIVARRISGGGAVYHDEGNLNFSFVQKADKEDVNNFEKFTRPIIEALNELGVKAELSGR
CEEEEEEECCCCEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
NDILVDGKKISGNAQYYANGRILSHGTLLFNSEMSNLTKALKANDLKIKSKGVKSVKSRV
CCEEEECEEECCCEEEEECCEEEECCEEEECCHHHHHHHHHHCCCCEEHHHHHHHHHHHH
ANIKDYMDQDMSIYELKNYLLEYISNTLGVEEYVLSQEELKKIEDAAENKFSKWEWNWGK
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCC
SPAFEISKVDKFSCGLINVKLNVRKGKIKDCKIYGDFFTRRELEELENFLCNQTYEQESL
CCCCCHHCCCCCCCEEEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHH
KKALQEKEIDLEDYFQGIDPEEFAEFITT
HHHHHHCCCCHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]