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The map label for this gene is Hgd [H]

Identifier: 188586770

GI number: 188586770

Start: 2287542

End: 2288441

Strand: Reverse

Name: Hgd [H]

Synonym: Nther_2161

Alternate gene names: 188586770

Gene position: 2288441-2287542 (Counterclockwise)

Preceding gene: 188586771

Following gene: 188586769

Centisome position: 72.29

GC content: 39.89

Gene sequence:

>900_bases
ATGAAAGTAGGATTTATCGGATTAGGTGTTATGGGAAAGAGGATGGCTCAGAATATATTAAAAAATGATTATTCTATGAT
AGTTTTTGATGTTGACGAAAATGTCGTACAAGAATTCGTAGATCAAGGTGCGGAGAAAGCTAGTTCACCAGCGGAAGCTG
CTGCTACTGCAGACGTTGTTCTTACATCTCTTCCTAATGACAGTATCGTTAAAAAAGTTATCCTTGGCGAAAGTGGAGTT
TTAGAAGGTGCTAAAGAAAACACTGTTTTAATTGATTTAAGCAGTATTCCGCCAAAAGCTATTCGAGAAGTTGCAGATCA
AGCAACCAAACAAGGTGTAGAAGTTATCGATGCTCCTGTAAGTGGTGGGGCAGCTGGAGCAGAAAAAGGGACGCTGACAG
TAATGGTAGGTGGAAAATCTGATGTAGTTGACCAAGCGCGACCGGTATTGAACTGTATCTGCAATAAAATATTCCATGTT
GGAGATGTGGGTGCAGGAGATACCTTGAAGCTTGTTAACAATTTAATGTTAGGCGCTAATATGTTGATTGCTTCTGAAGC
AATGGCTTTGGGTACAAAAGCCGGCTTGGATCCTGACGTGCTATTCGAAGTTATTAGTGAAAGTTCTGGTAGATCCTATG
CTCTAGAGGCTAAATATCCCAAATTCATTTCTCAAGGCAATTTTGACCCTGGGTTCTCTATTGATCTGGAATACAAAGAT
TTAGAGTTAGCGATATCAACGGCTAAAGATATGAATATGCCCTTGATAATGGGTAATTTGACGCAACAGCTTTACGAAGC
TGCCAGATGTGAAGGTCTAGGAGACAGAGATATCTCTGCCATGATTAATATGTACGAAAAATGGACTGGAACAAAAGTCA
GAAAGGATGACGAGAAATAA

Upstream 100 bases:

>100_bases
ATAGAAATTTTGGCATAGAAGTTGCACAAACTATAGTACAGAAAATGTAAGGGAATGCAGAAAACCTAACAAAAAAATTA
CAATTTTGGAGGGATATTAT

Downstream 100 bases:

>100_bases
TGAGCAATTTAGTTGATCAGGTTAAATCTCAAATGCCACTAACTTTGGGTGAGCTAATTAATATAGCCCGAGAAAATAAT
AGCAGAGTAGTAGATGTGGT

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKVGFIGLGVMGKRMAQNILKNDYSMIVFDVDENVVQEFVDQGAEKASSPAEAAATADVVLTSLPNDSIVKKVILGESGV
LEGAKENTVLIDLSSIPPKAIREVADQATKQGVEVIDAPVSGGAAGAEKGTLTVMVGGKSDVVDQARPVLNCICNKIFHV
GDVGAGDTLKLVNNLMLGANMLIASEAMALGTKAGLDPDVLFEVISESSGRSYALEAKYPKFISQGNFDPGFSIDLEYKD
LELAISTAKDMNMPLIMGNLTQQLYEAARCEGLGDRDISAMINMYEKWTGTKVRKDDEK

Sequences:

>Translated_299_residues
MKVGFIGLGVMGKRMAQNILKNDYSMIVFDVDENVVQEFVDQGAEKASSPAEAAATADVVLTSLPNDSIVKKVILGESGV
LEGAKENTVLIDLSSIPPKAIREVADQATKQGVEVIDAPVSGGAAGAEKGTLTVMVGGKSDVVDQARPVLNCICNKIFHV
GDVGAGDTLKLVNNLMLGANMLIASEAMALGTKAGLDPDVLFEVISESSGRSYALEAKYPKFISQGNFDPGFSIDLEYKD
LELAISTAKDMNMPLIMGNLTQQLYEAARCEGLGDRDISAMINMYEKWTGTKVRKDDEK
>Mature_299_residues
MKVGFIGLGVMGKRMAQNILKNDYSMIVFDVDENVVQEFVDQGAEKASSPAEAAATADVVLTSLPNDSIVKKVILGESGV
LEGAKENTVLIDLSSIPPKAIREVADQATKQGVEVIDAPVSGGAAGAEKGTLTVMVGGKSDVVDQARPVLNCICNKIFHV
GDVGAGDTLKLVNNLMLGANMLIASEAMALGTKAGLDPDVLFEVISESSGRSYALEAKYPKFISQGNFDPGFSIDLEYKD
LELAISTAKDMNMPLIMGNLTQQLYEAARCEGLGDRDISAMINMYEKWTGTKVRKDDEK

Specific function: Catalyzes the conversion of 2-formylglutarate to (S)-2- hydroxymethylglutarate. Has very low activity with (S)-3- hydroxyisobutyrate [H]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=36.7697594501718, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI40556376, Length=284, Percent_Identity=27.8169014084507, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI145693186, Length=295, Percent_Identity=42.0338983050847, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1790315, Length=280, Percent_Identity=34.6428571428571, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1786719, Length=293, Percent_Identity=32.0819112627986, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1789092, Length=291, Percent_Identity=31.2714776632302, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17557316, Length=270, Percent_Identity=38.8888888888889, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24655230, Length=291, Percent_Identity=36.426116838488, Blast_Score=187, Evalue=6e-48,
Organism=Drosophila melanogaster, GI19922568, Length=291, Percent_Identity=36.426116838488, Blast_Score=187, Evalue=6e-48,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=22.5, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.291 [H]

Molecular weight: Translated: 31843; Mature: 31843

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGFIGLGVMGKRMAQNILKNDYSMIVFDVDENVVQEFVDQGAEKASSPAEAAATADVV
CEEEEEEHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LTSLPNDSIVKKVILGESGVLEGAKENTVLIDLSSIPPKAIREVADQATKQGVEVIDAPV
EECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCC
SGGAAGAEKGTLTVMVGGKSDVVDQARPVLNCICNKIFHVGDVGAGDTLKLVNNLMLGAN
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCH
MLIASEAMALGTKAGLDPDVLFEVISESSGRSYALEAKYPKFISQGNFDPGFSIDLEYKD
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCCCCEEEEEECC
LELAISTAKDMNMPLIMGNLTQQLYEAARCEGLGDRDISAMINMYEKWTGTKVRKDDEK
CEEEEEHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKVGFIGLGVMGKRMAQNILKNDYSMIVFDVDENVVQEFVDQGAEKASSPAEAAATADVV
CEEEEEEHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LTSLPNDSIVKKVILGESGVLEGAKENTVLIDLSSIPPKAIREVADQATKQGVEVIDAPV
EECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCC
SGGAAGAEKGTLTVMVGGKSDVVDQARPVLNCICNKIFHVGDVGAGDTLKLVNNLMLGAN
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCH
MLIASEAMALGTKAGLDPDVLFEVISESSGRSYALEAKYPKFISQGNFDPGFSIDLEYKD
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCCCCEEEEEECC
LELAISTAKDMNMPLIMGNLTQQLYEAARCEGLGDRDISAMINMYEKWTGTKVRKDDEK
CEEEEEHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA