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The map label for this gene is nth [H]

Identifier: 188585131

GI number: 188585131

Start: 520731

End: 521387

Strand: Direct

Name: nth [H]

Synonym: Nther_0492

Alternate gene names: 188585131

Gene position: 520731-521387 (Clockwise)

Preceding gene: 188585130

Following gene: 188585132

Centisome position: 16.45

GC content: 36.38

Gene sequence:

>657_bases
ATGGAGAAAAAAGAGGCTGAACAAATATTATCTAAGTTGAAAGCTAATTACCCTAGTGCGAGAACTGCCTTAAAGTTTAA
CTCACCTTTTGAGTTGTTAATTGCAACAATCTTATCGGCTCAGTGTACTGATATTAGGGTTAATGAAATTACTAAAGAAC
TATTTGAATTAGCTAATACTCCTCAAGATATTTTAAAACTTGGCCGCCCACGACTAATACAAATAATAAAAGGGGCTGGC
TTATATAAAAACAAAAGTAAAAACATCCTGGAAACATGTGAAATTTTAGTAGATGAATATGAAGGAGAAGTTCCTGCTAA
GAGAGAAGAATTGGAAAAACTACCGGGAGTAGGTAGAAAAACGGCTAATGTAGTTTTGGCTAATGCTTTTAACATTCCTG
CCTTTGCGGTAGATACTCATGTTTTACGGGTGTCCAAAAGATTGGGATTGACTGATAAAGAGGACCCCCGCGGAGTGGAA
CAAGACCTTATGTCAGTATTTGATAGAGATGACTGGAATGTAGGACATCATTTATTAATATACCACGGACGAGCAGTATG
TAAGGCCAGGAAGCCTCAATGTGAAAATTGCAGTATTATAGAACATTGTAAGTACTATCATGATAACTATCAATATCCAA
ACAGTACGGGGAGATAA

Upstream 100 bases:

>100_bases
GATGTTCCTCAATGTAGTGACTGCCATGAAGCTATTTGCAGTGAGGCTAAGGAGAGAACATCTTGAAAATAAGGATGATG
TGTTAAAGGATGATGTATTT

Downstream 100 bases:

>100_bases
TATGAACAGCTTAAGCAAACTGCGACAAATGGCTTTAAATAATGGCTTTGTTTCTACTGGAGTCATTTCTCCTGATGAGT
ATAAAGAAGGGAAATCAAGA

Product: DNA-(apurinic or apyrimidinic site) lyase, endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MEKKEAEQILSKLKANYPSARTALKFNSPFELLIATILSAQCTDIRVNEITKELFELANTPQDILKLGRPRLIQIIKGAG
LYKNKSKNILETCEILVDEYEGEVPAKREELEKLPGVGRKTANVVLANAFNIPAFAVDTHVLRVSKRLGLTDKEDPRGVE
QDLMSVFDRDDWNVGHHLLIYHGRAVCKARKPQCENCSIIEHCKYYHDNYQYPNSTGR

Sequences:

>Translated_218_residues
MEKKEAEQILSKLKANYPSARTALKFNSPFELLIATILSAQCTDIRVNEITKELFELANTPQDILKLGRPRLIQIIKGAG
LYKNKSKNILETCEILVDEYEGEVPAKREELEKLPGVGRKTANVVLANAFNIPAFAVDTHVLRVSKRLGLTDKEDPRGVE
QDLMSVFDRDDWNVGHHLLIYHGRAVCKARKPQCENCSIIEHCKYYHDNYQYPNSTGR
>Mature_218_residues
MEKKEAEQILSKLKANYPSARTALKFNSPFELLIATILSAQCTDIRVNEITKELFELANTPQDILKLGRPRLIQIIKGAG
LYKNKSKNILETCEILVDEYEGEVPAKREELEKLPGVGRKTANVVLANAFNIPAFAVDTHVLRVSKRLGLTDKEDPRGVE
QDLMSVFDRDDWNVGHHLLIYHGRAVCKARKPQCENCSIIEHCKYYHDNYQYPNSTGR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=171, Percent_Identity=32.1637426900585, Blast_Score=88, Evalue=7e-18,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=48.2926829268293, Blast_Score=192, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17554540, Length=169, Percent_Identity=33.7278106508876, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=202, Percent_Identity=27.7227722772277, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319304, Length=178, Percent_Identity=29.2134831460674, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550361, Length=181, Percent_Identity=32.5966850828729, Blast_Score=97, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24782; Mature: 24782

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKEAEQILSKLKANYPSARTALKFNSPFELLIATILSAQCTDIRVNEITKELFELANT
CCHHHHHHHHHHHHCCCCCHHEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
PQDILKLGRPRLIQIIKGAGLYKNKSKNILETCEILVDEYEGEVPAKREELEKLPGVGRK
HHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLANAFNIPAFAVDTHVLRVSKRLGLTDKEDPRGVEQDLMSVFDRDDWNVGHHLLI
HHHEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEE
YHGRAVCKARKPQCENCSIIEHCKYYHDNYQYPNSTGR
EECHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEKKEAEQILSKLKANYPSARTALKFNSPFELLIATILSAQCTDIRVNEITKELFELANT
CCHHHHHHHHHHHHCCCCCHHEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
PQDILKLGRPRLIQIIKGAGLYKNKSKNILETCEILVDEYEGEVPAKREELEKLPGVGRK
HHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLANAFNIPAFAVDTHVLRVSKRLGLTDKEDPRGVEQDLMSVFDRDDWNVGHHLLI
HHHEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEE
YHGRAVCKARKPQCENCSIIEHCKYYHDNYQYPNSTGR
EECHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274032; 9823893 [H]