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The map label for this gene is mii [H]

Identifier: 188586765

GI number: 188586765

Start: 2281260

End: 2282396

Strand: Reverse

Name: mii [H]

Synonym: Nther_2156

Alternate gene names: 188586765

Gene position: 2282396-2281260 (Counterclockwise)

Preceding gene: 188586766

Following gene: 188586764

Centisome position: 72.1

GC content: 39.75

Gene sequence:

>1137_bases
GTGGATCAGGAAAAAGTCCCAACAACTATTATGAGAGGCGGCACGAGTAAAGCCATTTTTCTTAAGGATAAAGATCTTCC
GACAAATCAGGAAGAAAGAGATAATTTGATTCTAAGAATTTTTGGAAGCCCCGATCCAAGGCAAATTGATGGCCTTGGGG
GGGCAGACCCTCTAACAAGTAAACTTGCAATTATTGGACCACCCACTAGAGAAGATGCTGATATTGATTATACTTTTGGG
CAAGTTTCATATACTGCTGCCAAAATTGATTATTCCGGTAATTGCGGGAATGTATCCTCAGCTGTAGGACCGTTTGCAAT
AGATAAAGGTTATGTCCAAGCTGAAGAACCATATACTACTGTGAGAGTTCATAACACAAATACTAACAAGATTTTGATTG
AGAAAGTACCTGTTGTAGATGGCTTATCCAAAGTAATTGGTGATTATCAAATTGATGGTGTTCCTGGGCAAGGTGCTCCA
ATATCTATAGACTTTTCCGATACTGCCGGTGCAAAAACAGGTGAATTACTTCCTACAGGCGAGGAAGTGAACAAAATTTC
GACTGAATCTTGTGGAGAGATTGAGGCATCTTTAGTTGATGCTGGTAACCCCATGGTTTTTGTTAGAGCTGAGGACTTGG
GTTTACGAGGAAATGAAACCCCCGAAGAAATTGACAATAATGAAGAAGCTTTAAAAACCCTTGAAGAAATAAGGGGAAAA
GCAGCTGTAATGATGGGAATTGAAACTGATTGGAAACAAGCTGAACAAAATAATCCGGCTTTTCCAATGGTAGCTTTTGT
ATCACCAGCTTCAGATTCACAACAGGAAAGTGGCATAGATTTCAATTCCCGTTTGATGTTTATGCAGGTTATGCACAAAA
CTTATGCTGGTACTGGCACCATCTGTACGGGTTCAGCAGCTATGATTAAAGGCACCATTGTTAATCAGGTTATGAGTTCC
AAAAAAGACCAAGATGAAGCAACTATTAAGATCGGACATCCAGCAGGATTTATTGAAATTGAAGTCCGGGTCGATGAGGA
TGAACAAAATGGAAATTGGATATTGAACAAAGCTGCTATTAATCGGACAGCTCGAAGAATTATGGATGGGAACTGTTATA
TACCAAAAGAAGGCTAG

Upstream 100 bases:

>100_bases
CAATAAATAAGAAAAGAGATAAGGAACTGTTAAGTTATTAAATTCCTTCCGGTAACTATTTATCAATCGTTTCAAAGGCC
GGAACTAAGGAGGGGTTAGT

Downstream 100 bases:

>100_bases
GTGAGTTTCATCATGAGTGTATTTGATTACCTGGTAATTGATATTGGAAGTACTTTTTTAAAACAAAGGCTTTTTAAGGA
CAGTTCATTAGTGGCAACAA

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MDQEKVPTTIMRGGTSKAIFLKDKDLPTNQEERDNLILRIFGSPDPRQIDGLGGADPLTSKLAIIGPPTREDADIDYTFG
QVSYTAAKIDYSGNCGNVSSAVGPFAIDKGYVQAEEPYTTVRVHNTNTNKILIEKVPVVDGLSKVIGDYQIDGVPGQGAP
ISIDFSDTAGAKTGELLPTGEEVNKISTESCGEIEASLVDAGNPMVFVRAEDLGLRGNETPEEIDNNEEALKTLEEIRGK
AAVMMGIETDWKQAEQNNPAFPMVAFVSPASDSQQESGIDFNSRLMFMQVMHKTYAGTGTICTGSAAMIKGTIVNQVMSS
KKDQDEATIKIGHPAGFIEIEVRVDEDEQNGNWILNKAAINRTARRIMDGNCYIPKEG

Sequences:

>Translated_378_residues
MDQEKVPTTIMRGGTSKAIFLKDKDLPTNQEERDNLILRIFGSPDPRQIDGLGGADPLTSKLAIIGPPTREDADIDYTFG
QVSYTAAKIDYSGNCGNVSSAVGPFAIDKGYVQAEEPYTTVRVHNTNTNKILIEKVPVVDGLSKVIGDYQIDGVPGQGAP
ISIDFSDTAGAKTGELLPTGEEVNKISTESCGEIEASLVDAGNPMVFVRAEDLGLRGNETPEEIDNNEEALKTLEEIRGK
AAVMMGIETDWKQAEQNNPAFPMVAFVSPASDSQQESGIDFNSRLMFMQVMHKTYAGTGTICTGSAAMIKGTIVNQVMSS
KKDQDEATIKIGHPAGFIEIEVRVDEDEQNGNWILNKAAINRTARRIMDGNCYIPKEG
>Mature_378_residues
MDQEKVPTTIMRGGTSKAIFLKDKDLPTNQEERDNLILRIFGSPDPRQIDGLGGADPLTSKLAIIGPPTREDADIDYTFG
QVSYTAAKIDYSGNCGNVSSAVGPFAIDKGYVQAEEPYTTVRVHNTNTNKILIEKVPVVDGLSKVIGDYQIDGVPGQGAP
ISIDFSDTAGAKTGELLPTGEEVNKISTESCGEIEASLVDAGNPMVFVRAEDLGLRGNETPEEIDNNEEALKTLEEIRGK
AAVMMGIETDWKQAEQNNPAFPMVAFVSPASDSQQESGIDFNSRLMFMQVMHKTYAGTGTICTGSAAMIKGTIVNQVMSS
KKDQDEATIKIGHPAGFIEIEVRVDEDEQNGNWILNKAAINRTARRIMDGNCYIPKEG

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=373, Percent_Identity=34.5844504021448, Blast_Score=227, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 40870; Mature: 40870

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQEKVPTTIMRGGTSKAIFLKDKDLPTNQEERDNLILRIFGSPDPRQIDGLGGADPLTS
CCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCCCCC
KLAIIGPPTREDADIDYTFGQVSYTAAKIDYSGNCGNVSSAVGPFAIDKGYVQAEEPYTT
CEEEECCCCCCCCCCEEEECCEEEEEEEEEECCCCCCCCCCCCCEEECCCEEECCCCEEE
VRVHNTNTNKILIEKVPVVDGLSKVIGDYQIDGVPGQGAPISIDFSDTAGAKTGELLPTG
EEEECCCCCEEEEEECCCHHHHHHHHCCEEECCCCCCCCEEEEECCCCCCCCCCCCCCCC
EEVNKISTESCGEIEASLVDAGNPMVFVRAEDLGLRGNETPEEIDNNEEALKTLEEIRGK
HHHHHCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHCCC
AAVMMGIETDWKQAEQNNPAFPMVAFVSPASDSQQESGIDFNSRLMFMQVMHKTYAGTGT
EEEEEECCCCHHHHHCCCCCCEEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCC
ICTGSAAMIKGTIVNQVMSSKKDQDEATIKIGHPAGFIEIEVRVDEDEQNGNWILNKAAI
EEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCEEEEHHHH
NRTARRIMDGNCYIPKEG
HHHHHHHHCCCEEECCCC
>Mature Secondary Structure
MDQEKVPTTIMRGGTSKAIFLKDKDLPTNQEERDNLILRIFGSPDPRQIDGLGGADPLTS
CCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCCCCC
KLAIIGPPTREDADIDYTFGQVSYTAAKIDYSGNCGNVSSAVGPFAIDKGYVQAEEPYTT
CEEEECCCCCCCCCCEEEECCEEEEEEEEEECCCCCCCCCCCCCEEECCCEEECCCCEEE
VRVHNTNTNKILIEKVPVVDGLSKVIGDYQIDGVPGQGAPISIDFSDTAGAKTGELLPTG
EEEECCCCCEEEEEECCCHHHHHHHHCCEEECCCCCCCCEEEEECCCCCCCCCCCCCCCC
EEVNKISTESCGEIEASLVDAGNPMVFVRAEDLGLRGNETPEEIDNNEEALKTLEEIRGK
HHHHHCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHCCC
AAVMMGIETDWKQAEQNNPAFPMVAFVSPASDSQQESGIDFNSRLMFMQVMHKTYAGTGT
EEEEEECCCCHHHHHCCCCCCEEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCC
ICTGSAAMIKGTIVNQVMSSKKDQDEATIKIGHPAGFIEIEVRVDEDEQNGNWILNKAAI
EEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCEEEEHHHH
NRTARRIMDGNCYIPKEG
HHHHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA