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The map label for this gene is relA [H]

Identifier: 188585914

GI number: 188585914

Start: 1358998

End: 1361187

Strand: Direct

Name: relA [H]

Synonym: Nther_1288

Alternate gene names: 188585914

Gene position: 1358998-1361187 (Clockwise)

Preceding gene: 188585913

Following gene: 188585915

Centisome position: 42.93

GC content: 35.89

Gene sequence:

>2190_bases
TTGTTAGATGCCGAACAGTTGTTTTTCAGTTTAAAAGAAAAAATAAAATCATACTACCCTCTTGACACAGATTTAGAACA
ACTGGATAGAGCCTTTGAGTTTGCCAGGAGCTATCATAATGATCAAAAACGAGTTTCAGGTGAGGAATATATAGTTCATC
CCATTGAAGTTGCCCTAGTGCTAGCGGATTTAGAGTTGGATCTAGATACCATTAGTGCAGGTTTACTGCACGATGTCATG
GAGGACACTCCTGCTACTAGAGAAGAGTTGGAGGAACTGTTCGGTGATGAAATTACTTTGCTAGTAGATGGGGTAACAAA
GCTTAGCAGATTGAATTTTAAAACTAAAGAAGAACATCAGGCAGAAAATTTAAGAAAAATGTTCTTTGCCATGGCAAATG
ATATTAGAGTTATTCTAATAAAATTAGCTGATAGAACTCATAATATGAGAACTCTAAATTATCTTCCAGAACGAAAACAA
AAAGAAATTGCTAAAGAAACTATGGAAATTTATGCTCCCCTTGCCAATCGTTTAGGTATATATAAAATTAAATGGGAATT
GGAAGACCTGGCATTTCGATATATAGAACCTGAACAGTATTATTATTTAGCGGATAGAATTGTACAGAAGAGAATGGAAA
GAGAAGAATATATTAAAAAAATAAAGGTTGACCTTCGGGATAAATTAAAAGAAATGGGCATAGATGGTGAGATTCATGGA
AGACCCAAGCATTTGTATAGTATTTATTCTAAAATGAAAAAAACAGGAAAAGATCTTAATGAAATCTATGATTTAATCGC
CCTAAGGATTATCGTTAATAATGTAAAAGATTGCTATGCAGTATTAGGAACTGTCCATACTATGTGGAAGCCCGTACCTG
GTCGTTTTAAGGATTATATTGCAGTTCCCAAGTCTAATATGTATCAATCTTTGCATACAACGGTGTTATGTCCACAAGGG
AATCTGGTAGAAATACAAATCAGGACATGGGATATGCATAAAACAGCAGAGTATGGTATTGCAGCTCACTGGAAATACAA
AGAAAATACGGACGACAAGAAGTTTGCTGAAAAAGTGTCCTGGTTTAGACAGTTGCTAGAATGGCAGCAAGAAACCAGTG
ATGCCTTAGAGTTTATGGAGCACTTAAAAGTTGATCTTTTCTCCGATGAAGTGTTTGTCTTTACTCCTAAAGGTGATGTG
ATTGACTTGCCCAGCGGCTCTACACCTATTGATTTTGCTTATAGGATTCATACCGATATAGGTAATACTTGTATTGGAGC
TAAAATAAGCGGTAGAATGGTCTCTCTAGATTATCAGTTACAAACGGGGGATGTAGTTGAAATTTTAACTTCTAAGCATG
CCACCCCGAGTCGTGACTGGCTAAACTTGGTTAAAAGCTCTCAGGCTAAAAATAAGATTAAACAATGGTTTAAACGTCAA
AGCAGAGATGAAAATATTCAACGAGGAAGAGAAATGATTGAAAGGGAAGCCAAGCGCTTAAAGATCTTTCCTGAGAAGTA
TATTAAAGATGAACAAGTTCAAGAAGAAGTGGCTGAAAAGTTTAATATGAAGAGTTATGATGATGTTTTAGCCGCTTTAG
GTTATGGTGGTATTACAGTAAATCAAGTTATTGGAAAATACCAGAGTATTTATAAAGCAAATCGGGAAGAGTTAGAAGAA
GAGGAAGATCAGGTAGAGGACTTCTCCAAGTATCTAAAAGAAGATCAAAGTCTCAAAAAAACCAAAGACAGTATAAGCGT
ACAGGGTCTTGAAAATATTGCCACTAGATTTGCAAAATGTTGCCATCCTTTACCAGGAGAACATATTGTAGGTTTTATAA
CACGAACTAGAGGTATCACTATCCATCGCCATGACTGTCCGAATGCTCAACATAAGCTTAAGAATAATGAACGCACTGTA
CCAGTTCACTGGGATGAAAGTGGAGATAATGACTTTCAAGTTAGCGTTTCCGTTTTTGCTGTAGATCGCCAAAGAATAGT
TCAAGATATTATGAAAGCCTTGGGCGATTCTAAAATTGCCGTGACTGCTATAGAAGCTAAGACGGACAAAAATCAAGTTG
CAAAAATCAATTTAACTGTATTAATCAAGAATCACTCACACTTACAATTTGTGATTGACAAACTACAGAATATTCAGGAT
GTATTGAAAGTACAAAGGGATAACCCCTAG

Upstream 100 bases:

>100_bases
TTATTCCAAATTATACATGTTATTATATAATCATAATATTTCGCCCGAGTATATTAAAGAGTTAGGTACTTACTAATATA
AAGGTGGGGAGATGAAAACA

Downstream 100 bases:

>100_bases
ATTAAGTGGCAGTGGTTTTATGAAAGGAGAGATACCATGAGAGCAGTGGTTCAAAGAGTTAGCAAATCTTACGTTAATGT
TAATGGAGAAAAAGTCGGAG

Product: (p)ppGpp synthetase I, SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 729; Mature: 729

Protein sequence:

>729_residues
MLDAEQLFFSLKEKIKSYYPLDTDLEQLDRAFEFARSYHNDQKRVSGEEYIVHPIEVALVLADLELDLDTISAGLLHDVM
EDTPATREELEELFGDEITLLVDGVTKLSRLNFKTKEEHQAENLRKMFFAMANDIRVILIKLADRTHNMRTLNYLPERKQ
KEIAKETMEIYAPLANRLGIYKIKWELEDLAFRYIEPEQYYYLADRIVQKRMEREEYIKKIKVDLRDKLKEMGIDGEIHG
RPKHLYSIYSKMKKTGKDLNEIYDLIALRIIVNNVKDCYAVLGTVHTMWKPVPGRFKDYIAVPKSNMYQSLHTTVLCPQG
NLVEIQIRTWDMHKTAEYGIAAHWKYKENTDDKKFAEKVSWFRQLLEWQQETSDALEFMEHLKVDLFSDEVFVFTPKGDV
IDLPSGSTPIDFAYRIHTDIGNTCIGAKISGRMVSLDYQLQTGDVVEILTSKHATPSRDWLNLVKSSQAKNKIKQWFKRQ
SRDENIQRGREMIEREAKRLKIFPEKYIKDEQVQEEVAEKFNMKSYDDVLAALGYGGITVNQVIGKYQSIYKANREELEE
EEDQVEDFSKYLKEDQSLKKTKDSISVQGLENIATRFAKCCHPLPGEHIVGFITRTRGITIHRHDCPNAQHKLKNNERTV
PVHWDESGDNDFQVSVSVFAVDRQRIVQDIMKALGDSKIAVTAIEAKTDKNQVAKINLTVLIKNHSHLQFVIDKLQNIQD
VLKVQRDNP

Sequences:

>Translated_729_residues
MLDAEQLFFSLKEKIKSYYPLDTDLEQLDRAFEFARSYHNDQKRVSGEEYIVHPIEVALVLADLELDLDTISAGLLHDVM
EDTPATREELEELFGDEITLLVDGVTKLSRLNFKTKEEHQAENLRKMFFAMANDIRVILIKLADRTHNMRTLNYLPERKQ
KEIAKETMEIYAPLANRLGIYKIKWELEDLAFRYIEPEQYYYLADRIVQKRMEREEYIKKIKVDLRDKLKEMGIDGEIHG
RPKHLYSIYSKMKKTGKDLNEIYDLIALRIIVNNVKDCYAVLGTVHTMWKPVPGRFKDYIAVPKSNMYQSLHTTVLCPQG
NLVEIQIRTWDMHKTAEYGIAAHWKYKENTDDKKFAEKVSWFRQLLEWQQETSDALEFMEHLKVDLFSDEVFVFTPKGDV
IDLPSGSTPIDFAYRIHTDIGNTCIGAKISGRMVSLDYQLQTGDVVEILTSKHATPSRDWLNLVKSSQAKNKIKQWFKRQ
SRDENIQRGREMIEREAKRLKIFPEKYIKDEQVQEEVAEKFNMKSYDDVLAALGYGGITVNQVIGKYQSIYKANREELEE
EEDQVEDFSKYLKEDQSLKKTKDSISVQGLENIATRFAKCCHPLPGEHIVGFITRTRGITIHRHDCPNAQHKLKNNERTV
PVHWDESGDNDFQVSVSVFAVDRQRIVQDIMKALGDSKIAVTAIEAKTDKNQVAKINLTVLIKNHSHLQFVIDKLQNIQD
VLKVQRDNP
>Mature_729_residues
MLDAEQLFFSLKEKIKSYYPLDTDLEQLDRAFEFARSYHNDQKRVSGEEYIVHPIEVALVLADLELDLDTISAGLLHDVM
EDTPATREELEELFGDEITLLVDGVTKLSRLNFKTKEEHQAENLRKMFFAMANDIRVILIKLADRTHNMRTLNYLPERKQ
KEIAKETMEIYAPLANRLGIYKIKWELEDLAFRYIEPEQYYYLADRIVQKRMEREEYIKKIKVDLRDKLKEMGIDGEIHG
RPKHLYSIYSKMKKTGKDLNEIYDLIALRIIVNNVKDCYAVLGTVHTMWKPVPGRFKDYIAVPKSNMYQSLHTTVLCPQG
NLVEIQIRTWDMHKTAEYGIAAHWKYKENTDDKKFAEKVSWFRQLLEWQQETSDALEFMEHLKVDLFSDEVFVFTPKGDV
IDLPSGSTPIDFAYRIHTDIGNTCIGAKISGRMVSLDYQLQTGDVVEILTSKHATPSRDWLNLVKSSQAKNKIKQWFKRQ
SRDENIQRGREMIEREAKRLKIFPEKYIKDEQVQEEVAEKFNMKSYDDVLAALGYGGITVNQVIGKYQSIYKANREELEE
EEDQVEDFSKYLKEDQSLKKTKDSISVQGLENIATRFAKCCHPLPGEHIVGFITRTRGITIHRHDCPNAQHKLKNNERTV
PVHWDESGDNDFQVSVSVFAVDRQRIVQDIMKALGDSKIAVTAIEAKTDKNQVAKINLTVLIKNHSHLQFVIDKLQNIQD
VLKVQRDNP

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=723, Percent_Identity=40.1106500691563, Blast_Score=576, Evalue=1e-165,
Organism=Escherichia coli, GI1789147, Length=689, Percent_Identity=40.2031930333817, Blast_Score=551, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 84836; Mature: 84836

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAEQLFFSLKEKIKSYYPLDTDLEQLDRAFEFARSYHNDQKRVSGEEYIVHPIEVALV
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEHHHHHHH
LADLELDLDTISAGLLHDVMEDTPATREELEELFGDEITLLVDGVTKLSRLNFKTKEEHQ
HHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCHHHH
AENLRKMFFAMANDIRVILIKLADRTHNMRTLNYLPERKQKEIAKETMEIYAPLANRLGI
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCE
YKIKWELEDLAFRYIEPEQYYYLADRIVQKRMEREEYIKKIKVDLRDKLKEMGIDGEIHG
EEEEEEHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RPKHLYSIYSKMKKTGKDLNEIYDLIALRIIVNNVKDCYAVLGTVHTMWKPVPGRFKDYI
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHE
AVPKSNMYQSLHTTVLCPQGNLVEIQIRTWDMHKTAEYGIAAHWKYKENTDDKKFAEKVS
ECCCHHHHHHHHHEEECCCCCEEEEEEEEECCCHHHCCCCEEEEEECCCCCHHHHHHHHH
WFRQLLEWQQETSDALEFMEHLKVDLFSDEVFVFTPKGDVIDLPSGSTPIDFAYRIHTDI
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEECCCCCEEECCCCCCCCEEEEEEECCC
GNTCIGAKISGRMVSLDYQLQTGDVVEILTSKHATPSRDWLNLVKSSQAKNKIKQWFKRQ
CCCEECCEECCEEEEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
SRDENIQRGREMIEREAKRLKIFPEKYIKDEQVQEEVAEKFNMKSYDDVLAALGYGGITV
CCCHHHHHHHHHHHHHHHHEECCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCH
NQVIGKYQSIYKANREELEEEEDQVEDFSKYLKEDQSLKKTKDSISVQGLENIATRFAKC
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CHPLPGEHIVGFITRTRGITIHRHDCPNAQHKLKNNERTVPVHWDESGDNDFQVSVSVFA
CCCCCCCHHEEHEECCCCEEEEECCCCCHHHHHCCCCCEEEEEECCCCCCCEEEEEEEEE
VDRQRIVQDIMKALGDSKIAVTAIEAKTDKNQVAKINLTVLIKNHSHLQFVIDKLQNIQD
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHH
VLKVQRDNP
HHHHHCCCC
>Mature Secondary Structure
MLDAEQLFFSLKEKIKSYYPLDTDLEQLDRAFEFARSYHNDQKRVSGEEYIVHPIEVALV
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEHHHHHHH
LADLELDLDTISAGLLHDVMEDTPATREELEELFGDEITLLVDGVTKLSRLNFKTKEEHQ
HHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCHHHH
AENLRKMFFAMANDIRVILIKLADRTHNMRTLNYLPERKQKEIAKETMEIYAPLANRLGI
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCE
YKIKWELEDLAFRYIEPEQYYYLADRIVQKRMEREEYIKKIKVDLRDKLKEMGIDGEIHG
EEEEEEHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RPKHLYSIYSKMKKTGKDLNEIYDLIALRIIVNNVKDCYAVLGTVHTMWKPVPGRFKDYI
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHE
AVPKSNMYQSLHTTVLCPQGNLVEIQIRTWDMHKTAEYGIAAHWKYKENTDDKKFAEKVS
ECCCHHHHHHHHHEEECCCCCEEEEEEEEECCCHHHCCCCEEEEEECCCCCHHHHHHHHH
WFRQLLEWQQETSDALEFMEHLKVDLFSDEVFVFTPKGDVIDLPSGSTPIDFAYRIHTDI
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEECCCCCEEECCCCCCCCEEEEEEECCC
GNTCIGAKISGRMVSLDYQLQTGDVVEILTSKHATPSRDWLNLVKSSQAKNKIKQWFKRQ
CCCEECCEECCEEEEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
SRDENIQRGREMIEREAKRLKIFPEKYIKDEQVQEEVAEKFNMKSYDDVLAALGYGGITV
CCCHHHHHHHHHHHHHHHHEECCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCH
NQVIGKYQSIYKANREELEEEEDQVEDFSKYLKEDQSLKKTKDSISVQGLENIATRFAKC
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CHPLPGEHIVGFITRTRGITIHRHDCPNAQHKLKNNERTVPVHWDESGDNDFQVSVSVFA
CCCCCCCHHEEHEECCCCEEEEECCCCCHHHHHCCCCCEEEEEECCCCCCCEEEEEEEEE
VDRQRIVQDIMKALGDSKIAVTAIEAKTDKNQVAKINLTVLIKNHSHLQFVIDKLQNIQD
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHH
VLKVQRDNP
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9383190; 9384377 [H]