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The map label for this gene is apr [H]

Identifier: 188586752

GI number: 188586752

Start: 2266654

End: 2267766

Strand: Direct

Name: apr [H]

Synonym: Nther_2143

Alternate gene names: 188586752

Gene position: 2266654-2267766 (Clockwise)

Preceding gene: 188586750

Following gene: 188586772

Centisome position: 71.6

GC content: 40.97

Gene sequence:

>1113_bases
ATGTTTAAAGTTGTCTGTATTTTGTTTGCGTTTTTAGTGATTCAAGGTGGTGCCTTCGCTCAGCCTGAAAGTGAGCACGA
TTTCAAAGAAGTTATCATTGGGTTTGAAGACAGACCAGGTCAAGCTGAATTAGAAATAATCGACGAAGCGAAAGGTGAAA
TTGATCAGGAATTTGAGATAATCAATGCAGTTTCCGCAAAGATTCCTGAACCAGCTGTTCAAGGTATGGAAAATGCAGAA
GGAATTGATTATGTTGAGCCCGATTACGAAGCCCAAACATTACAAACTATTCCTTGGGGTATAGAACAGATTAACGCTGA
TCAAGCTTGGGATGAAGCAACAGGTACTGGTATTGATGTAGCTATATTAGATACAGGAATTGACTACGGACACCAAGACC
TGGAAGTTGTAGATGGTGTTAACACAGTTGACGGCGGTGATTACATGGACTATAACGGACACGGTACCCATGTTGCAGGA
ACTGTAGCAGCATTAGATAATAATTCTGGTGTTGTAGGTGTAGCCCCTGATGCAAATCTATTTGCCGGAAAGGTCCTTGA
CGATGATGGTAGCGGTACTACTAGTGACATTGTAGCAGGTATCGAATGGGCAATTAACAATGATATGGATATAATTAACA
TGAGTCTGGGAATGTCTCAATATAGCACTGCTTTAGAAGATGTCTGTGATGAAGCTTATTATCAAGAAGATATACTTGTA
GTAGCTGCCGCAGGAAATGATGGTGAAGGCTGGTGGTGGGATCCTGACACCATCAATTATCCTGCTAATTACGATTCTGT
TATTGCAGTTGGAGCTACAGACCAAAACAATGATAGAGCTAACTTTTCCAGTGTAGGAGACAATCTAGAAATAATGGCTC
CTGGAGTTGATGTATTAAGTACTGTACCTGGTAATGATTATGATGAATATGATGGAACATCAATGGCATCTCCTCATATT
GCCGGAGTAGCGGCATTATTGATGGATCAGGGAGTATATACTTCTCCAGCAGAAGTACGAGATCGCATGAATGACACAGC
TGATGAACTGGGAGATCCATTTTACTACGGAAACGGCATTGTCAACACCACTGAAGCATTAGATATTCACTAA

Upstream 100 bases:

>100_bases
TTTTAAAACCAGTCCCCAAGTAAGTGTAGATAATTGTTAGGTGAGTGTAGTTAAATTGTTTAAGTGTAGCTTTGAAAAAT
TTTTAAGGAGGTCATTTACT

Downstream 100 bases:

>100_bases
ATCTGGATAAACTATCTAATACGTCAAGCTAATTATAACCTGCCGGGATATCCCGGCAGGTCCTTAAAAACAACAAAATT
AAAAATATACAAGAATTTAA

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MFKVVCILFAFLVIQGGAFAQPESEHDFKEVIIGFEDRPGQAELEIIDEAKGEIDQEFEIINAVSAKIPEPAVQGMENAE
GIDYVEPDYEAQTLQTIPWGIEQINADQAWDEATGTGIDVAILDTGIDYGHQDLEVVDGVNTVDGGDYMDYNGHGTHVAG
TVAALDNNSGVVGVAPDANLFAGKVLDDDGSGTTSDIVAGIEWAINNDMDIINMSLGMSQYSTALEDVCDEAYYQEDILV
VAAAGNDGEGWWWDPDTINYPANYDSVIAVGATDQNNDRANFSSVGDNLEIMAPGVDVLSTVPGNDYDEYDGTSMASPHI
AGVAALLMDQGVYTSPAEVRDRMNDTADELGDPFYYGNGIVNTTEALDIH

Sequences:

>Translated_370_residues
MFKVVCILFAFLVIQGGAFAQPESEHDFKEVIIGFEDRPGQAELEIIDEAKGEIDQEFEIINAVSAKIPEPAVQGMENAE
GIDYVEPDYEAQTLQTIPWGIEQINADQAWDEATGTGIDVAILDTGIDYGHQDLEVVDGVNTVDGGDYMDYNGHGTHVAG
TVAALDNNSGVVGVAPDANLFAGKVLDDDGSGTTSDIVAGIEWAINNDMDIINMSLGMSQYSTALEDVCDEAYYQEDILV
VAAAGNDGEGWWWDPDTINYPANYDSVIAVGATDQNNDRANFSSVGDNLEIMAPGVDVLSTVPGNDYDEYDGTSMASPHI
AGVAALLMDQGVYTSPAEVRDRMNDTADELGDPFYYGNGIVNTTEALDIH
>Mature_370_residues
MFKVVCILFAFLVIQGGAFAQPESEHDFKEVIIGFEDRPGQAELEIIDEAKGEIDQEFEIINAVSAKIPEPAVQGMENAE
GIDYVEPDYEAQTLQTIPWGIEQINADQAWDEATGTGIDVAILDTGIDYGHQDLEVVDGVNTVDGGDYMDYNGHGTHVAG
TVAALDNNSGVVGVAPDANLFAGKVLDDDGSGTTSDIVAGIEWAINNDMDIINMSLGMSQYSTALEDVCDEAYYQEDILV
VAAAGNDGEGWWWDPDTINYPANYDSVIAVGATDQNNDRANFSSVGDNLEIMAPGVDVLSTVPGNDYDEYDGTSMASPHI
AGVAALLMDQGVYTSPAEVRDRMNDTADELGDPFYYGNGIVNTTEALDIH

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=300, Percent_Identity=28, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI31317307, Length=288, Percent_Identity=28.8194444444444, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320775, Length=279, Percent_Identity=35.4838709677419, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6324576, Length=281, Percent_Identity=31.3167259786477, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319893, Length=232, Percent_Identity=36.2068965517241, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45550681, Length=288, Percent_Identity=29.5138888888889, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 39662; Mature: 39662

Theoretical pI: Translated: 3.41; Mature: 3.41

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKVVCILFAFLVIQGGAFAQPESEHDFKEVIIGFEDRPGQAELEIIDEAKGEIDQEFEI
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEECCCCCCCCCEEEEEHHHCCCHHHHHHH
INAVSAKIPEPAVQGMENAEGIDYVEPDYEAQTLQTIPWGIEQINADQAWDEATGTGIDV
HHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCHHHCCCCCCHHHCCCCCEEE
AILDTGIDYGHQDLEVVDGVNTVDGGDYMDYNGHGTHVAGTVAALDNNSGVVGVAPDANL
EEEECCCCCCCCCHHHHCCCCCCCCCCEECCCCCCCEEEEEEEEEECCCCEEEECCCCCE
FAGKVLDDDGSGTTSDIVAGIEWAINNDMDIINMSLGMSQYSTALEDVCDEAYYQEDILV
EECEEECCCCCCCHHHHHHHEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEE
VAAAGNDGEGWWWDPDTINYPANYDSVIAVGATDQNNDRANFSSVGDNLEIMAPGVDVLS
EEEECCCCCCCEECCCCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCEEEECCHHHHH
TVPGNDYDEYDGTSMASPHIAGVAALLMDQGVYTSPAEVRDRMNDTADELGDPFYYGNGI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHCCCEEECCCE
VNTTEALDIH
EECCCEEECC
>Mature Secondary Structure
MFKVVCILFAFLVIQGGAFAQPESEHDFKEVIIGFEDRPGQAELEIIDEAKGEIDQEFEI
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEECCCCCCCCCEEEEEHHHCCCHHHHHHH
INAVSAKIPEPAVQGMENAEGIDYVEPDYEAQTLQTIPWGIEQINADQAWDEATGTGIDV
HHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCHHHCCCCCCHHHCCCCCEEE
AILDTGIDYGHQDLEVVDGVNTVDGGDYMDYNGHGTHVAGTVAALDNNSGVVGVAPDANL
EEEECCCCCCCCCHHHHCCCCCCCCCCEECCCCCCCEEEEEEEEEECCCCEEEECCCCCE
FAGKVLDDDGSGTTSDIVAGIEWAINNDMDIINMSLGMSQYSTALEDVCDEAYYQEDILV
EECEEECCCCCCCHHHHHHHEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEE
VAAAGNDGEGWWWDPDTINYPANYDSVIAVGATDQNNDRANFSSVGDNLEIMAPGVDVLS
EEEECCCCCCCEECCCCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCEEEECCHHHHH
TVPGNDYDEYDGTSMASPHIAGVAALLMDQGVYTSPAEVRDRMNDTADELGDPFYYGNGI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHCCCEEECCCE
VNTTEALDIH
EECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3001653; 4967581; 8512925; 9425066 [H]