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The map label for this gene is phsA [H]

Identifier: 188585281

GI number: 188585281

Start: 665176

End: 667776

Strand: Direct

Name: phsA [H]

Synonym: Nther_0643

Alternate gene names: 188585281

Gene position: 665176-667776 (Clockwise)

Preceding gene: 188585280

Following gene: 188585282

Centisome position: 21.01

GC content: 39.52

Gene sequence:

>2601_bases
TTGACTTTAAAAAAATTGCTCAATAAAGAAATAAAGCGAAGGACATTCCTAGCAGTTACCGGTGCAGCAGGGGCTGGTAT
AGCAATTGGACACGGATTGGCTAAACCGCCAGAAGAGGCAGAAGCCGAGGAAAAAGAAAAAGTAAAAGAAGTGTACGAGA
TGAGTAATCGGATCGGTGAAGTTGATGATTATGTAGAAGGCCAGTGGATACCTACAGGGTGTTCTGGTTGTACTTCTTGG
TGCAGTAAAGAAGCTAATGTGGTTAATGGACGTGTAATTAAAGTTAGAGGAAACAAGAACTCTAAAGTAAATGGTGAAGT
CAGTTGTCCCAGGGCCCATCTTTCATTACAACAACTTTACGATCCAGATAGGATAAAACAACCTATGAAACGGACAAATC
CTGACAAAGGAAGAGATGTTGATCCCGATTTTGTCCCCATCTCCTGGGATGAAGCTATGGAAGAAATAGCTGACAAGATT
TTGGAATTGAGAGAACAGGACGAGAACCATAAAATAGCTGTGCAAAGAGGAAGATATACCCAGTTGAGAGATATAATTTA
CAGCCATGTTCCTGCCATATTGGGGACTCCTAATAATATTTCACATAGTTCCATCTGTGCTGAAGCCGAAAAATTTGGTC
CCTACTATACTGAAGGATACTGGAATTATCGAGATTTTGACGTGCTCAACAGTGAGTATATTATTCTTTGGGGAGCAGAC
CCTATTGCTTCTAACAGGCAAATTTCTTATTATCTGGGAGTTTGGCCTGATGTTTTAGACAAAGCAAAAGTTGCTGTGAT
TGAGCCACGATTGTCAGCTAGTGCTACTAAAGCTCATGAGTGGTTACCGGTTAAACCGGCTGAAGACGGTGCTCTAGCCG
TGGCAATGGCCCATGTAATATTAACTCAAGGGCTTTGGAACAAAGAATTTGTTGGAGATTTTCACGATGGTGAAAATAGA
TTTATACCTGGAAAAACTGTAGATGAAGAAACTTTTGAAGAAAATTACACTCACGGGTTAGTTAAATGGTGGAATTTAGA
ATTGAAAGATAGGACACCAGAATGGGCTGAAGCTCGTTCAGGTGTGCCAAGGTCTCAAATCATACGGGTTGCAACTGAAT
TTGCAGAAAAAGCTCCCAAGGCATTGGTCTGGATGGGTGGAGGACCTGTTATGCAAGTACGAGGTGGATATACCTCCATG
GCAATTTCAGCACTAAATGGCCTTGTGGGCTCTGTTGATAGTGAAGGTGGCACTTTAAGAGGTGCAGGTGTACCTAGAAA
GAGTTATCCTGCACCTGATGAATATATGGATGAAAATGCTCAAGAAAAAATTAACTACCAAAAAATCGATCAAAGAGGAT
ACTTAGAATTCCCAGCTTTGAAAGAAGGAAAACCTGGTAGCGGAGTTGTTACAAACCGAATGGCTGATGGTATTTTAAAA
GAGGACCCTTATGATATTAAGATGATTATGGCTTATTGGAACAACTTTGCCTTTTCAGCACCTGAAGCCAGACGTTGGGA
AGAGGCCTTGAAAAAAGTAGATTTTATTGTTCATATGGTGACTCACGAATCTGAAATGACTAGGTATGCCGACATAGTTC
TACCGTCTACCCATCATATGTTTGAACAGTGGGGATTTTTAAACCAGAAAGGAAATAAACATACCCACTTATGGTTAGCT
AGACCAATGATAGAACGGTTTTTCGATGTAAAAGACCCTGAAGCTGAAGTGCAGTGGTTATTGGCTGAGAAACTAGCTGA
AAAAGGCTTTGATAAGCTTTTAGAACACTTTAAAAACTTTAAAGATCCTGAAACAGAAAAAGAACCAACTAATGAAATAG
AGTTTGCTGAATACGCAACTAAATTGACCTTACAGCCAATTTGGGATCCTAATGAGTATGAAAGTGGAGATCAGTTTGAT
GGCTGGGAAGACTTTAAAGCTGTTGGGGTATGGAATTCGGATGAATATAAATATAAACAGTACTGGGACAATTTTGGAAC
CAAGACTGATAAATATGAATTTTATAGTGAAACTTTAAAGGACGCTTTAGAAAAGCATGCAGCCAATCACAATCTTACAG
TCGATGAGGTTCTCGAAGCATGTAAATACGAAGCTGAAGGAGAAGTGGCTTTTATCCCTCATTATGACCCCCCCTTTGTG
CACGGTCAGAAAGAAGAATATCCCTTTATGTTTGTAGATTTTAAAAATAGGCTGAACAGAGAAGGACGTTCAGCAAATTG
TTATTGGTATCATGAATTTAATGATATCAATCCAGGTTTAGAGAAGGAAAAAGATGTGGCTTTATTAAATCCAAAGGATG
CCGATGAATATGGCATAAAAACTGATGATAAGATTATTATAAAATCACCTGTAGGAGAAATAGAATGTTATGCAAAATTA
TGGGAAGGAATACCTCCAGGAGTTGTTGCTAAAGCTTACGGCCAAGGACATTGGGCTTATGGACGAGTAGCCAGTGAAAA
TTTTGACAGAGGAATTCCCCGTGGGGGGAATAATAATGAAATTTTAGTTGCGGATTATGAAAGGCTTAGCGGAAGTACGG
CTTTTTATTCCCATACCAGAGTGAGGGTAGAAAAATCTTAA

Upstream 100 bases:

>100_bases
GGTGATAAATGTATTTAACTAGAAGAAAACTCATAAAATTTAGTGAATATTCACAAAACAATGGTTTTAACAACCTTTTC
TCGAGACGGAGGTGAATTAT

Downstream 100 bases:

>100_bases
GATCGTCCAATTATTTGAATTAGTTATAAATAATTAATTTAGGGAGAAAATTGTCTCTCTGTTTGACTTAGGAGGTGTTT
ACTGTTGACTAAATATGCTA

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 866; Mature: 865

Protein sequence:

>866_residues
MTLKKLLNKEIKRRTFLAVTGAAGAGIAIGHGLAKPPEEAEAEEKEKVKEVYEMSNRIGEVDDYVEGQWIPTGCSGCTSW
CSKEANVVNGRVIKVRGNKNSKVNGEVSCPRAHLSLQQLYDPDRIKQPMKRTNPDKGRDVDPDFVPISWDEAMEEIADKI
LELREQDENHKIAVQRGRYTQLRDIIYSHVPAILGTPNNISHSSICAEAEKFGPYYTEGYWNYRDFDVLNSEYIILWGAD
PIASNRQISYYLGVWPDVLDKAKVAVIEPRLSASATKAHEWLPVKPAEDGALAVAMAHVILTQGLWNKEFVGDFHDGENR
FIPGKTVDEETFEENYTHGLVKWWNLELKDRTPEWAEARSGVPRSQIIRVATEFAEKAPKALVWMGGGPVMQVRGGYTSM
AISALNGLVGSVDSEGGTLRGAGVPRKSYPAPDEYMDENAQEKINYQKIDQRGYLEFPALKEGKPGSGVVTNRMADGILK
EDPYDIKMIMAYWNNFAFSAPEARRWEEALKKVDFIVHMVTHESEMTRYADIVLPSTHHMFEQWGFLNQKGNKHTHLWLA
RPMIERFFDVKDPEAEVQWLLAEKLAEKGFDKLLEHFKNFKDPETEKEPTNEIEFAEYATKLTLQPIWDPNEYESGDQFD
GWEDFKAVGVWNSDEYKYKQYWDNFGTKTDKYEFYSETLKDALEKHAANHNLTVDEVLEACKYEAEGEVAFIPHYDPPFV
HGQKEEYPFMFVDFKNRLNREGRSANCYWYHEFNDINPGLEKEKDVALLNPKDADEYGIKTDDKIIIKSPVGEIECYAKL
WEGIPPGVVAKAYGQGHWAYGRVASENFDRGIPRGGNNNEILVADYERLSGSTAFYSHTRVRVEKS

Sequences:

>Translated_866_residues
MTLKKLLNKEIKRRTFLAVTGAAGAGIAIGHGLAKPPEEAEAEEKEKVKEVYEMSNRIGEVDDYVEGQWIPTGCSGCTSW
CSKEANVVNGRVIKVRGNKNSKVNGEVSCPRAHLSLQQLYDPDRIKQPMKRTNPDKGRDVDPDFVPISWDEAMEEIADKI
LELREQDENHKIAVQRGRYTQLRDIIYSHVPAILGTPNNISHSSICAEAEKFGPYYTEGYWNYRDFDVLNSEYIILWGAD
PIASNRQISYYLGVWPDVLDKAKVAVIEPRLSASATKAHEWLPVKPAEDGALAVAMAHVILTQGLWNKEFVGDFHDGENR
FIPGKTVDEETFEENYTHGLVKWWNLELKDRTPEWAEARSGVPRSQIIRVATEFAEKAPKALVWMGGGPVMQVRGGYTSM
AISALNGLVGSVDSEGGTLRGAGVPRKSYPAPDEYMDENAQEKINYQKIDQRGYLEFPALKEGKPGSGVVTNRMADGILK
EDPYDIKMIMAYWNNFAFSAPEARRWEEALKKVDFIVHMVTHESEMTRYADIVLPSTHHMFEQWGFLNQKGNKHTHLWLA
RPMIERFFDVKDPEAEVQWLLAEKLAEKGFDKLLEHFKNFKDPETEKEPTNEIEFAEYATKLTLQPIWDPNEYESGDQFD
GWEDFKAVGVWNSDEYKYKQYWDNFGTKTDKYEFYSETLKDALEKHAANHNLTVDEVLEACKYEAEGEVAFIPHYDPPFV
HGQKEEYPFMFVDFKNRLNREGRSANCYWYHEFNDINPGLEKEKDVALLNPKDADEYGIKTDDKIIIKSPVGEIECYAKL
WEGIPPGVVAKAYGQGHWAYGRVASENFDRGIPRGGNNNEILVADYERLSGSTAFYSHTRVRVEKS
>Mature_865_residues
TLKKLLNKEIKRRTFLAVTGAAGAGIAIGHGLAKPPEEAEAEEKEKVKEVYEMSNRIGEVDDYVEGQWIPTGCSGCTSWC
SKEANVVNGRVIKVRGNKNSKVNGEVSCPRAHLSLQQLYDPDRIKQPMKRTNPDKGRDVDPDFVPISWDEAMEEIADKIL
ELREQDENHKIAVQRGRYTQLRDIIYSHVPAILGTPNNISHSSICAEAEKFGPYYTEGYWNYRDFDVLNSEYIILWGADP
IASNRQISYYLGVWPDVLDKAKVAVIEPRLSASATKAHEWLPVKPAEDGALAVAMAHVILTQGLWNKEFVGDFHDGENRF
IPGKTVDEETFEENYTHGLVKWWNLELKDRTPEWAEARSGVPRSQIIRVATEFAEKAPKALVWMGGGPVMQVRGGYTSMA
ISALNGLVGSVDSEGGTLRGAGVPRKSYPAPDEYMDENAQEKINYQKIDQRGYLEFPALKEGKPGSGVVTNRMADGILKE
DPYDIKMIMAYWNNFAFSAPEARRWEEALKKVDFIVHMVTHESEMTRYADIVLPSTHHMFEQWGFLNQKGNKHTHLWLAR
PMIERFFDVKDPEAEVQWLLAEKLAEKGFDKLLEHFKNFKDPETEKEPTNEIEFAEYATKLTLQPIWDPNEYESGDQFDG
WEDFKAVGVWNSDEYKYKQYWDNFGTKTDKYEFYSETLKDALEKHAANHNLTVDEVLEACKYEAEGEVAFIPHYDPPFVH
GQKEEYPFMFVDFKNRLNREGRSANCYWYHEFNDINPGLEKEKDVALLNPKDADEYGIKTDDKIIIKSPVGEIECYAKLW
EGIPPGVVAKAYGQGHWAYGRVASENFDRGIPRGGNNNEILVADYERLSGSTAFYSHTRVRVEKS

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=794, Percent_Identity=25.4408060453401, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1787870, Length=860, Percent_Identity=22.906976744186, Blast_Score=141, Evalue=2e-34,
Organism=Escherichia coli, GI171474008, Length=872, Percent_Identity=22.9357798165138, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI3868721, Length=358, Percent_Identity=24.0223463687151, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 98735; Mature: 98603

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKKLLNKEIKRRTFLAVTGAAGAGIAIGHGLAKPPEEAEAEEKEKVKEVYEMSNRIGE
CCHHHHHHHHHHHEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VDDYVEGQWIPTGCSGCTSWCSKEANVVNGRVIKVRGNKNSKVNGEVSCPRAHLSLQQLY
CHHHHCCCEECCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHC
DPDRIKQPMKRTNPDKGRDVDPDFVPISWDEAMEEIADKILELREQDENHKIAVQRGRYT
CCHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHH
QLRDIIYSHVPAILGTPNNISHSSICAEAEKFGPYYTEGYWNYRDFDVLNSEYIILWGAD
HHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCEEECCCCEEEEECCC
PIASNRQISYYLGVWPDVLDKAKVAVIEPRLSASATKAHEWLPVKPAEDGALAVAMAHVI
CCCCCCEEEEEECCCHHHHCCCCEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHH
LTQGLWNKEFVGDFHDGENRFIPGKTVDEETFEENYTHGLVKWWNLELKDRTPEWAEARS
HHCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHC
GVPRSQIIRVATEFAEKAPKALVWMGGGPVMQVRGGYTSMAISALNGLVGSVDSEGGTLR
CCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCEEE
GAGVPRKSYPAPDEYMDENAQEKINYQKIDQRGYLEFPALKEGKPGSGVVTNRMADGILK
CCCCCCCCCCCCHHHHCCCHHHHCCHHHHCCCCCEECCCCCCCCCCCCEEHHHHHCCCCC
EDPYDIKMIMAYWNNFAFSAPEARRWEEALKKVDFIVHMVTHESEMTRYADIVLPSTHHM
CCCCCCEEHEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEECCHHHH
FEQWGFLNQKGNKHTHLWLARPMIERFFDVKDPEAEVQWLLAEKLAEKGFDKLLEHFKNF
HHHCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
KDPETEKEPTNEIEFAEYATKLTLQPIWDPNEYESGDQFDGWEDFKAVGVWNSDEYKYKQ
CCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHCEEEECCCCCCHHHHH
YWDNFGTKTDKYEFYSETLKDALEKHAANHNLTVDEVLEACKYEAEGEVAFIPHYDPPFV
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCC
HGQKEEYPFMFVDFKNRLNREGRSANCYWYHEFNDINPGLEKEKDVALLNPKDADEYGIK
CCCCCCCCEEEEECHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCHHCCCC
TDDKIIIKSPVGEIECYAKLWEGIPPGVVAKAYGQGHWAYGRVASENFDRGIPRGGNNNE
CCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCCEEECCHHHCCCCCCCCCCCCCCE
ILVADYERLSGSTAFYSHTRVRVEKS
EEEEEHHHCCCCEEEEEEEEEEEECC
>Mature Secondary Structure 
TLKKLLNKEIKRRTFLAVTGAAGAGIAIGHGLAKPPEEAEAEEKEKVKEVYEMSNRIGE
CHHHHHHHHHHHEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VDDYVEGQWIPTGCSGCTSWCSKEANVVNGRVIKVRGNKNSKVNGEVSCPRAHLSLQQLY
CHHHHCCCEECCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHC
DPDRIKQPMKRTNPDKGRDVDPDFVPISWDEAMEEIADKILELREQDENHKIAVQRGRYT
CCHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHH
QLRDIIYSHVPAILGTPNNISHSSICAEAEKFGPYYTEGYWNYRDFDVLNSEYIILWGAD
HHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCEEECCCCEEEEECCC
PIASNRQISYYLGVWPDVLDKAKVAVIEPRLSASATKAHEWLPVKPAEDGALAVAMAHVI
CCCCCCEEEEEECCCHHHHCCCCEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHH
LTQGLWNKEFVGDFHDGENRFIPGKTVDEETFEENYTHGLVKWWNLELKDRTPEWAEARS
HHCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHC
GVPRSQIIRVATEFAEKAPKALVWMGGGPVMQVRGGYTSMAISALNGLVGSVDSEGGTLR
CCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCEEE
GAGVPRKSYPAPDEYMDENAQEKINYQKIDQRGYLEFPALKEGKPGSGVVTNRMADGILK
CCCCCCCCCCCCHHHHCCCHHHHCCHHHHCCCCCEECCCCCCCCCCCCEEHHHHHCCCCC
EDPYDIKMIMAYWNNFAFSAPEARRWEEALKKVDFIVHMVTHESEMTRYADIVLPSTHHM
CCCCCCEEHEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEECCHHHH
FEQWGFLNQKGNKHTHLWLARPMIERFFDVKDPEAEVQWLLAEKLAEKGFDKLLEHFKNF
HHHCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
KDPETEKEPTNEIEFAEYATKLTLQPIWDPNEYESGDQFDGWEDFKAVGVWNSDEYKYKQ
CCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHCEEEECCCCCCHHHHH
YWDNFGTKTDKYEFYSETLKDALEKHAANHNLTVDEVLEACKYEAEGEVAFIPHYDPPFV
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCC
HGQKEEYPFMFVDFKNRLNREGRSANCYWYHEFNDINPGLEKEKDVALLNPKDADEYGIK
CCCCCCCCEEEEECHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCHHCCCC
TDDKIIIKSPVGEIECYAKLWEGIPPGVVAKAYGQGHWAYGRVASENFDRGIPRGGNNNE
CCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCCEEECCHHHCCCCCCCCCCCCCCE
ILVADYERLSGSTAFYSHTRVRVEKS
EEEEEHHHCCCCEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]