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The map label for this gene is eno

Identifier: 188586625

GI number: 188586625

Start: 2124443

End: 2125726

Strand: Reverse

Name: eno

Synonym: Nther_2015

Alternate gene names: 188586625

Gene position: 2125726-2124443 (Counterclockwise)

Preceding gene: 188586626

Following gene: 188586624

Centisome position: 67.15

GC content: 38.86

Gene sequence:

>1284_bases
ATGAGCATGATAGAAGAAATTTACGCTAGGGAAATATTTGATTCACGTGGTAATCCAACTGTAGAAGTTGAACTTTATAC
TGAATCAGGTGCTTATGGTTTCGCCAGGGTACCTTCTGGCGCTTCCACAGGTGTACATGAAGCATTAGAATTAAGAGACG
GTGAAGATAGATTTGGGGGTAAAGGTGTTAGAAAGGCGTGTAGCAAAGTTAATGAGGAAATTGGTCCCAATTTGGTTGGG
ATGGATGTTACTTTTCAATCTGCCATCGATAGAGCGCTACTAGAACTAGACGGAACAGATAATAAGGAAAATCTAGGAGC
TAATGCCATGTTGGGAGTTTCTCTAGCAGCTGCTAGAGCTTCGGCTGAATTTTTAGGGCTTCCTTTATATCAAGTGTTGG
GAGGAGTAAGTAGTAGTACCTTACCAATACCCCAGATGAATATTCTTAATGGCGGGGAACACGCAGATAATAATGTAGAT
ATCCAAGAGTTTATGATTATGCCTATTAGGGCCAATAACTTCCAACACGCTATGAGAATGGGAGTTGAAATTTTCCATGC
CTTGAAAAATGTTTTAAAAGATGAAGGATTATCAACTTCAGTGGGAGATGAAGGTGGTTTTGCTCCTGATCTCAAATCCA
ATAAAGAAGCATTAGAATATATTATTACTGCTATTGAAAAAGTAGGTTACAAGCCTGGTGAACAAGTTATGTTAGCTATA
GATGCTGCAGCTTCAGAGCTGTACCAAGGTGATAAATATCATCTTGAGGGAGAAGGTAAAAGCTTAACCGCAGATGAAAT
GATTGATTTGTATCAGGACCTAGTAGAAAACTATCCAATTATTTCCATCGAAGACGGGTTGTCAGAAGATGATTGGGAAG
GTTGGAAAAAAATGACTGAAAAGTTCCAGGGAAAAATACAGCTAGTAGGGGACGATTTGTTTGTTACAAACACTGACAGA
TTGACTCGAGGAATCAAAGAAGATATAGCTAATAGTATTTTGATTAAGCTAAATCAGATTGGAACAGTTACAGAAACCTT
AGAGGCAATTGAACTGGCTAAAAAGAATGGATATACATCTGTGATTTCTCATCGCTCCGGAGAAACAGATGATCCCTTTA
TTGCGGATCTAGCTGTGGCAACCAATGCAGGTCAGATTAAAACCGGAGCTCCATCTAGAATGGACCGGGTAGCCAAGTAC
AATCAATTAATCAGAATTTCAGAAGAGCTTTATGGAGTTTCAAGATATCCAGGAATGGGAAGTTTTTATAATCTAGAAGT
TTAG

Upstream 100 bases:

>100_bases
ACCAACAGTATTAGAGTTAATGGGACTACCATTGCCCCAAGAAATGTCGGGTAAATCATTAATAGATCATTAAAATAAAT
TCAAACTGGAGGGATATATA

Downstream 100 bases:

>100_bases
TCTTTCACTGTTGCTTTTTTTATTCCCCCTATGATAAAATAACACGGGATAAGGGGGTGTAATTAAAATCATGTTGTATT
CAGTTTTAGTAGGTTTACAC

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSMIEEIYAREIFDSRGNPTVEVELYTESGAYGFARVPSGASTGVHEALELRDGEDRFGGKGVRKACSKVNEEIGPNLVG
MDVTFQSAIDRALLELDGTDNKENLGANAMLGVSLAAARASAEFLGLPLYQVLGGVSSSTLPIPQMNILNGGEHADNNVD
IQEFMIMPIRANNFQHAMRMGVEIFHALKNVLKDEGLSTSVGDEGGFAPDLKSNKEALEYIITAIEKVGYKPGEQVMLAI
DAAASELYQGDKYHLEGEGKSLTADEMIDLYQDLVENYPIISIEDGLSEDDWEGWKKMTEKFQGKIQLVGDDLFVTNTDR
LTRGIKEDIANSILIKLNQIGTVTETLEAIELAKKNGYTSVISHRSGETDDPFIADLAVATNAGQIKTGAPSRMDRVAKY
NQLIRISEELYGVSRYPGMGSFYNLEV

Sequences:

>Translated_427_residues
MSMIEEIYAREIFDSRGNPTVEVELYTESGAYGFARVPSGASTGVHEALELRDGEDRFGGKGVRKACSKVNEEIGPNLVG
MDVTFQSAIDRALLELDGTDNKENLGANAMLGVSLAAARASAEFLGLPLYQVLGGVSSSTLPIPQMNILNGGEHADNNVD
IQEFMIMPIRANNFQHAMRMGVEIFHALKNVLKDEGLSTSVGDEGGFAPDLKSNKEALEYIITAIEKVGYKPGEQVMLAI
DAAASELYQGDKYHLEGEGKSLTADEMIDLYQDLVENYPIISIEDGLSEDDWEGWKKMTEKFQGKIQLVGDDLFVTNTDR
LTRGIKEDIANSILIKLNQIGTVTETLEAIELAKKNGYTSVISHRSGETDDPFIADLAVATNAGQIKTGAPSRMDRVAKY
NQLIRISEELYGVSRYPGMGSFYNLEV
>Mature_426_residues
SMIEEIYAREIFDSRGNPTVEVELYTESGAYGFARVPSGASTGVHEALELRDGEDRFGGKGVRKACSKVNEEIGPNLVGM
DVTFQSAIDRALLELDGTDNKENLGANAMLGVSLAAARASAEFLGLPLYQVLGGVSSSTLPIPQMNILNGGEHADNNVDI
QEFMIMPIRANNFQHAMRMGVEIFHALKNVLKDEGLSTSVGDEGGFAPDLKSNKEALEYIITAIEKVGYKPGEQVMLAID
AAASELYQGDKYHLEGEGKSLTADEMIDLYQDLVENYPIISIEDGLSEDDWEGWKKMTEKFQGKIQLVGDDLFVTNTDRL
TRGIKEDIANSILIKLNQIGTVTETLEAIELAKKNGYTSVISHRSGETDDPFIADLAVATNAGQIKTGAPSRMDRVAKYN
QLIRISEELYGVSRYPGMGSFYNLEV

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=437, Percent_Identity=51.7162471395881, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI4503571, Length=428, Percent_Identity=50.9345794392523, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=420, Percent_Identity=49.047619047619, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI301897469, Length=420, Percent_Identity=49.047619047619, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI301897479, Length=418, Percent_Identity=44.9760765550239, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=24.702380952381, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=24.702380952381, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=24.702380952381, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=57.7830188679245, Blast_Score=465, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71995829, Length=440, Percent_Identity=51.8181818181818, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17536383, Length=440, Percent_Identity=51.8181818181818, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32563855, Length=201, Percent_Identity=46.7661691542289, Blast_Score=185, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323985, Length=431, Percent_Identity=48.0278422273782, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324974, Length=431, Percent_Identity=47.7958236658933, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324969, Length=431, Percent_Identity=47.7958236658933, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321693, Length=423, Percent_Identity=48.2269503546099, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=48.0278422273782, Blast_Score=353, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580918, Length=429, Percent_Identity=50.5827505827506, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=429, Percent_Identity=50.5827505827506, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=429, Percent_Identity=50.5827505827506, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=429, Percent_Identity=50.5827505827506, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=50.5827505827506, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=50.5827505827506, Blast_Score=401, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_NATTJ (B2A6Z1)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918170.1
- ProteinModelPortal:   B2A6Z1
- SMR:   B2A6Z1
- GeneID:   6315870
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2015
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46642; Mature: 46511

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 336-336 BINDING 154-154 BINDING 163-163 BINDING 284-284 BINDING 311-311 BINDING 336-336 BINDING 387-387

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMIEEIYAREIFDSRGNPTVEVELYTESGAYGFARVPSGASTGVHEALELRDGEDRFGG
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHCCCCCCCCCC
KGVRKACSKVNEEIGPNLVGMDVTFQSAIDRALLELDGTDNKENLGANAMLGVSLAAARA
HHHHHHHHHHHHHHCCCEEEECEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
SAEFLGLPLYQVLGGVSSSTLPIPQMNILNGGEHADNNVDIQEFMIMPIRANNFQHAMRM
HHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEECCCHHHHHHH
GVEIFHALKNVLKDEGLSTSVGDEGGFAPDLKSNKEALEYIITAIEKVGYKPGEQVMLAI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
DAAASELYQGDKYHLEGEGKSLTADEMIDLYQDLVENYPIISIEDGLSEDDWEGWKKMTE
EHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
KFQGKIQLVGDDLFVTNTDRLTRGIKEDIANSILIKLNQIGTVTETLEAIELAKKNGYTS
HHCCEEEEEECCEEEECHHHHHHHHHHHHHHHHEEEHHCCCCHHHHHHHHHHHHHCCCHH
VISHRSGETDDPFIADLAVATNAGQIKTGAPSRMDRVAKYNQLIRISEELYGVSRYPGMG
HHHCCCCCCCCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SFYNLEV
CEEEECC
>Mature Secondary Structure 
SMIEEIYAREIFDSRGNPTVEVELYTESGAYGFARVPSGASTGVHEALELRDGEDRFGG
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHCCCCCCCCCC
KGVRKACSKVNEEIGPNLVGMDVTFQSAIDRALLELDGTDNKENLGANAMLGVSLAAARA
HHHHHHHHHHHHHHCCCEEEECEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
SAEFLGLPLYQVLGGVSSSTLPIPQMNILNGGEHADNNVDIQEFMIMPIRANNFQHAMRM
HHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEECCCHHHHHHH
GVEIFHALKNVLKDEGLSTSVGDEGGFAPDLKSNKEALEYIITAIEKVGYKPGEQVMLAI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
DAAASELYQGDKYHLEGEGKSLTADEMIDLYQDLVENYPIISIEDGLSEDDWEGWKKMTE
EHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
KFQGKIQLVGDDLFVTNTDRLTRGIKEDIANSILIKLNQIGTVTETLEAIELAKKNGYTS
HHCCEEEEEECCEEEECHHHHHHHHHHHHHHHHEEEHHCCCCHHHHHHHHHHHHHCCCHH
VISHRSGETDDPFIADLAVATNAGQIKTGAPSRMDRVAKYNQLIRISEELYGVSRYPGMG
HHHCCCCCCCCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SFYNLEV
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA