Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 188585094

GI number: 188585094

Start: 479799

End: 481091

Strand: Direct

Name: purB [H]

Synonym: Nther_0455

Alternate gene names: 188585094

Gene position: 479799-481091 (Clockwise)

Preceding gene: 188585093

Following gene: 188585095

Centisome position: 15.16

GC content: 37.82

Gene sequence:

>1293_bases
ATGATAGAGCGTTATCAAAGGCCGGAAATGAAGCAGTTATGGAGCTTGGAAAATAAATTTAAGAAATGGTTGCAGGTGGA
GATCTATTCATGTGAAGCCTTTTCTGAACTAGGAGAAGTCCCTGGGGAAGCAGTAGATAAAATTAAATCCAATGCCTACT
TTGATACACAAAGAGTACTTGAATTGGAAAAGGAGACGCGCCATGATGTGATTGCCTTTACGCGCGCAGTATCTGAAACT
TTAGGACCAGAAAAAAAATATGTTCATTACGGTCTTACATCTTCAGATGTTGTTGATACAGCACTATCATCTTTGATTAG
AGATGCTTTGACATTGATAGAGCAAGAACTTGACAGGGTGCTCCAGATTTTGCGAGACCAGGCCCGGAAATACAAAGATT
ATCCTATTATAGGAAGAACCCACGGTGTCCATGCTGAACCTACAACCTTGGGTCTGAAATTTGCTTTGTTCTACGATGAA
TTGTCACGATCTAGAAAAAGACTAGATAGGGCTAAAGAAAATATCAGTGTTGGTAAAATTTCGGGAGCTGTGGGAACTTA
TGCTAATGTCTCTCCCTATGTCGAGGAATATGTGTGCCATAAATTAGGCTTACAACCAGCTTTAACTTCTACACAAATTT
TGCAAAGGGACAGACATGCAGAATTAATGTCAACTCTCGCAATTCTTGCGGGAACTATGGATAAAATAGCAACTGAAATT
AGAAATCTACAGAAAACGGAAGTTCGAGAAGTTGAAGAACCTTTTTATAAAGGTCAAAAGGGATCATCCGCCATGCCCCA
CAAGAGAAATCCTGTTAATTGTGAAAAAATTTCAGGTCTAAGTAGGGTTATCAGGAGTAATGCCCAGGCAGCCTTTGAAA
ATCAACCTCTATGGCATGAACGAGATATTTCACACTCATCTGTGGAAAGGTTAATTATTCCCGATAGTACTACTTTATCT
CATTATTTACTCAATCTTTTGGCTAAAGTATTGGAAAACTTGCATGTGTATCCAGACAACATGGAACAAAATTTAAAGAC
CACTAATGGATTGATATACAGTCAAAGAGTGTTACTTAACTTGATTGATAAAGGTTTATCTCGAGAGAAGGCCTATGATT
TAATTCAAGAATTGGCAATGAAGTCGTGGGAAGGTGACGAAAATTTCCAAGAGCTGTTAAAACAAGATTCCAGGGTATTG
GAGATTCTCGATAAAGAAGAAATAGATAATTGTTTCGACCCTACTTATTATACCAGAAACGTGGATTATATTTTTGAGAG
ATTAGGTATCTAA

Upstream 100 bases:

>100_bases
ATGATTATCAAGAACAAAATCGTCAAAAGGTTTTAGAGAAGGGGCAAGAGTTACAAAAAATAGGTCACGAACAATATTTG
AAAAGAAAGGAAGAAAGCTG

Downstream 100 bases:

>100_bases
TTTATATTTGAGGATTAAGAAGGAGGGGACTTCTTTGACTGGAAATAGTTATCGTAAAGCTGGTGTGGATGTGGACAAGG
GAAATAAAATAGCGGAAAAC

Product: Adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MIERYQRPEMKQLWSLENKFKKWLQVEIYSCEAFSELGEVPGEAVDKIKSNAYFDTQRVLELEKETRHDVIAFTRAVSET
LGPEKKYVHYGLTSSDVVDTALSSLIRDALTLIEQELDRVLQILRDQARKYKDYPIIGRTHGVHAEPTTLGLKFALFYDE
LSRSRKRLDRAKENISVGKISGAVGTYANVSPYVEEYVCHKLGLQPALTSTQILQRDRHAELMSTLAILAGTMDKIATEI
RNLQKTEVREVEEPFYKGQKGSSAMPHKRNPVNCEKISGLSRVIRSNAQAAFENQPLWHERDISHSSVERLIIPDSTTLS
HYLLNLLAKVLENLHVYPDNMEQNLKTTNGLIYSQRVLLNLIDKGLSREKAYDLIQELAMKSWEGDENFQELLKQDSRVL
EILDKEEIDNCFDPTYYTRNVDYIFERLGI

Sequences:

>Translated_430_residues
MIERYQRPEMKQLWSLENKFKKWLQVEIYSCEAFSELGEVPGEAVDKIKSNAYFDTQRVLELEKETRHDVIAFTRAVSET
LGPEKKYVHYGLTSSDVVDTALSSLIRDALTLIEQELDRVLQILRDQARKYKDYPIIGRTHGVHAEPTTLGLKFALFYDE
LSRSRKRLDRAKENISVGKISGAVGTYANVSPYVEEYVCHKLGLQPALTSTQILQRDRHAELMSTLAILAGTMDKIATEI
RNLQKTEVREVEEPFYKGQKGSSAMPHKRNPVNCEKISGLSRVIRSNAQAAFENQPLWHERDISHSSVERLIIPDSTTLS
HYLLNLLAKVLENLHVYPDNMEQNLKTTNGLIYSQRVLLNLIDKGLSREKAYDLIQELAMKSWEGDENFQELLKQDSRVL
EILDKEEIDNCFDPTYYTRNVDYIFERLGI
>Mature_430_residues
MIERYQRPEMKQLWSLENKFKKWLQVEIYSCEAFSELGEVPGEAVDKIKSNAYFDTQRVLELEKETRHDVIAFTRAVSET
LGPEKKYVHYGLTSSDVVDTALSSLIRDALTLIEQELDRVLQILRDQARKYKDYPIIGRTHGVHAEPTTLGLKFALFYDE
LSRSRKRLDRAKENISVGKISGAVGTYANVSPYVEEYVCHKLGLQPALTSTQILQRDRHAELMSTLAILAGTMDKIATEI
RNLQKTEVREVEEPFYKGQKGSSAMPHKRNPVNCEKISGLSRVIRSNAQAAFENQPLWHERDISHSSVERLIIPDSTTLS
HYLLNLLAKVLENLHVYPDNMEQNLKTTNGLIYSQRVLLNLIDKGLSREKAYDLIQELAMKSWEGDENFQELLKQDSRVL
EILDKEEIDNCFDPTYYTRNVDYIFERLGI

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=438, Percent_Identity=28.9954337899543, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=30.0261096605744, Blast_Score=168, Evalue=9e-42,
Organism=Escherichia coli, GI1787376, Length=314, Percent_Identity=28.0254777070064, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787896, Length=208, Percent_Identity=28.8461538461538, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=382, Percent_Identity=30.3664921465969, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32564234, Length=327, Percent_Identity=32.1100917431193, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323391, Length=437, Percent_Identity=30.6636155606407, Blast_Score=183, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24647570, Length=444, Percent_Identity=27.4774774774775, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24640177, Length=218, Percent_Identity=29.8165137614679, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640179, Length=218, Percent_Identity=29.8165137614679, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49519; Mature: 49519

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYQRPEMKQLWSLENKFKKWLQVEIYSCEAFSELGEVPGEAVDKIKSNAYFDTQRVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHH
ELEKETRHDVIAFTRAVSETLGPEKKYVHYGLTSSDVVDTALSSLIRDALTLIEQELDRV
HHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQILRDQARKYKDYPIIGRTHGVHAEPTTLGLKFALFYDELSRSRKRLDRAKENISVGKI
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEE
SGAVGTYANVSPYVEEYVCHKLGLQPALTSTQILQRDRHAELMSTLAILAGTMDKIATEI
CCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQKTEVREVEEPFYKGQKGSSAMPHKRNPVNCEKISGLSRVIRSNAQAAFENQPLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCC
RDISHSSVERLIIPDSTTLSHYLLNLLAKVLENLHVYPDNMEQNLKTTNGLIYSQRVLLN
CCCCHHHHCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHH
LIDKGLSREKAYDLIQELAMKSWEGDENFQELLKQDSRVLEILDKEEIDNCFDPTYYTRN
HHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VDYIFERLGI
HHHHHHHHCC
>Mature Secondary Structure
MIERYQRPEMKQLWSLENKFKKWLQVEIYSCEAFSELGEVPGEAVDKIKSNAYFDTQRVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHH
ELEKETRHDVIAFTRAVSETLGPEKKYVHYGLTSSDVVDTALSSLIRDALTLIEQELDRV
HHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQILRDQARKYKDYPIIGRTHGVHAEPTTLGLKFALFYDELSRSRKRLDRAKENISVGKI
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEE
SGAVGTYANVSPYVEEYVCHKLGLQPALTSTQILQRDRHAELMSTLAILAGTMDKIATEI
CCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQKTEVREVEEPFYKGQKGSSAMPHKRNPVNCEKISGLSRVIRSNAQAAFENQPLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCC
RDISHSSVERLIIPDSTTLSHYLLNLLAKVLENLHVYPDNMEQNLKTTNGLIYSQRVLLN
CCCCHHHHCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHH
LIDKGLSREKAYDLIQELAMKSWEGDENFQELLKQDSRVLEILDKEEIDNCFDPTYYTRN
HHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VDYIFERLGI
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]