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The map label for this gene is metK [H]

Identifier: 188585311

GI number: 188585311

Start: 700097

End: 701302

Strand: Reverse

Name: metK [H]

Synonym: Nther_0675

Alternate gene names: 188585311

Gene position: 701302-700097 (Counterclockwise)

Preceding gene: 188585320

Following gene: 188585309

Centisome position: 22.15

GC content: 38.97

Gene sequence:

>1206_bases
TTGGCAGTTGAAAAAACTTCGGAAATTAAAGCCAAAGAAGAAAATAAGAATTTAATTACTTCTGAATCGGTGACTGAGGG
ACATCCAGATAAGATATGTGATCAAATTTCAGATGCAGTCTTAGATGCCTTATTGGAGGAGGATCCAGAATCTAGAGTTG
CTTGTGAGACTGCTGTCACTACTGGACAAGTGAATGTATTCGGTGAAATCACCACTAAGGCTAATGTGGACATAAGTAAT
GTGGTTAGAGAAACTCTTAAAGATATAGGTTACACCAAAGCTAAATACGGATTCGACTGCGATACATGTGGAGTTTCTGT
TTCAATTGATGAACAATCTCCTGATATTGCAATGGGTGTGGATGGCGAAGATAAAAATGATGTTGGTGCTGGTGATCAAG
GCATGATGTATGGTTATGCCACTGACGAAACTGAAGAATTCATGCCTATGCCAATCATGCTTGCTCACAGGCTATCTAGG
AAGCTATCAGAAGCTAGAAGAAATGGAGAGATTTCTTATCTACGTCCTGATGGAAAAACACAGGTGACTGTGGAATATGA
AAACAATCAACCTAGCGCAATTTCTAATATCTTAATTTCCGCTCAACATCCAGAAGAAGTTGATAATGAAACTATCGAAA
ATGATTTGATTGAAAAAATTATCAAAAGAAATGTCCCTGAAAACTTAATAACTGATGAAACCGAGTTTTTAATTAATCCT
ACTGGAAGGTTTGTAGTTGGTGGCCCTCAAGGGGATGCAGGGGTTACAGGAAGGAAAATAGTTGTCGATACCTATGGTGG
TATTGGCAGAATTGGAGGAGGTGCCTTCTCCGGAAAGGATCCATCTAAGGTAGACCGTTCAGCAGCTTATGCGGCTAGAT
ATGCTGCCAAAAATGTTGTAGCTGCTGGATTAGCAAATAGATGTGAAGTATCAGTTGCATATTCCATCGGTGTAGCCAAA
CCGGTATCCTTATGGGTGAATACCTTTGGAACAGGAAAAGTTTCTGAAGAAAAACTATCCAATTGGTTGCAAGAAAGCTT
TGACTGGCGTCCAGCTGCCATTATAGATAGATTCGAGTTAAAACGTCCTATTTACAGACAAACTGCTGCATACGGACATT
TTGGCAGAAACGATTTAAATTTACCTTGGGAAAAGCTGGATATGGTCAAAGATTTAAAAAATAAAGAGTCTGTTAAGTTG
TTATGA

Upstream 100 bases:

>100_bases
CAGTTTAGGTTAGCAGTACTTTAACTACTGAAAGTAGTTACCTTGAAAGTGGCACCTGTAAAGTAACAGTTACAAAAACT
TATTCTAGGAGGTGGATTTT

Downstream 100 bases:

>100_bases
AAAATAAGCTCACTTGCTCGCAAGATTTCAAAAGATTTGGCAGTCAAGCGGCATTATTACTATGTAAAAGCCAAAAAGTC
CTATTACGATTAGAGTAATT

Product: methionine adenosyltransferase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAVEKTSEIKAKEENKNLITSESVTEGHPDKICDQISDAVLDALLEEDPESRVACETAVTTGQVNVFGEITTKANVDISN
VVRETLKDIGYTKAKYGFDCDTCGVSVSIDEQSPDIAMGVDGEDKNDVGAGDQGMMYGYATDETEEFMPMPIMLAHRLSR
KLSEARRNGEISYLRPDGKTQVTVEYENNQPSAISNILISAQHPEEVDNETIENDLIEKIIKRNVPENLITDETEFLINP
TGRFVVGGPQGDAGVTGRKIVVDTYGGIGRIGGGAFSGKDPSKVDRSAAYAARYAAKNVVAAGLANRCEVSVAYSIGVAK
PVSLWVNTFGTGKVSEEKLSNWLQESFDWRPAAIIDRFELKRPIYRQTAAYGHFGRNDLNLPWEKLDMVKDLKNKESVKL
L

Sequences:

>Translated_401_residues
MAVEKTSEIKAKEENKNLITSESVTEGHPDKICDQISDAVLDALLEEDPESRVACETAVTTGQVNVFGEITTKANVDISN
VVRETLKDIGYTKAKYGFDCDTCGVSVSIDEQSPDIAMGVDGEDKNDVGAGDQGMMYGYATDETEEFMPMPIMLAHRLSR
KLSEARRNGEISYLRPDGKTQVTVEYENNQPSAISNILISAQHPEEVDNETIENDLIEKIIKRNVPENLITDETEFLINP
TGRFVVGGPQGDAGVTGRKIVVDTYGGIGRIGGGAFSGKDPSKVDRSAAYAARYAAKNVVAAGLANRCEVSVAYSIGVAK
PVSLWVNTFGTGKVSEEKLSNWLQESFDWRPAAIIDRFELKRPIYRQTAAYGHFGRNDLNLPWEKLDMVKDLKNKESVKL
L
>Mature_400_residues
AVEKTSEIKAKEENKNLITSESVTEGHPDKICDQISDAVLDALLEEDPESRVACETAVTTGQVNVFGEITTKANVDISNV
VRETLKDIGYTKAKYGFDCDTCGVSVSIDEQSPDIAMGVDGEDKNDVGAGDQGMMYGYATDETEEFMPMPIMLAHRLSRK
LSEARRNGEISYLRPDGKTQVTVEYENNQPSAISNILISAQHPEEVDNETIENDLIEKIIKRNVPENLITDETEFLINPT
GRFVVGGPQGDAGVTGRKIVVDTYGGIGRIGGGAFSGKDPSKVDRSAAYAARYAAKNVVAAGLANRCEVSVAYSIGVAKP
VSLWVNTFGTGKVSEEKLSNWLQESFDWRPAAIIDRFELKRPIYRQTAAYGHFGRNDLNLPWEKLDMVKDLKNKESVKLL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=377, Percent_Identity=55.9681697612732, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI4557737, Length=376, Percent_Identity=54.7872340425532, Blast_Score=405, Evalue=1e-113,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=57.5520833333333, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17538498, Length=377, Percent_Identity=56.763925729443, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=390, Percent_Identity=56.1538461538462, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17538494, Length=377, Percent_Identity=55.4376657824934, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17551082, Length=381, Percent_Identity=53.0183727034121, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32565905, Length=377, Percent_Identity=50.3978779840849, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=54.8780487804878, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=53.9589442815249, Blast_Score=350, Evalue=9e-97,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=53.8205980066445, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI32565909, Length=85, Percent_Identity=57.6470588235294, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=42, Percent_Identity=71.4285714285714, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=55.026455026455, Blast_Score=425, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320710, Length=373, Percent_Identity=54.4235924932976, Blast_Score=416, Evalue=1e-117,
Organism=Drosophila melanogaster, GI45552159, Length=373, Percent_Identity=53.0831099195711, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580529, Length=373, Percent_Identity=53.0831099195711, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580537, Length=373, Percent_Identity=53.0831099195711, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580533, Length=373, Percent_Identity=53.0831099195711, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580535, Length=373, Percent_Identity=53.0831099195711, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580531, Length=373, Percent_Identity=53.0831099195711, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580527, Length=373, Percent_Identity=53.3512064343164, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=373, Percent_Identity=53.3512064343164, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=51.2, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=58.1196581196581, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43969; Mature: 43838

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEKTSEIKAKEENKNLITSESVTEGHPDKICDQISDAVLDALLEEDPESRVACETAVT
CCCCCCHHHHCHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHEE
TGQVNVFGEITTKANVDISNVVRETLKDIGYTKAKYGFDCDTCGVSVSIDEQSPDIAMGV
CCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEC
DGEDKNDVGAGDQGMMYGYATDETEEFMPMPIMLAHRLSRKLSEARRNGEISYLRPDGKT
CCCCCCCCCCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCE
QVTVEYENNQPSAISNILISAQHPEEVDNETIENDLIEKIIKRNVPENLITDETEFLINP
EEEEEECCCCCHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEECC
TGRFVVGGPQGDAGVTGRKIVVDTYGGIGRIGGGAFSGKDPSKVDRSAAYAARYAAKNVV
CCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAGLANRCEVSVAYSIGVAKPVSLWVNTFGTGKVSEEKLSNWLQESFDWRPAAIIDRFEL
HHCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
KRPIYRQTAAYGHFGRNDLNLPWEKLDMVKDLKNKESVKLL
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEECC
>Mature Secondary Structure 
AVEKTSEIKAKEENKNLITSESVTEGHPDKICDQISDAVLDALLEEDPESRVACETAVT
CCCCCHHHHCHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHEE
TGQVNVFGEITTKANVDISNVVRETLKDIGYTKAKYGFDCDTCGVSVSIDEQSPDIAMGV
CCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEC
DGEDKNDVGAGDQGMMYGYATDETEEFMPMPIMLAHRLSRKLSEARRNGEISYLRPDGKT
CCCCCCCCCCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCE
QVTVEYENNQPSAISNILISAQHPEEVDNETIENDLIEKIIKRNVPENLITDETEFLINP
EEEEEECCCCCHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEECC
TGRFVVGGPQGDAGVTGRKIVVDTYGGIGRIGGGAFSGKDPSKVDRSAAYAARYAAKNVV
CCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAGLANRCEVSVAYSIGVAKPVSLWVNTFGTGKVSEEKLSNWLQESFDWRPAAIIDRFEL
HHCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
KRPIYRQTAAYGHFGRNDLNLPWEKLDMVKDLKNKESVKLL
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA