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The map label for this gene is dagK [H]

Identifier: 188585057

GI number: 188585057

Start: 435909

End: 436865

Strand: Reverse

Name: dagK [H]

Synonym: Nther_0418

Alternate gene names: 188585057

Gene position: 436865-435909 (Counterclockwise)

Preceding gene: 188585066

Following gene: 188585054

Centisome position: 13.8

GC content: 33.12

Gene sequence:

>957_bases
ATGGTAAAAATAACTAGTGAGGGGTATAATAATAATATGTGTGAAAAAGAAATAGGTGTTATCATCAACCCAACAGCTGG
TAAAGGTAAGGCAAAACAAGTTTGGAACCAAATTTTACCTTTTTTGAAAACAAAAAAAATTAATCTTCGTTATAGATTAA
CAAGTAGTCCCAAAGAAGCAGGTAAGCTAGCAAGTGAGCTTATTCAGGAAGGTTGTTCTAAAATTGCAATTATAGGCGGT
GATGGGACTCTACATGAAGCCATTAACGGCCAAAATATAATTAATGATCGAGTGGCCTTTGGAATAATACCTGCAGGAAC
AGGTAATGACTTGGTGCGAACATTAAATATTCCTAATGATCCACTACAAGCCTGTCAGGTTATTCTAGATGGATATTACC
AAAAAATTGACCTGGGATTGATTAATGGTGAAACATATTTTGTTAATACCGCTGGAGTAGGCTTTGATGTTGAAATTGCT
AAGCTCATGAATTCTAATAAACGATTATTTTTCAATGGTAAAGGAAGTTATTTCATTTCCATATTAAGAACTTTAATTAC
ATATAGCAATCTCAATTTAATTTTAGAGACCGATGATAAATCTACTGAGATCAGTAATTGTTTTTTACTTTCCATAGGTA
ATGCAAAATACATCGGTGGAGGCATCAAACTTATACCCTCAGCAAAGCCTAATGACGGCTATTTTCATCTTTTAATCGCC
AAGGATATAAAAAGAACCACAGTTGTACGTAAATTTGCTTCGATCTATAAAGGGAATCATGTTGATAACTATCAAGTCAT
GGAAATTAAAACAAAAACAATTAAAATCGAACCCGGTACAAAGAATACCGGGTTGTCAATCAATTATCACTCAGATGGAG
AAATATATGGAAGTATCCCTGCCCATATTCAATTAATTCCTCAAAAACTTCCAATAATAACACCAAAGAATAATTGA

Upstream 100 bases:

>100_bases
TCACAGAAACCAATATTATCCCTCCAATCTATGTTTACTTTTTGTATTTATTTAATAACAGTACTATTTCCAAAACCGTT
CTCCTTTTGTATAATTAAAA

Downstream 100 bases:

>100_bases
TTAATTTATTTCATAACTATTTGGGCAAATTTTTTAAAGGATCTATCATAAGTTTTTTCAACATCCTCAGGGAAACAACA
AGCAGGAAGTTGTTCCATTT

Product: diacylglycerol kinase catalytic region

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MVKITSEGYNNNMCEKEIGVIINPTAGKGKAKQVWNQILPFLKTKKINLRYRLTSSPKEAGKLASELIQEGCSKIAIIGG
DGTLHEAINGQNIINDRVAFGIIPAGTGNDLVRTLNIPNDPLQACQVILDGYYQKIDLGLINGETYFVNTAGVGFDVEIA
KLMNSNKRLFFNGKGSYFISILRTLITYSNLNLILETDDKSTEISNCFLLSIGNAKYIGGGIKLIPSAKPNDGYFHLLIA
KDIKRTTVVRKFASIYKGNHVDNYQVMEIKTKTIKIEPGTKNTGLSINYHSDGEIYGSIPAHIQLIPQKLPIITPKNN

Sequences:

>Translated_318_residues
MVKITSEGYNNNMCEKEIGVIINPTAGKGKAKQVWNQILPFLKTKKINLRYRLTSSPKEAGKLASELIQEGCSKIAIIGG
DGTLHEAINGQNIINDRVAFGIIPAGTGNDLVRTLNIPNDPLQACQVILDGYYQKIDLGLINGETYFVNTAGVGFDVEIA
KLMNSNKRLFFNGKGSYFISILRTLITYSNLNLILETDDKSTEISNCFLLSIGNAKYIGGGIKLIPSAKPNDGYFHLLIA
KDIKRTTVVRKFASIYKGNHVDNYQVMEIKTKTIKIEPGTKNTGLSINYHSDGEIYGSIPAHIQLIPQKLPIITPKNN
>Mature_318_residues
MVKITSEGYNNNMCEKEIGVIINPTAGKGKAKQVWNQILPFLKTKKINLRYRLTSSPKEAGKLASELIQEGCSKIAIIGG
DGTLHEAINGQNIINDRVAFGIIPAGTGNDLVRTLNIPNDPLQACQVILDGYYQKIDLGLINGETYFVNTAGVGFDVEIA
KLMNSNKRLFFNGKGSYFISILRTLITYSNLNLILETDDKSTEISNCFLLSIGNAKYIGGGIKLIPSAKPNDGYFHLLIA
KDIKRTTVVRKFASIYKGNHVDNYQVMEIKTKTIKIEPGTKNTGLSINYHSDGEIYGSIPAHIQLIPQKLPIITPKNN

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derive

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Secreted, cell wall (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=279, Percent_Identity=25.4480286738351, Blast_Score=81, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323289, Length=160, Percent_Identity=30, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 34991; Mature: 34991

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKITSEGYNNNMCEKEIGVIINPTAGKGKAKQVWNQILPFLKTKKINLRYRLTSSPKEA
CEEEECCCCCCCCCCHHCCEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCHHHH
GKLASELIQEGCSKIAIIGGDGTLHEAINGQNIINDRVAFGIIPAGTGNDLVRTLNIPND
HHHHHHHHHCCCCEEEEECCCCCCHHHCCCCCEECCCEEEEEEECCCCCCEEEEECCCCC
PLQACQVILDGYYQKIDLGLINGETYFVNTAGVGFDVEIAKLMNSNKRLFFNGKGSYFIS
HHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCCCCCHHHHHHCCCCEEEECCCCHHHHH
ILRTLITYSNLNLILETDDKSTEISNCFLLSIGNAKYIGGGIKLIPSAKPNDGYFHLLIA
HHHHHHHHCCCEEEEECCCCCCCCCEEEEEEECCCEEECCCEEEECCCCCCCCEEEEEEE
KDIKRTTVVRKFASIYKGNHVDNYQVMEIKTKTIKIEPGTKNTGLSINYHSDGEIYGSIP
CCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCCCCCEEEEECCCCEEEECCC
AHIQLIPQKLPIITPKNN
CEEEEECCCCCEEECCCC
>Mature Secondary Structure
MVKITSEGYNNNMCEKEIGVIINPTAGKGKAKQVWNQILPFLKTKKINLRYRLTSSPKEA
CEEEECCCCCCCCCCHHCCEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCHHHH
GKLASELIQEGCSKIAIIGGDGTLHEAINGQNIINDRVAFGIIPAGTGNDLVRTLNIPND
HHHHHHHHHCCCCEEEEECCCCCCHHHCCCCCEECCCEEEEEEECCCCCCEEEEECCCCC
PLQACQVILDGYYQKIDLGLINGETYFVNTAGVGFDVEIAKLMNSNKRLFFNGKGSYFIS
HHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCCCCCHHHHHHCCCCEEEECCCCHHHHH
ILRTLITYSNLNLILETDDKSTEISNCFLLSIGNAKYIGGGIKLIPSAKPNDGYFHLLIA
HHHHHHHHCCCEEEEECCCCCCCCCEEEEEEECCCEEECCCEEEECCCCCCCCEEEEEEE
KDIKRTTVVRKFASIYKGNHVDNYQVMEIKTKTIKIEPGTKNTGLSINYHSDGEIYGSIP
CCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCCCCCEEEEECCCCEEEECCC
AHIQLIPQKLPIITPKNN
CEEEEECCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]