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The map label for this gene is thiG [H]

Identifier: 188586530

GI number: 188586530

Start: 2011481

End: 2012269

Strand: Direct

Name: thiG [H]

Synonym: Nther_1916

Alternate gene names: 188586530

Gene position: 2011481-2012269 (Clockwise)

Preceding gene: 188586529

Following gene: 188586531

Centisome position: 63.54

GC content: 40.56

Gene sequence:

>789_bases
TTGTCTAATGACATTTTGAAAATTGGCGGTAAAAAATTAACAAATAGGCTGATTGTGGGCACTGGCAAATTCCCTAGGAA
AAGTCTGATTCCACAAGTTTTAGATAGTTCCGGCTCTCAGGCTGTTACAGTTGCTTTAAGGCGCGTTGATTTAAATTCCG
ATGATGAAAATGTCCTGGATTATATCAATCAAGACTGTATTTTGATGCCAAACACCTCAGGAGCTAGGAATGCAGAAGAA
GCAGTAAAAATTGCTCAAATAGCACAAGCTGCTGGATGTGGTAATTGGGTAAAAATTGAAGTTATTAAAGACCAAAAATA
CCTTTTACCAGACAATTCAGAAACTTTAAAAGCTTCAAAAACCCTAGTGGATGAAGGGTTTAAAGTCTTTCCTTATGTTA
GTCCCGAGTTATCAATTGCCCAGGAAATGGAGCAAATTGGAGTTGAAGCTGTAATGCCCTTAGGAGCACCTATTGGTACT
AATAAAGGGCTTGAAACCCGTGAGCTCATTAAAATACTAATATCTGAGATAAATTTACCTATTATTGTTGATGCGGGTAT
CGGTCGGCCATCTCAAGCGGCCGAAGCTATGGAACTAGGCTGTGAAGCTGTGCTAGTAAATACCGCTATTGCTACGGCTG
AAAACCCAGTTTTAATGGCAGAAGCTTTCAGTCAAGCTGTCAAGTCGGGAAGGAAAGCATATTTATCCAAAGCCGCACCC
GTGAAAGAGACTGCTGAAGCTTCTTCACCTTTGACTGGTTTTCTTCGAGACGAAAATGGGGAGGGATAA

Upstream 100 bases:

>100_bases
ATCTTAAACAGGGAAGTAGTTCACAAAGACGAATGGGAAAAGACCCAATTATCAGAAGGTGACGAAATTGAAGTATTAAG
TATGGTTGGAGGTGGTTAAT

Downstream 100 bases:

>100_bases
GTAATGAGTTTTAGAGATTTTATTAAAAAATACCAACAGCTAGACTTTCAGCAAACTTTTCAAGATATCACTCCCCAAAG
GGTTGAAACCGCAATTTATA

Product: thiazole biosynthesis family protein

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSNDILKIGGKKLTNRLIVGTGKFPRKSLIPQVLDSSGSQAVTVALRRVDLNSDDENVLDYINQDCILMPNTSGARNAEE
AVKIAQIAQAAGCGNWVKIEVIKDQKYLLPDNSETLKASKTLVDEGFKVFPYVSPELSIAQEMEQIGVEAVMPLGAPIGT
NKGLETRELIKILISEINLPIIVDAGIGRPSQAAEAMELGCEAVLVNTAIATAENPVLMAEAFSQAVKSGRKAYLSKAAP
VKETAEASSPLTGFLRDENGEG

Sequences:

>Translated_262_residues
MSNDILKIGGKKLTNRLIVGTGKFPRKSLIPQVLDSSGSQAVTVALRRVDLNSDDENVLDYINQDCILMPNTSGARNAEE
AVKIAQIAQAAGCGNWVKIEVIKDQKYLLPDNSETLKASKTLVDEGFKVFPYVSPELSIAQEMEQIGVEAVMPLGAPIGT
NKGLETRELIKILISEINLPIIVDAGIGRPSQAAEAMELGCEAVLVNTAIATAENPVLMAEAFSQAVKSGRKAYLSKAAP
VKETAEASSPLTGFLRDENGEG
>Mature_261_residues
SNDILKIGGKKLTNRLIVGTGKFPRKSLIPQVLDSSGSQAVTVALRRVDLNSDDENVLDYINQDCILMPNTSGARNAEEA
VKIAQIAQAAGCGNWVKIEVIKDQKYLLPDNSETLKASKTLVDEGFKVFPYVSPELSIAQEMEQIGVEAVMPLGAPIGTN
KGLETRELIKILISEINLPIIVDAGIGRPSQAAEAMELGCEAVLVNTAIATAENPVLMAEAFSQAVKSGRKAYLSKAAPV
KETAEASSPLTGFLRDENGEG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=48.8095238095238, Blast_Score=245, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 27936; Mature: 27805

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDILKIGGKKLTNRLIVGTGKFPRKSLIPQVLDSSGSQAVTVALRRVDLNSDDENVLD
CCCCEEECCCHHHCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHH
YINQDCILMPNTSGARNAEEAVKIAQIAQAAGCGNWVKIEVIKDQKYLLPDNSETLKASK
HCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCEEECCCCCCHHHHHH
TLVDEGFKVFPYVSPELSIAQEMEQIGVEAVMPLGAPIGTNKGLETRELIKILISEINLP
HHHHCCCEEEEECCCCHHHHHHHHHHCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
IIVDAGIGRPSQAAEAMELGCEAVLVNTAIATAENPVLMAEAFSQAVKSGRKAYLSKAAP
EEEECCCCCCHHHHHHHHHCCHHEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VKETAEASSPLTGFLRDENGEG
CHHHHHCCCCCEEEEECCCCCC
>Mature Secondary Structure 
SNDILKIGGKKLTNRLIVGTGKFPRKSLIPQVLDSSGSQAVTVALRRVDLNSDDENVLD
CCCEEECCCHHHCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHH
YINQDCILMPNTSGARNAEEAVKIAQIAQAAGCGNWVKIEVIKDQKYLLPDNSETLKASK
HCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCEEECCCCCCHHHHHH
TLVDEGFKVFPYVSPELSIAQEMEQIGVEAVMPLGAPIGTNKGLETRELIKILISEINLP
HHHHCCCEEEEECCCCHHHHHHHHHHCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
IIVDAGIGRPSQAAEAMELGCEAVLVNTAIATAENPVLMAEAFSQAVKSGRKAYLSKAAP
EEEECCCCCCHHHHHHHHHCCHHEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VKETAEASSPLTGFLRDENGEG
CHHHHHCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA