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The map label for this gene is amiC [H]

Identifier: 188585513

GI number: 188585513

Start: 932694

End: 933596

Strand: Direct

Name: amiC [H]

Synonym: Nther_0885

Alternate gene names: 188585513

Gene position: 932694-933596 (Clockwise)

Preceding gene: 188585512

Following gene: 188585515

Centisome position: 29.46

GC content: 40.53

Gene sequence:

>903_bases
ATGAAAGGATTAAAGTTAACGATTTCACTGCTAGTTTTTGTACTACTTTTAGCAGCAAGTGGCCCTATTATGGGGAACGA
AGTTAGTGCAGAAGAGTTGGAAGAAGCAGAAGTTGTAGTAGAAACTTTAAATGTCAGATCCGGTCCAGGTTTATCAAACT
CACTCATTGACCAGGTCCATCAAGGAGAAACATATGATGTGTTGGACAAGCAAACTAATGAGTCTGAGAGTTACTATCAA
GATTGGGTAAAAATTGATTTTTCAGGATATGAAGAAGCTTGGGTTAGCCAAGATTATGTAAACATCACTACCCCTGAAGC
TATTGATCCTGAACCTGAGAATAACAGTTATAGACCACTTGAAGGTACTAGAATAGTTCTTGATCCCGGTCACGGTGGAT
GGGACCCTGGTGCTGTAGGGCCGACTGGATTAACTGAAAAGGAAGTAGCCCTTGATGTTTCCTTCAAAACTCAAGATAAG
CTTGAAAGCTTAGGCGCTGAGGTTTATTTAACTAGGGAAAGCGATATAGATATTCCACTTGCAAACAGAGCCTACTTTGC
CAATGATTTATGGGCAGACCTATTTATTAGTATTCATGCTAACGGTGCTATCAATCGAGGAGCACAGGGTACAGAAACTC
ACTACTCTTCATGGAGAAATCCCAATGACTACTTTTTAGCTGAAAGCCTGCAGGATAGCATGATAGATAACATCAACAGA
GTCGATCGAGGCATCATTGACAGCAATTTTGCCGTGTTAACCCATGCAAGAATGCCGGCAGCTTTGGTTGAACTTGCTTT
TATAAGCAATTATGAAGAGGAACGATTATTGGGATCAGATTACTTTCAAGAGAATGCGGCACAGGGGATTACGGAAGGTA
TCGTAGACTACTTTAATTATTAA

Upstream 100 bases:

>100_bases
GTATTATAATAGAACTTGTATCTAGTTAACATAACTTGCTCAATCAAAAAATATACAAGAGCAAGTGAACAAATCAGAAC
ATTGGAGGGGGATAAAAGTA

Downstream 100 bases:

>100_bases
AACAACATTATAAACAAAATTATAATAAGTAACCAATAAATAACACCAACAGGAAGGCTGTCCTTAATGTAATTTTGGAC
AGCCTTCCTGTTTAATTAAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKGLKLTISLLVFVLLLAASGPIMGNEVSAEELEEAEVVVETLNVRSGPGLSNSLIDQVHQGETYDVLDKQTNESESYYQ
DWVKIDFSGYEEAWVSQDYVNITTPEAIDPEPENNSYRPLEGTRIVLDPGHGGWDPGAVGPTGLTEKEVALDVSFKTQDK
LESLGAEVYLTRESDIDIPLANRAYFANDLWADLFISIHANGAINRGAQGTETHYSSWRNPNDYFLAESLQDSMIDNINR
VDRGIIDSNFAVLTHARMPAALVELAFISNYEEERLLGSDYFQENAAQGITEGIVDYFNY

Sequences:

>Translated_300_residues
MKGLKLTISLLVFVLLLAASGPIMGNEVSAEELEEAEVVVETLNVRSGPGLSNSLIDQVHQGETYDVLDKQTNESESYYQ
DWVKIDFSGYEEAWVSQDYVNITTPEAIDPEPENNSYRPLEGTRIVLDPGHGGWDPGAVGPTGLTEKEVALDVSFKTQDK
LESLGAEVYLTRESDIDIPLANRAYFANDLWADLFISIHANGAINRGAQGTETHYSSWRNPNDYFLAESLQDSMIDNINR
VDRGIIDSNFAVLTHARMPAALVELAFISNYEEERLLGSDYFQENAAQGITEGIVDYFNY
>Mature_300_residues
MKGLKLTISLLVFVLLLAASGPIMGNEVSAEELEEAEVVVETLNVRSGPGLSNSLIDQVHQGETYDVLDKQTNESESYYQ
DWVKIDFSGYEEAWVSQDYVNITTPEAIDPEPENNSYRPLEGTRIVLDPGHGGWDPGAVGPTGLTEKEVALDVSFKTQDK
LESLGAEVYLTRESDIDIPLANRAYFANDLWADLFISIHANGAINRGAQGTETHYSSWRNPNDYFLAESLQDSMIDNINR
VDRGIIDSNFAVLTHARMPAALVELAFISNYEEERLLGSDYFQENAAQGITEGIVDYFNY

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=281, Percent_Identity=29.8932384341637, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082163, Length=239, Percent_Identity=33.0543933054393, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1790611, Length=223, Percent_Identity=30.9417040358744, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 33329; Mature: 33329

Theoretical pI: Translated: 3.94; Mature: 3.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLKLTISLLVFVLLLAASGPIMGNEVSAEELEEAEVVVETLNVRSGPGLSNSLIDQVH
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
QGETYDVLDKQTNESESYYQDWVKIDFSGYEEAWVSQDYVNITTPEAIDPEPENNSYRPL
CCCCHHHHCCCCCCCHHHHHHHEEEECCCCHHHHCCCCCEEECCCCCCCCCCCCCCCCCC
EGTRIVLDPGHGGWDPGAVGPTGLTEKEVALDVSFKTQDKLESLGAEVYLTRESDIDIPL
CCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCEEEEEECCCCCCCC
ANRAYFANDLWADLFISIHANGAINRGAQGTETHYSSWRNPNDYFLAESLQDSMIDNINR
CCCHHHHHHHHEEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VDRGIIDSNFAVLTHARMPAALVELAFISNYEEERLLGSDYFQENAAQGITEGIVDYFNY
HHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGLKLTISLLVFVLLLAASGPIMGNEVSAEELEEAEVVVETLNVRSGPGLSNSLIDQVH
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
QGETYDVLDKQTNESESYYQDWVKIDFSGYEEAWVSQDYVNITTPEAIDPEPENNSYRPL
CCCCHHHHCCCCCCCHHHHHHHEEEECCCCHHHHCCCCCEEECCCCCCCCCCCCCCCCCC
EGTRIVLDPGHGGWDPGAVGPTGLTEKEVALDVSFKTQDKLESLGAEVYLTRESDIDIPL
CCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCEEEEEECCCCCCCC
ANRAYFANDLWADLFISIHANGAINRGAQGTETHYSSWRNPNDYFLAESLQDSMIDNINR
CCCHHHHHHHHEEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VDRGIIDSNFAVLTHARMPAALVELAFISNYEEERLLGSDYFQENAAQGITEGIVDYFNY
HHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]