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The map label for this gene is yibK [C]

Identifier: 188585180

GI number: 188585180

Start: 564060

End: 564518

Strand: Direct

Name: yibK [C]

Synonym: Nther_0541

Alternate gene names: 188585180

Gene position: 564060-564518 (Clockwise)

Preceding gene: 188585179

Following gene: 188585181

Centisome position: 17.82

GC content: 34.86

Gene sequence:

>459_bases
ATGCATGTAGTACTAGTGGAACCTGAAATCCCACCTAATACAGGTAATATTTCCAGGACTTGTGCAGTCAGTAATACAAC
TTTACATTTAGTTGAACCTTTGGGTTTTTCAATTTCCGACCGGGAGTTAAAAAGGGCAGGTCTCGATTACTGGGACAAAT
TATCATATCAAGTTCATGAAAATTTCACATCTGTTGAAAACTTATTACAAACACATCCCATTTATATTGCAACAACTCGA
GGTAAAAATTATTATTATGATATAGAATATCCCCGTGATAGTGTTTTGGTATTTGGAAAAGAGACTGAAGGTTTGCCGGA
TTGGATTATTAATAAGTATTATGAAAACTCCATTCGAATACCAATGGCTCCAGATTTTAGATCCCTTAATTTGAGTAACT
CTGTTGCTGTTGTATTGTTTGAAGCTTTGCGACAGCAAAGTTTCCCAGGATTAAATTAA

Upstream 100 bases:

>100_bases
CTGCCGTGTTTTATAAGGATATTGTTATATTTTAATATGAATATTATAATAGGTATTGAGTAATATAAATACTTTCTTTT
TTAATGGAGGCGAGCTGGCA

Downstream 100 bases:

>100_bases
AATTGTCAACGAAAAGGAGAATTGTATGGGAAAATTTACAGCTATTTTGTTTGACTTAGATGGAACTTTGTTGCCTGTAG
ATACTAAGGTGTTTATTTAC

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MHVVLVEPEIPPNTGNISRTCAVSNTTLHLVEPLGFSISDRELKRAGLDYWDKLSYQVHENFTSVENLLQTHPIYIATTR
GKNYYYDIEYPRDSVLVFGKETEGLPDWIINKYYENSIRIPMAPDFRSLNLSNSVAVVLFEALRQQSFPGLN

Sequences:

>Translated_152_residues
MHVVLVEPEIPPNTGNISRTCAVSNTTLHLVEPLGFSISDRELKRAGLDYWDKLSYQVHENFTSVENLLQTHPIYIATTR
GKNYYYDIEYPRDSVLVFGKETEGLPDWIINKYYENSIRIPMAPDFRSLNLSNSVAVVLFEALRQQSFPGLN
>Mature_152_residues
MHVVLVEPEIPPNTGNISRTCAVSNTTLHLVEPLGFSISDRELKRAGLDYWDKLSYQVHENFTSVENLLQTHPIYIATTR
GKNYYYDIEYPRDSVLVFGKETEGLPDWIINKYYENSIRIPMAPDFRSLNLSNSVAVVLFEALRQQSFPGLN

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=151, Percent_Identity=44.3708609271523, Blast_Score=136, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17424; Mature: 17424

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVVLVEPEIPPNTGNISRTCAVSNTTLHLVEPLGFSISDRELKRAGLDYWDKLSYQVHE
CEEEEECCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHC
NFTSVENLLQTHPIYIATTRGKNYYYDIEYPRDSVLVFGKETEGLPDWIINKYYENSIRI
CHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCEEE
PMAPDFRSLNLSNSVAVVLFEALRQQSFPGLN
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHVVLVEPEIPPNTGNISRTCAVSNTTLHLVEPLGFSISDRELKRAGLDYWDKLSYQVHE
CEEEEECCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHC
NFTSVENLLQTHPIYIATTRGKNYYYDIEYPRDSVLVFGKETEGLPDWIINKYYENSIRI
CHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCEEE
PMAPDFRSLNLSNSVAVVLFEALRQQSFPGLN
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA