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The map label for this gene is sleB [H]

Identifier: 188585123

GI number: 188585123

Start: 513992

End: 514717

Strand: Reverse

Name: sleB [H]

Synonym: Nther_0484

Alternate gene names: 188585123

Gene position: 514717-513992 (Counterclockwise)

Preceding gene: 188585124

Following gene: 188585107

Centisome position: 16.26

GC content: 41.32

Gene sequence:

>726_bases
ATGTTAACAAAAGGGGGGATCAGATTGTTACGAGTTTCAAAAGTGCTAGTAGTTTGTCTGTTAGTCAGTGGGTTATTATT
CACATCCTTAGGAGGTGGCCAAGTTCAGGCAGCTTCTGAAACCCACACAGTATCTTCCGGGGAAACTCTTTGGAGAATTT
CCCAGTGGTATGATGTATCCTTGGAAGATTTGAGAAGTGTAAACAACATATGGCACAATTATATTTATCCTGGACAAACT
TTAACAATTCCATCAGGTCAGGAAACTGAAACCAAGGAATCCAGTAATGAGATTCAGGAAACTGAAACCGAGGGATCTAA
TAGTGAGAGCCAGGACACTGAAAGTGAATCTAGTTGGCGCAGACTAGATATATCTAGTTATGAACGAGACTTAATCGCCC
GTATAGTTTATAGTGAAGCCAGGGGAGAACCCTATGAAGGGCAAGTAGCTGTTGCCTCAGTAGTTATCAACAGAGTTCTC
GATAATCGATGGCCAAACAATGTAGAAGGGGTAATATTTGAACCATTAGCATTTACACCTGTTCATAATGGCCAATTCTG
GCTAACACCAAATCAAACTGCCTACGATGCTGTAGAAGATGCACTCAAAGGTTGGGATCCCAGTGATGGAGCGACCTTCT
TTTACAATCCCATCACCGCTACATCAAGCTGGATTTTCACTAGAACGACAATTAAACAAATTGGAAGCCATGTATTTGCC
TATTAA

Upstream 100 bases:

>100_bases
AGATGTTTTTTAATCACATTCTGTTTAGATTACTATACAGGAAATTTTACTGATTTGTAAGCTTGTACCCCCCCTGTTAT
AATCGATGGAGTACAATTTT

Downstream 100 bases:

>100_bases
GCATTAACGACTCTCCTTTTGGAGGGTCGTTTTTTAATTAAAGGTCTATAGCAAGGTGGTTAAGATGTTTTTTTCGCATA
CCAAATTACAAGTTAATCGA

Product: cell wall hydrolase SleB

Products: NA

Alternate protein names: SCLE [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MLTKGGIRLLRVSKVLVVCLLVSGLLFTSLGGGQVQAASETHTVSSGETLWRISQWYDVSLEDLRSVNNIWHNYIYPGQT
LTIPSGQETETKESSNEIQETETEGSNSESQDTESESSWRRLDISSYERDLIARIVYSEARGEPYEGQVAVASVVINRVL
DNRWPNNVEGVIFEPLAFTPVHNGQFWLTPNQTAYDAVEDALKGWDPSDGATFFYNPITATSSWIFTRTTIKQIGSHVFA
Y

Sequences:

>Translated_241_residues
MLTKGGIRLLRVSKVLVVCLLVSGLLFTSLGGGQVQAASETHTVSSGETLWRISQWYDVSLEDLRSVNNIWHNYIYPGQT
LTIPSGQETETKESSNEIQETETEGSNSESQDTESESSWRRLDISSYERDLIARIVYSEARGEPYEGQVAVASVVINRVL
DNRWPNNVEGVIFEPLAFTPVHNGQFWLTPNQTAYDAVEDALKGWDPSDGATFFYNPITATSSWIFTRTTIKQIGSHVFA
Y
>Mature_241_residues
MLTKGGIRLLRVSKVLVVCLLVSGLLFTSLGGGQVQAASETHTVSSGETLWRISQWYDVSLEDLRSVNNIWHNYIYPGQT
LTIPSGQETETKESSNEIQETETEGSNSESQDTESESSWRRLDISSYERDLIARIVYSEARGEPYEGQVAVASVVINRVL
DNRWPNNVEGVIFEPLAFTPVHNGQFWLTPNQTAYDAVEDALKGWDPSDGATFFYNPITATSSWIFTRTTIKQIGSHVFA
Y

Specific function: Could be a lytic transglycosylase. Required for spore cortex hydrolysis during germination. Interacts strongly but noncovalently with spore components [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 26949; Mature: 26949

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKGGIRLLRVSKVLVVCLLVSGLLFTSLGGGQVQAASETHTVSSGETLWRISQWYDVS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEHHHCCCC
LEDLRSVNNIWHNYIYPGQTLTIPSGQETETKESSNEIQETETEGSNSESQDTESESSWR
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
RLDISSYERDLIARIVYSEARGEPYEGQVAVASVVINRVLDNRWPNNVEGVIFEPLAFTP
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECE
VHNGQFWLTPNQTAYDAVEDALKGWDPSDGATFFYNPITATSSWIFTRTTIKQIGSHVFA
ECCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHHCC
Y
C
>Mature Secondary Structure
MLTKGGIRLLRVSKVLVVCLLVSGLLFTSLGGGQVQAASETHTVSSGETLWRISQWYDVS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEHHHCCCC
LEDLRSVNNIWHNYIYPGQTLTIPSGQETETKESSNEIQETETEGSNSESQDTESESSWR
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
RLDISSYERDLIARIVYSEARGEPYEGQVAVASVVINRVLDNRWPNNVEGVIFEPLAFTP
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECE
VHNGQFWLTPNQTAYDAVEDALKGWDPSDGATFFYNPITATSSWIFTRTTIKQIGSHVFA
ECCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHHCC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8830707; 8760912; 9384377; 10197998; 10658652 [H]