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The map label for this gene is pckA [H]

Identifier: 188586489

GI number: 188586489

Start: 1961707

End: 1963308

Strand: Direct

Name: pckA [H]

Synonym: Nther_1874

Alternate gene names: 188586489

Gene position: 1961707-1963308 (Clockwise)

Preceding gene: 188586487

Following gene: 188586490

Centisome position: 61.97

GC content: 36.52

Gene sequence:

>1602_bases
ATGGTTTACTCACAAAGTATCTCTTTAAAATGTCTTCCGAGAAAACTAAATGACTTAACCTTACAGGGAGTTGCAGATTT
ACCGATTTCAAGTTTAGTTGAAGAAGTGATTGTCAATGGTGAAGGGAAATTAACTTCACAAGGGGCAATTACAGTCAAAA
CTGGCAAATATACCGGGCGATCTCCTAAGGACAAATTCATCGTTGATGAATCAGAGATTAAAGATAAGATAAATTGGGGA
GAAATCAATTGCCCTATTCCTGAATCAACCTTTGACAAGCTTTATGATAAGGTTTTAAATTATCTTAGCGAAAGAAAAAT
TTATAAATTTAACGGAAAAGTTTGTTCCCAAGAAGAATACACCTTGCCAATTCATGTTATTACTGAACGCGCCTGGCACA
ACTTGTTTGCAAAACAGTTATTTATTAATGACAACAAACCAATACACCATAATGATTCTTTATTATATCCTGGTTTTACA
GTTATCAACGCACCAGGGTTCAAAGCAGATCCCAAAATTGACGGAACAAATTCAGAAGTTTTTGTGATAATTTCATTCCG
GAGAAGAATTATTTTGATTGGCGGTACTGAGTATGCTGGAGAAATTAAAAAATCCGTCTTTACAGTACTTAATTGTTTGC
TTCCTGAACGAAATGTCCTTCCTATGCATTGTTCAGCTAATATAGGTAAACATGGTGATATTTCGTTATTTTTTGGATTA
TCGGGTACAGGAAAAACTACATTATCTGCTGATGAAAACAGGTTCTTAGTTGGGGATGATGAACACGGCTGGCATGATAA
TGGCATTTTTAATTTTGAAGGTGGCTGTTATGCTAAATGTGTTAACCTTGATAAAGAAAAGGAACCTCAGATTTATGAAG
CTATAAATTTCGGTACAGTTTTGGAAAATGTAGTGATCAAACGTGAAAATGGTGAACCTGATTTCTTTGATACTAGCTTA
ACTGAAAACACTCGAGCAGCATATCCTTTGGATAATATTCCCGGAGCACTTAATTCGGGTAAAGCCAAAAGTCCTGATGT
CATTATTTTCTTAGCAGCTGATGCTTATGGTGTGCTTCCTCCTGTTTCCAAGCTGACTAAGGAACAGGCCATGTACCACT
TTTTATCCGGGTACACTTCCAAACTAGCAGGTACCGAACGAGGGGTGAGTTACCCAGAAGCCACATTTTCTACTTGCTTT
GGGGCACCATTTATGCCTCTTGATCCATGTGTTTATGCAAAAATGCTAGGAGAAAAAATAGAACGACATAATGTTCAAGT
ATATTTAGTTAATACAGGATGGACAGGTGGACCATACGGAAAAGGAAAGCGTATTGATCTTGAATATACCAGGAACATGA
TCACAGCAATCCAGACTAAAGAAATCGAAAAATCATCTTTTCATCCAGATCCAGTTTTTAATGTGTTAGTCCCAGAGAAT
ATCCCTGGTGTACCTTCAAAGATCTTAACACCACGAAATACTTGGGAAAACAAACTTGAATACGATGATAAGGCTAAAAT
TTTAGCTCAAAAATTCCAGGAAAATTTTAAGCAGTTTACTAATATACCTTCCCAGGTATACGCCGCAGGGCCTAATATAT
AA

Upstream 100 bases:

>100_bases
ATGTTGTATCATAATTTTTGGATTATTCCATCATATTTTTTGAAATATGTCACTTAGGATTACATAACGTAATAGAGATG
TGATTAGGGAGGGGAAAAAT

Downstream 100 bases:

>100_bases
GCCACTTTTAGTACAAATAGGGTGCCATTTATGGCACCCTATTTTCTTATATTCACGCTCTTTATTGGACAAGATAATAA
AAAGATCTTTCAAAACGGAG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MVYSQSISLKCLPRKLNDLTLQGVADLPISSLVEEVIVNGEGKLTSQGAITVKTGKYTGRSPKDKFIVDESEIKDKINWG
EINCPIPESTFDKLYDKVLNYLSERKIYKFNGKVCSQEEYTLPIHVITERAWHNLFAKQLFINDNKPIHHNDSLLYPGFT
VINAPGFKADPKIDGTNSEVFVIISFRRRIILIGGTEYAGEIKKSVFTVLNCLLPERNVLPMHCSANIGKHGDISLFFGL
SGTGKTTLSADENRFLVGDDEHGWHDNGIFNFEGGCYAKCVNLDKEKEPQIYEAINFGTVLENVVIKRENGEPDFFDTSL
TENTRAAYPLDNIPGALNSGKAKSPDVIIFLAADAYGVLPPVSKLTKEQAMYHFLSGYTSKLAGTERGVSYPEATFSTCF
GAPFMPLDPCVYAKMLGEKIERHNVQVYLVNTGWTGGPYGKGKRIDLEYTRNMITAIQTKEIEKSSFHPDPVFNVLVPEN
IPGVPSKILTPRNTWENKLEYDDKAKILAQKFQENFKQFTNIPSQVYAAGPNI

Sequences:

>Translated_533_residues
MVYSQSISLKCLPRKLNDLTLQGVADLPISSLVEEVIVNGEGKLTSQGAITVKTGKYTGRSPKDKFIVDESEIKDKINWG
EINCPIPESTFDKLYDKVLNYLSERKIYKFNGKVCSQEEYTLPIHVITERAWHNLFAKQLFINDNKPIHHNDSLLYPGFT
VINAPGFKADPKIDGTNSEVFVIISFRRRIILIGGTEYAGEIKKSVFTVLNCLLPERNVLPMHCSANIGKHGDISLFFGL
SGTGKTTLSADENRFLVGDDEHGWHDNGIFNFEGGCYAKCVNLDKEKEPQIYEAINFGTVLENVVIKRENGEPDFFDTSL
TENTRAAYPLDNIPGALNSGKAKSPDVIIFLAADAYGVLPPVSKLTKEQAMYHFLSGYTSKLAGTERGVSYPEATFSTCF
GAPFMPLDPCVYAKMLGEKIERHNVQVYLVNTGWTGGPYGKGKRIDLEYTRNMITAIQTKEIEKSSFHPDPVFNVLVPEN
IPGVPSKILTPRNTWENKLEYDDKAKILAQKFQENFKQFTNIPSQVYAAGPNI
>Mature_533_residues
MVYSQSISLKCLPRKLNDLTLQGVADLPISSLVEEVIVNGEGKLTSQGAITVKTGKYTGRSPKDKFIVDESEIKDKINWG
EINCPIPESTFDKLYDKVLNYLSERKIYKFNGKVCSQEEYTLPIHVITERAWHNLFAKQLFINDNKPIHHNDSLLYPGFT
VINAPGFKADPKIDGTNSEVFVIISFRRRIILIGGTEYAGEIKKSVFTVLNCLLPERNVLPMHCSANIGKHGDISLFFGL
SGTGKTTLSADENRFLVGDDEHGWHDNGIFNFEGGCYAKCVNLDKEKEPQIYEAINFGTVLENVVIKRENGEPDFFDTSL
TENTRAAYPLDNIPGALNSGKAKSPDVIIFLAADAYGVLPPVSKLTKEQAMYHFLSGYTSKLAGTERGVSYPEATFSTCF
GAPFMPLDPCVYAKMLGEKIERHNVQVYLVNTGWTGGPYGKGKRIDLEYTRNMITAIQTKEIEKSSFHPDPVFNVLVPEN
IPGVPSKILTPRNTWENKLEYDDKAKILAQKFQENFKQFTNIPSQVYAAGPNI

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=505, Percent_Identity=45.9405940594059, Blast_Score=437, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322950, Length=516, Percent_Identity=46.1240310077519, Blast_Score=443, Evalue=1e-125,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59523; Mature: 59523

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYSQSISLKCLPRKLNDLTLQGVADLPISSLVEEVIVNGEGKLTSQGAITVKTGKYTGR
CCCCCCCCEEECCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCEECCCEEEEEECCCCCC
SPKDKFIVDESEIKDKINWGEINCPIPESTFDKLYDKVLNYLSERKIYKFNGKVCSQEEY
CCCCCEEECHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCC
TLPIHVITERAWHNLFAKQLFINDNKPIHHNDSLLYPGFTVINAPGFKADPKIDGTNSEV
EEEEEEEHHHHHHHHHHHHEEECCCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCCE
FVIISFRRRIILIGGTEYAGEIKKSVFTVLNCLLPERNVLPMHCSANIGKHGDISLFFGL
EEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEE
SGTGKTTLSADENRFLVGDDEHGWHDNGIFNFEGGCYAKCVNLDKEKEPQIYEAINFGTV
CCCCCEEECCCCCEEEECCCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHH
LENVVIKRENGEPDFFDTSLTENTRAAYPLDNIPGALNSGKAKSPDVIIFLAADAYGVLP
HHHHEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
PVSKLTKEQAMYHFLSGYTSKLAGTERGVSYPEATFSTCFGAPFMPLDPCVYAKMLGEKI
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
ERHNVQVYLVNTGWTGGPYGKGKRIDLEYTRNMITAIQTKEIEKSSFHPDPVFNVLVPEN
HHCCEEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
IPGVPSKILTPRNTWENKLEYDDKAKILAQKFQENFKQFTNIPSQVYAAGPNI
CCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEECCCCC
>Mature Secondary Structure
MVYSQSISLKCLPRKLNDLTLQGVADLPISSLVEEVIVNGEGKLTSQGAITVKTGKYTGR
CCCCCCCCEEECCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCEECCCEEEEEECCCCCC
SPKDKFIVDESEIKDKINWGEINCPIPESTFDKLYDKVLNYLSERKIYKFNGKVCSQEEY
CCCCCEEECHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCC
TLPIHVITERAWHNLFAKQLFINDNKPIHHNDSLLYPGFTVINAPGFKADPKIDGTNSEV
EEEEEEEHHHHHHHHHHHHEEECCCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCCE
FVIISFRRRIILIGGTEYAGEIKKSVFTVLNCLLPERNVLPMHCSANIGKHGDISLFFGL
EEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEE
SGTGKTTLSADENRFLVGDDEHGWHDNGIFNFEGGCYAKCVNLDKEKEPQIYEAINFGTV
CCCCCEEECCCCCEEEECCCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHH
LENVVIKRENGEPDFFDTSLTENTRAAYPLDNIPGALNSGKAKSPDVIIFLAADAYGVLP
HHHHEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
PVSKLTKEQAMYHFLSGYTSKLAGTERGVSYPEATFSTCFGAPFMPLDPCVYAKMLGEKI
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
ERHNVQVYLVNTGWTGGPYGKGKRIDLEYTRNMITAIQTKEIEKSSFHPDPVFNVLVPEN
HHCCEEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
IPGVPSKILTPRNTWENKLEYDDKAKILAQKFQENFKQFTNIPSQVYAAGPNI
CCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]