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The map label for this gene is hmeA [H]

Identifier: 188585282

GI number: 188585282

Start: 667861

End: 668550

Strand: Direct

Name: hmeA [H]

Synonym: Nther_0644

Alternate gene names: 188585282

Gene position: 667861-668550 (Clockwise)

Preceding gene: 188585281

Following gene: 188585283

Centisome position: 21.1

GC content: 36.67

Gene sequence:

>690_bases
TTGACTAAATATGCTATGGTTATCGACTTGCACCGATGTACAGGTTGTGGTGCATGTGCTATTACGTGTAAAATAGAAAA
CAATGTCCCTAGAGGATTTCGCTGGGCTGATTATATTCATGAAACAAAAGGAGAATTTCCCAATGTAACGTATACTTATA
TTCCCACCTTGTGTAACCATTGTGATCACGCTCCCTGTGTGGAAGTATGTCCAACAGATCCTAAAGCTATGTATAAGACG
GAACACGGCTTGACCTTGCACGATTCAAAAGAATGTATTGGGTGCCGTCAATGTGAAGATGCTTGTCCCTATGGTGTAGT
ATACTTTAATAGCGAGAAAGCTCATGAATTTTGGAGAGATGGAAAGGCTCAAGAAGTTGTTAATAAAATAAGTAAAGAAA
ATGACGTTATTCCTCCTTACTATAATCCTAATAGAGCCAGAACTTATGCTGGGATACGCCCTCCAGATATTGTAGAAAAG
TGCACATTTTGTGATCATCTCATAATAGAAGGGAAATTACCTTATTGTGTAGAACAATGCCCTTCCGGTGCAAGGTATTT
TGGTGACAAAGAAGATGAGAATGATTTAGTAAATCAAATGCTAAAAGAATATATGAGCAAACGGTTAAAAGAATATAAAG
GAACTGAACCCAATGTATATTACATTAGAGATTACAATAAAAATGCTTAA

Upstream 100 bases:

>100_bases
GGTAGAAAAATCTTAAGATCGTCCAATTATTTGAATTAGTTATAAATAATTAATTTAGGGAGAAAATTGTCTCTCTGTTT
GACTTAGGAGGTGTTTACTG

Downstream 100 bases:

>100_bases
CAGTTATCCAAAAAAATAATAAAATAATGAAGTGAAAAAGAACTGTTTACAAAGGGGCGATAGTATATCATGACAATGCA
AGAAGAAAATTCTTTGGCAA

Product: 4Fe-4S ferredoxin iron-sulfur binding domain protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTKYAMVIDLHRCTGCGACAITCKIENNVPRGFRWADYIHETKGEFPNVTYTYIPTLCNHCDHAPCVEVCPTDPKAMYKT
EHGLTLHDSKECIGCRQCEDACPYGVVYFNSEKAHEFWRDGKAQEVVNKISKENDVIPPYYNPNRARTYAGIRPPDIVEK
CTFCDHLIIEGKLPYCVEQCPSGARYFGDKEDENDLVNQMLKEYMSKRLKEYKGTEPNVYYIRDYNKNA

Sequences:

>Translated_229_residues
MTKYAMVIDLHRCTGCGACAITCKIENNVPRGFRWADYIHETKGEFPNVTYTYIPTLCNHCDHAPCVEVCPTDPKAMYKT
EHGLTLHDSKECIGCRQCEDACPYGVVYFNSEKAHEFWRDGKAQEVVNKISKENDVIPPYYNPNRARTYAGIRPPDIVEK
CTFCDHLIIEGKLPYCVEQCPSGARYFGDKEDENDLVNQMLKEYMSKRLKEYKGTEPNVYYIRDYNKNA
>Mature_228_residues
TKYAMVIDLHRCTGCGACAITCKIENNVPRGFRWADYIHETKGEFPNVTYTYIPTLCNHCDHAPCVEVCPTDPKAMYKTE
HGLTLHDSKECIGCRQCEDACPYGVVYFNSEKAHEFWRDGKAQEVVNKISKENDVIPPYYNPNRARTYAGIRPPDIVEKC
TFCDHLIIEGKLPYCVEQCPSGARYFGDKEDENDLVNQMLKEYMSKRLKEYKGTEPNVYYIRDYNKNA

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=223, Percent_Identity=32.7354260089686, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787872, Length=199, Percent_Identity=32.6633165829146, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1787122, Length=195, Percent_Identity=31.7948717948718, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI226510944, Length=197, Percent_Identity=30.4568527918782, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787740, Length=149, Percent_Identity=31.5436241610738, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787478, Length=175, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082114, Length=185, Percent_Identity=25.9459459459459, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1789079, Length=198, Percent_Identity=25.2525252525253, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87082180, Length=112, Percent_Identity=34.8214285714286, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 26400; Mature: 26269

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
7.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYAMVIDLHRCTGCGACAITCKIENNVPRGFRWADYIHETKGEFPNVTYTYIPTLCNH
CCCEEEEEEEHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHCC
CDHAPCVEVCPTDPKAMYKTEHGLTLHDSKECIGCRQCEDACPYGVVYFNSEKAHEFWRD
CCCCCCEEECCCCCHHHEECCCCCEEECCHHHHCHHHHHHCCCEEEEEECCHHHHHHHHC
GKAQEVVNKISKENDVIPPYYNPNRARTYAGIRPPDIVEKCTFCDHLIIEGKLPYCVEQC
CCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHEEECCCCCHHHHHC
PSGARYFGDKEDENDLVNQMLKEYMSKRLKEYKGTEPNVYYIRDYNKNA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
TKYAMVIDLHRCTGCGACAITCKIENNVPRGFRWADYIHETKGEFPNVTYTYIPTLCNH
CCEEEEEEEHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHCC
CDHAPCVEVCPTDPKAMYKTEHGLTLHDSKECIGCRQCEDACPYGVVYFNSEKAHEFWRD
CCCCCCEEECCCCCHHHEECCCCCEEECCHHHHCHHHHHHCCCEEEEEECCHHHHHHHHC
GKAQEVVNKISKENDVIPPYYNPNRARTYAGIRPPDIVEKCTFCDHLIIEGKLPYCVEQC
CCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHEEECCCCCHHHHHC
PSGARYFGDKEDENDLVNQMLKEYMSKRLKEYKGTEPNVYYIRDYNKNA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]