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The map label for this gene is miaB

Identifier: 188586121

GI number: 188586121

Start: 1566281

End: 1567636

Strand: Direct

Name: miaB

Synonym: Nther_1495

Alternate gene names: 188586121

Gene position: 1566281-1567636 (Clockwise)

Preceding gene: 188586119

Following gene: 188586122

Centisome position: 49.48

GC content: 35.99

Gene sequence:

>1356_bases
ATGACACTTTATCAACTAGGAAATGGAAAAAAATTTTATACTTTGACATTCGGTTGTCAGATGAACGAACATGATTCTGA
AGTTTTGGCAGGAATGTTAGATCAAATGGGATTTGAAAAGGCTGCCTCTGAAGAAGAGGCGGATCTATTAATTATAAATA
CATGTGCGGTTAGAGAAAAAGCGGAACAAAAGGTTTTGGGCAAAATCGGAACTCTAAGATATTTAAAAGAAAACAAACCC
GATATGAAGATAGCCATTGGAGGATGCATGGTTCAGCAGGAGCATGTAGCCAATAAAATTTATCGTGATTTCACCCATGT
GGATATAATTTTTGGTACTCATAATATAAATAGATTCCCTCAGCTGCTCGAACATGTCATGCAAAAAGGGAAACGTGTTA
AAGAAATTTCTCAAGACGATAGTCAGGTATTTGAAAACTTGCCTCATAAAAGGGAAGATTCTATTAAGGCATGGGTAGTA
ATCAGTTACGGTTGCGATAATTATTGTAAATACTGTATAGTACCATATGTCCGAGGACAGCAAAGAAGTCGTGATCCAGA
ACATATTAAATACGAAGTGGAAAAGCTAGCTAAAGAGGGGTTAAAAGAAATAACTCTTTTAGGCCAAAATGTTAATAGCT
ATGGTAAAGATTTGGATCAGAATATTAGCTTTACTAATTTACTGGAAGAACTGTCTAAAATTGAAGGCATAGAACGGATC
CGGTTTATGACTAGTCATCCCAAGGATTTTGATAAAGAATTAATTACTACGCTAAAAGAATCAAACAAGATCTGTGAGCA
TTTTCATTTACCAGTACAAGCTGGAAGTAATAAAATACTTAAAAAGATGGGCCGTGGATATACTCGAGAACATTACGTAG
ATATTGTCAATGATATCAGAGCTGAACTCCCCAATGCTAGTATAACTACAGATATAATTGTAGGTTATCCCGGGGAAGAG
GAAGAAGATTTTCAAGAAACCCTTGACCTAGTTCAAAATGTGAAATTTGATAGTGCTTTTACATTCGTGTATTCTAAAAG
AAGTGGTACACCAGCCGCAGAAATGGCTGAACAAGTTGATGAACAAACAAAAAAAGGACGAATCCAAAAGTTGATTTCGG
TACAACAAGAAATATCAGAACAGAGAAATAAAGATTTAGAGAATACCGTTCAAAGAATTCTTGTGGAGGGTGTCAGTAAA
AATAACGAGGATATGCTGTCCGGCAGGACGAGAACGGATAAATTAGTACACTTTCCAGGCGATAAAGAGCTAATTGGGGA
ACTAGTTGATGTTAAAATTACTCGAGGACACAGTTGGAATTTGTACGGAGAGATATTTGAGGATAGTCTAACATGA

Upstream 100 bases:

>100_bases
ATTAATTTTGGATTCAGTTATAGTGTTTACAGGATTAATGTTTTTTCATTCCTTTTTTGATATAATATAAAAATGCTATA
ATCATACTGGAGGTGAGATT

Downstream 100 bases:

>100_bases
TATCATACCAGGTCAGTTTAGTTGATAGGGGGAATTGTTATGGCTAAAAACACCCGTAAAATTACACCTATGATCCAACA
ATATTTAGATATCAAGGAAG

Product: tRNA-i(6)A37 thiotransferase enzyme MiaB

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTLYQLGNGKKFYTLTFGCQMNEHDSEVLAGMLDQMGFEKAASEEEADLLIINTCAVREKAEQKVLGKIGTLRYLKENKP
DMKIAIGGCMVQQEHVANKIYRDFTHVDIIFGTHNINRFPQLLEHVMQKGKRVKEISQDDSQVFENLPHKREDSIKAWVV
ISYGCDNYCKYCIVPYVRGQQRSRDPEHIKYEVEKLAKEGLKEITLLGQNVNSYGKDLDQNISFTNLLEELSKIEGIERI
RFMTSHPKDFDKELITTLKESNKICEHFHLPVQAGSNKILKKMGRGYTREHYVDIVNDIRAELPNASITTDIIVGYPGEE
EEDFQETLDLVQNVKFDSAFTFVYSKRSGTPAAEMAEQVDEQTKKGRIQKLISVQQEISEQRNKDLENTVQRILVEGVSK
NNEDMLSGRTRTDKLVHFPGDKELIGELVDVKITRGHSWNLYGEIFEDSLT

Sequences:

>Translated_451_residues
MTLYQLGNGKKFYTLTFGCQMNEHDSEVLAGMLDQMGFEKAASEEEADLLIINTCAVREKAEQKVLGKIGTLRYLKENKP
DMKIAIGGCMVQQEHVANKIYRDFTHVDIIFGTHNINRFPQLLEHVMQKGKRVKEISQDDSQVFENLPHKREDSIKAWVV
ISYGCDNYCKYCIVPYVRGQQRSRDPEHIKYEVEKLAKEGLKEITLLGQNVNSYGKDLDQNISFTNLLEELSKIEGIERI
RFMTSHPKDFDKELITTLKESNKICEHFHLPVQAGSNKILKKMGRGYTREHYVDIVNDIRAELPNASITTDIIVGYPGEE
EEDFQETLDLVQNVKFDSAFTFVYSKRSGTPAAEMAEQVDEQTKKGRIQKLISVQQEISEQRNKDLENTVQRILVEGVSK
NNEDMLSGRTRTDKLVHFPGDKELIGELVDVKITRGHSWNLYGEIFEDSLT
>Mature_450_residues
TLYQLGNGKKFYTLTFGCQMNEHDSEVLAGMLDQMGFEKAASEEEADLLIINTCAVREKAEQKVLGKIGTLRYLKENKPD
MKIAIGGCMVQQEHVANKIYRDFTHVDIIFGTHNINRFPQLLEHVMQKGKRVKEISQDDSQVFENLPHKREDSIKAWVVI
SYGCDNYCKYCIVPYVRGQQRSRDPEHIKYEVEKLAKEGLKEITLLGQNVNSYGKDLDQNISFTNLLEELSKIEGIERIR
FMTSHPKDFDKELITTLKESNKICEHFHLPVQAGSNKILKKMGRGYTREHYVDIVNDIRAELPNASITTDIIVGYPGEEE
EDFQETLDLVQNVKFDSAFTFVYSKRSGTPAAEMAEQVDEQTKKGRIQKLISVQQEISEQRNKDLENTVQRILVEGVSKN
NEDMLSGRTRTDKLVHFPGDKELIGELVDVKITRGHSWNLYGEIFEDSLT

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=484, Percent_Identity=35.3305785123967, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI28872784, Length=484, Percent_Identity=35.3305785123967, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI93277076, Length=454, Percent_Identity=29.7356828193833, Blast_Score=178, Evalue=1e-44,
Organism=Escherichia coli, GI1786882, Length=444, Percent_Identity=41.4414414414414, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1787057, Length=452, Percent_Identity=29.2035398230088, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17553146, Length=475, Percent_Identity=32.6315789473684, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI71996771, Length=331, Percent_Identity=28.3987915407855, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21356207, Length=485, Percent_Identity=33.6082474226804, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI19922432, Length=451, Percent_Identity=28.3813747228381, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_NATTJ (B2A3X6)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917666.1
- ProteinModelPortal:   B2A3X6
- SMR:   B2A3X6
- GeneID:   6314831
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1495
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR002792
- Gene3D:   G3DSA:3.40.50.280
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004; SSF52242 Cbl-bd

EC number: NA

Molecular weight: Translated: 51725; Mature: 51594

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLYQLGNGKKFYTLTFGCQMNEHDSEVLAGMLDQMGFEKAASEEEADLLIINTCAVREK
CEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHCHHHHCCCCCCCEEEEEHHHHHHH
AEQKVLGKIGTLRYLKENKPDMKIAIGGCMVQQEHVANKIYRDFTHVDIIFGTHNINRFP
HHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHH
QLLEHVMQKGKRVKEISQDDSQVFENLPHKREDSIKAWVVISYGCDNYCKYCIVPYVRGQ
HHHHHHHHCCHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCC
QRSRDPEHIKYEVEKLAKEGLKEITLLGQNVNSYGKDLDQNISFTNLLEELSKIEGIERI
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHCCCCHHHHHHHHHHHHHHHHH
RFMTSHPKDFDKELITTLKESNKICEHFHLPVQAGSNKILKKMGRGYTREHYVDIVNDIR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHCCCCCHHHHHHHHHHHH
AELPNASITTDIIVGYPGEEEEDFQETLDLVQNVKFDSAFTFVYSKRSGTPAAEMAEQVD
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHH
EQTKKGRIQKLISVQQEISEQRNKDLENTVQRILVEGVSKNNEDMLSGRTRTDKLVHFPG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEECCC
DKELIGELVDVKITRGHSWNLYGEIFEDSLT
CHHHHHHHHCEEEECCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
TLYQLGNGKKFYTLTFGCQMNEHDSEVLAGMLDQMGFEKAASEEEADLLIINTCAVREK
EEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHCHHHHCCCCCCCEEEEEHHHHHHH
AEQKVLGKIGTLRYLKENKPDMKIAIGGCMVQQEHVANKIYRDFTHVDIIFGTHNINRFP
HHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHH
QLLEHVMQKGKRVKEISQDDSQVFENLPHKREDSIKAWVVISYGCDNYCKYCIVPYVRGQ
HHHHHHHHCCHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCC
QRSRDPEHIKYEVEKLAKEGLKEITLLGQNVNSYGKDLDQNISFTNLLEELSKIEGIERI
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHCCCCHHHHHHHHHHHHHHHHH
RFMTSHPKDFDKELITTLKESNKICEHFHLPVQAGSNKILKKMGRGYTREHYVDIVNDIR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHCCCCCHHHHHHHHHHHH
AELPNASITTDIIVGYPGEEEEDFQETLDLVQNVKFDSAFTFVYSKRSGTPAAEMAEQVD
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHH
EQTKKGRIQKLISVQQEISEQRNKDLENTVQRILVEGVSKNNEDMLSGRTRTDKLVHFPG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEECCC
DKELIGELVDVKITRGHSWNLYGEIFEDSLT
CHHHHHHHHCEEEECCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA