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The map label for this gene is fadH [C]

Identifier: 188585649

GI number: 188585649

Start: 1090352

End: 1092289

Strand: Reverse

Name: fadH [C]

Synonym: Nther_1022

Alternate gene names: 188585649

Gene position: 1092289-1090352 (Counterclockwise)

Preceding gene: 188585651

Following gene: 188585648

Centisome position: 34.51

GC content: 39.01

Gene sequence:

>1938_bases
ATGGACAAGTATTCAAGATTATTTGAAGGTGTTAAGATAGGTGATATGAAATTAAGAAACCGACTTGTAATGTCACCTAT
GGGTACCTTCAACGAAAACTATGATGGAAGTATTTCACAAGAACAGATTGATTATTATGAAGCCAGAGCTAAAGGCGGAG
TTGGTATGATAATTGCCGAAGCTCAATACGTCACTAACAAAACCGACCCTTGGATTGAATCAATGACAGCTGTTGGTACT
GATATTCAAATGAAAGGCTGGGCACGTATGGCAGAAGCAGTACATGCTCATGGTGCTAAAATTTGTCTACAGTTAAGTTG
TGGTCTTGGAAGAAACGCTTTCCCCTTTGACTCAAGACAGATGGTCTCAGCTTCAGCAGTTCCTTCATTCTTCTTTCCAG
ATCAATTATGCCGTCCCTTAGAAAAAGACGAAATTAAAGACATTGTAGAATCATATCGGAATGCTGCAAGAATGGCTCTA
GTTGCAGAAGTGGATGCTTTAGAAATTCATGCCCATGGTGGTTATATCATTGATCAGTTTATGACACCAATTTGGAATAA
ACGAACCGATGAATACGGAGGAAGTTTTGAAAACCGCATGCGTCTAGTTACCGAAATATATCAGGCGATGAGAGATGAAG
TTGGCCCTAACTTCCCTATCTTAATCAGAATGGCAGCCGACCACGATTTTGAAGGTGGAAGAACTGTAGAAGAAAGTATC
GAAATTGTAAAGTATCTAGAAAAATTAGGAATGGATGCCTTTGATATAGATTTAGGCTGTTATGAACAAAAACAATGGAT
AGTTCCTTCTATTTATACAGGACTAGGATGTATGGCGGATGCCGCTGCCGCAATAAAAAAAGAAGTAAACGTTCCTGTAC
TCAATTCAGGTACGCACACACCTGAAACAGCAGTTGAAACTATAGAAAATGAAAAAGCTGATTTTATCATGCTAGGACGA
CCACTTATTGCAGACCCTGATTGGGTAAATAAACTCAGACTTGAAGGTCCAGAAGAAATCAAACCTTGTCTTTTCTGTAA
TGAAGTTTGTGTTGGAAGATTATATCAAAATAGAACCATATCATGTGCAATTAATCCCCACGCCGCATTTGAGAAAAAAT
ATCCTATGACAAAAACGGAGCTCCCTAAAAAAGTTGTTGTAGTCGGGGGTGGTCCTGGCGGAATGGAAGCAGCAAGAATA
GCAGCAAAAAAAGGTCACAGGGTCACGCTATACGAAAAGTCTGATAAACTAGGTGGCCAAATTAATGCCGCTGCAACACC
ACCTTTCAAAAAAAGATTACAAGATCTGATTGATTGGCAAGAAAGACAACTGAACAAATTTGGAGTAGATATCCGCTTAA
ACCACAAAATCACTGAGGAATCTTCAGAATTAGAGAATGTAGACCAAATCATCGTTGCAGTGGGTGCAAAACCCTTACTA
CCTAAAATTAAGGGGATTGATAAAGAAAATGTAGTTGAAGTTATCAGTGCACATCAAAATCCTGAGTTTGTAAAAGGAGA
CAATATCGTAGTTGCTGGAGGTGGTTTATCTGGCTGTGATTTTGCACTTGAAATGGCAATGGAAGGGAAGAAAGTTAGTA
TTGTTGAAATGTTAGATGAACTTGCGGCTACGGAACTAATGGATAATCGTAACCCATTACTATTTAAATTAAATGAATAT
AATGTAGAACAATTAACAGGACACAAAGTGATAGAGTTTACAGATACTGGTTTAACAGCCGAAAAAGCAGACGGAACGAA
AGTTGAACTCAAAGCAGATACTGTTGTTGCAGCCTTTGGAACGCAATCTGAAAGTGAACTGGCCGAAAGTATTTGGAAAA
AATACCCTACGTCACGATTGATCGGAGACTGTGTTGAAGTCGCACAAATTGGTAGCGCAGTTAGGAATGGATTTTTCGCT
GGATGGTCCATTGACTAA

Upstream 100 bases:

>100_bases
TTCGTTAACTACAACAATGTACAGAAAATTCAGTTATGATAAGCTTGACTTGTTAGTTAATTAACAAGAGTTCAATTCTA
TTGTACAGGGAGGAATCATA

Downstream 100 bases:

>100_bases
CATCTTGATTTTAGATTAACCATCTATTGATTAACAGTTATCCTTTGACTCAGGGCTAGCCATTACAGAATTCAATTAAT
TCATCCTCGCCTGTGTAAAC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MDKYSRLFEGVKIGDMKLRNRLVMSPMGTFNENYDGSISQEQIDYYEARAKGGVGMIIAEAQYVTNKTDPWIESMTAVGT
DIQMKGWARMAEAVHAHGAKICLQLSCGLGRNAFPFDSRQMVSASAVPSFFFPDQLCRPLEKDEIKDIVESYRNAARMAL
VAEVDALEIHAHGGYIIDQFMTPIWNKRTDEYGGSFENRMRLVTEIYQAMRDEVGPNFPILIRMAADHDFEGGRTVEESI
EIVKYLEKLGMDAFDIDLGCYEQKQWIVPSIYTGLGCMADAAAAIKKEVNVPVLNSGTHTPETAVETIENEKADFIMLGR
PLIADPDWVNKLRLEGPEEIKPCLFCNEVCVGRLYQNRTISCAINPHAAFEKKYPMTKTELPKKVVVVGGGPGGMEAARI
AAKKGHRVTLYEKSDKLGGQINAAATPPFKKRLQDLIDWQERQLNKFGVDIRLNHKITEESSELENVDQIIVAVGAKPLL
PKIKGIDKENVVEVISAHQNPEFVKGDNIVVAGGGLSGCDFALEMAMEGKKVSIVEMLDELAATELMDNRNPLLFKLNEY
NVEQLTGHKVIEFTDTGLTAEKADGTKVELKADTVVAAFGTQSESELAESIWKKYPTSRLIGDCVEVAQIGSAVRNGFFA
GWSID

Sequences:

>Translated_645_residues
MDKYSRLFEGVKIGDMKLRNRLVMSPMGTFNENYDGSISQEQIDYYEARAKGGVGMIIAEAQYVTNKTDPWIESMTAVGT
DIQMKGWARMAEAVHAHGAKICLQLSCGLGRNAFPFDSRQMVSASAVPSFFFPDQLCRPLEKDEIKDIVESYRNAARMAL
VAEVDALEIHAHGGYIIDQFMTPIWNKRTDEYGGSFENRMRLVTEIYQAMRDEVGPNFPILIRMAADHDFEGGRTVEESI
EIVKYLEKLGMDAFDIDLGCYEQKQWIVPSIYTGLGCMADAAAAIKKEVNVPVLNSGTHTPETAVETIENEKADFIMLGR
PLIADPDWVNKLRLEGPEEIKPCLFCNEVCVGRLYQNRTISCAINPHAAFEKKYPMTKTELPKKVVVVGGGPGGMEAARI
AAKKGHRVTLYEKSDKLGGQINAAATPPFKKRLQDLIDWQERQLNKFGVDIRLNHKITEESSELENVDQIIVAVGAKPLL
PKIKGIDKENVVEVISAHQNPEFVKGDNIVVAGGGLSGCDFALEMAMEGKKVSIVEMLDELAATELMDNRNPLLFKLNEY
NVEQLTGHKVIEFTDTGLTAEKADGTKVELKADTVVAAFGTQSESELAESIWKKYPTSRLIGDCVEVAQIGSAVRNGFFA
GWSID
>Mature_645_residues
MDKYSRLFEGVKIGDMKLRNRLVMSPMGTFNENYDGSISQEQIDYYEARAKGGVGMIIAEAQYVTNKTDPWIESMTAVGT
DIQMKGWARMAEAVHAHGAKICLQLSCGLGRNAFPFDSRQMVSASAVPSFFFPDQLCRPLEKDEIKDIVESYRNAARMAL
VAEVDALEIHAHGGYIIDQFMTPIWNKRTDEYGGSFENRMRLVTEIYQAMRDEVGPNFPILIRMAADHDFEGGRTVEESI
EIVKYLEKLGMDAFDIDLGCYEQKQWIVPSIYTGLGCMADAAAAIKKEVNVPVLNSGTHTPETAVETIENEKADFIMLGR
PLIADPDWVNKLRLEGPEEIKPCLFCNEVCVGRLYQNRTISCAINPHAAFEKKYPMTKTELPKKVVVVGGGPGGMEAARI
AAKKGHRVTLYEKSDKLGGQINAAATPPFKKRLQDLIDWQERQLNKFGVDIRLNHKITEESSELENVDQIIVAVGAKPLL
PKIKGIDKENVVEVISAHQNPEFVKGDNIVVAGGGLSGCDFALEMAMEGKKVSIVEMLDELAATELMDNRNPLLFKLNEY
NVEQLTGHKVIEFTDTGLTAEKADGTKVELKADTVVAAFGTQSESELAESIWKKYPTSRLIGDCVEVAQIGSAVRNGFFA
GWSID

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=538, Percent_Identity=29.368029739777, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1787939, Length=349, Percent_Identity=26.3610315186246, Blast_Score=112, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17559804, Length=351, Percent_Identity=29.3447293447293, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17565138, Length=343, Percent_Identity=29.1545189504373, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17564188, Length=317, Percent_Identity=28.7066246056782, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=228, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=264, Percent_Identity=31.0606060606061, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540738, Length=337, Percent_Identity=26.7062314540059, Blast_Score=91, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=262, Percent_Identity=28.2442748091603, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=350, Percent_Identity=27.4285714285714, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6321973, Length=351, Percent_Identity=26.2108262108262, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28574881, Length=218, Percent_Identity=27.0642201834862, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665539, Length=218, Percent_Identity=27.0642201834862, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665547, Length=218, Percent_Identity=27.0642201834862, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665543, Length=218, Percent_Identity=27.0642201834862, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 71486; Mature: 71486

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKYSRLFEGVKIGDMKLRNRLVMSPMGTFNENYDGSISQEQIDYYEARAKGGVGMIIAE
CCHHHHHHCCCCCCCHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEE
AQYVTNKTDPWIESMTAVGTDIQMKGWARMAEAVHAHGAKICLQLSCGLGRNAFPFDSRQ
CHHHCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHH
MVSASAVPSFFFPDQLCRPLEKDEIKDIVESYRNAARMALVAEVDALEIHAHGGYIIDQF
HHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCEEEEEECCCEEHHHH
MTPIWNKRTDEYGGSFENRMRLVTEIYQAMRDEVGPNFPILIRMAADHDFEGGRTVEESI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHH
EIVKYLEKLGMDAFDIDLGCYEQKQWIVPSIYTGLGCMADAAAAIKKEVNVPVLNSGTHT
HHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCC
PETAVETIENEKADFIMLGRPLIADPDWVNKLRLEGPEEIKPCLFCNEVCVGRLYQNRTI
HHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCEE
SCAINPHAAFEKKYPMTKTELPKKVVVVGGGPGGMEAARIAAKKGHRVTLYEKSDKLGGQ
EEEECCCHHHHCCCCCCHHCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCCE
INAAATPPFKKRLQDLIDWQERQLNKFGVDIRLNHKITEESSELENVDQIIVAVGAKPLL
EECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
PKIKGIDKENVVEVISAHQNPEFVKGDNIVVAGGGLSGCDFALEMAMEGKKVSIVEMLDE
HHHCCCCHHHHHHHHHCCCCCCEECCCCEEEECCCCCCHHHHHHHHHCCCEEHHHHHHHH
LAATELMDNRNPLLFKLNEYNVEQLTGHKVIEFTDTGLTAEKADGTKVELKADTVVAAFG
HHHHHHHCCCCCEEEEECCCCHHHHCCCEEEEEECCCCEEECCCCCEEEEEECEEEEEEC
TQSESELAESIWKKYPTSRLIGDCVEVAQIGSAVRNGFFAGWSID
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDKYSRLFEGVKIGDMKLRNRLVMSPMGTFNENYDGSISQEQIDYYEARAKGGVGMIIAE
CCHHHHHHCCCCCCCHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEE
AQYVTNKTDPWIESMTAVGTDIQMKGWARMAEAVHAHGAKICLQLSCGLGRNAFPFDSRQ
CHHHCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHH
MVSASAVPSFFFPDQLCRPLEKDEIKDIVESYRNAARMALVAEVDALEIHAHGGYIIDQF
HHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCEEEEEECCCEEHHHH
MTPIWNKRTDEYGGSFENRMRLVTEIYQAMRDEVGPNFPILIRMAADHDFEGGRTVEESI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHH
EIVKYLEKLGMDAFDIDLGCYEQKQWIVPSIYTGLGCMADAAAAIKKEVNVPVLNSGTHT
HHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCC
PETAVETIENEKADFIMLGRPLIADPDWVNKLRLEGPEEIKPCLFCNEVCVGRLYQNRTI
HHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCEE
SCAINPHAAFEKKYPMTKTELPKKVVVVGGGPGGMEAARIAAKKGHRVTLYEKSDKLGGQ
EEEECCCHHHHCCCCCCHHCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCCE
INAAATPPFKKRLQDLIDWQERQLNKFGVDIRLNHKITEESSELENVDQIIVAVGAKPLL
EECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
PKIKGIDKENVVEVISAHQNPEFVKGDNIVVAGGGLSGCDFALEMAMEGKKVSIVEMLDE
HHHCCCCHHHHHHHHHCCCCCCEECCCCEEEECCCCCCHHHHHHHHHCCCEEHHHHHHHH
LAATELMDNRNPLLFKLNEYNVEQLTGHKVIEFTDTGLTAEKADGTKVELKADTVVAAFG
HHHHHHHCCCCCEEEEECCCCHHHHCCCEEEEEECCCCEEECCCCCEEEEEECEEEEEEC
TQSESELAESIWKKYPTSRLIGDCVEVAQIGSAVRNGFFAGWSID
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]