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The map label for this gene is mcrB [H]

Identifier: 188585434

GI number: 188585434

Start: 838950

End: 840458

Strand: Direct

Name: mcrB [H]

Synonym: Nther_0805

Alternate gene names: 188585434

Gene position: 838950-840458 (Clockwise)

Preceding gene: 188585433

Following gene: 188585435

Centisome position: 26.5

GC content: 31.21

Gene sequence:

>1509_bases
GTGAATCCATTAAAATACAAAAGAAAAGTCCAAAACATAAACAATGTAGTGCTACCTAAATTAGTAGGTAATTATCAATA
CAATGTTTATTTCAAGGGATATAATAAATCAAAGGAAGATTTTATAGAGGAAAATCCAGAATATGGGGGTGGCTCAGGGC
ATCCTGGAGTAGATCTGGATAAGTTCTCTAACGATTTAGCTAACTTGTTAAATGCAGATGGCGATTCATTTCAAGAAGCT
TATATTCAATTTAGTAAACATATTGGTGTTGGTAAGGCCATTATATCTGGCTATTTGCACTTATATGATCCTAATAAGTT
TCCGTTAATAAATGGTGCGTCAATAAGTGGTATTGAAAAATACATAGGTAAACAAGATCATCTGGCATTGAAAAACTATG
CTGAAAAAGAGAGGAAAAGACAAAATATTACCCAGCCAATTAATAATTCTGATTTTAGAAATTATCTTGCTTATTATAAT
TTGTTAAAGGAATTGTTAACTTTGGAAGAACTGAAAAACTATCATTATGTAGATGCTTTCCTCTGGTATGTCAGTAAATA
CGGCAAACCTGATGCTAGCGATGTTACTGTGCCAACAGGAGCAATAACCGAAGTAGTTGAAGAACAAGATGGGGAAGCTC
CATTTGAACAATCAATAAGTAATTTAACTGAGGAGATTAGTTTTGAGCAAGATGTTACAGAAGAAATCTTGAACTTACTT
AAGTTGAAGAAAAATGTTGTATTCTACGGACCACCAGGAACAGGAAAGACGTATGTAGCCAAAAAAATTGCTAATTACTT
AGTTGGGGGTCAATCTAAAAATATTAAATTTATTCAATTTCATCAGAACTATTCTTATGAGGATTTTATAGAAGGAATCA
GACCTGAATCAAAGAAGGTAAATGGAACTCATATTATTGACTATCCTATTAGACCTGGAGTTTTCAAAACCTTATGTGAC
AATGCCAAAGAAAACCCTGAGGAAAAATATGTACTCATTATCGACGAATTTAACAGAGGTAATATTTCTAAAATATTTGG
TGAACTGTTGTATTCTTTAGAATACAGATCTGAAGATAATTCAATTCAACTACCTTATTCAAAGGAAAAAGAGTTATTTT
ATATACCCGATAATCTTTATATTATAGCCACAATGAATACAGCGGATAAATCCTTGACTAGGATTGACTTTGCAATGAGA
AGAAGATTTGCCTTCTATAAATTTAACGTAGATACTAAGATATTGATTAATTGGGGTGAAAAACAAGGTCTAGTCATGGA
CTCTCTGGCAACTTTAATTGAAGATGTAAATAATGAAATAGGAGATGAAAATTTCTTTATTGGAATATCCTTTTTTATGA
GAGAAGACTTACCCGAGACTATTAAATACATTTGGAAATCTGAAATTTATCCTTATTTAGAAGAGTACTTCATTGATGAT
ATGAATTCAAATATTGATAAATTCAAATGGGAAAATGTCAAGCATAGATTAAAGGAATTGATAGAATGA

Upstream 100 bases:

>100_bases
ATGGAGCTAGAAAGAAGGGAAAATGCCGAAAGGGAAATGCGAGAACTAATAGAAAAGACTAATTTACTTCATGGAGGTAT
CTGGACAAGCGAGCACTTAT

Downstream 100 bases:

>100_bases
TGGATAATACTCTAAAGCTAAAAGAGTATGATGAAAGTAATAAACTTCAATTAAGACAGGACCATCTGGATTTATTACTA
GCTAAATTTGATAATCAACT

Product: ATPase associated with various cellular activities AAA_5

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MNPLKYKRKVQNINNVVLPKLVGNYQYNVYFKGYNKSKEDFIEENPEYGGGSGHPGVDLDKFSNDLANLLNADGDSFQEA
YIQFSKHIGVGKAIISGYLHLYDPNKFPLINGASISGIEKYIGKQDHLALKNYAEKERKRQNITQPINNSDFRNYLAYYN
LLKELLTLEELKNYHYVDAFLWYVSKYGKPDASDVTVPTGAITEVVEEQDGEAPFEQSISNLTEEISFEQDVTEEILNLL
KLKKNVVFYGPPGTGKTYVAKKIANYLVGGQSKNIKFIQFHQNYSYEDFIEGIRPESKKVNGTHIIDYPIRPGVFKTLCD
NAKENPEEKYVLIIDEFNRGNISKIFGELLYSLEYRSEDNSIQLPYSKEKELFYIPDNLYIIATMNTADKSLTRIDFAMR
RRFAFYKFNVDTKILINWGEKQGLVMDSLATLIEDVNNEIGDENFFIGISFFMREDLPETIKYIWKSEIYPYLEEYFIDD
MNSNIDKFKWENVKHRLKELIE

Sequences:

>Translated_502_residues
MNPLKYKRKVQNINNVVLPKLVGNYQYNVYFKGYNKSKEDFIEENPEYGGGSGHPGVDLDKFSNDLANLLNADGDSFQEA
YIQFSKHIGVGKAIISGYLHLYDPNKFPLINGASISGIEKYIGKQDHLALKNYAEKERKRQNITQPINNSDFRNYLAYYN
LLKELLTLEELKNYHYVDAFLWYVSKYGKPDASDVTVPTGAITEVVEEQDGEAPFEQSISNLTEEISFEQDVTEEILNLL
KLKKNVVFYGPPGTGKTYVAKKIANYLVGGQSKNIKFIQFHQNYSYEDFIEGIRPESKKVNGTHIIDYPIRPGVFKTLCD
NAKENPEEKYVLIIDEFNRGNISKIFGELLYSLEYRSEDNSIQLPYSKEKELFYIPDNLYIIATMNTADKSLTRIDFAMR
RRFAFYKFNVDTKILINWGEKQGLVMDSLATLIEDVNNEIGDENFFIGISFFMREDLPETIKYIWKSEIYPYLEEYFIDD
MNSNIDKFKWENVKHRLKELIE
>Mature_502_residues
MNPLKYKRKVQNINNVVLPKLVGNYQYNVYFKGYNKSKEDFIEENPEYGGGSGHPGVDLDKFSNDLANLLNADGDSFQEA
YIQFSKHIGVGKAIISGYLHLYDPNKFPLINGASISGIEKYIGKQDHLALKNYAEKERKRQNITQPINNSDFRNYLAYYN
LLKELLTLEELKNYHYVDAFLWYVSKYGKPDASDVTVPTGAITEVVEEQDGEAPFEQSISNLTEEISFEQDVTEEILNLL
KLKKNVVFYGPPGTGKTYVAKKIANYLVGGQSKNIKFIQFHQNYSYEDFIEGIRPESKKVNGTHIIDYPIRPGVFKTLCD
NAKENPEEKYVLIIDEFNRGNISKIFGELLYSLEYRSEDNSIQLPYSKEKELFYIPDNLYIIATMNTADKSLTRIDFAMR
RRFAFYKFNVDTKILINWGEKQGLVMDSLATLIEDVNNEIGDENFFIGISFFMREDLPETIKYIWKSEIYPYLEEYFIDD
MNSNIDKFKWENVKHRLKELIE

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=272, Percent_Identity=41.9117647058824, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 58282; Mature: 58282

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLKYKRKVQNINNVVLPKLVGNYQYNVYFKGYNKSKEDFIEENPEYGGGSGHPGVDLD
CCCHHHHHHHHHCCCEEHHHHHCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCHH
KFSNDLANLLNADGDSFQEAYIQFSKHIGVGKAIISGYLHLYDPNKFPLINGASISGIEK
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHCEEEEECCCCCCEECCCCHHHHHH
YIGKQDHLALKNYAEKERKRQNITQPINNSDFRNYLAYYNLLKELLTLEELKNYHYVDAF
HCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LWYVSKYGKPDASDVTVPTGAITEVVEEQDGEAPFEQSISNLTEEISFEQDVTEEILNLL
HHHHHHCCCCCCCCEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
KLKKNVVFYGPPGTGKTYVAKKIANYLVGGQSKNIKFIQFHQNYSYEDFIEGIRPESKKV
HHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCC
NGTHIIDYPIRPGVFKTLCDNAKENPEEKYVLIIDEFNRGNISKIFGELLYSLEYRSEDN
CCCEEEECCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
SIQLPYSKEKELFYIPDNLYIIATMNTADKSLTRIDFAMRRRFAFYKFNVDTKILINWGE
CEECCCCCCCCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHEEEEECCCEEEEEEECC
KQGLVMDSLATLIEDVNNEIGDENFFIGISFFMREDLPETIKYIWKSEIYPYLEEYFIDD
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
MNSNIDKFKWENVKHRLKELIE
CCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPLKYKRKVQNINNVVLPKLVGNYQYNVYFKGYNKSKEDFIEENPEYGGGSGHPGVDLD
CCCHHHHHHHHHCCCEEHHHHHCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCHH
KFSNDLANLLNADGDSFQEAYIQFSKHIGVGKAIISGYLHLYDPNKFPLINGASISGIEK
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHCEEEEECCCCCCEECCCCHHHHHH
YIGKQDHLALKNYAEKERKRQNITQPINNSDFRNYLAYYNLLKELLTLEELKNYHYVDAF
HCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LWYVSKYGKPDASDVTVPTGAITEVVEEQDGEAPFEQSISNLTEEISFEQDVTEEILNLL
HHHHHHCCCCCCCCEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
KLKKNVVFYGPPGTGKTYVAKKIANYLVGGQSKNIKFIQFHQNYSYEDFIEGIRPESKKV
HHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCC
NGTHIIDYPIRPGVFKTLCDNAKENPEEKYVLIIDEFNRGNISKIFGELLYSLEYRSEDN
CCCEEEECCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
SIQLPYSKEKELFYIPDNLYIIATMNTADKSLTRIDFAMRRRFAFYKFNVDTKILINWGE
CEECCCCCCCCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHEEEEECCCEEEEEEECC
KQGLVMDSLATLIEDVNNEIGDENFFIGISFFMREDLPETIKYIWKSEIYPYLEEYFIDD
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
MNSNIDKFKWENVKHRLKELIE
CCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]