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The map label for this gene is trmE [C]

Identifier: 188586779

GI number: 188586779

Start: 2296058

End: 2297032

Strand: Reverse

Name: trmE [C]

Synonym: Nther_2170

Alternate gene names: 188586779

Gene position: 2297032-2296058 (Counterclockwise)

Preceding gene: 188586780

Following gene: 188586778

Centisome position: 72.56

GC content: 41.44

Gene sequence:

>975_bases
ATGCCTGCTAATCTTCCACCACAATATTATGAGGCTGAAGAAGCGTATCGTAAAGCTACAACTCCTGAAGACAAAATGGA
AGCATTGAAAACTATGTTAAAGGAAATTCCCAAACATAAGGGTACGGAAAAACTTCAAGCTGATATTAAAAGAAGGATGG
CTCGCCTCCGAGAAGAAGAGGACAAGAAAAAGAAATCTTATAAAAGTACGTACAACCCTTTTCATATTGAAAAGCAGGGA
GCCGGACAGATAGTTTTAGTTGGTTATCCTAATACGGGGAAATCCTCTTTAGTAGCAACATTGACACGTGCTAAAGCCAA
GGTGGCAGATTACCCCTACTCTACAGCTTTACCTCTTCCGGGAATGATGCCTTATCGGGACACATGGGTACAATTAGTCG
ACACACCTCCGGTGATGGAAGATAGTATTGCTTCGGAATTAATCGGTACTATCAGAAATGCCGATGCTTTACTTATTATG
ATTGATGCTGGTTCTGATGATTGTTTGGATCAACTGGAAGGTTGTTTAAACTTACTACAGGATAAAGGTGTGATTGACCT
GTCGGAAGATGGGGAAATTGTCGCTCCTATACCTTATATGGTGGTGGCTATGAAAATGGATTTGCCGGGGGCAAAGGAAA
ATATGGATCTAATAAAGGAGTTCAATCCTGAACTGACAATTTGGGAGGCAGCTACAGATGGTACAGGTCTAGATCAATTA
AGAGAAGAGTTATTTGAAATTTTAGAAGTTATTCGAGTATATGGTAAACCTGCTGGAAAACCCGTAGACACCGAACGTCC
ATTTATTTTAAGAAAGGGAAGTACAGTTTTGGACTTTGCCGAAACTGTCCATAGGGACTTTCCCGATAAACTGCAAAGTG
CTTTGGTATGGGGGTCAGCACGTTTTGATGGACAGGCGGTACCCCAGGATCATGTCTTAGAAGACGGGGATATAGTGGAA
TTACAAATCAGGTGA

Upstream 100 bases:

>100_bases
TAATGGGACAGTATTTGTTGTATTTGATTTTTTATTAACTGTGTAACTGTGATGCTCTCTAAAGAAGCTTCAAAATTGAT
TGTTTAAAAGGGGGGTTATA

Downstream 100 bases:

>100_bases
ATTGATATATATATCAATTATATAGTGGCAGAAAATTAAATTTATCATTAATATTGATTAAAAGGTGAGGTGATGACAAT
GAATGATCAACATGTGGAAG

Product: GTP-binding protein HSR1-related

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MPANLPPQYYEAEEAYRKATTPEDKMEALKTMLKEIPKHKGTEKLQADIKRRMARLREEEDKKKKSYKSTYNPFHIEKQG
AGQIVLVGYPNTGKSSLVATLTRAKAKVADYPYSTALPLPGMMPYRDTWVQLVDTPPVMEDSIASELIGTIRNADALLIM
IDAGSDDCLDQLEGCLNLLQDKGVIDLSEDGEIVAPIPYMVVAMKMDLPGAKENMDLIKEFNPELTIWEAATDGTGLDQL
REELFEILEVIRVYGKPAGKPVDTERPFILRKGSTVLDFAETVHRDFPDKLQSALVWGSARFDGQAVPQDHVLEDGDIVE
LQIR

Sequences:

>Translated_324_residues
MPANLPPQYYEAEEAYRKATTPEDKMEALKTMLKEIPKHKGTEKLQADIKRRMARLREEEDKKKKSYKSTYNPFHIEKQG
AGQIVLVGYPNTGKSSLVATLTRAKAKVADYPYSTALPLPGMMPYRDTWVQLVDTPPVMEDSIASELIGTIRNADALLIM
IDAGSDDCLDQLEGCLNLLQDKGVIDLSEDGEIVAPIPYMVVAMKMDLPGAKENMDLIKEFNPELTIWEAATDGTGLDQL
REELFEILEVIRVYGKPAGKPVDTERPFILRKGSTVLDFAETVHRDFPDKLQSALVWGSARFDGQAVPQDHVLEDGDIVE
LQIR
>Mature_323_residues
PANLPPQYYEAEEAYRKATTPEDKMEALKTMLKEIPKHKGTEKLQADIKRRMARLREEEDKKKKSYKSTYNPFHIEKQGA
GQIVLVGYPNTGKSSLVATLTRAKAKVADYPYSTALPLPGMMPYRDTWVQLVDTPPVMEDSIASELIGTIRNADALLIMI
DAGSDDCLDQLEGCLNLLQDKGVIDLSEDGEIVAPIPYMVVAMKMDLPGAKENMDLIKEFNPELTIWEAATDGTGLDQLR
EELFEILEVIRVYGKPAGKPVDTERPFILRKGSTVLDFAETVHRDFPDKLQSALVWGSARFDGQAVPQDHVLEDGDIVEL
QIR

Specific function: Exhibits A Very High Intrinsic Gtpase Hydrolysis Rate. Involved In The Biosynthesis Of The Hypermodified Nucleoside 5- Methylaminomethyl-2-Thiouridine, Which Is Found In The Wobble Position Of Some Trnas. [C]

COG id: COG1163

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasmic; may also be associated with the inner membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI4758796, Length=361, Percent_Identity=26.0387811634349, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI4557537, Length=146, Percent_Identity=28.7671232876712, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI24308117, Length=173, Percent_Identity=27.7456647398844, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71981008, Length=149, Percent_Identity=26.1744966442953, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17555344, Length=173, Percent_Identity=31.7919075144509, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508313, Length=177, Percent_Identity=30.5084745762712, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321612, Length=146, Percent_Identity=30.1369863013699, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6319281, Length=152, Percent_Identity=24.3421052631579, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17981711, Length=366, Percent_Identity=25.1366120218579, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356473, Length=146, Percent_Identity=28.7671232876712, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20129375, Length=195, Percent_Identity=31.2820512820513, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006074
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004095 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF02824 TGS [H]

EC number: NA

Molecular weight: Translated: 36266; Mature: 36135

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANLPPQYYEAEEAYRKATTPEDKMEALKTMLKEIPKHKGTEKLQADIKRRMARLREEE
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DKKKKSYKSTYNPFHIEKQGAGQIVLVGYPNTGKSSLVATLTRAKAKVADYPYSTALPLP
HHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
GMMPYRDTWVQLVDTPPVMEDSIASELIGTIRNADALLIMIDAGSDDCLDQLEGCLNLLQ
CCCCCHHHHHEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHH
DKGVIDLSEDGEIVAPIPYMVVAMKMDLPGAKENMDLIKEFNPELTIWEAATDGTGLDQL
CCCCEEECCCCCEEECCHHEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHH
REELFEILEVIRVYGKPAGKPVDTERPFILRKGSTVLDFAETVHRDFPDKLQSALVWGSA
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHCCC
RFDGQAVPQDHVLEDGDIVELQIR
CCCCCCCCHHHCCCCCCEEEEEEC
>Mature Secondary Structure 
PANLPPQYYEAEEAYRKATTPEDKMEALKTMLKEIPKHKGTEKLQADIKRRMARLREEE
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DKKKKSYKSTYNPFHIEKQGAGQIVLVGYPNTGKSSLVATLTRAKAKVADYPYSTALPLP
HHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
GMMPYRDTWVQLVDTPPVMEDSIASELIGTIRNADALLIMIDAGSDDCLDQLEGCLNLLQ
CCCCCHHHHHEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHH
DKGVIDLSEDGEIVAPIPYMVVAMKMDLPGAKENMDLIKEFNPELTIWEAATDGTGLDQL
CCCCEEECCCCCEEECCHHEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHH
REELFEILEVIRVYGKPAGKPVDTERPFILRKGSTVLDFAETVHRDFPDKLQSALVWGSA
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHCCC
RFDGQAVPQDHVLEDGDIVELQIR
CCCCCCCCHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]