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The map label for this gene is hisF

Identifier: 188586607

GI number: 188586607

Start: 2106099

End: 2106854

Strand: Reverse

Name: hisF

Synonym: Nther_1997

Alternate gene names: 188586607

Gene position: 2106854-2106099 (Counterclockwise)

Preceding gene: 188586608

Following gene: 188586606

Centisome position: 66.56

GC content: 39.55

Gene sequence:

>756_bases
TTGCTAGCTAAAAGAATAATACCTTGTCTTGATGTCAAAGATGGACAAGTAGTCAAGGGTGTAAAATTTAATGAATTAAA
ATTAGCCGGTGACCCGGTAGAACTTGCTCAAAAGTACGACCGGGAAGGTGCCGATGAGCTGGTTTTTTTAGATATTACAG
CATCTATTGAAGATAGGGGGACCATGTTAGAAGTAGTTGAGAAAACTGCCAAAAATGTTTTTATTCCCTTTACTGTAGGT
GGAGGAATTAAAACGGTAGACGATATGAGAAGATTATTGCAAGCAGGTGCTGACAAAGTGTCTATTAATTCTGCAGCTGT
CAAAAACCCGGAATTAATCAGTCAGGGTAGTGATCGTTTTGGAAGCCAGTGTATTGTAGTTGCCATTGATACCAAAAAGA
CCGAGGAAGGTTATCAGGTATTTATCAATGGTGGTACGAAAAATACCGGCATACCATTAATGGATTGGGTAAAAGAGGTG
AAATATAGAGGTGCAGGAGAAATCTTGTTGACCAGTATCGATCATGATGGAGGGAAAAATGGTTATGATAATGATATCAT
CAATCAGGTCGCTCGGGTAAGTAATATTCCTGTAATAGCTTCAGGAGGTGCCGGTACTATAGAAGACTTTTACAATGTGT
TCCATAAGGGACAGGCTAGTGGAGCCCTAGCAGCCTCAGTTTTTCACTTTGAAGAAATATCTGTCAAAGAGTTGAAGAAC
TATCTTAGTGAGAAGGGAGTTGAGGTTCGATTATGA

Upstream 100 bases:

>100_bases
GCACACAACTTAGCAGGAGTGATCCTGGGAAAAGCTTTATATGAAGGGAAAATAGATTTACAAGGGGCACTGGAAACCAT
TAGAAAATGAGGTGATTTTA

Downstream 100 bases:

>100_bases
ATGTAAATTTTTCTCAAGATGGATTGATACCTTGTATTGCCCAGGATGCCAATACTGGAGAAGTATTAATGCTTGCATAC
ATGAGTCAAGAAGCCTTTGA

Product: imidazole glycerol phosphate synthase subunit hisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLAKRIIPCLDVKDGQVVKGVKFNELKLAGDPVELAQKYDREGADELVFLDITASIEDRGTMLEVVEKTAKNVFIPFTVG
GGIKTVDDMRRLLQAGADKVSINSAAVKNPELISQGSDRFGSQCIVVAIDTKKTEEGYQVFINGGTKNTGIPLMDWVKEV
KYRGAGEILLTSIDHDGGKNGYDNDIINQVARVSNIPVIASGGAGTIEDFYNVFHKGQASGALAASVFHFEEISVKELKN
YLSEKGVEVRL

Sequences:

>Translated_251_residues
MLAKRIIPCLDVKDGQVVKGVKFNELKLAGDPVELAQKYDREGADELVFLDITASIEDRGTMLEVVEKTAKNVFIPFTVG
GGIKTVDDMRRLLQAGADKVSINSAAVKNPELISQGSDRFGSQCIVVAIDTKKTEEGYQVFINGGTKNTGIPLMDWVKEV
KYRGAGEILLTSIDHDGGKNGYDNDIINQVARVSNIPVIASGGAGTIEDFYNVFHKGQASGALAASVFHFEEISVKELKN
YLSEKGVEVRL
>Mature_251_residues
MLAKRIIPCLDVKDGQVVKGVKFNELKLAGDPVELAQKYDREGADELVFLDITASIEDRGTMLEVVEKTAKNVFIPFTVG
GGIKTVDDMRRLLQAGADKVSINSAAVKNPELISQGSDRFGSQCIVVAIDTKKTEEGYQVFINGGTKNTGIPLMDWVKEV
KYRGAGEILLTSIDHDGGKNGYDNDIINQVARVSNIPVIASGGAGTIEDFYNVFHKGQASGALAASVFHFEEISVKELKN
YLSEKGVEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=51.3618677042802, Blast_Score=254, Evalue=4e-69,
Organism=Escherichia coli, GI87082028, Length=209, Percent_Identity=31.5789473684211, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=36.5079365079365, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_NATTJ (B2A6X0)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918152.1
- ProteinModelPortal:   B2A6X0
- SMR:   B2A6X0
- GeneID:   6314973
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1997
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27223; Mature: 27223

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKDGQVVKGVKFNELKLAGDPVELAQKYDREGADELVFLDITASIEDRG
CCHHHCCCCCCCCCCCEECCCEECEEEECCCHHHHHHHHCCCCCCCEEEEEEEECCCCCC
TMLEVVEKTAKNVFIPFTVGGGIKTVDDMRRLLQAGADKVSINSAAVKNPELISQGSDRF
HHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEECHHHCCCCHHHHCCCCCC
GSQCIVVAIDTKKTEEGYQVFINGGTKNTGIPLMDWVKEVKYRGAGEILLTSIDHDGGKN
CCEEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCC
GYDNDIINQVARVSNIPVIASGGAGTIEDFYNVFHKGQASGALAASVFHFEEISVKELKN
CCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHH
YLSEKGVEVRL
HHHHCCCEEEC
>Mature Secondary Structure
MLAKRIIPCLDVKDGQVVKGVKFNELKLAGDPVELAQKYDREGADELVFLDITASIEDRG
CCHHHCCCCCCCCCCCEECCCEECEEEECCCHHHHHHHHCCCCCCCEEEEEEEECCCCCC
TMLEVVEKTAKNVFIPFTVGGGIKTVDDMRRLLQAGADKVSINSAAVKNPELISQGSDRF
HHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEECHHHCCCCHHHHCCCCCC
GSQCIVVAIDTKKTEEGYQVFINGGTKNTGIPLMDWVKEVKYRGAGEILLTSIDHDGGKN
CCEEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCC
GYDNDIINQVARVSNIPVIASGGAGTIEDFYNVFHKGQASGALAASVFHFEEISVKELKN
CCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHH
YLSEKGVEVRL
HHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA