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The map label for this gene is braB [H]

Identifier: 188585703

GI number: 188585703

Start: 1132489

End: 1133787

Strand: Reverse

Name: braB [H]

Synonym: Nther_1076

Alternate gene names: 188585703

Gene position: 1133787-1132489 (Counterclockwise)

Preceding gene: 188585706

Following gene: 188585700

Centisome position: 35.82

GC content: 38.34

Gene sequence:

>1299_bases
ATGAACAAAAAATATACTGATACAGTAGTGGTCGGTTTCGCACTCTTTGCCATGTTCTTGGGGGCAGGAAATGTAATTTT
TCCACCGTTTTTAGGTCATTCTTCTGGTACCGACTGGTTGCCTGCCTTGATCGGTTTTTTGTTAACTGGTGTTGGACTAC
CCTTACTTGGTATCATCGCTGTTGCTGTAGCTGGTGGCACTGTCGACCACTTAGCTAAAAATGTCAGTAAAAACTTCGGT
AAACTTTTCGGCTCAGTAATAGTTTTAACTATTGGCCCACTCTTGGCCATTCCACGAACAGGGGCAACAGCTTTTGAGTT
AGGGATAGCTCCCTTTCTTGAGGAAACAATAATTACTTCAATAATAGCAACAACAATATTTTTTGGAATAACATTATATT
TTTCTCTAAATCCATCAACAGTGATTGATCGGATTGGGAAGTATCTCACACCAACATTGGTTGCAGTATTAGGTATTATA
GTCATAGTTGGTTTTATCAGCCCAGTTGGAGCACCACAAGATGTGAGTTCTGAAAATACCTTTACCATAGGTTTTACCGA
AGGGTATCAAACTATGGATGCACTGGCTTCAATTCTTTTTGCCGGTATTGTTATTGGAAGCTTAATCGACCGGGGATATA
AAACTGTATCAGATCAGATCTCCTTGACAATTAGGGCGGGAGCTATTGCAGCCACTGGACTCGGGTTAATTTATGGAGGA
CTTGTGTACTTAGGAAGTACTGCTAGCGGAGTGTTTGATGCCGGAATTCACAGAACAGATTTATTAATTGGTATCAGTGG
TAATTTACTCGGCTCTCTTGGCACTGCTATTCTAGGAATAAGCGTTTTCTTAGCGTGCTTAACTACTTCTATTGGATTAA
CTACTACGGTAGGAAAATATTTCAATGATTTATCTGAAGGTAAATTAAAATATAACCATCTCGTGATCGGTACTGTACTA
TTTAGCCTATTCTTTGCTAATGCCGGTGTAGAATTAATTGTAACTATAGCAGAGCCAATTTTAATTACTATCTATCCAAC
AGCCATTGTATTGATTATTATGTCATTGTTTGATAAATACATTAATGACAAAATCGCCTATAGAGGGGCCGTTGTAGCAA
CCTTTTTAATTGGTTTGATGGATGCTTTTAGAACATTAGATTTGAACCCCAAAACTATAGAAATTTTTATGTCTAACCTC
CCCTTAGCCGAATACGGTATGCCTTGGATTACACCAGCAATTGTGTGCGCTATACTGGCTTCAGTTGCTAAAAAACTAAC
ATCTGAAAACATGTTTTAA

Upstream 100 bases:

>100_bases
ACTTTTAAGATCTATAGTAGTTATAAATAAATTAATTCATTTATTCGTCGAAATAAAGGAGAAAAAATAGCTTAAATATT
ATCCAGTAGGAGGGTCAGTA

Downstream 100 bases:

>100_bases
TTTATTTACTTCTTAAAAGTCTTTAAGTACCATGAATTATATTAATTATATTTAAGTATGGCAGATAACAAACAAGTACG
TGCCTTGGATATTTTCTTAG

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNKKYTDTVVVGFALFAMFLGAGNVIFPPFLGHSSGTDWLPALIGFLLTGVGLPLLGIIAVAVAGGTVDHLAKNVSKNFG
KLFGSVIVLTIGPLLAIPRTGATAFELGIAPFLEETIITSIIATTIFFGITLYFSLNPSTVIDRIGKYLTPTLVAVLGII
VIVGFISPVGAPQDVSSENTFTIGFTEGYQTMDALASILFAGIVIGSLIDRGYKTVSDQISLTIRAGAIAATGLGLIYGG
LVYLGSTASGVFDAGIHRTDLLIGISGNLLGSLGTAILGISVFLACLTTSIGLTTTVGKYFNDLSEGKLKYNHLVIGTVL
FSLFFANAGVELIVTIAEPILITIYPTAIVLIIMSLFDKYINDKIAYRGAVVATFLIGLMDAFRTLDLNPKTIEIFMSNL
PLAEYGMPWITPAIVCAILASVAKKLTSENMF

Sequences:

>Translated_432_residues
MNKKYTDTVVVGFALFAMFLGAGNVIFPPFLGHSSGTDWLPALIGFLLTGVGLPLLGIIAVAVAGGTVDHLAKNVSKNFG
KLFGSVIVLTIGPLLAIPRTGATAFELGIAPFLEETIITSIIATTIFFGITLYFSLNPSTVIDRIGKYLTPTLVAVLGII
VIVGFISPVGAPQDVSSENTFTIGFTEGYQTMDALASILFAGIVIGSLIDRGYKTVSDQISLTIRAGAIAATGLGLIYGG
LVYLGSTASGVFDAGIHRTDLLIGISGNLLGSLGTAILGISVFLACLTTSIGLTTTVGKYFNDLSEGKLKYNHLVIGTVL
FSLFFANAGVELIVTIAEPILITIYPTAIVLIIMSLFDKYINDKIAYRGAVVATFLIGLMDAFRTLDLNPKTIEIFMSNL
PLAEYGMPWITPAIVCAILASVAKKLTSENMF
>Mature_432_residues
MNKKYTDTVVVGFALFAMFLGAGNVIFPPFLGHSSGTDWLPALIGFLLTGVGLPLLGIIAVAVAGGTVDHLAKNVSKNFG
KLFGSVIVLTIGPLLAIPRTGATAFELGIAPFLEETIITSIIATTIFFGITLYFSLNPSTVIDRIGKYLTPTLVAVLGII
VIVGFISPVGAPQDVSSENTFTIGFTEGYQTMDALASILFAGIVIGSLIDRGYKTVSDQISLTIRAGAIAATGLGLIYGG
LVYLGSTASGVFDAGIHRTDLLIGISGNLLGSLGTAILGISVFLACLTTSIGLTTTVGKYFNDLSEGKLKYNHLVIGTVL
FSLFFANAGVELIVTIAEPILITIYPTAIVLIIMSLFDKYINDKIAYRGAVVATFLIGLMDAFRTLDLNPKTIEIFMSNL
PLAEYGMPWITPAIVCAILASVAKKLTSENMF

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=432, Percent_Identity=31.25, Blast_Score=223, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 45487; Mature: 45487

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKYTDTVVVGFALFAMFLGAGNVIFPPFLGHSSGTDWLPALIGFLLTGVGLPLLGIIA
CCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAVAGGTVDHLAKNVSKNFGKLFGSVIVLTIGPLLAIPRTGATAFELGIAPFLEETIITS
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
IIATTIFFGITLYFSLNPSTVIDRIGKYLTPTLVAVLGIIVIVGFISPVGAPQDVSSENT
HHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
FTIGFTEGYQTMDALASILFAGIVIGSLIDRGYKTVSDQISLTIRAGAIAATGLGLIYGG
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHH
LVYLGSTASGVFDAGIHRTDLLIGISGNLLGSLGTAILGISVFLACLTTSIGLTTTVGKY
HHHHCCCCCCHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FNDLSEGKLKYNHLVIGTVLFSLFFANAGVELIVTIAEPILITIYPTAIVLIIMSLFDKY
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCEEEEHHHHHHHHHHHHHHHH
INDKIAYRGAVVATFLIGLMDAFRTLDLNPKTIEIFMSNLPLAEYGMPWITPAIVCAILA
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCHHHCCCCHHHHHHHHHHHH
SVAKKLTSENMF
HHHHHHHCCCCC
>Mature Secondary Structure
MNKKYTDTVVVGFALFAMFLGAGNVIFPPFLGHSSGTDWLPALIGFLLTGVGLPLLGIIA
CCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAVAGGTVDHLAKNVSKNFGKLFGSVIVLTIGPLLAIPRTGATAFELGIAPFLEETIITS
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
IIATTIFFGITLYFSLNPSTVIDRIGKYLTPTLVAVLGIIVIVGFISPVGAPQDVSSENT
HHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
FTIGFTEGYQTMDALASILFAGIVIGSLIDRGYKTVSDQISLTIRAGAIAATGLGLIYGG
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHH
LVYLGSTASGVFDAGIHRTDLLIGISGNLLGSLGTAILGISVFLACLTTSIGLTTTVGKY
HHHHCCCCCCHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FNDLSEGKLKYNHLVIGTVLFSLFFANAGVELIVTIAEPILITIYPTAIVLIIMSLFDKY
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCEEEEHHHHHHHHHHHHHHHH
INDKIAYRGAVVATFLIGLMDAFRTLDLNPKTIEIFMSNLPLAEYGMPWITPAIVCAILA
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCHHHCCCCHHHHHHHHHHHH
SVAKKLTSENMF
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]