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The map label for this gene is hyfB [H]

Identifier: 188585148

GI number: 188585148

Start: 532282

End: 534528

Strand: Direct

Name: hyfB [H]

Synonym: Nther_0509

Alternate gene names: 188585148

Gene position: 532282-534528 (Clockwise)

Preceding gene: 188585147

Following gene: 188585149

Centisome position: 16.81

GC content: 35.83

Gene sequence:

>2247_bases
ATGACGGATCTGGTAACAGAACAAGCAGAATTTACGTCAATATTGCCTATATTAATTATATTTGTCCCCTTATTGGGATC
TTTTGGCGTTATTTTAATAAGTTCTGTTTCAGAAAAGCTTAGAGATATCTATACAGTCATTATTTCTGCTGTCCCTCTTG
TACTGTTAGCAGTGATGTATCCATTGGTACAACAGGGAATAGTTCAGTATAATATAGAGTGGTTTTTAGAAATTGGTTTG
ATTTTTAGATTGGACTCACTTGGTTTCTTATTTACGGCTCTAATTTCATTGATTTGGTTTTTAGCGACTTTATATGCAAC
AGAATATATCAGTCATGAACATAATCGCAATCGGTTTTACTTTTTTTGGTTGTTCACCTTTGGTGCTACTTTAGGTGTAT
TTGTAACAGGAGACTTGTTTGGTCTGTTTGTATTTTTTGAAATGATGAGTTTGACCAGTTATGTCCTTGTAATTCATGAA
GAAGATAAAGATGCTATGAGAGCAGGTAATCTATTCCTATTTTTAGGGATAGGTGGCGGTTTGGCTATATTATTTGGATT
ATTTATTATGTACTTTAACCTGGAAACTCTGGAAATCGCTCCTATGTTAGGAGATATGGTGGCAGCTGGAGTTAATATTC
CTGTTCTGTTATTATTATTTATTATCGGTTTTGGAATTAAAGCCGGGATTGTACCATTGCATATATGGTTGCCTAAAGCT
CATCCAGTAGCTCCTACACCTGCTAGTGCAATCTTGTCGGGCTTATTGATAAAAACAGGTGTATATGGTTTGTGGCGGGT
ATTTTTGATGGTTATGGGACCGGCTTCAAGCCAAGAGGTGGAACTGTACCAGCAATATATTACAAATTGGGGTATGGGAC
TATTTTGGATCGGTATTATTACAATGGTTTTGGGAGCCTTAATGGCTTTATTACAAACCAGTGCTAAAAAAATGTTAGCT
TACAGTAGCGTGAGTCAAGTCGGTTTTATTATTATGGGTTTAGGTGCTGCTGTATACTTGGGTACAGATGGTCCCATAGG
ATTTGCTGGTGGAATTATGCATGTAATAAATCACGCGCTCTTCAAAGCTTGTTTATTTTTAATAGTCGGTGCTATATTTA
TCAGGACTCATCTCTTAGATATAGACAGGGTAAGAGGTATGTTCAAACAAATGCCATTTATGGGAATTGCTTTTATCATT
GCTGCACTAGGAATAGCTGGTGTGCCTCTTTTCAATGGTTATGCTAGTAAAACCATATTGCATCATGCATTATCTGAAGT
TTCGTATTTAACACCAGGGACGGCTATGTTTATTGCTGATAAAATTTTTGTATTTACAAGTGCCTTAACAGTATCCTATT
TTATTAAATTGTTTAGGGGGTTATTTTTGGGGAAACTACCTCAAGAATGGCAAAACACCAATTTTTCTGTAGCCCCTATA
ATTAAAGTTGTGGTATCAGTATTCGTAGTGTTAATAATCGCTATCGGTGTTTTCCCGAATTTTGTTTTGGAACAGTTCGT
TATGCCTACTATGGCAGGCTTTCCTTACGAAAGTGAAGTTGTGACTTCACAATTACAAGGTATGGATTTGTTTACAAGTA
AAGATCTGTCCAAGGTAGTTGTTGTATTTTTAATTGCTGGCACAATAACTCTTGCTGATCAAAAATTCCACTGGTCTAGA
ATTACTCCGCCCTATTGGTTAAGTATAGAAGCCTTAGTTTATCGAAATGCCATTACAAAACTTATGTCAGGGATTGAAAA
ATTAGGAACCACCTTTGATGTTTCAGTGCAAGATTTTTATGAAACTACTGGAAGGACCTCTGTTAAGGTCTGTCGAAAAT
TAAGTGAACTGGACAAAACTCTAACTGATGCGTATGATAAGAGTGGTGAAGGCGCTATGAAGTTATCAGAAAAGACTGGT
CAATTAGACCGTCAGCTTGATAGATTCTATGATTCAACAGGTGAAAAAGCAAGCTCTATGACAGTTCTTTGGCCTGTTTC
AAAAGATGGAGATAAAACTTTAGCCCAAAAAGAGTTTGAAGCAGAACTTGAAAAGGCTTCTAGTAAACAGGGAAAGGTAA
GTAAGTATGTTACCCGGGATAGAAAAGCCAGGACTGGTTTCTTGGGTAAGATTAATTTTGATCCATCAAAATTAACGGTT
AAAAATCTAAACTTTGATTCTTTTGTGATAGCTTTTATGCTAGGAATTTCTTTACTGATCTTAATTTTTTACCATAGAAT
ATTTTAG

Upstream 100 bases:

>100_bases
ATTATTTTTATCGGATTATATCCGGCACTTCCCTTGAGCTTGGTAGAACCTGCTGTAGAATTTTTAATGCCGGAATTGTA
ATTTAAAGGGGGCGTTGTCC

Downstream 100 bases:

>100_bases
TTGAAAATATTGGCAAAGATAACCCCGTAATTTTTTATCAATTTAAACGTTTTTAATGTTATGATATCTCTAAGGAGGTT
ACTATATGAATACAAACAAA

Product: NADH dehydrogenase (quinone)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 748; Mature: 747

Protein sequence:

>748_residues
MTDLVTEQAEFTSILPILIIFVPLLGSFGVILISSVSEKLRDIYTVIISAVPLVLLAVMYPLVQQGIVQYNIEWFLEIGL
IFRLDSLGFLFTALISLIWFLATLYATEYISHEHNRNRFYFFWLFTFGATLGVFVTGDLFGLFVFFEMMSLTSYVLVIHE
EDKDAMRAGNLFLFLGIGGGLAILFGLFIMYFNLETLEIAPMLGDMVAAGVNIPVLLLLFIIGFGIKAGIVPLHIWLPKA
HPVAPTPASAILSGLLIKTGVYGLWRVFLMVMGPASSQEVELYQQYITNWGMGLFWIGIITMVLGALMALLQTSAKKMLA
YSSVSQVGFIIMGLGAAVYLGTDGPIGFAGGIMHVINHALFKACLFLIVGAIFIRTHLLDIDRVRGMFKQMPFMGIAFII
AALGIAGVPLFNGYASKTILHHALSEVSYLTPGTAMFIADKIFVFTSALTVSYFIKLFRGLFLGKLPQEWQNTNFSVAPI
IKVVVSVFVVLIIAIGVFPNFVLEQFVMPTMAGFPYESEVVTSQLQGMDLFTSKDLSKVVVVFLIAGTITLADQKFHWSR
ITPPYWLSIEALVYRNAITKLMSGIEKLGTTFDVSVQDFYETTGRTSVKVCRKLSELDKTLTDAYDKSGEGAMKLSEKTG
QLDRQLDRFYDSTGEKASSMTVLWPVSKDGDKTLAQKEFEAELEKASSKQGKVSKYVTRDRKARTGFLGKINFDPSKLTV
KNLNFDSFVIAFMLGISLLILIFYHRIF

Sequences:

>Translated_748_residues
MTDLVTEQAEFTSILPILIIFVPLLGSFGVILISSVSEKLRDIYTVIISAVPLVLLAVMYPLVQQGIVQYNIEWFLEIGL
IFRLDSLGFLFTALISLIWFLATLYATEYISHEHNRNRFYFFWLFTFGATLGVFVTGDLFGLFVFFEMMSLTSYVLVIHE
EDKDAMRAGNLFLFLGIGGGLAILFGLFIMYFNLETLEIAPMLGDMVAAGVNIPVLLLLFIIGFGIKAGIVPLHIWLPKA
HPVAPTPASAILSGLLIKTGVYGLWRVFLMVMGPASSQEVELYQQYITNWGMGLFWIGIITMVLGALMALLQTSAKKMLA
YSSVSQVGFIIMGLGAAVYLGTDGPIGFAGGIMHVINHALFKACLFLIVGAIFIRTHLLDIDRVRGMFKQMPFMGIAFII
AALGIAGVPLFNGYASKTILHHALSEVSYLTPGTAMFIADKIFVFTSALTVSYFIKLFRGLFLGKLPQEWQNTNFSVAPI
IKVVVSVFVVLIIAIGVFPNFVLEQFVMPTMAGFPYESEVVTSQLQGMDLFTSKDLSKVVVVFLIAGTITLADQKFHWSR
ITPPYWLSIEALVYRNAITKLMSGIEKLGTTFDVSVQDFYETTGRTSVKVCRKLSELDKTLTDAYDKSGEGAMKLSEKTG
QLDRQLDRFYDSTGEKASSMTVLWPVSKDGDKTLAQKEFEAELEKASSKQGKVSKYVTRDRKARTGFLGKINFDPSKLTV
KNLNFDSFVIAFMLGISLLILIFYHRIF
>Mature_747_residues
TDLVTEQAEFTSILPILIIFVPLLGSFGVILISSVSEKLRDIYTVIISAVPLVLLAVMYPLVQQGIVQYNIEWFLEIGLI
FRLDSLGFLFTALISLIWFLATLYATEYISHEHNRNRFYFFWLFTFGATLGVFVTGDLFGLFVFFEMMSLTSYVLVIHEE
DKDAMRAGNLFLFLGIGGGLAILFGLFIMYFNLETLEIAPMLGDMVAAGVNIPVLLLLFIIGFGIKAGIVPLHIWLPKAH
PVAPTPASAILSGLLIKTGVYGLWRVFLMVMGPASSQEVELYQQYITNWGMGLFWIGIITMVLGALMALLQTSAKKMLAY
SSVSQVGFIIMGLGAAVYLGTDGPIGFAGGIMHVINHALFKACLFLIVGAIFIRTHLLDIDRVRGMFKQMPFMGIAFIIA
ALGIAGVPLFNGYASKTILHHALSEVSYLTPGTAMFIADKIFVFTSALTVSYFIKLFRGLFLGKLPQEWQNTNFSVAPII
KVVVSVFVVLIIAIGVFPNFVLEQFVMPTMAGFPYESEVVTSQLQGMDLFTSKDLSKVVVVFLIAGTITLADQKFHWSRI
TPPYWLSIEALVYRNAITKLMSGIEKLGTTFDVSVQDFYETTGRTSVKVCRKLSELDKTLTDAYDKSGEGAMKLSEKTGQ
LDRQLDRFYDSTGEKASSMTVLWPVSKDGDKTLAQKEFEAELEKASSKQGKVSKYVTRDRKARTGFLGKINFDPSKLTVK
NLNFDSFVIAFMLGISLLILIFYHRIF

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=382, Percent_Identity=27.7486910994764, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI251831116, Length=289, Percent_Identity=25.9515570934256, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1788827, Length=395, Percent_Identity=34.1772151898734, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1788613, Length=431, Percent_Identity=31.7865429234339, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1788831, Length=357, Percent_Identity=31.0924369747899, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI2367154, Length=406, Percent_Identity=28.5714285714286, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788829, Length=410, Percent_Identity=29.0243902439024, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1788614, Length=355, Percent_Identity=29.0140845070423, Blast_Score=109, Evalue=7e-25,
Organism=Escherichia coli, GI145693160, Length=466, Percent_Identity=25.5364806866953, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 83077; Mature: 82945

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLVTEQAEFTSILPILIIFVPLLGSFGVILISSVSEKLRDIYTVIISAVPLVLLAVMY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLVQQGIVQYNIEWFLEIGLIFRLDSLGFLFTALISLIWFLATLYATEYISHEHNRNRFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
FFWLFTFGATLGVFVTGDLFGLFVFFEMMSLTSYVLVIHEEDKDAMRAGNLFLFLGIGGG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHCCEEEEEECCCH
LAILFGLFIMYFNLETLEIAPMLGDMVAAGVNIPVLLLLFIIGFGIKAGIVPLHIWLPKA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHCCCEEEEEEECCC
HPVAPTPASAILSGLLIKTGVYGLWRVFLMVMGPASSQEVELYQQYITNWGMGLFWIGII
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHH
TMVLGALMALLQTSAKKMLAYSSVSQVGFIIMGLGAAVYLGTDGPIGFAGGIMHVINHAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHH
FKACLFLIVGAIFIRTHLLDIDRVRGMFKQMPFMGIAFIIAALGIAGVPLFNGYASKTIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHCCHHHHHHH
HHALSEVSYLTPGTAMFIADKIFVFTSALTVSYFIKLFRGLFLGKLPQEWQNTNFSVAPI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHH
IKVVVSVFVVLIIAIGVFPNFVLEQFVMPTMAGFPYESEVVTSQLQGMDLFTSKDLSKVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHCHHHHHH
VVFLIAGTITLADQKFHWSRITPPYWLSIEALVYRNAITKLMSGIEKLGTTFDVSVQDFY
HHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHH
ETTGRTSVKVCRKLSELDKTLTDAYDKSGEGAMKLSEKTGQLDRQLDRFYDSTGEKASSM
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCE
TVLWPVSKDGDKTLAQKEFEAELEKASSKQGKVSKYVTRDRKARTGFLGKINFDPSKLTV
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHCCCCEEEECCCCCCEEE
KNLNFDSFVIAFMLGISLLILIFYHRIF
ECCCHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TDLVTEQAEFTSILPILIIFVPLLGSFGVILISSVSEKLRDIYTVIISAVPLVLLAVMY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLVQQGIVQYNIEWFLEIGLIFRLDSLGFLFTALISLIWFLATLYATEYISHEHNRNRFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
FFWLFTFGATLGVFVTGDLFGLFVFFEMMSLTSYVLVIHEEDKDAMRAGNLFLFLGIGGG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHCCEEEEEECCCH
LAILFGLFIMYFNLETLEIAPMLGDMVAAGVNIPVLLLLFIIGFGIKAGIVPLHIWLPKA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHCCCEEEEEEECCC
HPVAPTPASAILSGLLIKTGVYGLWRVFLMVMGPASSQEVELYQQYITNWGMGLFWIGII
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHH
TMVLGALMALLQTSAKKMLAYSSVSQVGFIIMGLGAAVYLGTDGPIGFAGGIMHVINHAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHH
FKACLFLIVGAIFIRTHLLDIDRVRGMFKQMPFMGIAFIIAALGIAGVPLFNGYASKTIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHCCHHHHHHH
HHALSEVSYLTPGTAMFIADKIFVFTSALTVSYFIKLFRGLFLGKLPQEWQNTNFSVAPI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHH
IKVVVSVFVVLIIAIGVFPNFVLEQFVMPTMAGFPYESEVVTSQLQGMDLFTSKDLSKVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHCHHHHHH
VVFLIAGTITLADQKFHWSRITPPYWLSIEALVYRNAITKLMSGIEKLGTTFDVSVQDFY
HHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHH
ETTGRTSVKVCRKLSELDKTLTDAYDKSGEGAMKLSEKTGQLDRQLDRFYDSTGEKASSM
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCE
TVLWPVSKDGDKTLAQKEFEAELEKASSKQGKVSKYVTRDRKARTGFLGKINFDPSKLTV
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHCCCCEEEECCCCCCEEE
KNLNFDSFVIAFMLGISLLILIFYHRIF
ECCCHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]