Click here to switch to the map view.

The map label for this gene is ilvD [H]

Identifier: 188586465

GI number: 188586465

Start: 1930031

End: 1931674

Strand: Direct

Name: ilvD [H]

Synonym: Nther_1850

Alternate gene names: 188586465

Gene position: 1930031-1931674 (Clockwise)

Preceding gene: 188586464

Following gene: 188586466

Centisome position: 60.97

GC content: 42.34

Gene sequence:

>1644_bases
ATGAACGAAAAGTTTGTCGGTACTGATGCGATTATGAGACGAGCTATGCTCAAAGGTTGTGGATTCGGTGATGATGATAT
CAAAACCAAACCGCATATTGGGATAGTAAACACTTACAACGAAGGTGCTCCTGGACACGCCCATCTCAAACAATTATCCG
AAGTAATCAAACAAGGAGTTTGGGCTGCAGGGGGCGTTCCCTTTGAGTTTGGAGCTCCCTCTACATGTGGAGATATGATC
GTAGGTGAAGAAGAATTAAAATTTGAACTTGCAGGCAGAGATGTAGTCGCCCAGGCTGTCGAGTATGTTTCAACTGTACA
TCAGTTTGATGGGCTTATATTACTGGCAAGCTGTGATAATATTATCCCCGGTGTTGCTCTAGGAGCTATTAGAATGAACA
TCCCCTCTATCATTTTAACCGGAGGTTCTATGCTGGTCGGTGAATACCAAGGAGAGGAAATTCTGCCCTGCGATGTAGGT
GTTATGACTATGGGTAAAGATGCGGAAAGTGAACGGGTCAAAGAGATTGAAAATGTTGCTTGTATGTGCCCTGGAGCTTG
TTCCACCATGGGAACGGCAAATAGTATGCAAATTATGATGGAAGTACTAGGTTTAACATTACCCGGTGTCTCAACAATCC
CTGCTGTTTATGCTGACAAACAGAGGGCTTCACGCCTAGCAGGAAAAAGAATAGTAGATATGGTCAAGGAAGATTTAAAG
CCAAGTAATGTTTTAACAAGAGAAACCTTTTTAAATGCAGTAACAACAGATATTGCCATGGGTGGTTCTACTAATGTCAT
CCTGCATTTAATTGCCCTTGCAAGAGAAGCAGGAGTAGAATTAACGGTTGATGATTTTGATAGAATTGGCCGAAATGTAC
CTTGTGTTTGTGGTGTAAAACCATCAGGCGATTATACAATAGTAGATTTTCATAACGCCGGTGGTGTGCCCGCTATGTTA
AAAGAACTACAATCTTTACTCTATTTAGATTCAAAAGCTATTACCGGAGAAACATTACAAGAAATCATCAATAAAGCAAG
CAATAAAAATCCCGATGTCATTAGATCTATGGACAATCCTATCACTTCAGATGGTGGTCTAACTATCTTAAGAGGTAATC
TAGCACCTAACAGTGCTATTATCAGATCTTCTTCTGTCCCTGAAAGCATGAAGAAGTTTTCGGGCCGAGCCAGGGTATTT
CATAGAGATCAGGACGGTGCTAAGGCCATTAAAGAAGGCAAAATTCAACCAGGAGATGTTATGGTTATTCGGTACGAGGG
ACCGAAAGGTGCCCCAGGAATGAAAGAAATAATGTTGAGTACCGATGCTCTAGTAGCTCATGGGCTCGACGATAGTGTCG
GACTTGTGACAGACGGTAGATTTTCCGGATTTAACCGCGGCCCCATAGTAGGCCATATAACCCCTGAAGCTTTTGAAGGC
GGCCCTCTGGCCTTGGTAGAAGATGGTGATATCATCTCAGTAGATATTAAAGAAGCTACCCTTACTATTGACATCAGTGA
AGAAGAAATGAAACGACGAGGAGCTAACTGGCAACAACCAGAACCGAAAGTAAAACAAGGAATGATGCGACTATACTCCA
AGATGTGCAGATCTGCTGAAGAAGGAGCAGGTATGACATTATAA

Upstream 100 bases:

>100_bases
AACATCATGTATGGAATTTGGGAAGGGTATGAAGTGGCTAGAACTTTATAAACCAAAACTCTATTACAATTAATAAAAAT
CTAAGATGGGAGGTAGTAAT

Downstream 100 bases:

>100_bases
AAAACAAATATTTTAAAGGAGGATTGCAAATGATCTACAAAGCAAGAGAAGGACAAAAAAGTTATGGTGAACCGATTGGA
ATCTTATTACTTGATTTTCA

Product: Dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MNEKFVGTDAIMRRAMLKGCGFGDDDIKTKPHIGIVNTYNEGAPGHAHLKQLSEVIKQGVWAAGGVPFEFGAPSTCGDMI
VGEEELKFELAGRDVVAQAVEYVSTVHQFDGLILLASCDNIIPGVALGAIRMNIPSIILTGGSMLVGEYQGEEILPCDVG
VMTMGKDAESERVKEIENVACMCPGACSTMGTANSMQIMMEVLGLTLPGVSTIPAVYADKQRASRLAGKRIVDMVKEDLK
PSNVLTRETFLNAVTTDIAMGGSTNVILHLIALAREAGVELTVDDFDRIGRNVPCVCGVKPSGDYTIVDFHNAGGVPAML
KELQSLLYLDSKAITGETLQEIINKASNKNPDVIRSMDNPITSDGGLTILRGNLAPNSAIIRSSSVPESMKKFSGRARVF
HRDQDGAKAIKEGKIQPGDVMVIRYEGPKGAPGMKEIMLSTDALVAHGLDDSVGLVTDGRFSGFNRGPIVGHITPEAFEG
GPLALVEDGDIISVDIKEATLTIDISEEEMKRRGANWQQPEPKVKQGMMRLYSKMCRSAEEGAGMTL

Sequences:

>Translated_547_residues
MNEKFVGTDAIMRRAMLKGCGFGDDDIKTKPHIGIVNTYNEGAPGHAHLKQLSEVIKQGVWAAGGVPFEFGAPSTCGDMI
VGEEELKFELAGRDVVAQAVEYVSTVHQFDGLILLASCDNIIPGVALGAIRMNIPSIILTGGSMLVGEYQGEEILPCDVG
VMTMGKDAESERVKEIENVACMCPGACSTMGTANSMQIMMEVLGLTLPGVSTIPAVYADKQRASRLAGKRIVDMVKEDLK
PSNVLTRETFLNAVTTDIAMGGSTNVILHLIALAREAGVELTVDDFDRIGRNVPCVCGVKPSGDYTIVDFHNAGGVPAML
KELQSLLYLDSKAITGETLQEIINKASNKNPDVIRSMDNPITSDGGLTILRGNLAPNSAIIRSSSVPESMKKFSGRARVF
HRDQDGAKAIKEGKIQPGDVMVIRYEGPKGAPGMKEIMLSTDALVAHGLDDSVGLVTDGRFSGFNRGPIVGHITPEAFEG
GPLALVEDGDIISVDIKEATLTIDISEEEMKRRGANWQQPEPKVKQGMMRLYSKMCRSAEEGAGMTL
>Mature_547_residues
MNEKFVGTDAIMRRAMLKGCGFGDDDIKTKPHIGIVNTYNEGAPGHAHLKQLSEVIKQGVWAAGGVPFEFGAPSTCGDMI
VGEEELKFELAGRDVVAQAVEYVSTVHQFDGLILLASCDNIIPGVALGAIRMNIPSIILTGGSMLVGEYQGEEILPCDVG
VMTMGKDAESERVKEIENVACMCPGACSTMGTANSMQIMMEVLGLTLPGVSTIPAVYADKQRASRLAGKRIVDMVKEDLK
PSNVLTRETFLNAVTTDIAMGGSTNVILHLIALAREAGVELTVDDFDRIGRNVPCVCGVKPSGDYTIVDFHNAGGVPAML
KELQSLLYLDSKAITGETLQEIINKASNKNPDVIRSMDNPITSDGGLTILRGNLAPNSAIIRSSSVPESMKKFSGRARVF
HRDQDGAKAIKEGKIQPGDVMVIRYEGPKGAPGMKEIMLSTDALVAHGLDDSVGLVTDGRFSGFNRGPIVGHITPEAFEG
GPLALVEDGDIISVDIKEATLTIDISEEEMKRRGANWQQPEPKVKQGMMRLYSKMCRSAEEGAGMTL

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=590, Percent_Identity=35.7627118644068, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1788157, Length=510, Percent_Identity=29.4117647058824, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI1786464, Length=496, Percent_Identity=30.4435483870968, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI2367371, Length=489, Percent_Identity=29.4478527607362, Blast_Score=172, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6322476, Length=544, Percent_Identity=36.0294117647059, Blast_Score=348, Evalue=1e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 58526; Mature: 58526

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKFVGTDAIMRRAMLKGCGFGDDDIKTKPHIGIVNTYNEGAPGHAHLKQLSEVIKQGV
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC
WAAGGVPFEFGAPSTCGDMIVGEEELKFELAGRDVVAQAVEYVSTVHQFDGLILLASCDN
CCCCCCCCCCCCCCCCCCCEECHHHHEEEECCHHHHHHHHHHHHHHHHHCCEEEEECCCC
IIPGVALGAIRMNIPSIILTGGSMLVGEYQGEEILPCDVGVMTMGKDAESERVKEIENVA
CCCCHHHHHHHCCCCEEEEECCCEEEEECCCCEECCCCCCEEECCCCCHHHHHHHHHHHH
CMCPGACSTMGTANSMQIMMEVLGLTLPGVSTIPAVYADKQRASRLAGKRIVDMVKEDLK
EECCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSNVLTRETFLNAVTTDIAMGGSTNVILHLIALAREAGVELTVDDFDRIGRNVPCVCGVK
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHCCCCCEEEECC
PSGDYTIVDFHNAGGVPAMLKELQSLLYLDSKAITGETLQEIINKASNKNPDVIRSMDNP
CCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
ITSDGGLTILRGNLAPNSAIIRSSSVPESMKKFSGRARVFHRDQDGAKAIKEGKIQPGDV
CCCCCCEEEEECCCCCCCCEEECCCCHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCE
MVIRYEGPKGAPGMKEIMLSTDALVAHGLDDSVGLVTDGRFSGFNRGPIVGHITPEAFEG
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEECCCCCCC
GPLALVEDGDIISVDIKEATLTIDISEEEMKRRGANWQQPEPKVKQGMMRLYSKMCRSAE
CCEEEEECCCEEEEEEEEEEEEEEECHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCHH
EGAGMTL
CCCCCCC
>Mature Secondary Structure
MNEKFVGTDAIMRRAMLKGCGFGDDDIKTKPHIGIVNTYNEGAPGHAHLKQLSEVIKQGV
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC
WAAGGVPFEFGAPSTCGDMIVGEEELKFELAGRDVVAQAVEYVSTVHQFDGLILLASCDN
CCCCCCCCCCCCCCCCCCCEECHHHHEEEECCHHHHHHHHHHHHHHHHHCCEEEEECCCC
IIPGVALGAIRMNIPSIILTGGSMLVGEYQGEEILPCDVGVMTMGKDAESERVKEIENVA
CCCCHHHHHHHCCCCEEEEECCCEEEEECCCCEECCCCCCEEECCCCCHHHHHHHHHHHH
CMCPGACSTMGTANSMQIMMEVLGLTLPGVSTIPAVYADKQRASRLAGKRIVDMVKEDLK
EECCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSNVLTRETFLNAVTTDIAMGGSTNVILHLIALAREAGVELTVDDFDRIGRNVPCVCGVK
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHCCCCCEEEECC
PSGDYTIVDFHNAGGVPAMLKELQSLLYLDSKAITGETLQEIINKASNKNPDVIRSMDNP
CCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
ITSDGGLTILRGNLAPNSAIIRSSSVPESMKKFSGRARVFHRDQDGAKAIKEGKIQPGDV
CCCCCCEEEEECCCCCCCCEEECCCCHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCE
MVIRYEGPKGAPGMKEIMLSTDALVAHGLDDSVGLVTDGRFSGFNRGPIVGHITPEAFEG
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEECCCCCCC
GPLALVEDGDIISVDIKEATLTIDISEEEMKRRGANWQQPEPKVKQGMMRLYSKMCRSAE
CCEEEEECCCEEEEEEEEEEEEEEECHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCHH
EGAGMTL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA