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The map label for this gene is argH

Identifier: 188585550

GI number: 188585550

Start: 978089

End: 979552

Strand: Reverse

Name: argH

Synonym: Nther_0922

Alternate gene names: 188585550

Gene position: 979552-978089 (Counterclockwise)

Preceding gene: 188585551

Following gene: 188585549

Centisome position: 30.94

GC content: 37.02

Gene sequence:

>1464_bases
TTGTCAAAACTCTGGGGTGGAAGATTTTCAAAACAAACAGATCAACTAGTAGATGAATTTAATGCTTCCATCGAATTTGA
TAACCGGTTAATATTTTATGATCTTCTTGGCAGTCAAGCTCATGTAAAAATGCTGTTCCAACAGGGCATCATCGACACAA
CAGATTACAAACAGATCACAGAAGGCTTGGATATAATCTGGAAAGAAGCCGAGCAGGATAAACTCGAATTCAACTTAAGT
GACGAAGATATTCACATGAGTATTGAAAAACGTTTGATAGAACTCAAAGGTGAAGTAGGCAAAAAACTTCATACAGCCAG
AAGCAGAAATGATCAGGTAGCTGTAGATATGAATATGTTCTTATGTGATAAGGCCATAAAGTTAGTAAATGAATTATTAC
AAAATATGTCAGTTATTAAAGAACTTTCTGAGCAGCATACCAAGACTTATATGCCGGGTTACACTCATCTTCAACGGGCA
CAACCTACAACTTTGGGCCATCACATGCTAAATTACTTTTGGAAATTTCAAAGAGATGCTAGCCGGTTGATAGACTTTCG
CAACAGGGCTGATCTTTCTCCATTAGGGAGTGGGGCTTTTGCCGGAACGGGGTTTAATATCTCAAGGAGGTCCACTAGAA
AAGACTTAGGGTTCACACAACAGTTTGAAAATAGTATGGATGCCGTTAGCAGTAGAGATCTCTCCTTAGAATTTATCTTT
TGTTTAAGCTCTATCATGATCAATTTAAGTCGCCTAGCCGAGGAACTCATTCTATGGTCTACTAAGGAATTTGACTTTAT
TGAATTAGATGATGCCTTTGCTACTGGTTCGAGCATTATGCCCCAAAAGAAAAATCCGGATGTACCAGAATTAATCAGAG
GTAAAACAGGCAGAGTAGTTGGTCACTTAACTGCACTAGCCACTACATATAAGGGATTACCCATGGCATATAACAAAGAT
TTTCAGGAGGATAAAGAAGGACTATTTGATAGTTTAGATACCGTGGAAAGTTCCCTAAAGCTAACATCTAAAATACTATC
TACCATGACCATCAAAACAAAAAACATGGAAAAAGCACTATACCAAGATTTTTCAAATGCCACAGACATCGCTGATTACC
TGGCAACTCAGGGTATCCCATTTAGAGATGCACATGCAGTTGTAGGTCAACTTGTAAAACATTGTCAAGAACATAATAAA
CTGTTCTATCAATTAACTGAACAGGAGCTCGATATTTCATTTAAACAGATAGCCGATTCTTTAGAAGAAAACAGTCAACA
AATACTAACTCAAATGGACACAACTAAAATATTAAATATTATGGATCCAATCAAATGTGTCCATAACAGAAACAGTAGAG
GTGCACCAGCACCGGAAGCCCTTCAATTTCAATTGGGACAAGCTCAAAAATATTTTCATAGCCTAACAGAGCAAGTTAAA
ACATATCAAAGCTTTTTACCTTAA

Upstream 100 bases:

>100_bases
AAGTTTATAATCAAAAAGATGCTGAAGGATTCATCAACTTATTTGGTCTCACTTTAACAACCGAAGGTATTGTCAAAAGA
GGCAAAGGAGGAGAATAACA

Downstream 100 bases:

>100_bases
AAATGCTTTAAAAAGGAGGACATACACATGGAACATTTCGGAATTCTATCATTAGTACCGCCAGCCCTGGCTATAGGGCT
AGCGGTACTAACAAAAAATA

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSKLWGGRFSKQTDQLVDEFNASIEFDNRLIFYDLLGSQAHVKMLFQQGIIDTTDYKQITEGLDIIWKEAEQDKLEFNLS
DEDIHMSIEKRLIELKGEVGKKLHTARSRNDQVAVDMNMFLCDKAIKLVNELLQNMSVIKELSEQHTKTYMPGYTHLQRA
QPTTLGHHMLNYFWKFQRDASRLIDFRNRADLSPLGSGAFAGTGFNISRRSTRKDLGFTQQFENSMDAVSSRDLSLEFIF
CLSSIMINLSRLAEELILWSTKEFDFIELDDAFATGSSIMPQKKNPDVPELIRGKTGRVVGHLTALATTYKGLPMAYNKD
FQEDKEGLFDSLDTVESSLKLTSKILSTMTIKTKNMEKALYQDFSNATDIADYLATQGIPFRDAHAVVGQLVKHCQEHNK
LFYQLTEQELDISFKQIADSLEENSQQILTQMDTTKILNIMDPIKCVHNRNSRGAPAPEALQFQLGQAQKYFHSLTEQVK
TYQSFLP

Sequences:

>Translated_487_residues
MSKLWGGRFSKQTDQLVDEFNASIEFDNRLIFYDLLGSQAHVKMLFQQGIIDTTDYKQITEGLDIIWKEAEQDKLEFNLS
DEDIHMSIEKRLIELKGEVGKKLHTARSRNDQVAVDMNMFLCDKAIKLVNELLQNMSVIKELSEQHTKTYMPGYTHLQRA
QPTTLGHHMLNYFWKFQRDASRLIDFRNRADLSPLGSGAFAGTGFNISRRSTRKDLGFTQQFENSMDAVSSRDLSLEFIF
CLSSIMINLSRLAEELILWSTKEFDFIELDDAFATGSSIMPQKKNPDVPELIRGKTGRVVGHLTALATTYKGLPMAYNKD
FQEDKEGLFDSLDTVESSLKLTSKILSTMTIKTKNMEKALYQDFSNATDIADYLATQGIPFRDAHAVVGQLVKHCQEHNK
LFYQLTEQELDISFKQIADSLEENSQQILTQMDTTKILNIMDPIKCVHNRNSRGAPAPEALQFQLGQAQKYFHSLTEQVK
TYQSFLP
>Mature_486_residues
SKLWGGRFSKQTDQLVDEFNASIEFDNRLIFYDLLGSQAHVKMLFQQGIIDTTDYKQITEGLDIIWKEAEQDKLEFNLSD
EDIHMSIEKRLIELKGEVGKKLHTARSRNDQVAVDMNMFLCDKAIKLVNELLQNMSVIKELSEQHTKTYMPGYTHLQRAQ
PTTLGHHMLNYFWKFQRDASRLIDFRNRADLSPLGSGAFAGTGFNISRRSTRKDLGFTQQFENSMDAVSSRDLSLEFIFC
LSSIMINLSRLAEELILWSTKEFDFIELDDAFATGSSIMPQKKNPDVPELIRGKTGRVVGHLTALATTYKGLPMAYNKDF
QEDKEGLFDSLDTVESSLKLTSKILSTMTIKTKNMEKALYQDFSNATDIADYLATQGIPFRDAHAVVGQLVKHCQEHNKL
FYQLTEQELDISFKQIADSLEENSQQILTQMDTTKILNIMDPIKCVHNRNSRGAPAPEALQFQLGQAQKYFHSLTEQVKT
YQSFLP

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=476, Percent_Identity=40.3361344537815, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI68303542, Length=476, Percent_Identity=40.3361344537815, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI68303549, Length=476, Percent_Identity=38.4453781512605, Blast_Score=320, Evalue=3e-87,
Organism=Homo sapiens, GI68303547, Length=476, Percent_Identity=38.0252100840336, Blast_Score=319, Evalue=4e-87,
Organism=Escherichia coli, GI1790398, Length=466, Percent_Identity=42.7038626609442, Blast_Score=367, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321806, Length=392, Percent_Identity=44.3877551020408, Blast_Score=327, Evalue=3e-90,
Organism=Drosophila melanogaster, GI221473854, Length=480, Percent_Identity=38.5416666666667, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI78706858, Length=480, Percent_Identity=38.5416666666667, Blast_Score=333, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_NATTJ (B2A8E3)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917095.1
- ProteinModelPortal:   B2A8E3
- SMR:   B2A8E3
- GeneID:   6314581
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0922
- HOGENOM:   HBG539632
- OMA:   EDIHTVI
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 55672; Mature: 55541

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLWGGRFSKQTDQLVDEFNASIEFDNRLIFYDLLGSQAHVKMLFQQGIIDTTDYKQIT
CCCCCCCCCCHHHHHHHHHHCCCEEECCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHH
EGLDIIWKEAEQDKLEFNLSDEDIHMSIEKRLIELKGEVGKKLHTARSRNDQVAVDMNMF
HHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHH
LCDKAIKLVNELLQNMSVIKELSEQHTKTYMPGYTHLQRAQPTTLGHHMLNYFWKFQRDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
SRLIDFRNRADLSPLGSGAFAGTGFNISRRSTRKDLGFTQQFENSMDAVSSRDLSLEFIF
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHH
CLSSIMINLSRLAEELILWSTKEFDFIELDDAFATGSSIMPQKKNPDVPELIRGKTGRVV
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHCCCCCCCCCCCCCHHHHHCCCCCHHH
GHLTALATTYKGLPMAYNKDFQEDKEGLFDSLDTVESSLKLTSKILSTMTIKTKNMEKAL
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQDFSNATDIADYLATQGIPFRDAHAVVGQLVKHCQEHNKLFYQLTEQELDISFKQIADS
HHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LEENSQQILTQMDTTKILNIMDPIKCVHNRNSRGAPAPEALQFQLGQAQKYFHSLTEQVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TYQSFLP
HHHHHCC
>Mature Secondary Structure 
SKLWGGRFSKQTDQLVDEFNASIEFDNRLIFYDLLGSQAHVKMLFQQGIIDTTDYKQIT
CCCCCCCCCHHHHHHHHHHCCCEEECCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHH
EGLDIIWKEAEQDKLEFNLSDEDIHMSIEKRLIELKGEVGKKLHTARSRNDQVAVDMNMF
HHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHH
LCDKAIKLVNELLQNMSVIKELSEQHTKTYMPGYTHLQRAQPTTLGHHMLNYFWKFQRDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
SRLIDFRNRADLSPLGSGAFAGTGFNISRRSTRKDLGFTQQFENSMDAVSSRDLSLEFIF
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHH
CLSSIMINLSRLAEELILWSTKEFDFIELDDAFATGSSIMPQKKNPDVPELIRGKTGRVV
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHCCCCCCCCCCCCCHHHHHCCCCCHHH
GHLTALATTYKGLPMAYNKDFQEDKEGLFDSLDTVESSLKLTSKILSTMTIKTKNMEKAL
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQDFSNATDIADYLATQGIPFRDAHAVVGQLVKHCQEHNKLFYQLTEQELDISFKQIADS
HHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LEENSQQILTQMDTTKILNIMDPIKCVHNRNSRGAPAPEALQFQLGQAQKYFHSLTEQVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TYQSFLP
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA