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The map label for this gene is infB

Identifier: 188586070

GI number: 188586070

Start: 1511187

End: 1513208

Strand: Direct

Name: infB

Synonym: Nther_1444

Alternate gene names: 188586070

Gene position: 1511187-1513208 (Clockwise)

Preceding gene: 188586069

Following gene: 188586071

Centisome position: 47.74

GC content: 36.5

Gene sequence:

>2022_bases
ATGGAAAAAATAAGAGTATATCAATTAGCTAAAGAAATTGGACTAGAAAGCAAGGAACTAGTGACGACTTTACAGGAATT
GGATATATCTGTAAAGAATCATATGAGTACTCTTAGTGAAGAAGAAGCTGATACAGTTAGAGAATTGTATACAGAATCAT
CTGCAAAAGATGAAGATAACAAAGATGAAACATCACAACAACAAGATGAAAAACAAGAGGATAAGAAAGAACAGGGTACT
ACAACAGATAAACCTTTAGTAGCTCCACCTATTACAGTCGGAGAATTGGCAGAGCAAATAGGTGTAGAATCAACGAGTAT
AATCACCAAATTAATTAGTAAGGGTGTAATGGCTAATATTAATCAAAATTTAGAAGAAGATTCTTTAGAGTACCTTGCAC
AAGAGTTCGATTTTGTAGTAACACAAGAAGAACCTGAGGAAGATGAAAAGTCTGAAGAAGATAGAATCATTGAACAAATA
AGAAGTGAAGAACCAAAAGGAGAACCACTTGAAAGAGCTCCTGTTGTTACAGTTATGGGGCATGTTGATCACGGTAAGAC
ATCAATATTGGACTCTATTAGAAAAACGAAAAGTATAGAAAAAGAAGCGGGAGGAATAACACAGCATATAGGTGCTTCTC
GTGTTCTTTATAATGATAAAACCGTTGTGTTTTTGGACACTCCAGGGCACGAGGCTTTTACTGAAATGAGGGCCCGTGGT
GCTCATGTTACCGATATAGCTATCTTAGTGGTAGCAGCAGATGATGGTGTTATGCCTCAAACAGTAGAAGCAATCAATCA
CGCCAAGTCTGCCGGAGTCCCTATCATTGTTGCGATAAATAAAACTGATAAGCCAGATGCAAATCCGGATAGAGTTAAAC
AAGAACTTACTGAATACAACTTGGTTACCGAAGAATGGGGTGGTGACACTATCTGCGTTTCAGTAAGTGCTGTTCAAAAT
GAAGGTATAGATGAGCTCTTGGAAATGGTTCAGTTAGTAGCAGAAATGAATGAATTGACTTCTTATCCGCAAAATACTGG
TAAAGGAACGGTAATTGAAGCAAAACTGGATAAAGGTAGAGGTCCAGTAGCTACAGTATTAGTTGAGGACGGCACCTTAA
AAGTAGGGGACAGTGTTTTATGTGGTAGTACTTTCGGAAATATCAGAGCAATGGTAGATGATCGAGGTAAACGCTTAAAA
CAAGCTAATCCTGTAACACCTGTAGAAATTTTGGGACTAGAAAACATTCCTGAAGCTGGTGATGAATTCCAGGTTGTACC
AGATGAAAAAATGGCTAGAGAGATTGCCAAGAAAAAGCAGGAAAAAGAAAGGGAAGAAAAGTTAAAACGTTCCCAGTCTG
TATCGTTAGATAATTTATTTGATCAGATTAAAGAAGGTGAACAAAAGGAATTAAATATAATTGTCAAAGGCGATGTAAGA
GGGTCAGTTGAGGCTTTGAGAGAAAGTCTTTTAAAACTAAATGATAATGAACACGAAATCAAGGTGAATGTTATTCATGC
AGGTGTAGGTACTGTTTCAGAAAGTGATATTATGCTAGCAGTAGCTTCCAATGCTATTATTATAGGTTTTAATGTCAGAC
CTGATCCAAATGCCAGAAAAGCTGCTGAACGCGAACAAATTGACATGAGGGTTTATCGAGTGATTTACGAAGCTATAGAA
GATGTGAAAGCTGCTATGGCGGGCTTGTTAGATCCAGAATATAAAGAAGTTGTTCAAGGGCAAATAGAGGTAAGGCAATT
ATTCAAGGTATCTAGAATAGGTACCATTGCGGGTTGTTATGTTACCGATGGTTATATTAGAAATAATTCTTATATCAGAG
TGATTCGTGATGGAAGAGTTATCCATGAAGGTAATATTAAGAATTTAAAACGATTTCAAGATGATGTCAAAGAAGTACAA
CAAGGTTACGAATGTGGAATACTATTAGAGAAATTTAACGATCTAAAAGAAGGAGATATTTTTGAAGCTTATACCTATGA
AGAAATTTCTCCAGACGTTTAA

Upstream 100 bases:

>100_bases
TAAACTTCTCAGTCTTCTGAACGACAATTAGCTGTGGCCACCCCTTATTCTAAAACTATGACAACGCTTGAGGAGGGCAT
TAAAATGGAGGTGTCTTAAT

Downstream 100 bases:

>100_bases
AGCTTAGTTAGTTCACTATGAAAATAGATGGATGGTGGTATTATGAGCGGAATGAGAAAAGAAAAATTGCAAGAAGAACT
TAAAAAAATTATTAGTGATA

Product: translation initiation factor 2 (bIF-2)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MEKIRVYQLAKEIGLESKELVTTLQELDISVKNHMSTLSEEEADTVRELYTESSAKDEDNKDETSQQQDEKQEDKKEQGT
TTDKPLVAPPITVGELAEQIGVESTSIITKLISKGVMANINQNLEEDSLEYLAQEFDFVVTQEEPEEDEKSEEDRIIEQI
RSEEPKGEPLERAPVVTVMGHVDHGKTSILDSIRKTKSIEKEAGGITQHIGASRVLYNDKTVVFLDTPGHEAFTEMRARG
AHVTDIAILVVAADDGVMPQTVEAINHAKSAGVPIIVAINKTDKPDANPDRVKQELTEYNLVTEEWGGDTICVSVSAVQN
EGIDELLEMVQLVAEMNELTSYPQNTGKGTVIEAKLDKGRGPVATVLVEDGTLKVGDSVLCGSTFGNIRAMVDDRGKRLK
QANPVTPVEILGLENIPEAGDEFQVVPDEKMAREIAKKKQEKEREEKLKRSQSVSLDNLFDQIKEGEQKELNIIVKGDVR
GSVEALRESLLKLNDNEHEIKVNVIHAGVGTVSESDIMLAVASNAIIIGFNVRPDPNARKAAEREQIDMRVYRVIYEAIE
DVKAAMAGLLDPEYKEVVQGQIEVRQLFKVSRIGTIAGCYVTDGYIRNNSYIRVIRDGRVIHEGNIKNLKRFQDDVKEVQ
QGYECGILLEKFNDLKEGDIFEAYTYEEISPDV

Sequences:

>Translated_673_residues
MEKIRVYQLAKEIGLESKELVTTLQELDISVKNHMSTLSEEEADTVRELYTESSAKDEDNKDETSQQQDEKQEDKKEQGT
TTDKPLVAPPITVGELAEQIGVESTSIITKLISKGVMANINQNLEEDSLEYLAQEFDFVVTQEEPEEDEKSEEDRIIEQI
RSEEPKGEPLERAPVVTVMGHVDHGKTSILDSIRKTKSIEKEAGGITQHIGASRVLYNDKTVVFLDTPGHEAFTEMRARG
AHVTDIAILVVAADDGVMPQTVEAINHAKSAGVPIIVAINKTDKPDANPDRVKQELTEYNLVTEEWGGDTICVSVSAVQN
EGIDELLEMVQLVAEMNELTSYPQNTGKGTVIEAKLDKGRGPVATVLVEDGTLKVGDSVLCGSTFGNIRAMVDDRGKRLK
QANPVTPVEILGLENIPEAGDEFQVVPDEKMAREIAKKKQEKEREEKLKRSQSVSLDNLFDQIKEGEQKELNIIVKGDVR
GSVEALRESLLKLNDNEHEIKVNVIHAGVGTVSESDIMLAVASNAIIIGFNVRPDPNARKAAEREQIDMRVYRVIYEAIE
DVKAAMAGLLDPEYKEVVQGQIEVRQLFKVSRIGTIAGCYVTDGYIRNNSYIRVIRDGRVIHEGNIKNLKRFQDDVKEVQ
QGYECGILLEKFNDLKEGDIFEAYTYEEISPDV
>Mature_673_residues
MEKIRVYQLAKEIGLESKELVTTLQELDISVKNHMSTLSEEEADTVRELYTESSAKDEDNKDETSQQQDEKQEDKKEQGT
TTDKPLVAPPITVGELAEQIGVESTSIITKLISKGVMANINQNLEEDSLEYLAQEFDFVVTQEEPEEDEKSEEDRIIEQI
RSEEPKGEPLERAPVVTVMGHVDHGKTSILDSIRKTKSIEKEAGGITQHIGASRVLYNDKTVVFLDTPGHEAFTEMRARG
AHVTDIAILVVAADDGVMPQTVEAINHAKSAGVPIIVAINKTDKPDANPDRVKQELTEYNLVTEEWGGDTICVSVSAVQN
EGIDELLEMVQLVAEMNELTSYPQNTGKGTVIEAKLDKGRGPVATVLVEDGTLKVGDSVLCGSTFGNIRAMVDDRGKRLK
QANPVTPVEILGLENIPEAGDEFQVVPDEKMAREIAKKKQEKEREEKLKRSQSVSLDNLFDQIKEGEQKELNIIVKGDVR
GSVEALRESLLKLNDNEHEIKVNVIHAGVGTVSESDIMLAVASNAIIIGFNVRPDPNARKAAEREQIDMRVYRVIYEAIE
DVKAAMAGLLDPEYKEVVQGQIEVRQLFKVSRIGTIAGCYVTDGYIRNNSYIRVIRDGRVIHEGNIKNLKRFQDDVKEVQ
QGYECGILLEKFNDLKEGDIFEAYTYEEISPDV

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI53729339, Length=550, Percent_Identity=38.5454545454545, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI53729337, Length=550, Percent_Identity=38.5454545454545, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI84043963, Length=487, Percent_Identity=29.1581108829569, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI157426893, Length=237, Percent_Identity=27.0042194092827, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1789559, Length=585, Percent_Identity=52.4786324786325, Blast_Score=586, Evalue=1e-168,
Organism=Escherichia coli, GI2367247, Length=214, Percent_Identity=33.1775700934579, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI48994988, Length=140, Percent_Identity=34.2857142857143, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1790412, Length=235, Percent_Identity=33.1914893617021, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1789737, Length=235, Percent_Identity=33.1914893617021, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1788922, Length=231, Percent_Identity=28.1385281385281, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994658, Length=545, Percent_Identity=34.8623853211009, Blast_Score=277, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI212656558, Length=566, Percent_Identity=25.9717314487633, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17557151, Length=217, Percent_Identity=26.7281105990783, Blast_Score=80, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=230, Percent_Identity=32.6086956521739, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25141371, Length=229, Percent_Identity=30.1310043668122, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324550, Length=644, Percent_Identity=34.9378881987578, Blast_Score=348, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6319282, Length=549, Percent_Identity=26.5938069216758, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323320, Length=237, Percent_Identity=27.8481012658228, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=232, Percent_Identity=31.8965517241379, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28572034, Length=572, Percent_Identity=39.3356643356643, Blast_Score=360, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24656849, Length=554, Percent_Identity=26.5342960288809, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI160714833, Length=107, Percent_Identity=54.2056074766355, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI78706572, Length=256, Percent_Identity=27.734375, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281363316, Length=226, Percent_Identity=33.1858407079646, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17864358, Length=226, Percent_Identity=33.1858407079646, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921738, Length=131, Percent_Identity=40.4580152671756, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582462, Length=150, Percent_Identity=31.3333333333333, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19922690, Length=176, Percent_Identity=30.6818181818182, Blast_Score=66, Evalue=1e-10,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2_NATTJ (B2A397)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917615.1
- ProteinModelPortal:   B2A397
- SMR:   B2A397
- GeneID:   6314867
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1444
- HOGENOM:   HBG635083
- OMA:   QYGRIRS
- GO:   GO:0005737
- HAMAP:   MF_00100_B
- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- TIGRFAMs:   TIGR00487
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 74858; Mature: 74858

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIRVYQLAKEIGLESKELVTTLQELDISVKNHMSTLSEEEADTVRELYTESSAKDEDN
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KDETSQQQDEKQEDKKEQGTTTDKPLVAPPITVGELAEQIGVESTSIITKLISKGVMANI
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHH
NQNLEEDSLEYLAQEFDFVVTQEEPEEDEKSEEDRIIEQIRSEEPKGEPLERAPVVTVMG
CCCCCHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
HVDHGKTSILDSIRKTKSIEKEAGGITQHIGASRVLYNDKTVVFLDTPGHEAFTEMRARG
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCEEEEEECCCHHHHHHHHHCC
AHVTDIAILVVAADDGVMPQTVEAINHAKSAGVPIIVAINKTDKPDANPDRVKQELTEYN
CCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHC
LVTEEWGGDTICVSVSAVQNEGIDELLEMVQLVAEMNELTSYPQNTGKGTVIEAKLDKGR
CCHHHCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
GPVATVLVEDGTLKVGDSVLCGSTFGNIRAMVDDRGKRLKQANPVTPVEILGLENIPEAG
CCEEEEEEECCCEEECCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
DEFQVVPDEKMAREIAKKKQEKEREEKLKRSQSVSLDNLFDQIKEGEQKELNIIVKGDVR
CCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCC
GSVEALRESLLKLNDNEHEIKVNVIHAGVGTVSESDIMLAVASNAIIIGFNVRPDPNARK
CHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCEEEEEEECCCCCCHHH
AAEREQIDMRVYRVIYEAIEDVKAAMAGLLDPEYKEVVQGQIEVRQLFKVSRIGTIAGCY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
VTDGYIRNNSYIRVIRDGRVIHEGNIKNLKRFQDDVKEVQQGYECGILLEKFNDLKEGDI
EECCEEECCCEEEEEECCEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCC
FEAYTYEEISPDV
EEEEECCCCCCCC
>Mature Secondary Structure
MEKIRVYQLAKEIGLESKELVTTLQELDISVKNHMSTLSEEEADTVRELYTESSAKDEDN
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KDETSQQQDEKQEDKKEQGTTTDKPLVAPPITVGELAEQIGVESTSIITKLISKGVMANI
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHH
NQNLEEDSLEYLAQEFDFVVTQEEPEEDEKSEEDRIIEQIRSEEPKGEPLERAPVVTVMG
CCCCCHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
HVDHGKTSILDSIRKTKSIEKEAGGITQHIGASRVLYNDKTVVFLDTPGHEAFTEMRARG
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCEEEEEECCCHHHHHHHHHCC
AHVTDIAILVVAADDGVMPQTVEAINHAKSAGVPIIVAINKTDKPDANPDRVKQELTEYN
CCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHC
LVTEEWGGDTICVSVSAVQNEGIDELLEMVQLVAEMNELTSYPQNTGKGTVIEAKLDKGR
CCHHHCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
GPVATVLVEDGTLKVGDSVLCGSTFGNIRAMVDDRGKRLKQANPVTPVEILGLENIPEAG
CCEEEEEEECCCEEECCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
DEFQVVPDEKMAREIAKKKQEKEREEKLKRSQSVSLDNLFDQIKEGEQKELNIIVKGDVR
CCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCC
GSVEALRESLLKLNDNEHEIKVNVIHAGVGTVSESDIMLAVASNAIIIGFNVRPDPNARK
CHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCEEEEEEECCCCCCHHH
AAEREQIDMRVYRVIYEAIEDVKAAMAGLLDPEYKEVVQGQIEVRQLFKVSRIGTIAGCY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
VTDGYIRNNSYIRVIRDGRVIHEGNIKNLKRFQDDVKEVQQGYECGILLEKFNDLKEGDI
EECCEEECCCEEEEEECCEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCC
FEAYTYEEISPDV
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA