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The map label for this gene is fadH [C]

Identifier: 188585595

GI number: 188585595

Start: 1028619

End: 1030553

Strand: Reverse

Name: fadH [C]

Synonym: Nther_0968

Alternate gene names: 188585595

Gene position: 1030553-1028619 (Counterclockwise)

Preceding gene: 188585613

Following gene: 188585594

Centisome position: 32.56

GC content: 40.67

Gene sequence:

>1935_bases
TTGAATTTTCCAAAATTATTTCAGCCAATAAAATTAGGGACAATGGAAGTTCCCAATCGATTTGTAGTCCCTGCCATGGG
TACTAATTTAGCTAATCCAGATGGAACCATTTCTGAAGATTTGATCGCCTACTGGGAAACCAGAGCTAAGGGTGGCTGGG
GACTTTTAACTGTGGAAGTTACAGCAGTTGATTCCCTAGGTAAAGCTATCCCAAATCAACCAGGACTTTGGGATGACCAA
TTCATAGAAGGATTTAGCAATTTAGCAAAAGCAGTGAAAAAGCACGATGCAAAAATAGCCGTGCAATTGCATCACGCAGG
AAGGCAAACCACATCGACTACAATTGGCGATCAGCCTGTAGCTCCATCACCTTTAAAATGTCCTATTTGCAATGAAATCC
CAAGAGAACTAAGCCGAGAAGAAATTTATGCTTTAATCGACTCTTATGCCACAGCCGCTTATAGGGCAAAAATGGCGGGA
ATTGACGCCGTAGAAATCCACGGTGCTCACGGATATCTGATTGCCCAGTTCATGTCTCCCCATTCCAATAAACGAGTCGA
CGAATTTGGCGGTACTTTTGAAAATCGCATGAGATTTCCCAAAGAAATTGCTAAAAATATTCGCCGTAAAGTTGGATCCG
GTTTCCCTGTCATTTTTAGACTTAGTGCCGATGAAAGAGTAGCTGACGGCTTGACAGTACAAGAGTCCAGGGCAGCAGCA
AAAATAATGGAACAAGCGGGTGTAGATGCCATTCATGTTTCTAAAGGGGTCTACGAAACTATGCAGTATATAGTTGCTCC
TTCTGATGTAGGTCCAGGTTACATTCTACCTGATGCCGGTGAAATAAAAAAATCAACAAATCTTCCTGTAATTGGAGTAG
GTAGAATTAATACACCCTATATGGCAGACGATGCGATCACAAGAAATCAGGCTGATCTACTCAGCTGGGGCAGGCAATCC
CTGACAGATCCTGAGCTACCTAATAAATTAGCTTCAGGGAAAACCGACGAAGTAGCACCTTGTATAGCTTGCAATCAAGG
TTGTATTGGCTACATTTTTGACCCAGATAAATTACAAATCAGTTGTCTAGTCAATCCTTTCTGTGGAAGCGAAAGAGAAA
TGGAAATAAATAATGCTCCAGAACAAAAGCATGTGGTAATCATAGGAGCAGGTCCAGGCGGTCTGAAAGCTGCGTGGATA
TTAGCGGAAAGAGGCCATCAAGTAACTGTCTTTGAAAGCCGTTCAAAACCAGGAGGTCAAATGGAGTTAGGAGCTTTTCC
ACCAGGAAGACAGGAAATAACGAAATTTATCAATTATTACTGGCATATGGCATTGAAAAATGGAGTTACCTTCAGATTTA
ACACAGAAGCCACTGTTCAAGATATAGTTGATGAAAATCCCGATAGCATAATTCTGGCTACAGGCGGTAAACCTCTAATT
CCAGAAAATATATCTGGAATTAATCAAGAACATGTAGTAACTGCTTTAGATATACTCATGGGAAAAATCACTCCTGAAAG
CCGAGTTTTGATAATAGGTGGTGGCTTGATTGGATCTGAAACTGCAGATTTTCTAGCAATGAAAGGACATAAAGTAACTC
TTTGTGAACAGGAAGCAGAAATTGCAACGGATATTCATGAAGCCGTTAAATACTTTTTGATTCAGAGACTCCACGAAAAT
CAGGTAGATATTAAAACTGGCACAAAAATAATCGAAATCCAAAAAGATAAAGTTATTGCAAGTATCAATGGTGAACGCCA
TGAGTTATCTGGACATGAAACGATAGTTCTAGCCGCGGGAACTGTTTCTAATAATCAGCTAGGTCCTGAGCTCTCAAACA
AAGTCAACAACATTCATGTCATAGGGGATGCTAGTGATCCTAGAAAAGCCATTGATGCAATAGAAGAAGGGGCCAGAGTA
GCTTTGAATATTTAA

Upstream 100 bases:

>100_bases
GTTGTTGATGCTGAAATTGAAATTTTACAGTTATCAACATTCAATTAATACAGCCTAAAAAAATTACAAGTTTGCCATAA
CAAATTAAGGAGTTGATAAA

Downstream 100 bases:

>100_bases
TTAAGAAAATAGACTTCTAGTTAATAAACAGGGTCAAACTTAAATTGGCCCTGTTACCCTTAGATTTCAGGAGGCATAAC
TATGTTTTGGAAAAAATATC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MNFPKLFQPIKLGTMEVPNRFVVPAMGTNLANPDGTISEDLIAYWETRAKGGWGLLTVEVTAVDSLGKAIPNQPGLWDDQ
FIEGFSNLAKAVKKHDAKIAVQLHHAGRQTTSTTIGDQPVAPSPLKCPICNEIPRELSREEIYALIDSYATAAYRAKMAG
IDAVEIHGAHGYLIAQFMSPHSNKRVDEFGGTFENRMRFPKEIAKNIRRKVGSGFPVIFRLSADERVADGLTVQESRAAA
KIMEQAGVDAIHVSKGVYETMQYIVAPSDVGPGYILPDAGEIKKSTNLPVIGVGRINTPYMADDAITRNQADLLSWGRQS
LTDPELPNKLASGKTDEVAPCIACNQGCIGYIFDPDKLQISCLVNPFCGSEREMEINNAPEQKHVVIIGAGPGGLKAAWI
LAERGHQVTVFESRSKPGGQMELGAFPPGRQEITKFINYYWHMALKNGVTFRFNTEATVQDIVDENPDSIILATGGKPLI
PENISGINQEHVVTALDILMGKITPESRVLIIGGGLIGSETADFLAMKGHKVTLCEQEAEIATDIHEAVKYFLIQRLHEN
QVDIKTGTKIIEIQKDKVIASINGERHELSGHETIVLAAGTVSNNQLGPELSNKVNNIHVIGDASDPRKAIDAIEEGARV
ALNI

Sequences:

>Translated_644_residues
MNFPKLFQPIKLGTMEVPNRFVVPAMGTNLANPDGTISEDLIAYWETRAKGGWGLLTVEVTAVDSLGKAIPNQPGLWDDQ
FIEGFSNLAKAVKKHDAKIAVQLHHAGRQTTSTTIGDQPVAPSPLKCPICNEIPRELSREEIYALIDSYATAAYRAKMAG
IDAVEIHGAHGYLIAQFMSPHSNKRVDEFGGTFENRMRFPKEIAKNIRRKVGSGFPVIFRLSADERVADGLTVQESRAAA
KIMEQAGVDAIHVSKGVYETMQYIVAPSDVGPGYILPDAGEIKKSTNLPVIGVGRINTPYMADDAITRNQADLLSWGRQS
LTDPELPNKLASGKTDEVAPCIACNQGCIGYIFDPDKLQISCLVNPFCGSEREMEINNAPEQKHVVIIGAGPGGLKAAWI
LAERGHQVTVFESRSKPGGQMELGAFPPGRQEITKFINYYWHMALKNGVTFRFNTEATVQDIVDENPDSIILATGGKPLI
PENISGINQEHVVTALDILMGKITPESRVLIIGGGLIGSETADFLAMKGHKVTLCEQEAEIATDIHEAVKYFLIQRLHEN
QVDIKTGTKIIEIQKDKVIASINGERHELSGHETIVLAAGTVSNNQLGPELSNKVNNIHVIGDASDPRKAIDAIEEGARV
ALNI
>Mature_644_residues
MNFPKLFQPIKLGTMEVPNRFVVPAMGTNLANPDGTISEDLIAYWETRAKGGWGLLTVEVTAVDSLGKAIPNQPGLWDDQ
FIEGFSNLAKAVKKHDAKIAVQLHHAGRQTTSTTIGDQPVAPSPLKCPICNEIPRELSREEIYALIDSYATAAYRAKMAG
IDAVEIHGAHGYLIAQFMSPHSNKRVDEFGGTFENRMRFPKEIAKNIRRKVGSGFPVIFRLSADERVADGLTVQESRAAA
KIMEQAGVDAIHVSKGVYETMQYIVAPSDVGPGYILPDAGEIKKSTNLPVIGVGRINTPYMADDAITRNQADLLSWGRQS
LTDPELPNKLASGKTDEVAPCIACNQGCIGYIFDPDKLQISCLVNPFCGSEREMEINNAPEQKHVVIIGAGPGGLKAAWI
LAERGHQVTVFESRSKPGGQMELGAFPPGRQEITKFINYYWHMALKNGVTFRFNTEATVQDIVDENPDSIILATGGKPLI
PENISGINQEHVVTALDILMGKITPESRVLIIGGGLIGSETADFLAMKGHKVTLCEQEAEIATDIHEAVKYFLIQRLHEN
QVDIKTGTKIIEIQKDKVIASINGERHELSGHETIVLAAGTVSNNQLGPELSNKVNNIHVIGDASDPRKAIDAIEEGARV
ALNI

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=687, Percent_Identity=34.3522561863173, Blast_Score=338, Evalue=7e-94,
Organism=Escherichia coli, GI1787939, Length=363, Percent_Identity=29.7520661157025, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17564188, Length=342, Percent_Identity=27.7777777777778, Blast_Score=127, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17559804, Length=342, Percent_Identity=28.3625730994152, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17559802, Length=338, Percent_Identity=30.4733727810651, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI72001454, Length=351, Percent_Identity=28.2051282051282, Blast_Score=123, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17540738, Length=340, Percent_Identity=28.8235294117647, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17565138, Length=335, Percent_Identity=28.6567164179104, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17566914, Length=323, Percent_Identity=26.625386996904, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17566916, Length=262, Percent_Identity=28.6259541984733, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6325086, Length=361, Percent_Identity=27.4238227146814, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6321973, Length=373, Percent_Identity=26.2734584450402, Blast_Score=107, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 70040; Mature: 70040

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFPKLFQPIKLGTMEVPNRFVVPAMGTNLANPDGTISEDLIAYWETRAKGGWGLLTVEV
CCCHHHHCCCCCCCEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEE
TAVDSLGKAIPNQPGLWDDQFIEGFSNLAKAVKKHDAKIAVQLHHAGRQTTSTTIGDQPV
EEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCC
APSPLKCPICNEIPRELSREEIYALIDSYATAAYRAKMAGIDAVEIHGAHGYLIAQFMSP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEHHCC
HSNKRVDEFGGTFENRMRFPKEIAKNIRRKVGSGFPVIFRLSADERVADGLTVQESRAAA
CCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCEEHHHHHHH
KIMEQAGVDAIHVSKGVYETMQYIVAPSDVGPGYILPDAGEIKKSTNLPVIGVGRINTPY
HHHHHCCCCEEEECHHHHHHHHHEECCCCCCCCEECCCCHHHHHCCCCCEEEECCCCCCC
MADDAITRNQADLLSWGRQSLTDPELPNKLASGKTDEVAPCIACNQGCIGYIFDPDKLQI
CCCCHHCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCEECCCCCEEEEECCCCEEE
SCLVNPFCGSEREMEINNAPEQKHVVIIGAGPGGLKAAWILAERGHQVTVFESRSKPGGQ
EEEECCCCCCCCEEECCCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCC
MELGAFPPGRQEITKFINYYWHMALKNGVTFRFNTEATVQDIVDENPDSIILATGGKPLI
EEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCEEEEECCCCCCC
PENISGINQEHVVTALDILMGKITPESRVLIIGGGLIGSETADFLAMKGHKVTLCEQEAE
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHEECCCEEEEECCHHH
IATDIHEAVKYFLIQRLHENQVDIKTGTKIIEIQKDKVIASINGERHELSGHETIVLAAG
HHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCEEEEEECCCCEECCCCCEEEEEEC
TVSNNQLGPELSNKVNNIHVIGDASDPRKAIDAIEEGARVALNI
CCCCCCCCCHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MNFPKLFQPIKLGTMEVPNRFVVPAMGTNLANPDGTISEDLIAYWETRAKGGWGLLTVEV
CCCHHHHCCCCCCCEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEE
TAVDSLGKAIPNQPGLWDDQFIEGFSNLAKAVKKHDAKIAVQLHHAGRQTTSTTIGDQPV
EEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCC
APSPLKCPICNEIPRELSREEIYALIDSYATAAYRAKMAGIDAVEIHGAHGYLIAQFMSP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEHHCC
HSNKRVDEFGGTFENRMRFPKEIAKNIRRKVGSGFPVIFRLSADERVADGLTVQESRAAA
CCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCEEHHHHHHH
KIMEQAGVDAIHVSKGVYETMQYIVAPSDVGPGYILPDAGEIKKSTNLPVIGVGRINTPY
HHHHHCCCCEEEECHHHHHHHHHEECCCCCCCCEECCCCHHHHHCCCCCEEEECCCCCCC
MADDAITRNQADLLSWGRQSLTDPELPNKLASGKTDEVAPCIACNQGCIGYIFDPDKLQI
CCCCHHCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCEECCCCCEEEEECCCCEEE
SCLVNPFCGSEREMEINNAPEQKHVVIIGAGPGGLKAAWILAERGHQVTVFESRSKPGGQ
EEEECCCCCCCCEEECCCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCC
MELGAFPPGRQEITKFINYYWHMALKNGVTFRFNTEATVQDIVDENPDSIILATGGKPLI
EEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCEEEEECCCCCCC
PENISGINQEHVVTALDILMGKITPESRVLIIGGGLIGSETADFLAMKGHKVTLCEQEAE
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHEECCCEEEEECCHHH
IATDIHEAVKYFLIQRLHENQVDIKTGTKIIEIQKDKVIASINGERHELSGHETIVLAAG
HHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCEEEEEECCCCEECCCCCEEEEEEC
TVSNNQLGPELSNKVNNIHVIGDASDPRKAIDAIEEGARVALNI
CCCCCCCCCHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]