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The map label for this gene is cbiF [H]

Identifier: 188585561

GI number: 188585561

Start: 991154

End: 991936

Strand: Direct

Name: cbiF [H]

Synonym: Nther_0933

Alternate gene names: 188585561

Gene position: 991154-991936 (Clockwise)

Preceding gene: 188585560

Following gene: 188585562

Centisome position: 31.31

GC content: 39.34

Gene sequence:

>783_bases
ATGGACAGATCACAGCAGCCAAAAGTCTATTTTTTAGGAGCAGGTCCTGGTGACCCGGAACTTTTAACGAGGAAAGGGGA
AAAATTGCTGACTGATTGTGATGCTGTAGTATATGCAGGTTCTTTAATTAATCCGGCTTTGTTGAAGCTAGTTTCTTCAA
ATTGTCAATTATATGATAGTGCTGAGCTCTCCAAAGAAGAGATTACGGATATTTTGATTAATCATTCCCGAAAAGGAGAT
AAAATAGTGCGGCTTCATTCGGGAGATCCTGGCCTTTACGGAGCCATTCAGGAACAGATAGATGAACTGAAAGGATGGGG
TATTGAAGTTGAAATTATTCCAGGTATTAGCGCTTATCAAGGGGCAATGGCAGCTTTAAAAAGGGAATTTACCTTGCCGG
GTGTTACTCAGACAGTTATTCTAACACGACATGGAAAAAGAACACCTGTCCCGGAGAAAGAACAGTTAAAAAACCTGGCT
AAAAATGAAAGTACCATGTGTATCTATCTTAGTACTCATTTAATAACTGAAGTAGTTCGAGAATTAACTGAAGGGGGATT
ATCCGGTACCACACCAGTCAGGGTAGTATATAAGGCCACCTGGCCTGATGAACAAGTGATTAGCGGAACCTTAAATAATA
TATCTCAAAAAGTTGCTGATGCTGAAATTTCCAAAACAGCTTTGATTGTTGTAGGACATGTACTTTCTTCATCGGACTAT
CAAGAAAGCAAATTGTATGATAAACACTTCAGCCATGGTTTTAGAAAAGGAAAGGAAGAGTAG

Upstream 100 bases:

>100_bases
GATGAGAAATTATATCAAAATATCTTGGAAGTGGAACCTAGAAAAGTCAGTTATCTATCAACACTGATAGTCTCAAAAAA
TTTAGGAAGCAGGTGATACT

Downstream 100 bases:

>100_bases
TGATGAGTTGGGCTTTTAAGAGAATTTCCATTATTGCCGTGAGCCGTGAAGGCGTTAATCTGGGATTGAAATTATTTCAA
CACTTTACTGAAAGTACTTT

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MDRSQQPKVYFLGAGPGDPELLTRKGEKLLTDCDAVVYAGSLINPALLKLVSSNCQLYDSAELSKEEITDILINHSRKGD
KIVRLHSGDPGLYGAIQEQIDELKGWGIEVEIIPGISAYQGAMAALKREFTLPGVTQTVILTRHGKRTPVPEKEQLKNLA
KNESTMCIYLSTHLITEVVRELTEGGLSGTTPVRVVYKATWPDEQVISGTLNNISQKVADAEISKTALIVVGHVLSSSDY
QESKLYDKHFSHGFRKGKEE

Sequences:

>Translated_260_residues
MDRSQQPKVYFLGAGPGDPELLTRKGEKLLTDCDAVVYAGSLINPALLKLVSSNCQLYDSAELSKEEITDILINHSRKGD
KIVRLHSGDPGLYGAIQEQIDELKGWGIEVEIIPGISAYQGAMAALKREFTLPGVTQTVILTRHGKRTPVPEKEQLKNLA
KNESTMCIYLSTHLITEVVRELTEGGLSGTTPVRVVYKATWPDEQVISGTLNNISQKVADAEISKTALIVVGHVLSSSDY
QESKLYDKHFSHGFRKGKEE
>Mature_260_residues
MDRSQQPKVYFLGAGPGDPELLTRKGEKLLTDCDAVVYAGSLINPALLKLVSSNCQLYDSAELSKEEITDILINHSRKGD
KIVRLHSGDPGLYGAIQEQIDELKGWGIEVEIIPGISAYQGAMAALKREFTLPGVTQTVILTRHGKRTPVPEKEQLKNLA
KNESTMCIYLSTHLITEVVRELTEGGLSGTTPVRVVYKATWPDEQVISGTLNNISQKVADAEISKTALIVVGHVLSSSDY
QESKLYDKHFSHGFRKGKEE

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=239, Percent_Identity=30.5439330543933, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322922, Length=266, Percent_Identity=22.5563909774436, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28591; Mature: 28591

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRSQQPKVYFLGAGPGDPELLTRKGEKLLTDCDAVVYAGSLINPALLKLVSSNCQLYDS
CCCCCCCCEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHCHHCCHHHHHHHHCCCCEECC
AELSKEEITDILINHSRKGDKIVRLHSGDPGLYGAIQEQIDELKGWGIEVEIIPGISAYQ
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHH
GAMAALKREFTLPGVTQTVILTRHGKRTPVPEKEQLKNLAKNESTMCIYLSTHLITEVVR
HHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHH
ELTEGGLSGTTPVRVVYKATWPDEQVISGTLNNISQKVADAEISKTALIVVGHVLSSSDY
HHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCCCC
QESKLYDKHFSHGFRKGKEE
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDRSQQPKVYFLGAGPGDPELLTRKGEKLLTDCDAVVYAGSLINPALLKLVSSNCQLYDS
CCCCCCCCEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHCHHCCHHHHHHHHCCCCEECC
AELSKEEITDILINHSRKGDKIVRLHSGDPGLYGAIQEQIDELKGWGIEVEIIPGISAYQ
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHH
GAMAALKREFTLPGVTQTVILTRHGKRTPVPEKEQLKNLAKNESTMCIYLSTHLITEVVR
HHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHH
ELTEGGLSGTTPVRVVYKATWPDEQVISGTLNNISQKVADAEISKTALIVVGHVLSSSDY
HHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCCCC
QESKLYDKHFSHGFRKGKEE
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]