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The map label for this gene is asnO [H]

Identifier: 188585587

GI number: 188585587

Start: 1017196

End: 1019049

Strand: Reverse

Name: asnO [H]

Synonym: Nther_0960

Alternate gene names: 188585587

Gene position: 1019049-1017196 (Counterclockwise)

Preceding gene: 188585594

Following gene: 188585586

Centisome position: 32.19

GC content: 34.68

Gene sequence:

>1854_bases
ATGTGTGGAATAACAGGTTGGGTTAATTTTAAAAAAAATTTGGAGGAAGAGGAAAAACAAATTAAACAAATGACGAACAC
TTTGGAACACCGTGGTCCTGATGATAAACAAATTTGGCTTGATAGACACGTAGCATTTGGTCATACTCGATTGGCTGTAA
TCGATCCTGCTTTTGGTAAACAGCCTATGAGTAAAGAATGTAGAAGGTCTCAATTCACTATTGTTTATAATGGAGAATTA
TATAATACAGAACAACTGCGATTAGAACTTAAGTCTCTAGGACATCACTTCACCACTAATTCTGATACTGAAGTTCTACT
TACTTCATTTATTGAATGGAAAGAGAAATGCTTGGAAAAGTTAAACGGAATTTTTGCCTTTGCTATTTGGGACAAAAATA
AAGAAAGATTGTTTATGGCAAGAGACAGATTAGGTGTTAAACCTTTATTTTACGCTATCAAACAGGAAGGTTTAATATTC
GGTTCTGAACTTAAAGCATTACTATCTAATAGATATATTGACAGTCATATTGATTTGGAAGGTCTGAAAGAAATTTTTGC
CCTTTCTCCATCTAGGACTCCAGGTCATGGTGTTTTTTCCGACATTTCAGAGCTTCGGCCAGCTCATGCAATGGTTTTTG
ATAAAGACAACTTCAAGATTTGGAGATACTGGCAAGTTAAAAGTTCAGTGCACAAAGATAGTAAAAAAGTAACTGCTTTA
AAAGTACACGATCTATTGAAGGATGCGGTTCAAAGACAGTTGGTATCGGATGTTCCAATTTGTACTTTTTTATCAGGAGG
AATAGATTCTTCCGCTTTAACAGCTTTTGCTTCTAAAAAATTGACACGAAAAGAGGGTTATAATCCACTAAACACCTATT
CAATCTCATATGAAGAAAATGAGCGTTATTTCTCATCGAATAATTTTCAGCCCACTGGTGATAAACAGTGGATTGATGTA
GTTTCCAACCAATTTGGCACAACTCATCATCAAAATGTCATTTCAATAGAGACTCTGGTAAACTATTTAAAAGAAGCGGT
TTATGTTAGAGATCTACCCGGTATGGCAGATATTGATTCTTCACTATTATGGTTTTGTAAACAAATAAAAAAGAATGCAA
CAGTAGCTCAATCAGGTGAATGTGCTGACGAAATTTTTGGTGGATACCCCTGGTTTTATCGTGAAGAACTAATGAATCGT
CCTTATTTCCCATGGATGGATTCAATAAATTTGCGGGAAAGATTATTAAGAGAACCTTGGAAAAGCAAATTACAATTGGA
AGATTATGTTCGTTTTAGATATCAAGAATCTATACAAGAGACTCCTTATTTAGAAGGAGAAAAGAGTGTAAACAAACAAA
GACGTGAAATTTCCTATTTGAATATGATCTGGTTTATGACTACTCTTTTGGATAGAAAAGACCGGATGAGTATGGGGGCT
AGTTTAGAGGTTCGTGTACCTTTTGCTGATCATAATTTAGTCGAATATGTTTGGAATATCCCAGCCTACATGAAATGGCA
TAATCAACAAGAAAAGTGGATTTTAAGAAAAGCTCTTGAAGATGTGTTGCCAGACGCTATATTAAATCGAAAAAAGAATC
CTTACCCCAAAACCTATCACCCAAAATACAGTCAAGCAGTTGCTACATGGGTGCAAACTGAAATTTTAGATGATCCGAAT
GCCCCATTGCTTGAATTTATCCAAAAAGATACCCTTGAAGATCTTGTTAAAAGCAAAGGAACCTCTATTAAAACTCCTTT
TTTTGGGCAATTAATGTCTGGTCCACAATTACTAGCACACTTAGGGCAAGTTAATACTTGGTTAAAAAAATACGATGTCA
AGATTAAATATTAA

Upstream 100 bases:

>100_bases
TTCTTTGTTAACTCATGATAAAATGCACGAAACTTAATCTCAGCTAATAGTATAATAGAAACCATGATAATGAAAATCAT
TTTCAAAAGGGTGGCTAATA

Downstream 100 bases:

>100_bases
CTTATATAATTTAAGTTTAATTATTTGACTTTAAGTTATTGCAGCTCCGAGAAATTTTACCCGGAGCTGCTTTTATTAGA
GCAATATTAGAATAATAATT

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MCGITGWVNFKKNLEEEEKQIKQMTNTLEHRGPDDKQIWLDRHVAFGHTRLAVIDPAFGKQPMSKECRRSQFTIVYNGEL
YNTEQLRLELKSLGHHFTTNSDTEVLLTSFIEWKEKCLEKLNGIFAFAIWDKNKERLFMARDRLGVKPLFYAIKQEGLIF
GSELKALLSNRYIDSHIDLEGLKEIFALSPSRTPGHGVFSDISELRPAHAMVFDKDNFKIWRYWQVKSSVHKDSKKVTAL
KVHDLLKDAVQRQLVSDVPICTFLSGGIDSSALTAFASKKLTRKEGYNPLNTYSISYEENERYFSSNNFQPTGDKQWIDV
VSNQFGTTHHQNVISIETLVNYLKEAVYVRDLPGMADIDSSLLWFCKQIKKNATVAQSGECADEIFGGYPWFYREELMNR
PYFPWMDSINLRERLLREPWKSKLQLEDYVRFRYQESIQETPYLEGEKSVNKQRREISYLNMIWFMTTLLDRKDRMSMGA
SLEVRVPFADHNLVEYVWNIPAYMKWHNQQEKWILRKALEDVLPDAILNRKKNPYPKTYHPKYSQAVATWVQTEILDDPN
APLLEFIQKDTLEDLVKSKGTSIKTPFFGQLMSGPQLLAHLGQVNTWLKKYDVKIKY

Sequences:

>Translated_617_residues
MCGITGWVNFKKNLEEEEKQIKQMTNTLEHRGPDDKQIWLDRHVAFGHTRLAVIDPAFGKQPMSKECRRSQFTIVYNGEL
YNTEQLRLELKSLGHHFTTNSDTEVLLTSFIEWKEKCLEKLNGIFAFAIWDKNKERLFMARDRLGVKPLFYAIKQEGLIF
GSELKALLSNRYIDSHIDLEGLKEIFALSPSRTPGHGVFSDISELRPAHAMVFDKDNFKIWRYWQVKSSVHKDSKKVTAL
KVHDLLKDAVQRQLVSDVPICTFLSGGIDSSALTAFASKKLTRKEGYNPLNTYSISYEENERYFSSNNFQPTGDKQWIDV
VSNQFGTTHHQNVISIETLVNYLKEAVYVRDLPGMADIDSSLLWFCKQIKKNATVAQSGECADEIFGGYPWFYREELMNR
PYFPWMDSINLRERLLREPWKSKLQLEDYVRFRYQESIQETPYLEGEKSVNKQRREISYLNMIWFMTTLLDRKDRMSMGA
SLEVRVPFADHNLVEYVWNIPAYMKWHNQQEKWILRKALEDVLPDAILNRKKNPYPKTYHPKYSQAVATWVQTEILDDPN
APLLEFIQKDTLEDLVKSKGTSIKTPFFGQLMSGPQLLAHLGQVNTWLKKYDVKIKY
>Mature_617_residues
MCGITGWVNFKKNLEEEEKQIKQMTNTLEHRGPDDKQIWLDRHVAFGHTRLAVIDPAFGKQPMSKECRRSQFTIVYNGEL
YNTEQLRLELKSLGHHFTTNSDTEVLLTSFIEWKEKCLEKLNGIFAFAIWDKNKERLFMARDRLGVKPLFYAIKQEGLIF
GSELKALLSNRYIDSHIDLEGLKEIFALSPSRTPGHGVFSDISELRPAHAMVFDKDNFKIWRYWQVKSSVHKDSKKVTAL
KVHDLLKDAVQRQLVSDVPICTFLSGGIDSSALTAFASKKLTRKEGYNPLNTYSISYEENERYFSSNNFQPTGDKQWIDV
VSNQFGTTHHQNVISIETLVNYLKEAVYVRDLPGMADIDSSLLWFCKQIKKNATVAQSGECADEIFGGYPWFYREELMNR
PYFPWMDSINLRERLLREPWKSKLQLEDYVRFRYQESIQETPYLEGEKSVNKQRREISYLNMIWFMTTLLDRKDRMSMGA
SLEVRVPFADHNLVEYVWNIPAYMKWHNQQEKWILRKALEDVLPDAILNRKKNPYPKTYHPKYSQAVATWVQTEILDDPN
APLLEFIQKDTLEDLVKSKGTSIKTPFFGQLMSGPQLLAHLGQVNTWLKKYDVKIKY

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=423, Percent_Identity=25.7683215130024, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI168229252, Length=423, Percent_Identity=25.7683215130024, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI168229250, Length=423, Percent_Identity=25.7683215130024, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI296010848, Length=396, Percent_Identity=25.7575757575758, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI296010852, Length=324, Percent_Identity=24.3827160493827, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI296010850, Length=324, Percent_Identity=24.3827160493827, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786889, Length=414, Percent_Identity=26.8115942028986, Blast_Score=152, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI71993933, Length=573, Percent_Identity=25.8289703315881, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25147557, Length=573, Percent_Identity=25.8289703315881, Blast_Score=132, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17560178, Length=551, Percent_Identity=25.0453720508167, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25147560, Length=344, Percent_Identity=26.1627906976744, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321563, Length=573, Percent_Identity=25.6544502617801, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6325403, Length=573, Percent_Identity=23.0366492146597, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI45553209, Length=569, Percent_Identity=24.2530755711775, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72073; Mature: 72073

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWVNFKKNLEEEEKQIKQMTNTLEHRGPDDKQIWLDRHVAFGHTRLAVIDPAFGK
CCCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHEECCCCEEEEECCCCCC
QPMSKECRRSQFTIVYNGELYNTEQLRLELKSLGHHFTTNSDTEVLLTSFIEWKEKCLEK
CCCHHHHCCCCEEEEECCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFAIWDKNKERLFMARDRLGVKPLFYAIKQEGLIFGSELKALLSNRYIDSHIDLE
HCCEEEEEEECCCCCCEEEEHHCCCCHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCHH
GLKEIFALSPSRTPGHGVFSDISELRPAHAMVFDKDNFKIWRYWQVKSSVHKDSKKVTAL
HHHHHHHCCCCCCCCCCHHHHHHHCCCHHEEEEECCCCEEEEEEEHHHHHHCCCHHHHHH
KVHDLLKDAVQRQLVSDVPICTFLSGGIDSSALTAFASKKLTRKEGYNPLNTYSISYEEN
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
ERYFSSNNFQPTGDKQWIDVVSNQFGTTHHQNVISIETLVNYLKEAVYVRDLPGMADIDS
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCHH
SLLWFCKQIKKNATVAQSGECADEIFGGYPWFYREELMNRPYFPWMDSINLRERLLREPW
HHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCHH
KSKLQLEDYVRFRYQESIQETPYLEGEKSVNKQRREISYLNMIWFMTTLLDRKDRMSMGA
HHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
SLEVRVPFADHNLVEYVWNIPAYMKWHNQQEKWILRKALEDVLPDAILNRKKNPYPKTYH
CEEEEECCCCCHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCC
PKYSQAVATWVQTEILDDPNAPLLEFIQKDTLEDLVKSKGTSIKTPFFGQLMSGPQLLAH
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHH
LGQVNTWLKKYDVKIKY
HHHHHHHHHHHCCEECC
>Mature Secondary Structure
MCGITGWVNFKKNLEEEEKQIKQMTNTLEHRGPDDKQIWLDRHVAFGHTRLAVIDPAFGK
CCCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHEECCCCEEEEECCCCCC
QPMSKECRRSQFTIVYNGELYNTEQLRLELKSLGHHFTTNSDTEVLLTSFIEWKEKCLEK
CCCHHHHCCCCEEEEECCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFAIWDKNKERLFMARDRLGVKPLFYAIKQEGLIFGSELKALLSNRYIDSHIDLE
HCCEEEEEEECCCCCCEEEEHHCCCCHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCHH
GLKEIFALSPSRTPGHGVFSDISELRPAHAMVFDKDNFKIWRYWQVKSSVHKDSKKVTAL
HHHHHHHCCCCCCCCCCHHHHHHHCCCHHEEEEECCCCEEEEEEEHHHHHHCCCHHHHHH
KVHDLLKDAVQRQLVSDVPICTFLSGGIDSSALTAFASKKLTRKEGYNPLNTYSISYEEN
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
ERYFSSNNFQPTGDKQWIDVVSNQFGTTHHQNVISIETLVNYLKEAVYVRDLPGMADIDS
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCHH
SLLWFCKQIKKNATVAQSGECADEIFGGYPWFYREELMNRPYFPWMDSINLRERLLREPW
HHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCHH
KSKLQLEDYVRFRYQESIQETPYLEGEKSVNKQRREISYLNMIWFMTTLLDRKDRMSMGA
HHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
SLEVRVPFADHNLVEYVWNIPAYMKWHNQQEKWILRKALEDVLPDAILNRKKNPYPKTYH
CEEEEECCCCCHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCC
PKYSQAVATWVQTEILDDPNAPLLEFIQKDTLEDLVKSKGTSIKTPFFGQLMSGPQLLAH
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHH
LGQVNTWLKKYDVKIKY
HHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]