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The map label for this gene is yqjC [H]

Identifier: 188586114

GI number: 188586114

Start: 1560152

End: 1560556

Strand: Direct

Name: yqjC [H]

Synonym: Nther_1488

Alternate gene names: 188586114

Gene position: 1560152-1560556 (Clockwise)

Preceding gene: 188586113

Following gene: 188586115

Centisome position: 49.29

GC content: 40.25

Gene sequence:

>405_bases
ATGATTGATAAACTAGATCATATTGGTATTGCAGTGAAAGATCTGCAAGAAGCCCTGGACTTTTATGAAAATCAATTACA
AATTGAAGCTACCGGCACGGAAGAGGTTTCCGAACAAAAAGTCAAAGTGGCTTTCTTACCTGTAGGTGATACAAAATTGG
AATTACTGGAGGGGACTACAGAAGACAGCCCCATAAATAAGTTTATTGAGAAAAAAGGTGCTGGTGTCCAACATATAGCC
CTTCGCGTGGACGACATAGAACAAAGCTTGGAAGACTTAAAATCAAAAGGTGTTAAGCTGATTGATGAAAAGCCAAGATA
TGGTGCAGGTGGGGCAAAGATTGCCTTTGTACATCCAAAAAGTACGGGTGGCACTTTAATAGAGCTTTGCGAGCGTGAAG
AATAA

Upstream 100 bases:

>100_bases
TGAAAGAACAAGGAGTAGCAGAAGTATTTACTCCAGGTACTACTAGTCAGGAAATTATTGACTTTATAGAGAAAAATGTG
GATAATAAGGAGGTATCTTC

Downstream 100 bases:

>100_bases
CCCCAATTAAGGAGGTCATCCATATGGCAACTAATGATAAAGACAAATTGGAAATGTTACAGGAAGAAAAAGAAAAGATA
TCTAAAGGCGGAGGAGAAAA

Product: methylmalonyl-CoA epimerase

Products: (R)-S-Lactoylglutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MIDKLDHIGIAVKDLQEALDFYENQLQIEATGTEEVSEQKVKVAFLPVGDTKLELLEGTTEDSPINKFIEKKGAGVQHIA
LRVDDIEQSLEDLKSKGVKLIDEKPRYGAGGAKIAFVHPKSTGGTLIELCEREE

Sequences:

>Translated_134_residues
MIDKLDHIGIAVKDLQEALDFYENQLQIEATGTEEVSEQKVKVAFLPVGDTKLELLEGTTEDSPINKFIEKKGAGVQHIA
LRVDDIEQSLEDLKSKGVKLIDEKPRYGAGGAKIAFVHPKSTGGTLIELCEREE
>Mature_134_residues
MIDKLDHIGIAVKDLQEALDFYENQLQIEATGTEEVSEQKVKVAFLPVGDTKLELLEGTTEDSPINKFIEKKGAGVQHIA
LRVDDIEQSLEDLKSKGVKLIDEKPRYGAGGAKIAFVHPKSTGGTLIELCEREE

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI188035928, Length=129, Percent_Identity=40.3100775193798, Blast_Score=106, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17506387, Length=132, Percent_Identity=38.6363636363636, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR018146
- InterPro:   IPR017515 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5 [C]

Molecular weight: Translated: 14762; Mature: 14762

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKLDHIGIAVKDLQEALDFYENQLQIEATGTEEVSEQKVKVAFLPVGDTKLELLEGTT
CCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHCCEEEEEEEECCCCEEEEECCCC
EDSPINKFIEKKGAGVQHIALRVDDIEQSLEDLKSKGVKLIDEKPRYGAGGAKIAFVHPK
CCCHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEECCC
STGGTLIELCEREE
CCCCHHHHHHCCCC
>Mature Secondary Structure
MIDKLDHIGIAVKDLQEALDFYENQLQIEATGTEEVSEQKVKVAFLPVGDTKLELLEGTT
CCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHCCEEEEEEEECCCCEEEEECCCC
EDSPINKFIEKKGAGVQHIALRVDDIEQSLEDLKSKGVKLIDEKPRYGAGGAKIAFVHPK
CCCHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEECCC
STGGTLIELCEREE
CCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: Reduced glutathione; Methylglyoxal [C]

Specific reaction: Reduced glutathione + Methylglyoxal --> (R)-S-Lactoylglutathione [C]

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]