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The map label for this gene is kanE [H]

Identifier: 188585521

GI number: 188585521

Start: 941102

End: 942067

Strand: Direct

Name: kanE [H]

Synonym: Nther_0893

Alternate gene names: 188585521

Gene position: 941102-942067 (Clockwise)

Preceding gene: 188585520

Following gene: 188585524

Centisome position: 29.73

GC content: 35.71

Gene sequence:

>966_bases
GTGATTAAAATTAAAGGTTTATATTTTGACAGTAAATTAAAGTATTCGGAAAACATATCGAAACCTGTACCAGGTGCCGG
AGAAGCCTTGATAGAAATAAAACTGGCTTCAGTATGTAATACTGATTTAGAAATTATCAAAGGGTATAAATCATTTCAGG
GTATTTTAGGACATGAGTTTGTAGGAATTGTCAGAGAAGCAGAAACTGAAAACTTAAAAGGAAAAAGAGTCGTGGGGGAT
ATAAATATAGGTTGTAATCAGTGTTCAATGTGTTATAAGGGACTTCCCAATCACTGTACAAATAGGAAAATTCTTGGGAT
GTCAGATAAAAACGGGGCCTTTGCAGACTATATTACTTTACCAGAGAAAAATCTCTATATCGTGCCTGATAATGTAACAG
ATTATGAAGCCGTTTTGGCTGAGCCTTTAGCTGCCGCCTTACAGATCCCCGAACTTTGTCATATAAAACCTACAGATAAA
GTCGTTGTTTTGGGTGATGGTAAATTAGGATATTTAATCACACAGGTGTTGGCTAACACTGCTTGTGATTTAAAGGTAGT
CGGAAAACATAGTAATAAACTCAAGCGATTTGAAAGATACAGTAAAGTAGCTCTAATTAATGATTTAGAAGAAGCAGCTT
GCTATGATATGGTTGTTGATAGTACTGGCAATGAACAAGGTTTGAAACTAGCTCAGAAACTAGTAAAGCCCAGAGGGACC
ATAGTTCTAAAAAGTACTTATTCCAGCGTTGCTGCTTTTAACCCTAGCGATTGGGTTGTTAATGAAATTAATATTGTCGG
CAGTAGATGTGGACCTTTAGATGGGGCATTGAGATTGCTAGCTAAAAAATATATCGATGTAGAGAATATATTAGATAACC
CCTGTTCATTGAAGCACTATAAAGCTGTCCTTTTCTCAAAACAGGATTCAAAACCTGTCTTTGACTTAACAAAAGACTCT
GTTTAA

Upstream 100 bases:

>100_bases
TTTTTAATAACACTGGTCAAAGTGTCTATCCAATTCCTATTAATTCAGGAATCTTACTGGATTTTGTCTTGTAGATAAAA
CACTATAATCTGGAGAGGAT

Downstream 100 bases:

>100_bases
GTTTGGTTGTCGGTAATAGCACTTAACAAAAAATAAATCTAATAGAGATAATCTAGATTGCGCTTAATTTGATGTAACTG
TCTTGAAAAATTGAGTTATC

Product: Alcohol dehydrogenase GroES domain protein

Products: NA

Alternate protein names: DOIA dehydrogenase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MIKIKGLYFDSKLKYSENISKPVPGAGEALIEIKLASVCNTDLEIIKGYKSFQGILGHEFVGIVREAETENLKGKRVVGD
INIGCNQCSMCYKGLPNHCTNRKILGMSDKNGAFADYITLPEKNLYIVPDNVTDYEAVLAEPLAAALQIPELCHIKPTDK
VVVLGDGKLGYLITQVLANTACDLKVVGKHSNKLKRFERYSKVALINDLEEAACYDMVVDSTGNEQGLKLAQKLVKPRGT
IVLKSTYSSVAAFNPSDWVVNEINIVGSRCGPLDGALRLLAKKYIDVENILDNPCSLKHYKAVLFSKQDSKPVFDLTKDS
V

Sequences:

>Translated_321_residues
MIKIKGLYFDSKLKYSENISKPVPGAGEALIEIKLASVCNTDLEIIKGYKSFQGILGHEFVGIVREAETENLKGKRVVGD
INIGCNQCSMCYKGLPNHCTNRKILGMSDKNGAFADYITLPEKNLYIVPDNVTDYEAVLAEPLAAALQIPELCHIKPTDK
VVVLGDGKLGYLITQVLANTACDLKVVGKHSNKLKRFERYSKVALINDLEEAACYDMVVDSTGNEQGLKLAQKLVKPRGT
IVLKSTYSSVAAFNPSDWVVNEINIVGSRCGPLDGALRLLAKKYIDVENILDNPCSLKHYKAVLFSKQDSKPVFDLTKDS
V
>Mature_321_residues
MIKIKGLYFDSKLKYSENISKPVPGAGEALIEIKLASVCNTDLEIIKGYKSFQGILGHEFVGIVREAETENLKGKRVVGD
INIGCNQCSMCYKGLPNHCTNRKILGMSDKNGAFADYITLPEKNLYIVPDNVTDYEAVLAEPLAAALQIPELCHIKPTDK
VVVLGDGKLGYLITQVLANTACDLKVVGKHSNKLKRFERYSKVALINDLEEAACYDMVVDSTGNEQGLKLAQKLVKPRGT
IVLKSTYSSVAAFNPSDWVVNEINIVGSRCGPLDGALRLLAKKYIDVENILDNPCSLKHYKAVLFSKQDSKPVFDLTKDS
V

Specific function: Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD(+) or NADP(+), forming 3-amino-2,3-dideoxy-scyllo- inosose (amino-DOI) [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787863, Length=312, Percent_Identity=28.525641025641, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI226510992, Length=292, Percent_Identity=25.3424657534247, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788075, Length=326, Percent_Identity=27.3006134969325, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788073, Length=320, Percent_Identity=25.3125, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1790045, Length=180, Percent_Identity=32.2222222222222, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788407, Length=319, Percent_Identity=21.6300940438871, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17562878, Length=158, Percent_Identity=27.8481012658228, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=169, Percent_Identity=25.4437869822485, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319621, Length=323, Percent_Identity=27.8637770897833, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319257, Length=334, Percent_Identity=23.0538922155689, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323099, Length=321, Percent_Identity=25.5451713395639, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 35260; Mature: 35260

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIKGLYFDSKLKYSENISKPVPGAGEALIEIKLASVCNTDLEIIKGYKSFQGILGHEF
CEEEEEEEECCCCCHHCCCCCCCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHH
VGIVREAETENLKGKRVVGDINIGCNQCSMCYKGLPNHCTNRKILGMSDKNGAFADYITL
HHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEEC
PEKNLYIVPDNVTDYEAVLAEPLAAALQIPELCHIKPTDKVVVLGDGKLGYLITQVLANT
CCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCC
ACDLKVVGKHSNKLKRFERYSKVALINDLEEAACYDMVVDSTGNEQGLKLAQKLVKPRGT
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCC
IVLKSTYSSVAAFNPSDWVVNEINIVGSRCGPLDGALRLLAKKYIDVENILDNPCSLKHY
EEEEECCCHHEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHCCCCCHHHH
KAVLFSKQDSKPVFDLTKDSV
HHEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKIKGLYFDSKLKYSENISKPVPGAGEALIEIKLASVCNTDLEIIKGYKSFQGILGHEF
CEEEEEEEECCCCCHHCCCCCCCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHH
VGIVREAETENLKGKRVVGDINIGCNQCSMCYKGLPNHCTNRKILGMSDKNGAFADYITL
HHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEEC
PEKNLYIVPDNVTDYEAVLAEPLAAALQIPELCHIKPTDKVVVLGDGKLGYLITQVLANT
CCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCC
ACDLKVVGKHSNKLKRFERYSKVALINDLEEAACYDMVVDSTGNEQGLKLAQKLVKPRGT
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCC
IVLKSTYSSVAAFNPSDWVVNEINIVGSRCGPLDGALRLLAKKYIDVENILDNPCSLKHY
EEEEECCCHHEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHCCCCCHHHH
KAVLFSKQDSKPVFDLTKDSV
HHEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA