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The map label for this gene is tnaA [H]

Identifier: 188586632

GI number: 188586632

Start: 2133139

End: 2134527

Strand: Reverse

Name: tnaA [H]

Synonym: Nther_2022

Alternate gene names: 188586632

Gene position: 2134527-2133139 (Counterclockwise)

Preceding gene: 188586633

Following gene: 188586631

Centisome position: 67.43

GC content: 36.79

Gene sequence:

>1389_bases
ATGGGTAATATTATTGATGAAATGATGGCGGCTGAACCGTTTAGGATTAAAATGGTTGAGCCGATTAAGACAACTACCAA
GGAAGAACGCCAGAGAAAAATTAAAGAAGCGGGTTATAATGTTTTTAATTTGGAATCTAAGGATGTTTATATCGATTTAT
TAACAGATAGTGGAACAAGTGCCATGAGTGATAATCAATGGGCTGGTATGATGTTAGGAGATGAGGCATATGCTGGTAGT
AAAAACTTCTATAATCTCAAGGATGCCATCAAAGAAGTGATGGGTTATGACTACTTTTTACCTACACATCAAGGGAGAGC
CGCAGAAAATGTACTTTTTCAATTATACGTGGAAGAAGGAAATTATGTACCTAATAATATGCATTTTGATACCACAAAGG
CACATGTTCAAGATAAACGTGGGCGACCTGTCAATTTAGTTATAGATGAAGCTTATGATGCCAAGAAAAATGTGCCATTT
AAAGGAAATATGGATATTGATAAACTAGATAATTTCTTAAAAGAGCACAGTGATAACGTTCCCATTGTATTATTGACAGT
AACGTGTAATAGCGGTGGTGGTCAACCCGTTTCTATGGAAAATATCAAAGAAGTGTCAGAAATTTGTAAGAAATATAATA
AACCTTTCTATTTAGATGCTTGTCGCTTCGCAGAAAATGCTTATTTTATTAAACTAAGAGAGCCAGGCTATGCCGATAAA
TCCATTCCAGAGATAACCCGGGAGATGTTCTCTTATGCAGATGGTTGCACCATGAGTTCCAAAAAAGATGCTTTAGTTAA
TATTGGTGGTTTTTTAGCAATGGAAGATGAGAAACTACTTGATAAAGCAAAAGTTTTAGGAGTACTATATGAAGGGTTCC
CTACTTACGGGGGTATGGCTGGCAGAGATATGGAAGCCATGGCCCGTGGATTACTTGAAGTTGTGGAAGAAGAATATTTA
CAATACAGAATAAACCAGGTTAATTATTTAGGTGAAAAGTTGAAGGAAAAGAAAGTACCTATATTGGAACCCGTTGGTGG
ACACGCTGTATATCTAGATGCTGGTCGTTTCTTACCAAATATTCCAAAAGACCAATTTCCTGGTCAAGCTTTAACAGTAG
CACTTTACGAAGAGGGCGGTATTAGAGGTGTTGAAATAGGAACTGTCTTAAGTGGAAGAGATCCTGAAACCGGAGACCAT
GATTATCCCGAATTAGAACTGGTTCGTTTAACTATACCCAGAAGAGTATATACTTATCGACATATGGATGTTGTGGCAGA
GGCTGCTAAAATAATTCATGATAAGCGAGATGAAATTGGTGGCTATAAATTTACTTATGAACCTGAGATCTTAAGGCATT
TTACTGCCAGATTTGCTCCTGTAAAATAA

Upstream 100 bases:

>100_bases
GGCATGATTCGGAATTACTTGGGGAAGTCATTATTAAAGAAGTGATCAATAAGAAAAAAGAAGTGATTCGAAAAAAATTA
TTTGCCAGGAGGGATTAAAG

Downstream 100 bases:

>100_bases
ATTAAACACAGTTAGGTGATAAAAATGTCCTTTGCTAAATCATGCAAAAATGAACTTTCAAGAATTGAAATTAATAGAGA
ATGCTGCGAAAGAGCTGAAC

Product: tryptophanase

Products: NA

Alternate protein names: L-tryptophan indole-lyase; TNase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MGNIIDEMMAAEPFRIKMVEPIKTTTKEERQRKIKEAGYNVFNLESKDVYIDLLTDSGTSAMSDNQWAGMMLGDEAYAGS
KNFYNLKDAIKEVMGYDYFLPTHQGRAAENVLFQLYVEEGNYVPNNMHFDTTKAHVQDKRGRPVNLVIDEAYDAKKNVPF
KGNMDIDKLDNFLKEHSDNVPIVLLTVTCNSGGGQPVSMENIKEVSEICKKYNKPFYLDACRFAENAYFIKLREPGYADK
SIPEITREMFSYADGCTMSSKKDALVNIGGFLAMEDEKLLDKAKVLGVLYEGFPTYGGMAGRDMEAMARGLLEVVEEEYL
QYRINQVNYLGEKLKEKKVPILEPVGGHAVYLDAGRFLPNIPKDQFPGQALTVALYEEGGIRGVEIGTVLSGRDPETGDH
DYPELELVRLTIPRRVYTYRHMDVVAEAAKIIHDKRDEIGGYKFTYEPEILRHFTARFAPVK

Sequences:

>Translated_462_residues
MGNIIDEMMAAEPFRIKMVEPIKTTTKEERQRKIKEAGYNVFNLESKDVYIDLLTDSGTSAMSDNQWAGMMLGDEAYAGS
KNFYNLKDAIKEVMGYDYFLPTHQGRAAENVLFQLYVEEGNYVPNNMHFDTTKAHVQDKRGRPVNLVIDEAYDAKKNVPF
KGNMDIDKLDNFLKEHSDNVPIVLLTVTCNSGGGQPVSMENIKEVSEICKKYNKPFYLDACRFAENAYFIKLREPGYADK
SIPEITREMFSYADGCTMSSKKDALVNIGGFLAMEDEKLLDKAKVLGVLYEGFPTYGGMAGRDMEAMARGLLEVVEEEYL
QYRINQVNYLGEKLKEKKVPILEPVGGHAVYLDAGRFLPNIPKDQFPGQALTVALYEEGGIRGVEIGTVLSGRDPETGDH
DYPELELVRLTIPRRVYTYRHMDVVAEAAKIIHDKRDEIGGYKFTYEPEILRHFTARFAPVK
>Mature_461_residues
GNIIDEMMAAEPFRIKMVEPIKTTTKEERQRKIKEAGYNVFNLESKDVYIDLLTDSGTSAMSDNQWAGMMLGDEAYAGSK
NFYNLKDAIKEVMGYDYFLPTHQGRAAENVLFQLYVEEGNYVPNNMHFDTTKAHVQDKRGRPVNLVIDEAYDAKKNVPFK
GNMDIDKLDNFLKEHSDNVPIVLLTVTCNSGGGQPVSMENIKEVSEICKKYNKPFYLDACRFAENAYFIKLREPGYADKS
IPEITREMFSYADGCTMSSKKDALVNIGGFLAMEDEKLLDKAKVLGVLYEGFPTYGGMAGRDMEAMARGLLEVVEEEYLQ
YRINQVNYLGEKLKEKKVPILEPVGGHAVYLDAGRFLPNIPKDQFPGQALTVALYEEGGIRGVEIGTVLSGRDPETGDHD
YPELELVRLTIPRRVYTYRHMDVVAEAAKIIHDKRDEIGGYKFTYEPEILRHFTARFAPVK

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family [H]

Homologues:

Organism=Escherichia coli, GI87082323, Length=464, Percent_Identity=47.198275862069, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166
- InterPro:   IPR018176 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: =4.1.99.1 [H]

Molecular weight: Translated: 52320; Mature: 52189

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNIIDEMMAAEPFRIKMVEPIKTTTKEERQRKIKEAGYNVFNLESKDVYIDLLTDSGTS
CCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCC
AMSDNQWAGMMLGDEAYAGSKNFYNLKDAIKEVMGYDYFLPTHQGRAAENVLFQLYVEEG
CCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHCEEEEEEEECC
NYVPNNMHFDTTKAHVQDKRGRPVNLVIDEAYDAKKNVPFKGNMDIDKLDNFLKEHSDNV
CCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
PIVLLTVTCNSGGGQPVSMENIKEVSEICKKYNKPFYLDACRFAENAYFIKLREPGYADK
CEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCEEEEEECCCCCCCC
SIPEITREMFSYADGCTMSSKKDALVNIGGFLAMEDEKLLDKAKVLGVLYEGFPTYGGMA
CHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCC
GRDMEAMARGLLEVVEEEYLQYRINQVNYLGEKLKEKKVPILEPVGGHAVYLDAGRFLPN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEECCCCCCC
IPKDQFPGQALTVALYEEGGIRGVEIGTVLSGRDPETGDHDYPELELVRLTIPRRVYTYR
CCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCHHHHHH
HMDVVAEAAKIIHDKRDEIGGYKFTYEPEILRHFTARFAPVK
HHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GNIIDEMMAAEPFRIKMVEPIKTTTKEERQRKIKEAGYNVFNLESKDVYIDLLTDSGTS
CCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCC
AMSDNQWAGMMLGDEAYAGSKNFYNLKDAIKEVMGYDYFLPTHQGRAAENVLFQLYVEEG
CCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHCEEEEEEEECC
NYVPNNMHFDTTKAHVQDKRGRPVNLVIDEAYDAKKNVPFKGNMDIDKLDNFLKEHSDNV
CCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
PIVLLTVTCNSGGGQPVSMENIKEVSEICKKYNKPFYLDACRFAENAYFIKLREPGYADK
CEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCEEEEEECCCCCCCC
SIPEITREMFSYADGCTMSSKKDALVNIGGFLAMEDEKLLDKAKVLGVLYEGFPTYGGMA
CHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCC
GRDMEAMARGLLEVVEEEYLQYRINQVNYLGEKLKEKKVPILEPVGGHAVYLDAGRFLPN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEECCCCCCC
IPKDQFPGQALTVALYEEGGIRGVEIGTVLSGRDPETGDHDYPELELVRLTIPRRVYTYR
CCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCHHHHHH
HMDVVAEAAKIIHDKRDEIGGYKFTYEPEILRHFTARFAPVK
HHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]