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The map label for this gene is bhbA [H]

Identifier: 188586113

GI number: 188586113

Start: 1559748

End: 1560155

Strand: Direct

Name: bhbA [H]

Synonym: Nther_1487

Alternate gene names: 188586113

Gene position: 1559748-1560155 (Clockwise)

Preceding gene: 188586112

Following gene: 188586114

Centisome position: 49.27

GC content: 38.97

Gene sequence:

>408_bases
TTGTCTCCCAAAAGAGTGGTTGTGGCAAAACCAGGTTTAGATGGACATGATAGAGGTGCGAAATTTATCGCAAGAGCCTT
AAGAGATGCAGGAATGGAAGTAATTTATACTGGGCTTAGACAAACTCCAGAACAAATAGCATCTATTACATTACAGGAAG
ATGCTCAAGTTTTGGCATTAAGCTGCTTATCCGGTGCTCATAATGAGTTATTTCCCCAGATTGTATCTATGTTGAAGGAT
GAAGGTGCAGAAGATGTCTTGGTCTTAGGAGGGGGGATTATTCCTGAAGAAGATATCCCTTATCTGAAAGAACAAGGAGT
AGCAGAAGTATTTACTCCAGGTACTACTAGTCAGGAAATTATTGACTTTATAGAGAAAAATGTGGATAATAAGGAGGTAT
CTTCATGA

Upstream 100 bases:

>100_bases
GTGGAGCTTTACGAGAAGTATTTGGAGAATATGAAGCTGACAGCTATATCTAAAATTACCAGTTTAATTAAAACATAACA
TAAAAGGAGGTTATGTTAAC

Downstream 100 bases:

>100_bases
TTGATAAACTAGATCATATTGGTATTGCAGTGAAAGATCTGCAAGAAGCCCTGGACTTTTATGAAAATCAATTACAAATT
GAAGCTACCGGCACGGAAGA

Product: cobalamin B12-binding domain protein

Products: NA

Alternate protein names: MCM [H]

Number of amino acids: Translated: 135; Mature: 134

Protein sequence:

>135_residues
MSPKRVVVAKPGLDGHDRGAKFIARALRDAGMEVIYTGLRQTPEQIASITLQEDAQVLALSCLSGAHNELFPQIVSMLKD
EGAEDVLVLGGGIIPEEDIPYLKEQGVAEVFTPGTTSQEIIDFIEKNVDNKEVSS

Sequences:

>Translated_135_residues
MSPKRVVVAKPGLDGHDRGAKFIARALRDAGMEVIYTGLRQTPEQIASITLQEDAQVLALSCLSGAHNELFPQIVSMLKD
EGAEDVLVLGGGIIPEEDIPYLKEQGVAEVFTPGTTSQEIIDFIEKNVDNKEVSS
>Mature_134_residues
SPKRVVVAKPGLDGHDRGAKFIARALRDAGMEVIYTGLRQTPEQIASITLQEDAQVLALSCLSGAHNELFPQIVSMLKDE
GAEDVLVLGGGIIPEEDIPYLKEQGVAEVFTPGTTSQEIIDFIEKNVDNKEVSS

Specific function: Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA during the polyhydroxyalkanoate degradation pathway [H]

COG id: COG2185

COG function: function code I; Methylmalonyl-CoA mutase, C-terminal domain/subunit (cobalamin-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=134, Percent_Identity=40.2985074626866, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1789284, Length=129, Percent_Identity=40.3100775193798, Blast_Score=110, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25144500, Length=128, Percent_Identity=42.1875, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 14578; Mature: 14447

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPKRVVVAKPGLDGHDRGAKFIARALRDAGMEVIYTGLRQTPEQIASITLQEDAQVLAL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SCLSGAHNELFPQIVSMLKDEGAEDVLVLGGGIIPEEDIPYLKEQGVAEVFTPGTTSQEI
HHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHCCCCEEECCCCCHHHH
IDFIEKNVDNKEVSS
HHHHHCCCCCCCCCC
>Mature Secondary Structure 
SPKRVVVAKPGLDGHDRGAKFIARALRDAGMEVIYTGLRQTPEQIASITLQEDAQVLAL
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SCLSGAHNELFPQIVSMLKDEGAEDVLVLGGGIIPEEDIPYLKEQGVAEVFTPGTTSQEI
HHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHCCCCEEECCCCCHHHH
IDFIEKNVDNKEVSS
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9889346; 11481431 [H]