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The map label for this gene is ruvA

Identifier: 188586412

GI number: 188586412

Start: 1862416

End: 1863060

Strand: Reverse

Name: ruvA

Synonym: Nther_1797

Alternate gene names: 188586412

Gene position: 1863060-1862416 (Counterclockwise)

Preceding gene: 188586413

Following gene: 188586411

Centisome position: 58.85

GC content: 33.95

Gene sequence:

>645_bases
GTGATCACTAGAATAAGAGGCGAGATGTTAGAAATTACACCTGATTATTGTGTGGTTATGGCCGGAGGTTTAGGATACAA
AATATACATTCCTGATAATTGTCAAGAGGAGATACCGGATCCTGGACAGGAAATTGACTTACATACATATTTATCTGTTC
GAGAAGATGCTATGACTTTGTATGGGTTTACTAGTGGTGAACAGTTGGCAGTTTTTGAATTGATTATGAATGTTTCCGGA
ATAGGCCCTAAAATTGCTTTAGCTTTAGTAGGGACTATACCACCTACAGAATTTTATTTATCTGTACTTAATGATCAGGT
TAATCAGTTAACGAAAGTTCCTGGAATTGGTAAAAAATCAGCTCAAAGGATTATTTTGGAATTAAAAGAAAAAGTTAAAG
ATATAACATCCAAAGATGCTTATCAAGATATATCTGCATCAGAAAAACTGGATAATACAGGTGAAAAATTAGGCATTAGT
ACCAGACACAAACATCTTGATGAATTAAAAGCTGCTTTAAGCAGTTTAGGTTATACTAATCGGGAAATTGAAAAAACAGT
TGATGCAATTCAAGGACAAATAACGGAAGGGCAAGATATGGAAGAACTCTTGCGATTAGCTTTACAAAAATTAAACACGA
AATGA

Upstream 100 bases:

>100_bases
GATGATGCCGCAGATGCCCTGGCTGTGTCATTGTGCCATTCCCAGGCATACCGCTTGAGAACTAGATTATAAAATTGAAC
TAGATGGTGAGGGGATAGAA

Downstream 100 bases:

>100_bases
TGAGGAGGAAAAATAATGTCCTCAGATGAAAGAATTATTACTCGCCAGCAAGTGGGGGAAGACTATCAACTGGATAGCGA
TTTGCGTCCAAGAAAATTTG

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MITRIRGEMLEITPDYCVVMAGGLGYKIYIPDNCQEEIPDPGQEIDLHTYLSVREDAMTLYGFTSGEQLAVFELIMNVSG
IGPKIALALVGTIPPTEFYLSVLNDQVNQLTKVPGIGKKSAQRIILELKEKVKDITSKDAYQDISASEKLDNTGEKLGIS
TRHKHLDELKAALSSLGYTNREIEKTVDAIQGQITEGQDMEELLRLALQKLNTK

Sequences:

>Translated_214_residues
MITRIRGEMLEITPDYCVVMAGGLGYKIYIPDNCQEEIPDPGQEIDLHTYLSVREDAMTLYGFTSGEQLAVFELIMNVSG
IGPKIALALVGTIPPTEFYLSVLNDQVNQLTKVPGIGKKSAQRIILELKEKVKDITSKDAYQDISASEKLDNTGEKLGIS
TRHKHLDELKAALSSLGYTNREIEKTVDAIQGQITEGQDMEELLRLALQKLNTK
>Mature_214_residues
MITRIRGEMLEITPDYCVVMAGGLGYKIYIPDNCQEEIPDPGQEIDLHTYLSVREDAMTLYGFTSGEQLAVFELIMNVSG
IGPKIALALVGTIPPTEFYLSVLNDQVNQLTKVPGIGKKSAQRIILELKEKVKDITSKDAYQDISASEKLDNTGEKLGIS
TRHKHLDELKAALSSLGYTNREIEKTVDAIQGQITEGQDMEELLRLALQKLNTK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=215, Percent_Identity=33.4883720930233, Blast_Score=124, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_NATTJ (B2A5L5)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917957.1
- ProteinModelPortal:   B2A5L5
- SMR:   B2A5L5
- GeneID:   6315260
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1797
- HOGENOM:   HBG635309
- OMA:   LFGFAQK
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 23729; Mature: 23729

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRIRGEMLEITPDYCVVMAGGLGYKIYIPDNCQEEIPDPGQEIDLHTYLSVREDAMTL
CCCCCCCCCEEECCCEEEEEECCCEEEEEECCCCHHHCCCCCCCEEHHHHHHHHHHHEEE
YGFTSGEQLAVFELIMNVSGIGPKIALALVGTIPPTEFYLSVLNDQVNQLTKVPGIGKKS
EECCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
AQRIILELKEKVKDITSKDAYQDISASEKLDNTGEKLGISTRHKHLDELKAALSSLGYTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCCCC
REIEKTVDAIQGQITEGQDMEELLRLALQKLNTK
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITRIRGEMLEITPDYCVVMAGGLGYKIYIPDNCQEEIPDPGQEIDLHTYLSVREDAMTL
CCCCCCCCCEEECCCEEEEEECCCEEEEEECCCCHHHCCCCCCCEEHHHHHHHHHHHEEE
YGFTSGEQLAVFELIMNVSGIGPKIALALVGTIPPTEFYLSVLNDQVNQLTKVPGIGKKS
EECCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
AQRIILELKEKVKDITSKDAYQDISASEKLDNTGEKLGISTRHKHLDELKAALSSLGYTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCCCC
REIEKTVDAIQGQITEGQDMEELLRLALQKLNTK
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA