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The map label for this gene is ycjI [C]

Identifier: 188585295

GI number: 188585295

Start: 681222

End: 682361

Strand: Direct

Name: ycjI [C]

Synonym: Nther_0658

Alternate gene names: 188585295

Gene position: 681222-682361 (Clockwise)

Preceding gene: 188585294

Following gene: 188585296

Centisome position: 21.52

GC content: 35.35

Gene sequence:

>1140_bases
ATGAATGTATTCAATATTAAAAAAAACCATAAAATCATGCTCATTTTTCTTGTTTTAATAATGCTTAGTTTAATTATTAC
TTTTCTTCTCGGTCGGGATGACTTAAATGATGAAATCCATGATACGAAAAAAAATGAAGAAAAGCGAGATGGTGCTGACA
AGGACTATACTGAGTTACCATCATGGCAAACTATTGACCCCCAAGACTTAGATTATGTGGTTGATCCCTATCAGACCTAT
ACTTATGACCAAATGGAACAGGATTTGGACCAATTAAAAGAAAATTATGGTGATTTGTTAGAAGTTAGGACAATAGGAAA
ATCAGTCCTGGGGAAAGATTTATATGCAGTAAGGATAGGGAATGAGGACAACACAATTTTAGTGGAAGCCAGTCATCATG
CCAGGGAATGGATCTCCACTAATTTAACAATGAAAAAATTGGATCGTTATGCTTATAAATACGTCCAAAACGAAAAGATA
GGTGAGTTTAATATTAGAGAAATTCTGGACGATGTGGGAATTTGGTTTGTCCCAATGGTCAACCCTGATGGTGTAACATT
ACATCAAAAAGGGCTTGATGGGATTCCTGAAGAATATCATGACGAATTTGTAGACTATAATGTAGACTGGGCTGGAGAAG
ATTTTTATTTATGGAAAGCAAATATTAATGGAGTTGACTTAAATAGGCAGTATCCTGCGGATTGGGAAGACTCCGATTGT
ACTGGTGTTAGATTTGCTATGAATTATAAAGGAGAGGAACCTTTATCAGAACCTGAATCTCAAGCTCTATATGAGTTTAC
TAAAGAAACCAAACCTTTAAGTGCTATATCATATCACACTCGAGGGCAAGTGATTTTCTGGTATTACCATAACAAACATT
TGGAAAGGGATCAGGAAATTGGTGATCAGCTCAGCGAATTAACAGGTTATACATTATATCCTCCTAGACCCGATTACAGA
GGTGCAAATTTCAATGACTGGTTTATCCAAAAATTTGATAGGCCAGCTTATATTTTAGAATTAGTGCCTTTCACAGGTGA
CGAGGAAGATCGTGATTTGGATTATCTTAAGACTGAATGGAAAAATAATCGAGAGGTAGCGGTGTTTTTGGCAAAAGTTG
CTAATAAAAAGTTAAAATAA

Upstream 100 bases:

>100_bases
TAAAGACTTTTGGGATAACCCTTTTTTTGTAATATAAAAGCTAGTTCATTGTATACTTTGATAATAGACAAATAAGATTT
TATAGGGGTGAATTGTTTAT

Downstream 100 bases:

>100_bases
CAAAATGTTTCAGTAATAGTAATTTTTTAGAAGGAATCACCTAAAATAAGGTCGAATTTAACATTGGAGGTGATTCCATG
AGACCTAAAGCCATAGCTAT

Product: peptidase M14 carboxypeptidase A

Products: NA

Alternate protein names: Endopeptidase I; Gamma-D-glutamyl-L-diamino acid endopeptidase I; Gamma-D-glutamyl-meso-diaminopimelate peptidase I [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MNVFNIKKNHKIMLIFLVLIMLSLIITFLLGRDDLNDEIHDTKKNEEKRDGADKDYTELPSWQTIDPQDLDYVVDPYQTY
TYDQMEQDLDQLKENYGDLLEVRTIGKSVLGKDLYAVRIGNEDNTILVEASHHAREWISTNLTMKKLDRYAYKYVQNEKI
GEFNIREILDDVGIWFVPMVNPDGVTLHQKGLDGIPEEYHDEFVDYNVDWAGEDFYLWKANINGVDLNRQYPADWEDSDC
TGVRFAMNYKGEEPLSEPESQALYEFTKETKPLSAISYHTRGQVIFWYYHNKHLERDQEIGDQLSELTGYTLYPPRPDYR
GANFNDWFIQKFDRPAYILELVPFTGDEEDRDLDYLKTEWKNNREVAVFLAKVANKKLK

Sequences:

>Translated_379_residues
MNVFNIKKNHKIMLIFLVLIMLSLIITFLLGRDDLNDEIHDTKKNEEKRDGADKDYTELPSWQTIDPQDLDYVVDPYQTY
TYDQMEQDLDQLKENYGDLLEVRTIGKSVLGKDLYAVRIGNEDNTILVEASHHAREWISTNLTMKKLDRYAYKYVQNEKI
GEFNIREILDDVGIWFVPMVNPDGVTLHQKGLDGIPEEYHDEFVDYNVDWAGEDFYLWKANINGVDLNRQYPADWEDSDC
TGVRFAMNYKGEEPLSEPESQALYEFTKETKPLSAISYHTRGQVIFWYYHNKHLERDQEIGDQLSELTGYTLYPPRPDYR
GANFNDWFIQKFDRPAYILELVPFTGDEEDRDLDYLKTEWKNNREVAVFLAKVANKKLK
>Mature_379_residues
MNVFNIKKNHKIMLIFLVLIMLSLIITFLLGRDDLNDEIHDTKKNEEKRDGADKDYTELPSWQTIDPQDLDYVVDPYQTY
TYDQMEQDLDQLKENYGDLLEVRTIGKSVLGKDLYAVRIGNEDNTILVEASHHAREWISTNLTMKKLDRYAYKYVQNEKI
GEFNIREILDDVGIWFVPMVNPDGVTLHQKGLDGIPEEYHDEFVDYNVDWAGEDFYLWKANINGVDLNRQYPADWEDSDC
TGVRFAMNYKGEEPLSEPESQALYEFTKETKPLSAISYHTRGQVIFWYYHNKHLERDQEIGDQLSELTGYTLYPPRPDYR
GANFNDWFIQKFDRPAYILELVPFTGDEEDRDLDYLKTEWKNNREVAVFLAKVANKKLK

Specific function: An endopeptidase which hydrolyzes the gamma-D-Glu- (L)meso-diaminopimelic acid bond of L-Ala-gamma-D-Glu-(L)meso- diaminopimelic acid and L-Ala-gamma-D-Glu-(L)meso-diaminopimelic acid(L)-D-Ala peptides. It is active on spore cortex peptidoglycan [H]

COG id: COG2866

COG function: function code E; Predicted carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Caenorhabditis elegans, GI25146607, Length=294, Percent_Identity=24.1496598639456, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI193207630, Length=293, Percent_Identity=26.2798634812287, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193203290, Length=237, Percent_Identity=21.9409282700422, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71990304, Length=225, Percent_Identity=24, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71990310, Length=225, Percent_Identity=24, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6321924, Length=258, Percent_Identity=25.5813953488372, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24639964, Length=228, Percent_Identity=25, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000834
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01476 LysM; PF00246 Peptidase_M14 [H]

EC number: =3.4.19.11 [H]

Molecular weight: Translated: 44656; Mature: 44656

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVFNIKKNHKIMLIFLVLIMLSLIITFLLGRDDLNDEIHDTKKNEEKRDGADKDYTELP
CCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHCCCCCCHHHCC
SWQTIDPQDLDYVVDPYQTYTYDQMEQDLDQLKENYGDLLEVRTIGKSVLGKDLYAVRIG
CCCCCCCCCCCEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
NEDNTILVEASHHAREWISTNLTMKKLDRYAYKYVQNEKIGEFNIREILDDVGIWFVPMV
CCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEEE
NPDGVTLHQKGLDGIPEEYHDEFVDYNVDWAGEDFYLWKANINGVDLNRQYPADWEDSDC
CCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCC
TGVRFAMNYKGEEPLSEPESQALYEFTKETKPLSAISYHTRGQVIFWYYHNKHLERDQEI
CEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHEEECCCCCEEEEEECCCCCCHHHHH
GDQLSELTGYTLYPPRPDYRGANFNDWFIQKFDRPAYILELVPFTGDEEDRDLDYLKTEW
HHHHHHHCCCEECCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHC
KNNREVAVFLAKVANKKLK
CCCCCCEEHEEHHHCCCCC
>Mature Secondary Structure
MNVFNIKKNHKIMLIFLVLIMLSLIITFLLGRDDLNDEIHDTKKNEEKRDGADKDYTELP
CCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHCCCCCCHHHCC
SWQTIDPQDLDYVVDPYQTYTYDQMEQDLDQLKENYGDLLEVRTIGKSVLGKDLYAVRIG
CCCCCCCCCCCEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
NEDNTILVEASHHAREWISTNLTMKKLDRYAYKYVQNEKIGEFNIREILDDVGIWFVPMV
CCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEEE
NPDGVTLHQKGLDGIPEEYHDEFVDYNVDWAGEDFYLWKANINGVDLNRQYPADWEDSDC
CCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCC
TGVRFAMNYKGEEPLSEPESQALYEFTKETKPLSAISYHTRGQVIFWYYHNKHLERDQEI
CEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHEEECCCCCEEEEEECCCCCCHHHHH
GDQLSELTGYTLYPPRPDYRGANFNDWFIQKFDRPAYILELVPFTGDEEDRDLDYLKTEW
HHHHHHHCCCEECCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHC
KNNREVAVFLAKVANKKLK
CCCCCCEEHEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8503890; 3922755 [H]