Click here to switch to the map view.

The map label for this gene is ruvC

Identifier: 188586413

GI number: 188586413

Start: 1863089

End: 1863574

Strand: Reverse

Name: ruvC

Synonym: Nther_1798

Alternate gene names: 188586413

Gene position: 1863574-1863089 (Counterclockwise)

Preceding gene: 188586414

Following gene: 188586412

Centisome position: 58.87

GC content: 39.3

Gene sequence:

>486_bases
ATGATTATAATGGGGTTAGATCCTGGAATAGCAGCATGTGGGTATGGTATAATACATGCTAAAGGTACTAAATTAGAATG
TCTTGCATGCGGTGTTTTACGTACATATAGTGACGAAAGTGCTACAAAGCGCCTGGAATCAATTTATCAAGGAGTTTCAA
ACTTGATAAGTGAATTTTCACCGAATACTGTAGCTGTAGAAAGTTTATTTTTTGCCAAGAACTCTAAGACAGCGATGAAG
GTGGGACAAGCCAAAGGGGTACTAATGCTTGCAGCAGCTCATAACAATTTAGAAATCTTTGAGTATACTCCTTTACAAGT
TAAACAGGGAGTTTGCGGTTATGGTTCGGCTTCTAAGGAACAAGTGCAAAAAATGGTTAAACAGATTCTAAGTTTAGATA
AGATTCCTAAGCCTGATGATGCCGCAGATGCCCTGGCTGTGTCATTGTGCCATTCCCAGGCATACCGCTTGAGAACTAGA
TTATAA

Upstream 100 bases:

>100_bases
AGTTTGAAAGTGAAGAAGAAAAAAATAAAATACTGGAGAGCTATACCAGCTAATAAATCGATCTATTAAAAAACAGGAAA
AATGTTGGGGTGGATTTACT

Downstream 100 bases:

>100_bases
AATTGAACTAGATGGTGAGGGGATAGAAGTGATCACTAGAATAAGAGGCGAGATGTTAGAAATTACACCTGATTATTGTG
TGGTTATGGCCGGAGGTTTA

Product: Holliday junction endonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MIIMGLDPGIAACGYGIIHAKGTKLECLACGVLRTYSDESATKRLESIYQGVSNLISEFSPNTVAVESLFFAKNSKTAMK
VGQAKGVLMLAAAHNNLEIFEYTPLQVKQGVCGYGSASKEQVQKMVKQILSLDKIPKPDDAADALAVSLCHSQAYRLRTR
L

Sequences:

>Translated_161_residues
MIIMGLDPGIAACGYGIIHAKGTKLECLACGVLRTYSDESATKRLESIYQGVSNLISEFSPNTVAVESLFFAKNSKTAMK
VGQAKGVLMLAAAHNNLEIFEYTPLQVKQGVCGYGSASKEQVQKMVKQILSLDKIPKPDDAADALAVSLCHSQAYRLRTR
L
>Mature_161_residues
MIIMGLDPGIAACGYGIIHAKGTKLECLACGVLRTYSDESATKRLESIYQGVSNLISEFSPNTVAVESLFFAKNSKTAMK
VGQAKGVLMLAAAHNNLEIFEYTPLQVKQGVCGYGSASKEQVQKMVKQILSLDKIPKPDDAADALAVSLCHSQAYRLRTR
L

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=136, Percent_Identity=46.3235294117647, Blast_Score=128, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_NATTJ (B2A5L6)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917958.1
- ProteinModelPortal:   B2A5L6
- SMR:   B2A5L6
- GeneID:   6315261
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1798
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17370; Mature: 17370

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIMGLDPGIAACGYGIIHAKGTKLECLACGVLRTYSDESATKRLESIYQGVSNLISEFS
CEEEECCCCHHHHCCCEEECCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
PNTVAVESLFFAKNSKTAMKVGQAKGVLMLAAAHNNLEIFEYTPLQVKQGVCGYGSASKE
CCCEEHHHHHHHCCCCHHHHHCCCCCEEEEEEECCCEEEEEECCHHHHCCCCCCCCCCHH
QVQKMVKQILSLDKIPKPDDAADALAVSLCHSQAYRLRTRL
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIIMGLDPGIAACGYGIIHAKGTKLECLACGVLRTYSDESATKRLESIYQGVSNLISEFS
CEEEECCCCHHHHCCCEEECCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
PNTVAVESLFFAKNSKTAMKVGQAKGVLMLAAAHNNLEIFEYTPLQVKQGVCGYGSASKE
CCCEEHHHHHHHCCCCHHHHHCCCCCEEEEEEECCCEEEEEECCHHHHCCCCCCCCCCHH
QVQKMVKQILSLDKIPKPDDAADALAVSLCHSQAYRLRTRL
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA