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The map label for this gene is nodB [H]

Identifier: 188586674

GI number: 188586674

Start: 2182063

End: 2183169

Strand: Reverse

Name: nodB [H]

Synonym: Nther_2064

Alternate gene names: 188586674

Gene position: 2183169-2182063 (Counterclockwise)

Preceding gene: 188586675

Following gene: 188586673

Centisome position: 68.97

GC content: 36.13

Gene sequence:

>1107_bases
ATGAAAGGGCACAACCTAATTTTACTTGTCATAATAGCTTTATTGCTTTTACCTACTACAATTTTTGCAGAAGAAACACA
ATTAGAAGTTCAAGAAAAGGAAATTGAAGAAGCTGAGGAAGAGTATCTGCAAGTTTACAGGGTTGAATCAGGGGATAATT
TGTATTCACTGGCTAGTGAATTTGAAACCAGTATTGACGAATTAATCTCACTAAATCCTATCGAGAAGCCAGATCATCTG
TTAGAAGGTCAAGTGATGGTGGTTCCAGGCAGAGTGACAGAAGAATATACCATTGAGTCAGGGGATACATTGTCTCAAAT
AGCTGAGGATTATGAAGTTGATATGGACATGCTTGCGGAGTATAATAATTTAAAAGACGAAGACATATTAATTTCCGGAC
AAACCTTAGAAATCCCTTTAACTCACCCTTCTTATGAAATGAATGATGGTTATAGTAACTGGTATCCCCAAAATAACTGG
GAAAACTTGTATAACTTCGGTGAAGAAGCGGATAAACAGATTGCACTAACCTTCGATGATGGTCCAGACGATTATTATAC
AGATGAAGTTTTGGATATTTTAGATGAATACCAAATCCCCAGTACCTTTTATTTTGTTGGGAACCAAATTGAAAATTATC
CTGAAGTAGTAGAGCGCACTGACTCTGAGGGACATGAAATAGGCAACCATTCTTGGGACCATTCAAACTTCTTAGATTTA
GAATCAGAAGAAATTGAAGAACAAGTTCTTAAAACAGAAGAAGCTATCAAAGAAATTACTGGCAACCGGACAGCAACTTT
CCGCCCACCTTATGGAGCCTTAGATACTCAAGGGATAGCCCAATTGGCAGAAATGGATTACAACGTGATTAATTGGTCAG
TAGATTCTTGGGACTGGTTAGACAAGCATCCAGATTTATTTCATATTAATGTATTACCAAATGTAGATAGTGGTTCGATT
ATTTTAATGCATTCATATTCAAGCGGAGAAGATGATAATCGAGACCATTTGTTAGAAGCATTGCCGGAGCTAATAGAAAC
TCTAAAATCACAGGGATATGAATTTGTGACAGTAAGTGAATTATTAAATGTTAATTCCTATAAGTAA

Upstream 100 bases:

>100_bases
CGGTCGAACTAGTTGTAACCCTACCCTTTCCGTGCTATAGTATTGATAGCAATATTGAAAGTAATATATATAACAGTATA
TAACGGAAAGGGGTAAATAA

Downstream 100 bases:

>100_bases
GTTAGATTAACAAGTTTAAATCAATTGAAGTTGAAGAGACTGTCCTAGTATCTTACGAGTTAACCAGGACAGTCTTTTTT
GTTAACTAAAATAGTAAAAT

Product: polysaccharide deacetylase

Products: NA

Alternate protein names: Nodulation protein B [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKGHNLILLVIIALLLLPTTIFAEETQLEVQEKEIEEAEEEYLQVYRVESGDNLYSLASEFETSIDELISLNPIEKPDHL
LEGQVMVVPGRVTEEYTIESGDTLSQIAEDYEVDMDMLAEYNNLKDEDILISGQTLEIPLTHPSYEMNDGYSNWYPQNNW
ENLYNFGEEADKQIALTFDDGPDDYYTDEVLDILDEYQIPSTFYFVGNQIENYPEVVERTDSEGHEIGNHSWDHSNFLDL
ESEEIEEQVLKTEEAIKEITGNRTATFRPPYGALDTQGIAQLAEMDYNVINWSVDSWDWLDKHPDLFHINVLPNVDSGSI
ILMHSYSSGEDDNRDHLLEALPELIETLKSQGYEFVTVSELLNVNSYK

Sequences:

>Translated_368_residues
MKGHNLILLVIIALLLLPTTIFAEETQLEVQEKEIEEAEEEYLQVYRVESGDNLYSLASEFETSIDELISLNPIEKPDHL
LEGQVMVVPGRVTEEYTIESGDTLSQIAEDYEVDMDMLAEYNNLKDEDILISGQTLEIPLTHPSYEMNDGYSNWYPQNNW
ENLYNFGEEADKQIALTFDDGPDDYYTDEVLDILDEYQIPSTFYFVGNQIENYPEVVERTDSEGHEIGNHSWDHSNFLDL
ESEEIEEQVLKTEEAIKEITGNRTATFRPPYGALDTQGIAQLAEMDYNVINWSVDSWDWLDKHPDLFHINVLPNVDSGSI
ILMHSYSSGEDDNRDHLLEALPELIETLKSQGYEFVTVSELLNVNSYK
>Mature_368_residues
MKGHNLILLVIIALLLLPTTIFAEETQLEVQEKEIEEAEEEYLQVYRVESGDNLYSLASEFETSIDELISLNPIEKPDHL
LEGQVMVVPGRVTEEYTIESGDTLSQIAEDYEVDMDMLAEYNNLKDEDILISGQTLEIPLTHPSYEMNDGYSNWYPQNNW
ENLYNFGEEADKQIALTFDDGPDDYYTDEVLDILDEYQIPSTFYFVGNQIENYPEVVERTDSEGHEIGNHSWDHSNFLDL
ESEEIEEQVLKTEEAIKEITGNRTATFRPPYGALDTQGIAQLAEMDYNVINWSVDSWDWLDKHPDLFHINVLPNVDSGSI
ILMHSYSSGEDDNRDHLLEALPELIETLKSQGYEFVTVSELLNVNSYK

Specific function: Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323339, Length=128, Percent_Identity=34.375, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323338, Length=127, Percent_Identity=33.8582677165354, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 42276; Mature: 42276

Theoretical pI: Translated: 3.70; Mature: 3.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGHNLILLVIIALLLLPTTIFAEETQLEVQEKEIEEAEEEYLQVYRVESGDNLYSLASE
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHH
FETSIDELISLNPIEKPDHLLEGQVMVVPGRVTEEYTIESGDTLSQIAEDYEVDMDMLAE
HHHHHHHHHCCCCCCCCCHHHCCEEEEECCCCCCCEEECCCCHHHHHHHHHCCCHHHHHH
YNNLKDEDILISGQTLEIPLTHPSYEMNDGYSNWYPQNNWENLYNFGEEADKQIALTFDD
HCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEECC
GPDDYYTDEVLDILDEYQIPSTFYFVGNQIENYPEVVERTDSEGHEIGNHSWDHSNFLDL
CCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
ESEEIEEQVLKTEEAIKEITGNRTATFRPPYGALDTQGIAQLAEMDYNVINWSVDSWDWL
CHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCHHH
DKHPDLFHINVLPNVDSGSIILMHSYSSGEDDNRDHLLEALPELIETLKSQGYEFVTVSE
CCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHH
LLNVNSYK
HHCCCCCC
>Mature Secondary Structure
MKGHNLILLVIIALLLLPTTIFAEETQLEVQEKEIEEAEEEYLQVYRVESGDNLYSLASE
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHH
FETSIDELISLNPIEKPDHLLEGQVMVVPGRVTEEYTIESGDTLSQIAEDYEVDMDMLAE
HHHHHHHHHCCCCCCCCCHHHCCEEEEECCCCCCCEEECCCCHHHHHHHHHCCCHHHHHH
YNNLKDEDILISGQTLEIPLTHPSYEMNDGYSNWYPQNNWENLYNFGEEADKQIALTFDD
HCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEECC
GPDDYYTDEVLDILDEYQIPSTFYFVGNQIENYPEVVERTDSEGHEIGNHSWDHSNFLDL
CCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
ESEEIEEQVLKTEEAIKEITGNRTATFRPPYGALDTQGIAQLAEMDYNVINWSVDSWDWL
CHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCHHH
DKHPDLFHINVLPNVDSGSIILMHSYSSGEDDNRDHLLEALPELIETLKSQGYEFVTVSE
CCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHH
LLNVNSYK
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3960737 [H]