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The map label for this gene is odhL [H]

Identifier: 188585220

GI number: 188585220

Start: 608803

End: 610314

Strand: Direct

Name: odhL [H]

Synonym: Nther_0582

Alternate gene names: 188585220

Gene position: 608803-610314 (Clockwise)

Preceding gene: 188585218

Following gene: 188585221

Centisome position: 19.23

GC content: 35.58

Gene sequence:

>1512_bases
ATGCTATTCTTTCAAGGAGGGAAAAATATCAAAAAGCAAAATAAATTTTCAAATCAATATGACCTGGTAGTAATAGGTGG
AGGACCAGGTGGGACTGCTTGTGCTTTAAAAGCTGCAAGATTAGGATTAACAACTGCTTTGATAGAAAAAGATTTCCCTG
GAGGAGGATCTGTTGCCAGGGGGTATTTTCCTGTAAAAGCCCTAATAGAAGCGGCTAAATTAAAAGAAAATAAATTAGCT
CCAAATGAAGAGTGCCTGGCCAAAGCTAATGAGAGGGTAAATAAAGCTAGAGCTCAGTGGGATTCTAGCCTGACTAAAAG
GTTTGAACAATTATATCTAATTCCCGGTGAAGGAGAGTTACTCTCAAGTACATCTGAATTTTATCAAATTAAAATTCACT
CAAGTAAATATCAAGGGGAAATCCAGGACCCAAATATCATAGATGGTGATCGGTATATTAGTGCGAAATCCATAGTAATG
GCTACGGGCACGGCTCCAGGGTCGCCTATACCTGAACTAACTATTGATGGGAAGAATGTTATTACTCATGAGCACTTATT
AGATATGCATTATAATAACATTAACTCTATAGCTATTATAGGTGCTGATGTAGAAGGCTGTGAGTTTGCCGGTCTATTTA
ACCGACTTGGGATACAAGTACATCTGTTAGAAATGGAAGATAATATATTGCCAAATTGTGATCAGGAAATTTCTAATAGT
TTATCGACTGAATATAAGAAACAAGGAATAAAAATAAAAACAAATACTCAAGTTACTGGAGTTCAAAACTTAGAAGGTGA
AGCCTTAAAGCTTACTTATAATTCGAATACTCCACAAGCCCATGGTGATTATTATTCTTCCAAAACAGAAAATGAATCAG
ATAAATCCCTGGAAGTTGATAAAATATTGGTTACTGGCAAGAGAATACCTCAATTACCTAAGGGCTACGCTAACCTTCCC
TTGAAAATTACCCAGGAAGGTTTGGTAGAGGTTAATTCTCAATTGGAAAGTTCAGCACCTGAAGTTTATGTCATTGGTGA
TCTTGCAGGTGGTGTTCCCTCAGCCAATGCTGCTATTCATGAAGGAAAGCTGGTCTCCCAAAATTTAACAGAACCAAGAA
AAAATAAACATATTAATTATTCCTTTACTCCTTATGTTTTTTTTACAGATCCCCAAACTTCAGGAATAGGAGTTACCGAA
GACTATTTAAGAAATAAAAAAATTCCCTATAAAAAAGGGATAGCCTATTTTAAAGAAAACTTGAGGACCTTATCATTAGG
ACATGATAATGGCTTTGTCAAAGTTTTGCTAGGTAAAAATGAATCCCTCTTAGGAGTTCACATGATAGGGCATGATATTT
CAGAGTTGATATCAGTTATAACAGTTGCGATGCAATCAAAAATTCCTGCAAGTAAAATAGTTGATTTACCTCTTCCTCAT
CCAAGTATGGGTGAACTTATGATAGAAGCAATTGAAGAAGCAATAAATACAAGAATAACAACTACTGGTTAA

Upstream 100 bases:

>100_bases
GAACTTTATGTGCAACTTGCCACATTTTTATCTCTCTCCTTAGTTAATAGTTTTGAAGATGTATGTATCCAAGCAGGCAT
AGTATTTCTATTTTAGATTA

Downstream 100 bases:

>100_bases
CAACAAACAATATTGATTATAATTGCTATAAATATTTTCTTGAAATAATAACAATTATTAGTTATACTGGAAAAAACAGA
AACCTTAACAAGGAGGAGAA

Product: pyridine nucleotide-disulphide oxidoreductase dimerisation region

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MLFFQGGKNIKKQNKFSNQYDLVVIGGGPGGTACALKAARLGLTTALIEKDFPGGGSVARGYFPVKALIEAAKLKENKLA
PNEECLAKANERVNKARAQWDSSLTKRFEQLYLIPGEGELLSSTSEFYQIKIHSSKYQGEIQDPNIIDGDRYISAKSIVM
ATGTAPGSPIPELTIDGKNVITHEHLLDMHYNNINSIAIIGADVEGCEFAGLFNRLGIQVHLLEMEDNILPNCDQEISNS
LSTEYKKQGIKIKTNTQVTGVQNLEGEALKLTYNSNTPQAHGDYYSSKTENESDKSLEVDKILVTGKRIPQLPKGYANLP
LKITQEGLVEVNSQLESSAPEVYVIGDLAGGVPSANAAIHEGKLVSQNLTEPRKNKHINYSFTPYVFFTDPQTSGIGVTE
DYLRNKKIPYKKGIAYFKENLRTLSLGHDNGFVKVLLGKNESLLGVHMIGHDISELISVITVAMQSKIPASKIVDLPLPH
PSMGELMIEAIEEAINTRITTTG

Sequences:

>Translated_503_residues
MLFFQGGKNIKKQNKFSNQYDLVVIGGGPGGTACALKAARLGLTTALIEKDFPGGGSVARGYFPVKALIEAAKLKENKLA
PNEECLAKANERVNKARAQWDSSLTKRFEQLYLIPGEGELLSSTSEFYQIKIHSSKYQGEIQDPNIIDGDRYISAKSIVM
ATGTAPGSPIPELTIDGKNVITHEHLLDMHYNNINSIAIIGADVEGCEFAGLFNRLGIQVHLLEMEDNILPNCDQEISNS
LSTEYKKQGIKIKTNTQVTGVQNLEGEALKLTYNSNTPQAHGDYYSSKTENESDKSLEVDKILVTGKRIPQLPKGYANLP
LKITQEGLVEVNSQLESSAPEVYVIGDLAGGVPSANAAIHEGKLVSQNLTEPRKNKHINYSFTPYVFFTDPQTSGIGVTE
DYLRNKKIPYKKGIAYFKENLRTLSLGHDNGFVKVLLGKNESLLGVHMIGHDISELISVITVAMQSKIPASKIVDLPLPH
PSMGELMIEAIEEAINTRITTTG
>Mature_503_residues
MLFFQGGKNIKKQNKFSNQYDLVVIGGGPGGTACALKAARLGLTTALIEKDFPGGGSVARGYFPVKALIEAAKLKENKLA
PNEECLAKANERVNKARAQWDSSLTKRFEQLYLIPGEGELLSSTSEFYQIKIHSSKYQGEIQDPNIIDGDRYISAKSIVM
ATGTAPGSPIPELTIDGKNVITHEHLLDMHYNNINSIAIIGADVEGCEFAGLFNRLGIQVHLLEMEDNILPNCDQEISNS
LSTEYKKQGIKIKTNTQVTGVQNLEGEALKLTYNSNTPQAHGDYYSSKTENESDKSLEVDKILVTGKRIPQLPKGYANLP
LKITQEGLVEVNSQLESSAPEVYVIGDLAGGVPSANAAIHEGKLVSQNLTEPRKNKHINYSFTPYVFFTDPQTSGIGVTE
DYLRNKKIPYKKGIAYFKENLRTLSLGHDNGFVKVLLGKNESLLGVHMIGHDISELISVITVAMQSKIPASKIVDLPLPH
PSMGELMIEAIEEAINTRITTTG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=482, Percent_Identity=27.5933609958506, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI50301238, Length=488, Percent_Identity=25, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI291045266, Length=517, Percent_Identity=24.5647969052224, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI87082354, Length=484, Percent_Identity=26.4462809917355, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1786307, Length=477, Percent_Identity=22.8511530398323, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87081717, Length=478, Percent_Identity=23.6401673640167, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1789915, Length=470, Percent_Identity=23.8297872340426, Blast_Score=86, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI32565766, Length=482, Percent_Identity=29.253112033195, Blast_Score=176, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6325240, Length=512, Percent_Identity=27.9296875, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6321091, Length=487, Percent_Identity=28.1314168377823, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6325166, Length=511, Percent_Identity=25.0489236790607, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21358499, Length=487, Percent_Identity=26.8993839835729, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640549, Length=509, Percent_Identity=22.5933202357564, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24640553, Length=507, Percent_Identity=22.4852071005917, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24640551, Length=509, Percent_Identity=22.5933202357564, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 54987; Mature: 54987

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFFQGGKNIKKQNKFSNQYDLVVIGGGPGGTACALKAARLGLTTALIEKDFPGGGSVAR
CEEECCCCCCCHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GYFPVKALIEAAKLKENKLAPNEECLAKANERVNKARAQWDSSLTKRFEQLYLIPGEGEL
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCH
LSSTSEFYQIKIHSSKYQGEIQDPNIIDGDRYISAKSIVMATGTAPGSPIPELTIDGKNV
HCCCCEEEEEEEECCCCCCCCCCCCEECCCCEEEECEEEEEECCCCCCCCCEEEECCCCE
ITHEHLLDMHYNNINSIAIIGADVEGCEFAGLFNRLGIQVHLLEMEDNILPNCDQEISNS
EEHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHH
LSTEYKKQGIKIKTNTQVTGVQNLEGEALKLTYNSNTPQAHGDYYSSKTENESDKSLEVD
HHHHHHHCCEEEEECCEEECEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEE
KILVTGKRIPQLPKGYANLPLKITQEGLVEVNSQLESSAPEVYVIGDLAGGVPSANAAIH
EEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEE
EGKLVSQNLTEPRKNKHINYSFTPYVFFTDPQTSGIGVTEDYLRNKKIPYKKGIAYFKEN
CCEEHHCCCCCCHHCCEEEEEECEEEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHCC
LRTLSLGHDNGFVKVLLGKNESLLGVHMIGHDISELISVITVAMQSKIPASKIVDLPLPH
CEEEEECCCCCEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCC
PSMGELMIEAIEEAINTRITTTG
CCHHHHHHHHHHHHHCCEEEECC
>Mature Secondary Structure
MLFFQGGKNIKKQNKFSNQYDLVVIGGGPGGTACALKAARLGLTTALIEKDFPGGGSVAR
CEEECCCCCCCHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GYFPVKALIEAAKLKENKLAPNEECLAKANERVNKARAQWDSSLTKRFEQLYLIPGEGEL
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCH
LSSTSEFYQIKIHSSKYQGEIQDPNIIDGDRYISAKSIVMATGTAPGSPIPELTIDGKNV
HCCCCEEEEEEEECCCCCCCCCCCCEECCCCEEEECEEEEEECCCCCCCCCEEEECCCCE
ITHEHLLDMHYNNINSIAIIGADVEGCEFAGLFNRLGIQVHLLEMEDNILPNCDQEISNS
EEHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHH
LSTEYKKQGIKIKTNTQVTGVQNLEGEALKLTYNSNTPQAHGDYYSSKTENESDKSLEVD
HHHHHHHCCEEEEECCEEECEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEE
KILVTGKRIPQLPKGYANLPLKITQEGLVEVNSQLESSAPEVYVIGDLAGGVPSANAAIH
EEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEE
EGKLVSQNLTEPRKNKHINYSFTPYVFFTDPQTSGIGVTEDYLRNKKIPYKKGIAYFKEN
CCEEHHCCCCCCHHCCEEEEEECEEEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHCC
LRTLSLGHDNGFVKVLLGKNESLLGVHMIGHDISELISVITVAMQSKIPASKIVDLPLPH
CEEEEECCCCCEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCC
PSMGELMIEAIEEAINTRITTTG
CCHHHHHHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]