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The map label for this gene is asnB [H]

Identifier: 188586005

GI number: 188586005

Start: 1448910

End: 1450772

Strand: Direct

Name: asnB [H]

Synonym: Nther_1379

Alternate gene names: 188586005

Gene position: 1448910-1450772 (Clockwise)

Preceding gene: 188586003

Following gene: 188586006

Centisome position: 45.77

GC content: 32.8

Gene sequence:

>1863_bases
ATGAGTGGTTTGGCTGGTGTTATGTCGCCTAACAGAGAATTTGAAAGACAGAAAATTGTTGATAACATGCTGGCAACACT
TAATCACAGGGGACCAGGGATCAGAAAGAAACATTCTATTAATAAAATTACTATGGGAATGACCGTGAACGTTGAGAAAG
AACAGCAGGTTTTGCCTTATGTAAATGAATATAACAACAAAATGATGATTTGTGATGCCACTATCTTCAATTCACATGAA
CTGGGACAACAATTAAAAGCTAAGGGACATTATTTAAGGACCGGTAATGGAGAAGAAATTTTGCTTCATCTCTATGAAGA
ATGGGGTTTGGATGGATTACAAAAGGTCAACGGATTTTTTAGTCTGGTGATTTATGACAAAAATACAGATAAGTTAATTG
CCGCACGTGATCCCTTTGGCGTTAAACCTCTTTATTATCATCAAAAAAATGGTACCATACTCTTTTCTTCTGAATTAAAG
GGAATAGTAGCTTCTAATTTAATTTCTCCTAAATTAAACTCCTATTGCCTCAATTTGTATCTAATATTACAGTACCCTCC
GGAGCCTTACAGTATGATTGATGATGTCTACCGTGTCCTTCCAGGAACTGCAATGACTTTTAATAAAGCTAGCATAGAAT
CTACTTACAAATATCATACTTTTGTATTTAATGGTTCGAATAAATTCACTCAATCAAAACCAAAGGGAGGTAATAAGCAA
GAAATAGCAGAACAAACCAAAACTTTATTTCAGAAAAGTATTCAGGAACAATTTGAAAACACATCTAATCCAGGAGCATT
TCTTTCAAGTGGAATAGATTCTTCCTCAGTAGTTGCTCTGTTAAATAAAAAATATGTTCCGGGGATTTCTACATTTTCCG
TGGGGTTTCATTTTCAAAAGTATGATGAACTTGAATTTATTAAAAATTTAACAGCTCAACTTCAGGTTAACAATTATCAA
AAATTGGTGTCTTTAGAGGAATTGAAGAACGAATTGCGTAAATTTGTGTGGCATCTTGACGAACCAGTAGCTGATCCTTC
TGCCTTTGGTTTATATAAAGGGGCTGAACTTGCAAGTAATTTTGTCAAAGAAATTTTTTCAGGAGAAGGAGCAGATGAAT
TTTTTGGTGGATACAATATCTACCAAGAACCCTTATTTGTCAACAAAATTCAAAAATTACCATCGACTGTATTATCTACT
ATGAATAGTATATTTAATATTATTCCTTACAATATAAAAGGAAAGAGTTTATTTAAAAGAGCCAACACTCCTCTATCCGA
GAGATATTTCGGTAATGCCATGATTTTCACTCCAGCTGAAAAGGATAACTTAATTTCCAAAGAACATCAAATTTCTGGAG
TCTTAGACAAAATGTTTAAACCTATCTATAAAAATGTAGAAAAATGCCACGATGTTAATAAAATGCAGTATGTAGACATA
AATTCCTGGATGACAGGTGATATAATGCTAAAAGTTGATAGAATGTTAATGGCACATTCACTGAGCTTAAGAGCCCCTTT
TCTTAATCTTGAATTGACAGACTGGGCTTATAATTTACCTGTTAAGTGGAAAGTTTCCCAAAAAGAGACCAAAATAGCTT
TTAGAAGAGCCATGAATGACATTTTACCTCAACAAAGTGTTGTTAAGAAAAAACTAGGGTTTCCAGTACCTACTGATAAA
TGGTTTGAAAATTCTTTGATTACATGGGCTAAAGATTTGTTATTGTCTAATGAAAGTAAAAAATACTTTAATCCAAACAA
AGTTAAAAAACTAATGAAACCCGATAAAAAAACTGATTTTCAGGGCAGAAAATTGTGGACACTATTGATATTTTTACTAT
GGCAAGATATGTATCTAAAATAA

Upstream 100 bases:

>100_bases
CCGATATTTCTTAAATATACTAACATGGAAAGGGATAAAACTCAAGGTTATTTTAAATAAATTAACCTTCAAATAATATT
ATGACTGGAGGGCAGTCTTT

Downstream 100 bases:

>100_bases
ACTGGCAAGAATTGACTCTAATATATTAAATAGTGTATACTAAACACAAAATTAAGACCTTAGTACTAACTTTAGAAGTT
TAAATTACCGACAGGAGTTG

Product: Asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 620; Mature: 619

Protein sequence:

>620_residues
MSGLAGVMSPNREFERQKIVDNMLATLNHRGPGIRKKHSINKITMGMTVNVEKEQQVLPYVNEYNNKMMICDATIFNSHE
LGQQLKAKGHYLRTGNGEEILLHLYEEWGLDGLQKVNGFFSLVIYDKNTDKLIAARDPFGVKPLYYHQKNGTILFSSELK
GIVASNLISPKLNSYCLNLYLILQYPPEPYSMIDDVYRVLPGTAMTFNKASIESTYKYHTFVFNGSNKFTQSKPKGGNKQ
EIAEQTKTLFQKSIQEQFENTSNPGAFLSSGIDSSSVVALLNKKYVPGISTFSVGFHFQKYDELEFIKNLTAQLQVNNYQ
KLVSLEELKNELRKFVWHLDEPVADPSAFGLYKGAELASNFVKEIFSGEGADEFFGGYNIYQEPLFVNKIQKLPSTVLST
MNSIFNIIPYNIKGKSLFKRANTPLSERYFGNAMIFTPAEKDNLISKEHQISGVLDKMFKPIYKNVEKCHDVNKMQYVDI
NSWMTGDIMLKVDRMLMAHSLSLRAPFLNLELTDWAYNLPVKWKVSQKETKIAFRRAMNDILPQQSVVKKKLGFPVPTDK
WFENSLITWAKDLLLSNESKKYFNPNKVKKLMKPDKKTDFQGRKLWTLLIFLLWQDMYLK

Sequences:

>Translated_620_residues
MSGLAGVMSPNREFERQKIVDNMLATLNHRGPGIRKKHSINKITMGMTVNVEKEQQVLPYVNEYNNKMMICDATIFNSHE
LGQQLKAKGHYLRTGNGEEILLHLYEEWGLDGLQKVNGFFSLVIYDKNTDKLIAARDPFGVKPLYYHQKNGTILFSSELK
GIVASNLISPKLNSYCLNLYLILQYPPEPYSMIDDVYRVLPGTAMTFNKASIESTYKYHTFVFNGSNKFTQSKPKGGNKQ
EIAEQTKTLFQKSIQEQFENTSNPGAFLSSGIDSSSVVALLNKKYVPGISTFSVGFHFQKYDELEFIKNLTAQLQVNNYQ
KLVSLEELKNELRKFVWHLDEPVADPSAFGLYKGAELASNFVKEIFSGEGADEFFGGYNIYQEPLFVNKIQKLPSTVLST
MNSIFNIIPYNIKGKSLFKRANTPLSERYFGNAMIFTPAEKDNLISKEHQISGVLDKMFKPIYKNVEKCHDVNKMQYVDI
NSWMTGDIMLKVDRMLMAHSLSLRAPFLNLELTDWAYNLPVKWKVSQKETKIAFRRAMNDILPQQSVVKKKLGFPVPTDK
WFENSLITWAKDLLLSNESKKYFNPNKVKKLMKPDKKTDFQGRKLWTLLIFLLWQDMYLK
>Mature_619_residues
SGLAGVMSPNREFERQKIVDNMLATLNHRGPGIRKKHSINKITMGMTVNVEKEQQVLPYVNEYNNKMMICDATIFNSHEL
GQQLKAKGHYLRTGNGEEILLHLYEEWGLDGLQKVNGFFSLVIYDKNTDKLIAARDPFGVKPLYYHQKNGTILFSSELKG
IVASNLISPKLNSYCLNLYLILQYPPEPYSMIDDVYRVLPGTAMTFNKASIESTYKYHTFVFNGSNKFTQSKPKGGNKQE
IAEQTKTLFQKSIQEQFENTSNPGAFLSSGIDSSSVVALLNKKYVPGISTFSVGFHFQKYDELEFIKNLTAQLQVNNYQK
LVSLEELKNELRKFVWHLDEPVADPSAFGLYKGAELASNFVKEIFSGEGADEFFGGYNIYQEPLFVNKIQKLPSTVLSTM
NSIFNIIPYNIKGKSLFKRANTPLSERYFGNAMIFTPAEKDNLISKEHQISGVLDKMFKPIYKNVEKCHDVNKMQYVDIN
SWMTGDIMLKVDRMLMAHSLSLRAPFLNLELTDWAYNLPVKWKVSQKETKIAFRRAMNDILPQQSVVKKKLGFPVPTDKW
FENSLITWAKDLLLSNESKKYFNPNKVKKLMKPDKKTDFQGRKLWTLLIFLLWQDMYLK

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Drosophila melanogaster, GI45553209, Length=377, Percent_Identity=25.1989389920424, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71208; Mature: 71077

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLAGVMSPNREFERQKIVDNMLATLNHRGPGIRKKHSINKITMGMTVNVEKEQQVLPY
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCHHHHCCH
VNEYNNKMMICDATIFNSHELGQQLKAKGHYLRTGNGEEILLHLYEEWGLDGLQKVNGFF
HHHCCCEEEEEEEEECCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCHHHHHHCCEE
SLVIYDKNTDKLIAARDPFGVKPLYYHQKNGTILFSSELKGIVASNLISPKLNSYCLNLY
EEEEEECCCCCEEEECCCCCCCCEEEECCCCEEEECCCCCHHHHHHHCCCCHHHEEEEEE
LILQYPPEPYSMIDDVYRVLPGTAMTFNKASIESTYKYHTFVFNGSNKFTQSKPKGGNKQ
EEEECCCCHHHHHHHHHHHCCCCEEEECCHHHCCCEEEEEEEECCCCCCCCCCCCCCCHH
EIAEQTKTLFQKSIQEQFENTSNPGAFLSSGIDSSSVVALLNKKYVPGISTFSVGFHFQK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCEEEECCEEHH
YDELEFIKNLTAQLQVNNYQKLVSLEELKNELRKFVWHLDEPVADPSAFGLYKGAELASN
HHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHH
FVKEIFSGEGADEFFGGYNIYQEPLFVNKIQKLPSTVLSTMNSIFNIIPYNIKGKSLFKR
HHHHHHCCCCCHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
ANTPLSERYFGNAMIFTPAEKDNLISKEHQISGVLDKMFKPIYKNVEKCHDVNKMQYVDI
CCCCHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NSWMTGDIMLKVDRMLMAHSLSLRAPFLNLELTDWAYNLPVKWKVSQKETKIAFRRAMND
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECEEECCCEEEEECHHHHHHHHHHHHHH
ILPQQSVVKKKLGFPVPTDKWFENSLITWAKDLLLSNESKKYFNPNKVKKLMKPDKKTDF
HCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCC
QGRKLWTLLIFLLWQDMYLK
CCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGLAGVMSPNREFERQKIVDNMLATLNHRGPGIRKKHSINKITMGMTVNVEKEQQVLPY
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCHHHHCCH
VNEYNNKMMICDATIFNSHELGQQLKAKGHYLRTGNGEEILLHLYEEWGLDGLQKVNGFF
HHHCCCEEEEEEEEECCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCHHHHHHCCEE
SLVIYDKNTDKLIAARDPFGVKPLYYHQKNGTILFSSELKGIVASNLISPKLNSYCLNLY
EEEEEECCCCCEEEECCCCCCCCEEEECCCCEEEECCCCCHHHHHHHCCCCHHHEEEEEE
LILQYPPEPYSMIDDVYRVLPGTAMTFNKASIESTYKYHTFVFNGSNKFTQSKPKGGNKQ
EEEECCCCHHHHHHHHHHHCCCCEEEECCHHHCCCEEEEEEEECCCCCCCCCCCCCCCHH
EIAEQTKTLFQKSIQEQFENTSNPGAFLSSGIDSSSVVALLNKKYVPGISTFSVGFHFQK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCEEEECCEEHH
YDELEFIKNLTAQLQVNNYQKLVSLEELKNELRKFVWHLDEPVADPSAFGLYKGAELASN
HHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHH
FVKEIFSGEGADEFFGGYNIYQEPLFVNKIQKLPSTVLSTMNSIFNIIPYNIKGKSLFKR
HHHHHHCCCCCHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
ANTPLSERYFGNAMIFTPAEKDNLISKEHQISGVLDKMFKPIYKNVEKCHDVNKMQYVDI
CCCCHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NSWMTGDIMLKVDRMLMAHSLSLRAPFLNLELTDWAYNLPVKWKVSQKETKIAFRRAMND
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECEEECCCEEEEECHHHHHHHHHHHHHH
ILPQQSVVKKKLGFPVPTDKWFENSLITWAKDLLLSNESKKYFNPNKVKKLMKPDKKTDF
HCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCC
QGRKLWTLLIFLLWQDMYLK
CCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]