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The map label for this gene is hsdM [H]

Identifier: 188586602

GI number: 188586602

Start: 2098698

End: 2100137

Strand: Reverse

Name: hsdM [H]

Synonym: Nther_1992

Alternate gene names: 188586602

Gene position: 2100137-2098698 (Counterclockwise)

Preceding gene: 188586603

Following gene: 188586601

Centisome position: 66.34

GC content: 33.89

Gene sequence:

>1440_bases
ATGACAAATCAAGAAATTATTCAAAAACTTTGGAGTTTATGTGATGTACTTAGAGATGATGGGGTTACTTATCACCAATA
TGTAACAGAGCTAACTTATATTTTATTTCTAAAAATGATGAAAGAAAAGGAAACAGAAGAAGTGATTCCTAAAGAATATC
GTTGGGATTCTTTAACTACAAAACACGGTATGGAACTATACAACCATTATAGACAATTATTACTTGACATTGGTCAATCC
AAAAATAAATTATTAAGTCAGATATATATGAATGCTACAACCAATATAGATGAACCTAAAAATCTCGAAAAGATTATACA
GTCAATAGACAGGTTAGACTGGTATAGTGCTAGAGAAGAAGGGCTTGGAGCCCTTTATGAAGGTTTGTTAGAGAAAAACG
CAAATGAAACTAAGACAGGCGCGGGTCAGTATTTCACACCGCGTCCTCTTATTGATACTATAGTTGAGCTAACTGATCCT
GAACCAGGAGAAAGATGTAATGACCCAGCTGCAGGTACTTTTGGTTTCATGATTGCTGCAGACAGACATGTAAGAAAGAA
AACAGATGACTACTTCTCTTTAAGTCAAAAAGAAGCTGAATTTCAAGTAAAAGAAGCTTTTACAGGTTGTGAGCTAGTTA
AAGATGTTCACAGGCTGGGTTTAATGAATGCGATGCTACATGAATTGGAAGGTGAAATTATTTTAGGTGATACATTATCT
GAAGCTGGAAAGAACTTAAAAAACTATGATGTAGTTCTAACTAATCCACCTTTTGGCACTAAGAAAAGTGGAGAAAGACC
GACTAGGGACGATTTAACTTATACTACAACTAATAAACAGTTAAACTTCCTTCAACACATATATAGATCATTAAAACCAA
ACGGGAAGGCACGTGCTGCTGTAGTATTACCAGATAATGTGTTATTTGAGGATAATACGGGAGCAAAGATTAGAAATGAT
TTAATGGAGAAGTGTAACCTTCATACCATACTGCGCTTACCAACAGGTATCTTTTATGCTCAAGGTGTTAAAACTAATGT
GTTATTCTTTACTAGAGGGCAAACGGATAAAGACAATACCAAAGAAGTATGGGTATATGATTTAAGAACTAACATGGAGA
GTTTAGGGAAAACCAATCCATTGAGAAAAGATCACTTTGATGACTTTATTAAAGCCTATAAAGCCGTAGATAGAACTAAA
ATAAAAAATGAGAGGTTTAATTGCTTTACAAGAGAGGATATTCGTAAGAAAAATGACAGTTTAGATTTAGGTCTGATTCA
GGATGAATCGCTATCAATATATGAAAATTTACCAGATCCAATTGAATCAGCGGAAAAAACAGTATGTAAACTTCAAGAAG
CTGCATCTTTACTTAATGAGGTTATTGATGAATTAAAGGAAACAGGAATTTCTGAATCAGGAGAGGATAAAAATGACTGA

Upstream 100 bases:

>100_bases
GTTATAAGCAATTAAATAAGATTTTTGATGGACAAATAGATATGATTGTTGAAAAAATTAATGAGGAACTATTTAACCAG
GAAGGAAGGGATCACGTCTA

Downstream 100 bases:

>100_bases
TAAGAAGAGCAAAAGAATAGAAGAATTATTAGAAGAAACTATTGTTCATGAAGACGAAGAACCTTATGAACTGCCTAATA
ACTGGGCTTGGGTTGCATTA

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoKI [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTNQEIIQKLWSLCDVLRDDGVTYHQYVTELTYILFLKMMKEKETEEVIPKEYRWDSLTTKHGMELYNHYRQLLLDIGQS
KNKLLSQIYMNATTNIDEPKNLEKIIQSIDRLDWYSAREEGLGALYEGLLEKNANETKTGAGQYFTPRPLIDTIVELTDP
EPGERCNDPAAGTFGFMIAADRHVRKKTDDYFSLSQKEAEFQVKEAFTGCELVKDVHRLGLMNAMLHELEGEIILGDTLS
EAGKNLKNYDVVLTNPPFGTKKSGERPTRDDLTYTTTNKQLNFLQHIYRSLKPNGKARAAVVLPDNVLFEDNTGAKIRND
LMEKCNLHTILRLPTGIFYAQGVKTNVLFFTRGQTDKDNTKEVWVYDLRTNMESLGKTNPLRKDHFDDFIKAYKAVDRTK
IKNERFNCFTREDIRKKNDSLDLGLIQDESLSIYENLPDPIESAEKTVCKLQEAASLLNEVIDELKETGISESGEDKND

Sequences:

>Translated_479_residues
MTNQEIIQKLWSLCDVLRDDGVTYHQYVTELTYILFLKMMKEKETEEVIPKEYRWDSLTTKHGMELYNHYRQLLLDIGQS
KNKLLSQIYMNATTNIDEPKNLEKIIQSIDRLDWYSAREEGLGALYEGLLEKNANETKTGAGQYFTPRPLIDTIVELTDP
EPGERCNDPAAGTFGFMIAADRHVRKKTDDYFSLSQKEAEFQVKEAFTGCELVKDVHRLGLMNAMLHELEGEIILGDTLS
EAGKNLKNYDVVLTNPPFGTKKSGERPTRDDLTYTTTNKQLNFLQHIYRSLKPNGKARAAVVLPDNVLFEDNTGAKIRND
LMEKCNLHTILRLPTGIFYAQGVKTNVLFFTRGQTDKDNTKEVWVYDLRTNMESLGKTNPLRKDHFDDFIKAYKAVDRTK
IKNERFNCFTREDIRKKNDSLDLGLIQDESLSIYENLPDPIESAEKTVCKLQEAASLLNEVIDELKETGISESGEDKND
>Mature_478_residues
TNQEIIQKLWSLCDVLRDDGVTYHQYVTELTYILFLKMMKEKETEEVIPKEYRWDSLTTKHGMELYNHYRQLLLDIGQSK
NKLLSQIYMNATTNIDEPKNLEKIIQSIDRLDWYSAREEGLGALYEGLLEKNANETKTGAGQYFTPRPLIDTIVELTDPE
PGERCNDPAAGTFGFMIAADRHVRKKTDDYFSLSQKEAEFQVKEAFTGCELVKDVHRLGLMNAMLHELEGEIILGDTLSE
AGKNLKNYDVVLTNPPFGTKKSGERPTRDDLTYTTTNKQLNFLQHIYRSLKPNGKARAAVVLPDNVLFEDNTGAKIRNDL
MEKCNLHTILRLPTGIFYAQGVKTNVLFFTRGQTDKDNTKEVWVYDLRTNMESLGKTNPLRKDHFDDFIKAYKAVDRTKI
KNERFNCFTREDIRKKNDSLDLGLIQDESLSIYENLPDPIESAEKTVCKLQEAASLLNEVIDELKETGISESGEDKND

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=511, Percent_Identity=46.3796477495108, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 54972; Mature: 54841

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQEIIQKLWSLCDVLRDDGVTYHQYVTELTYILFLKMMKEKETEEVIPKEYRWDSLTT
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCH
KHGMELYNHYRQLLLDIGQSKNKLLSQIYMNATTNIDEPKNLEKIIQSIDRLDWYSAREE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GLGALYEGLLEKNANETKTGAGQYFTPRPLIDTIVELTDPEPGERCNDPAAGTFGFMIAA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEEC
DRHVRKKTDDYFSLSQKEAEFQVKEAFTGCELVKDVHRLGLMNAMLHELEGEIILGDTLS
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHH
EAGKNLKNYDVVLTNPPFGTKKSGERPTRDDLTYTTTNKQLNFLQHIYRSLKPNGKARAA
HHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCEEE
VVLPDNVLFEDNTGAKIRNDLMEKCNLHTILRLPTGIFYAQGVKTNVLFFTRGQTDKDNT
EEECCCEEEECCCCHHHHHHHHHHCCCHHHHHCCCCHHEECCCCEEEEEEECCCCCCCCC
KEVWVYDLRTNMESLGKTNPLRKDHFDDFIKAYKAVDRTKIKNERFNCFTREDIRKKNDS
CEEEEEEHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
LDLGLIQDESLSIYENLPDPIESAEKTVCKLQEAASLLNEVIDELKETGISESGEDKND
CCEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TNQEIIQKLWSLCDVLRDDGVTYHQYVTELTYILFLKMMKEKETEEVIPKEYRWDSLTT
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCH
KHGMELYNHYRQLLLDIGQSKNKLLSQIYMNATTNIDEPKNLEKIIQSIDRLDWYSAREE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GLGALYEGLLEKNANETKTGAGQYFTPRPLIDTIVELTDPEPGERCNDPAAGTFGFMIAA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEEC
DRHVRKKTDDYFSLSQKEAEFQVKEAFTGCELVKDVHRLGLMNAMLHELEGEIILGDTLS
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHH
EAGKNLKNYDVVLTNPPFGTKKSGERPTRDDLTYTTTNKQLNFLQHIYRSLKPNGKARAA
HHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCEEE
VVLPDNVLFEDNTGAKIRNDLMEKCNLHTILRLPTGIFYAQGVKTNVLFFTRGQTDKDNT
EEECCCEEEECCCCHHHHHHHHHHCCCHHHHHCCCCHHEECCCCEEEEEEECCCCCCCCC
KEVWVYDLRTNMESLGKTNPLRKDHFDDFIKAYKAVDRTKIKNERFNCFTREDIRKKNDS
CEEEEEEHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
LDLGLIQDESLSIYENLPDPIESAEKTVCKLQEAASLLNEVIDELKETGISESGEDKND
CCEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3323532; 7610040; 9278503 [H]