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The map label for this gene is hsdS [H]

Identifier: 188586601

GI number: 188586601

Start: 2097233

End: 2098705

Strand: Reverse

Name: hsdS [H]

Synonym: Nther_1991

Alternate gene names: 188586601

Gene position: 2098705-2097233 (Counterclockwise)

Preceding gene: 188586602

Following gene: 188586600

Centisome position: 66.3

GC content: 29.87

Gene sequence:

>1473_bases
ATGACTGATAAGAAGAGCAAAAGAATAGAAGAATTATTAGAAGAAACTATTGTTCATGAAGACGAAGAACCTTATGAACT
GCCTAATAACTGGGCTTGGGTTGCATTAGACATACTAGCAGAAGAAATTAAAAATGGAACTACAATAAAACAAAGCAAGA
CCAAACCTGGGATACCTGTCACGAGGATAGAATCAATTCAAAACAACGAAATACAACTGGATAGAGTTAGATATATAAGG
GATTTAGATAAAATCAAAAATAATGATTATTATAAAATTGGAGATATTGTTTTAAGCCATATAAATAGCATTGAACATGT
AGGTAAAACTGCTTTGATTAAGGAAGATTATCTACCTTTAATACATGGAATGAATTTATTAAGAATTAGAGTAAATAATA
ATATGATTCTGCCTCAGTTTCTCCAATTATACACGAGGTCCTATAATTTTAGAAAAGCTGTATTGAAAAGAATTAAAATG
GCAGTCAATCAAGTTAGCTTAAATCAAAAAAATTTGAAGCAAATAAGTATACCAATAGCACCGAAAAATGAACAAAGAAG
AATAGTTTATAAAGTTGATCGCCTTTTAAGTAAAATTAATAAAGCAAAAGAATTAATAGGAGAAGCTAAAGAAACTTTTG
AACTACGTCGTGCAGCTATATTAGATAAGGCTTTTAAAGGGGAATTGACGTGGCGTGAGGAAAATCCGAGAGTTGAGTCA
GTAGATACTTTGCTAGCTAAAATAAATTCTGAGAAGAAAACTGATATTAAAAAAAGCCCCAATGGTTTATATGAACTTCC
GGATAATTGGTGTTGGATTGATTTAGGGGAACTAATTTGTCATTCTTCGTATGGAACATCTGCAAAGGCTTATAAAGATA
TAAATGGTTTACCTGTTCTTAGGATGGGTAATATTAAATTAACTGGAAGTATTGATTTAAATGATCTAAAATACCTTCCT
TTTGATCACAAAGATGTAGAAAAATATAAACTAGAAGAATATGATTTATTGTTTAACAGAACAAATAGTTATGAGCTAGT
TGGAAAATCGGCAATTGTTGAGCCAGAACATGCTGGAAAATTTACGTATGCTTCATATTTGATAAAGATCTCGCTATTTT
ACAAAAAGATTTTAGCACCATATATTTGTTATTATATTAATAGTCATATTGGACGAAAATATCTACTATCTACAGTTAAA
CAACAAGTAGGACAGGCTAATATCAATTCAAAGAAACTATCATCATTACCAGTACCACTACCTCCTGAAGAAGAAATTAA
GGAAATTAATAGAATCATGAAGAAAGTAAGTGCAAAGGAGAATAGAATACAAAACTTATTAAATTTGGGAACCTATGTTG
CTGAACTAGAACAATCTATTCTTTCTAAAGCCTTTCGTGGAGAGTTAAATACTAATGATCCTAAAGATGAAAGTGCAATT
GAATTATTAAAAGAAGTTTTAAAAGATAAATAG

Upstream 100 bases:

>100_bases
GAAAAAACAGTATGTAAACTTCAAGAAGCTGCATCTTTACTTAATGAGGTTATTGATGAATTAAAGGAAACAGGAATTTC
TGAATCAGGAGAGGATAAAA

Downstream 100 bases:

>100_bases
GTTTTAACTGCACAAGTTCGTTCAATAAGCAAGGAGTGGTAATTTGTATTATATATTTATCGTGAATGATTATATGTTGG
GGGATCAAGTAATACCTGGC

Product: restriction modification system DNA specificity domain

Products: NA

Alternate protein names: S.EcoKI; Type I restriction enzyme EcoKI specificity protein; S protein [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTDKKSKRIEELLEETIVHEDEEPYELPNNWAWVALDILAEEIKNGTTIKQSKTKPGIPVTRIESIQNNEIQLDRVRYIR
DLDKIKNNDYYKIGDIVLSHINSIEHVGKTALIKEDYLPLIHGMNLLRIRVNNNMILPQFLQLYTRSYNFRKAVLKRIKM
AVNQVSLNQKNLKQISIPIAPKNEQRRIVYKVDRLLSKINKAKELIGEAKETFELRRAAILDKAFKGELTWREENPRVES
VDTLLAKINSEKKTDIKKSPNGLYELPDNWCWIDLGELICHSSYGTSAKAYKDINGLPVLRMGNIKLTGSIDLNDLKYLP
FDHKDVEKYKLEEYDLLFNRTNSYELVGKSAIVEPEHAGKFTYASYLIKISLFYKKILAPYICYYINSHIGRKYLLSTVK
QQVGQANINSKKLSSLPVPLPPEEEIKEINRIMKKVSAKENRIQNLLNLGTYVAELEQSILSKAFRGELNTNDPKDESAI
ELLKEVLKDK

Sequences:

>Translated_490_residues
MTDKKSKRIEELLEETIVHEDEEPYELPNNWAWVALDILAEEIKNGTTIKQSKTKPGIPVTRIESIQNNEIQLDRVRYIR
DLDKIKNNDYYKIGDIVLSHINSIEHVGKTALIKEDYLPLIHGMNLLRIRVNNNMILPQFLQLYTRSYNFRKAVLKRIKM
AVNQVSLNQKNLKQISIPIAPKNEQRRIVYKVDRLLSKINKAKELIGEAKETFELRRAAILDKAFKGELTWREENPRVES
VDTLLAKINSEKKTDIKKSPNGLYELPDNWCWIDLGELICHSSYGTSAKAYKDINGLPVLRMGNIKLTGSIDLNDLKYLP
FDHKDVEKYKLEEYDLLFNRTNSYELVGKSAIVEPEHAGKFTYASYLIKISLFYKKILAPYICYYINSHIGRKYLLSTVK
QQVGQANINSKKLSSLPVPLPPEEEIKEINRIMKKVSAKENRIQNLLNLGTYVAELEQSILSKAFRGELNTNDPKDESAI
ELLKEVLKDK
>Mature_489_residues
TDKKSKRIEELLEETIVHEDEEPYELPNNWAWVALDILAEEIKNGTTIKQSKTKPGIPVTRIESIQNNEIQLDRVRYIRD
LDKIKNNDYYKIGDIVLSHINSIEHVGKTALIKEDYLPLIHGMNLLRIRVNNNMILPQFLQLYTRSYNFRKAVLKRIKMA
VNQVSLNQKNLKQISIPIAPKNEQRRIVYKVDRLLSKINKAKELIGEAKETFELRRAAILDKAFKGELTWREENPRVESV
DTLLAKINSEKKTDIKKSPNGLYELPDNWCWIDLGELICHSSYGTSAKAYKDINGLPVLRMGNIKLTGSIDLNDLKYLPF
DHKDVEKYKLEEYDLLFNRTNSYELVGKSAIVEPEHAGKFTYASYLIKISLFYKKILAPYICYYINSHIGRKYLLSTVKQ
QVGQANINSKKLSSLPVPLPPEEEIKEINRIMKKVSAKENRIQNLLNLGTYVAELEQSILSKAFRGELNTNDPKDESAIE
LLKEVLKDK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=457, Percent_Identity=26.9146608315098, Blast_Score=125, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 56631; Mature: 56500

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKSKRIEELLEETIVHEDEEPYELPNNWAWVALDILAEEIKNGTTIKQSKTKPGIPV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCH
TRIESIQNNEIQLDRVRYIRDLDKIKNNDYYKIGDIVLSHINSIEHVGKTALIKEDYLPL
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IHGMNLLRIRVNNNMILPQFLQLYTRSYNFRKAVLKRIKMAVNQVSLNQKNLKQISIPIA
HCCCEEEEEEECCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHCHHEEECCCC
PKNEQRRIVYKVDRLLSKINKAKELIGEAKETFELRRAAILDKAFKGELTWREENPRVES
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHH
VDTLLAKINSEKKTDIKKSPNGLYELPDNWCWIDLGELICHSSYGTSAKAYKDINGLPVL
HHHHHHHHCCCHHHCCCCCCCCCEECCCCCEEEEHHHHHHCCCCCCCHHHHHCCCCCCEE
RMGNIKLTGSIDLNDLKYLPFDHKDVEKYKLEEYDLLFNRTNSYELVGKSAIVEPEHAGK
EECCEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHCCC
FTYASYLIKISLFYKKILAPYICYYINSHIGRKYLLSTVKQQVGQANINSKKLSSLPVPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC
PPEEEIKEINRIMKKVSAKENRIQNLLNLGTYVAELEQSILSKAFRGELNTNDPKDESAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
ELLKEVLKDK
HHHHHHHCCC
>Mature Secondary Structure 
TDKKSKRIEELLEETIVHEDEEPYELPNNWAWVALDILAEEIKNGTTIKQSKTKPGIPV
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCH
TRIESIQNNEIQLDRVRYIRDLDKIKNNDYYKIGDIVLSHINSIEHVGKTALIKEDYLPL
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IHGMNLLRIRVNNNMILPQFLQLYTRSYNFRKAVLKRIKMAVNQVSLNQKNLKQISIPIA
HCCCEEEEEEECCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHCHHEEECCCC
PKNEQRRIVYKVDRLLSKINKAKELIGEAKETFELRRAAILDKAFKGELTWREENPRVES
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHH
VDTLLAKINSEKKTDIKKSPNGLYELPDNWCWIDLGELICHSSYGTSAKAYKDINGLPVL
HHHHHHHHCCCHHHCCCCCCCCCEECCCCCEEEEHHHHHHCCCCCCCHHHHHCCCCCCEE
RMGNIKLTGSIDLNDLKYLPFDHKDVEKYKLEEYDLLFNRTNSYELVGKSAIVEPEHAGK
EECCEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHCCC
FTYASYLIKISLFYKKILAPYICYYINSHIGRKYLLSTVKQQVGQANINSKKLSSLPVPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC
PPEEEIKEINRIMKKVSAKENRIQNLLNLGTYVAELEQSILSKAFRGELNTNDPKDESAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
ELLKEVLKDK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321; 7610040; 9278503; 7739896 [H]