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The map label for this gene is pcrA [H]

Identifier: 188586746

GI number: 188586746

Start: 2256624

End: 2259218

Strand: Reverse

Name: pcrA [H]

Synonym: Nther_2137

Alternate gene names: 188586746

Gene position: 2259218-2256624 (Counterclockwise)

Preceding gene: 188586747

Following gene: 188586745

Centisome position: 71.37

GC content: 34.76

Gene sequence:

>2595_bases
TTGATTTCTCATCTAAACTATCATGTTGAAGAGCTGTTTCGAAAATTAAATGATTCACAAAAGCAGGCTGTAAATCAAAT
AGAAGGTTCTCTTTTGGTTACTGCACCTGTAGGTACAGGGAAAACAAGTGTTATAAGCCTTAGAGCTGCCAATGCTATTG
TAAATGGTTTTGATCCCAACAAGATTTTATGCCTTACTTTTACAAATCGTGCTGCTAGGGAAATGAAGGAAAGAATCATT
CAGGATTTAGGTCCAAGGGCACAAAATACAACTATAAAAACATTTCATGCTTTATGTGCTGAGATTATACGAATAGAATC
AGATATTTTGGGAATTCCAGCTGATTTTAATATATTTGATGAAGAAGATGCCAAGACAATTTTAAATGACGTTATAAAGA
CCAAAGACTTTCAAATTGCCAGTACTGATGCCAAAAGATTTATTAATTTTATGATGAATTATATTGCTCGAGTAAAAGCA
GCAAATGTAGATGTGGAAAAAATAAATGTTAATAACCCATTGGGAGAACAGGACAAGATGGATCAATTGTTTAGGGAAAG
ATTATCAGAAGCATATTTTAAAAACACGAGCAAAGATCAAGATTTCAAACACAAAGAGATATTTCGTGAATACAATCGGC
TTCTTTCCGAAAACCATGCCCTGGACTTTTCGGATCTAATATTTTATGTGGACAGGCTCTTCAGTGAGAGGACAGATGCT
AAAACACGATGGCAAGATAAATTTGAGTTTGTACAGGTTGATGAAGTACAAGATACTAGCTATCAGGAATATGGGATTAT
TTCTGAATTGAGTCAAAAACATAAAAATTTATCTTTATTTGGAGATATTCAGCAAACTGTCTATGAATGGAGAGGGTCTG
CACCCAATCAAGTGATGGAAAATTTTAAGTCTGAATTTTCTCCTGTAACAGAAATTAACTTAAATGTTAACTACCGTTCC
ACCCGAAATATTTTGAGGGCTTGTTACTGTGTAGCCAGTAAGTACAAAGGAGATTCTTTGGATATTTGGCCTGAAGCACA
AGTAGAAGGTGAAAAAACCGGGATTATTAAAGGTGCCCATCGGCAACAAGAAGTAGAGTTAATAGCAGATCAAATTTTTA
GATTGGTTCGGGAGCAAGGGTTTAAATTTCAAGATATTGCCTTATTGACCAGGACAAATCAAGTTAATCACTTGATTTCC
CAAGAACTAGCTAAAAACCAGATACCTCACTTTACTGTAGATAGTTATAAGTTCTTTAGAAGATCTGAAATAAAGGATGC
CATGGCGTACTTGAGATTATTGTTTAACAAACACGACAGTAATAGTTTGAGAAGAATTTTGCAAAAACCTGCCCGAGGTA
TTGGTGAAAAAACAATAGAAACACTCTTAAATATTCCATCTGATGTTGGCCTCAAGTTAGTAGATTTTGCCAATACATCA
ACTTTAAAATACAGAGATCCCTTTGCCCGATTAGTAAATAAATTTGAACAAAATAAATTAGTTGTTTTTGATGTTGAAAC
AACAGGTCTAGATACTACAAAGGATGAAATTATTGAATTAGCAGCTTTAAAGGCAGGGAAAGATGGTGTGGTTGATGAAT
TTCATGAATATTTACGAAACTCCAAGCCCGTTGGTGAATCATTCCAGGTTCACGGCATCTCAGATCAAGTCTTATTAGAG
CAAGGTAAACCTCCGGAGAAGTCTCTGGCTTCTTTTACTAATTTTATAGATGATTGTGACCTGGTAGGACATAATGTCAA
TTACGATATAACAATGGTAGAGAATCAGTGTAAAAAACTTTTAAATCAGGATTTGGAGCTACACGGCTATGATACTGTGG
ATATATCTCGTCGTTATTTTCCTAGATTGGAAAAGTATAACTTAGAAAGTCTCTGTGAAGATTTACAAATTTCTAGTAAA
CCGACTCATAGAGCTATGGATGATGTAGAAGCCACTTTTGAACTACTGCAGATCTTGTTAGAGAAAATCAAGCCCGGAAT
TGAAAAACGAAAAGAATATGTCAATAGTTATGGAGATAAATTTGCCAACCTGGCTATAGCCTTAAATTCCTGGCGCAGAA
AAACCTTGACTATGCGCCCTCCAGAACTACTTTCCCATGTCATCCAAGAATCCGGGTTGGCTGATTATTGGAAAAAAGAT
AAGCAACGAATTAATAACCTTAAAGAGTTAGTGTCAATTTTTGACTATTTTGATGACGAGACTCTATCTCCCAATATGTC
GTTAAAGAATATTTTAGAATATGTATCTTTGGGAAGTGACTCTGACAGGCATGTCTTGGACCAGGATAAAGTGGCATTAC
TAACTGTTCATCAGGCAAAAGGTTTAGAATTTGATGCGGTATTTATAGCTTCTGCCGATGACAATAATTTTCCCTACTTT
AGAAACAAAAAACTTGGCAATATCGATGAAGAACATAGATTGTTTTATGTAGCTATGACTAGGGCTAAACAAGCTTTATT
TATTAGCTGGGCTAGTAAAGATGAACATAGTTTTAATCAAGCCATCAGTCGATTTGCCAAATTAATACCTAAAAAATATC
TAAATTTTATAAAGAGTGATTACGATAAACACTAG

Upstream 100 bases:

>100_bases
GTTAAGGCCAAACATCCAGGTTTTTTTATACTTTCTAGGCGAACAGGAGTTTGCTATAATTGAGTAAGTAAGGATGTTTA
ATCCAGGAGGGGTTGTGGTT

Downstream 100 bases:

>100_bases
CAGAAAGCCCACACCACTACTTTAGCTGTGGGAAAATATAAAACTCCTAAATGTTTAAATTCCAAAGTATGAAGGCCAAT
GGTTTAGAATATTATTAATC

Product: DNA polymerase III, epsilon subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 864; Mature: 864

Protein sequence:

>864_residues
MISHLNYHVEELFRKLNDSQKQAVNQIEGSLLVTAPVGTGKTSVISLRAANAIVNGFDPNKILCLTFTNRAAREMKERII
QDLGPRAQNTTIKTFHALCAEIIRIESDILGIPADFNIFDEEDAKTILNDVIKTKDFQIASTDAKRFINFMMNYIARVKA
ANVDVEKINVNNPLGEQDKMDQLFRERLSEAYFKNTSKDQDFKHKEIFREYNRLLSENHALDFSDLIFYVDRLFSERTDA
KTRWQDKFEFVQVDEVQDTSYQEYGIISELSQKHKNLSLFGDIQQTVYEWRGSAPNQVMENFKSEFSPVTEINLNVNYRS
TRNILRACYCVASKYKGDSLDIWPEAQVEGEKTGIIKGAHRQQEVELIADQIFRLVREQGFKFQDIALLTRTNQVNHLIS
QELAKNQIPHFTVDSYKFFRRSEIKDAMAYLRLLFNKHDSNSLRRILQKPARGIGEKTIETLLNIPSDVGLKLVDFANTS
TLKYRDPFARLVNKFEQNKLVVFDVETTGLDTTKDEIIELAALKAGKDGVVDEFHEYLRNSKPVGESFQVHGISDQVLLE
QGKPPEKSLASFTNFIDDCDLVGHNVNYDITMVENQCKKLLNQDLELHGYDTVDISRRYFPRLEKYNLESLCEDLQISSK
PTHRAMDDVEATFELLQILLEKIKPGIEKRKEYVNSYGDKFANLAIALNSWRRKTLTMRPPELLSHVIQESGLADYWKKD
KQRINNLKELVSIFDYFDDETLSPNMSLKNILEYVSLGSDSDRHVLDQDKVALLTVHQAKGLEFDAVFIASADDNNFPYF
RNKKLGNIDEEHRLFYVAMTRAKQALFISWASKDEHSFNQAISRFAKLIPKKYLNFIKSDYDKH

Sequences:

>Translated_864_residues
MISHLNYHVEELFRKLNDSQKQAVNQIEGSLLVTAPVGTGKTSVISLRAANAIVNGFDPNKILCLTFTNRAAREMKERII
QDLGPRAQNTTIKTFHALCAEIIRIESDILGIPADFNIFDEEDAKTILNDVIKTKDFQIASTDAKRFINFMMNYIARVKA
ANVDVEKINVNNPLGEQDKMDQLFRERLSEAYFKNTSKDQDFKHKEIFREYNRLLSENHALDFSDLIFYVDRLFSERTDA
KTRWQDKFEFVQVDEVQDTSYQEYGIISELSQKHKNLSLFGDIQQTVYEWRGSAPNQVMENFKSEFSPVTEINLNVNYRS
TRNILRACYCVASKYKGDSLDIWPEAQVEGEKTGIIKGAHRQQEVELIADQIFRLVREQGFKFQDIALLTRTNQVNHLIS
QELAKNQIPHFTVDSYKFFRRSEIKDAMAYLRLLFNKHDSNSLRRILQKPARGIGEKTIETLLNIPSDVGLKLVDFANTS
TLKYRDPFARLVNKFEQNKLVVFDVETTGLDTTKDEIIELAALKAGKDGVVDEFHEYLRNSKPVGESFQVHGISDQVLLE
QGKPPEKSLASFTNFIDDCDLVGHNVNYDITMVENQCKKLLNQDLELHGYDTVDISRRYFPRLEKYNLESLCEDLQISSK
PTHRAMDDVEATFELLQILLEKIKPGIEKRKEYVNSYGDKFANLAIALNSWRRKTLTMRPPELLSHVIQESGLADYWKKD
KQRINNLKELVSIFDYFDDETLSPNMSLKNILEYVSLGSDSDRHVLDQDKVALLTVHQAKGLEFDAVFIASADDNNFPYF
RNKKLGNIDEEHRLFYVAMTRAKQALFISWASKDEHSFNQAISRFAKLIPKKYLNFIKSDYDKH
>Mature_864_residues
MISHLNYHVEELFRKLNDSQKQAVNQIEGSLLVTAPVGTGKTSVISLRAANAIVNGFDPNKILCLTFTNRAAREMKERII
QDLGPRAQNTTIKTFHALCAEIIRIESDILGIPADFNIFDEEDAKTILNDVIKTKDFQIASTDAKRFINFMMNYIARVKA
ANVDVEKINVNNPLGEQDKMDQLFRERLSEAYFKNTSKDQDFKHKEIFREYNRLLSENHALDFSDLIFYVDRLFSERTDA
KTRWQDKFEFVQVDEVQDTSYQEYGIISELSQKHKNLSLFGDIQQTVYEWRGSAPNQVMENFKSEFSPVTEINLNVNYRS
TRNILRACYCVASKYKGDSLDIWPEAQVEGEKTGIIKGAHRQQEVELIADQIFRLVREQGFKFQDIALLTRTNQVNHLIS
QELAKNQIPHFTVDSYKFFRRSEIKDAMAYLRLLFNKHDSNSLRRILQKPARGIGEKTIETLLNIPSDVGLKLVDFANTS
TLKYRDPFARLVNKFEQNKLVVFDVETTGLDTTKDEIIELAALKAGKDGVVDEFHEYLRNSKPVGESFQVHGISDQVLLE
QGKPPEKSLASFTNFIDDCDLVGHNVNYDITMVENQCKKLLNQDLELHGYDTVDISRRYFPRLEKYNLESLCEDLQISSK
PTHRAMDDVEATFELLQILLEKIKPGIEKRKEYVNSYGDKFANLAIALNSWRRKTLTMRPPELLSHVIQESGLADYWKKD
KQRINNLKELVSIFDYFDDETLSPNMSLKNILEYVSLGSDSDRHVLDQDKVALLTVHQAKGLEFDAVFIASADDNNFPYF
RNKKLGNIDEEHRLFYVAMTRAKQALFISWASKDEHSFNQAISRFAKLIPKKYLNFIKSDYDKH

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=451, Percent_Identity=30.1552106430155, Blast_Score=189, Evalue=8e-49,
Organism=Escherichia coli, GI2367296, Length=470, Percent_Identity=27.2340425531915, Blast_Score=186, Evalue=7e-48,
Organism=Escherichia coli, GI1787196, Length=320, Percent_Identity=27.5, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322369, Length=545, Percent_Identity=25.6880733944954, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 99806; Mature: 99806

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISHLNYHVEELFRKLNDSQKQAVNQIEGSLLVTAPVGTGKTSVISLRAANAIVNGFDPN
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCCC
KILCLTFTNRAAREMKERIIQDLGPRAQNTTIKTFHALCAEIIRIESDILGIPADFNIFD
EEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EEDAKTILNDVIKTKDFQIASTDAKRFINFMMNYIARVKAANVDVEKINVNNPLGEQDKM
CHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
DQLFRERLSEAYFKNTSKDQDFKHKEIFREYNRLLSENHALDFSDLIFYVDRLFSERTDA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
KTRWQDKFEFVQVDEVQDTSYQEYGIISELSQKHKNLSLFGDIQQTVYEWRGSAPNQVME
HHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
NFKSEFSPVTEINLNVNYRSTRNILRACYCVASKYKGDSLDIWPEAQVEGEKTGIIKGAH
HHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCC
RQQEVELIADQIFRLVREQGFKFQDIALLTRTNQVNHLISQELAKNQIPHFTVDSYKFFR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHH
RSEIKDAMAYLRLLFNKHDSNSLRRILQKPARGIGEKTIETLLNIPSDVGLKLVDFANTS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCC
TLKYRDPFARLVNKFEQNKLVVFDVETTGLDTTKDEIIELAALKAGKDGVVDEFHEYLRN
CEEECCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHC
SKPVGESFQVHGISDQVLLEQGKPPEKSLASFTNFIDDCDLVGHNVNYDITMVENQCKKL
CCCCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
LNQDLELHGYDTVDISRRYFPRLEKYNLESLCEDLQISSKPTHRAMDDVEATFELLQILL
HCCCCCCCCCCCHHHHHHHCCHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
EKIKPGIEKRKEYVNSYGDKFANLAIALNSWRRKTLTMRPPELLSHVIQESGLADYWKKD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHH
KQRINNLKELVSIFDYFDDETLSPNMSLKNILEYVSLGSDSDRHVLDQDKVALLTVHQAK
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCC
GLEFDAVFIASADDNNFPYFRNKKLGNIDEEHRLFYVAMTRAKQALFISWASKDEHSFNQ
CCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHEEEEEHHHCCCEEEEEECCCCHHHHHH
AISRFAKLIPKKYLNFIKSDYDKH
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISHLNYHVEELFRKLNDSQKQAVNQIEGSLLVTAPVGTGKTSVISLRAANAIVNGFDPN
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCCC
KILCLTFTNRAAREMKERIIQDLGPRAQNTTIKTFHALCAEIIRIESDILGIPADFNIFD
EEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EEDAKTILNDVIKTKDFQIASTDAKRFINFMMNYIARVKAANVDVEKINVNNPLGEQDKM
CHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
DQLFRERLSEAYFKNTSKDQDFKHKEIFREYNRLLSENHALDFSDLIFYVDRLFSERTDA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
KTRWQDKFEFVQVDEVQDTSYQEYGIISELSQKHKNLSLFGDIQQTVYEWRGSAPNQVME
HHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
NFKSEFSPVTEINLNVNYRSTRNILRACYCVASKYKGDSLDIWPEAQVEGEKTGIIKGAH
HHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCC
RQQEVELIADQIFRLVREQGFKFQDIALLTRTNQVNHLISQELAKNQIPHFTVDSYKFFR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHH
RSEIKDAMAYLRLLFNKHDSNSLRRILQKPARGIGEKTIETLLNIPSDVGLKLVDFANTS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCC
TLKYRDPFARLVNKFEQNKLVVFDVETTGLDTTKDEIIELAALKAGKDGVVDEFHEYLRN
CEEECCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHC
SKPVGESFQVHGISDQVLLEQGKPPEKSLASFTNFIDDCDLVGHNVNYDITMVENQCKKL
CCCCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
LNQDLELHGYDTVDISRRYFPRLEKYNLESLCEDLQISSKPTHRAMDDVEATFELLQILL
HCCCCCCCCCCCHHHHHHHCCHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
EKIKPGIEKRKEYVNSYGDKFANLAIALNSWRRKTLTMRPPELLSHVIQESGLADYWKKD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHH
KQRINNLKELVSIFDYFDDETLSPNMSLKNILEYVSLGSDSDRHVLDQDKVALLTVHQAK
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCC
GLEFDAVFIASADDNNFPYFRNKKLGNIDEEHRLFYVAMTRAKQALFISWASKDEHSFNQ
CCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHEEEEEHHHCCCEEEEEECCCCHHHHHH
AISRFAKLIPKKYLNFIKSDYDKH
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]