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The map label for this gene is ndoA [H]

Identifier: 188584950

GI number: 188584950

Start: 326254

End: 326604

Strand: Direct

Name: ndoA [H]

Synonym: Nther_0309

Alternate gene names: 188584950

Gene position: 326254-326604 (Clockwise)

Preceding gene: 188584949

Following gene: 188584951

Centisome position: 10.31

GC content: 33.9

Gene sequence:

>351_bases
GTGAATATAAAACGTGGAGATATATATTATGCAGACCTAAGCCCTGTGATCGGTTCAGAACAGGGTGGAGTCAGACCAGT
TTTAGTAGTACAAAATGATATCGGTAACAAGTACAGCCCCACTGTAATCGTAGTAGCAATCACTTCTCAAATTCAAAAAG
CTAAACTTCCGACCCATGTAGAAATAACCGCTGAAGATTATAATTTAGATAAAAATTCAGTTGTATTGACTGAACAACTT
CGCACAATCGATAAACAGCGGTTAAAAGAAAAAATCACCCATATTGATAAAGAATTAATGAATAAAATAAGTGAAGCAAT
TAAGATCAGTCTTGGATTAATAGAATTCTAA

Upstream 100 bases:

>100_bases
AGATGGCCTCAATCAATTTGGAGATAGCTAATGAAGCACTGGAGGCTGAGAATGAAGCTAATCAATTAGCAGAAGATATA
GTTAGTGGAGTGTGAGAAAA

Downstream 100 bases:

>100_bases
AACGTAGTGTAATCACTGCGTTTTTTATATTTTAAAGTGGTAGAGGAGGTTTTTTATGGAGGAGTTTAAGAAAGCGTTAA
CAAAGAGAGTATCATTAGTA

Product: transcriptional modulator of MazE/toxin, MazF

Products: NA

Alternate protein names: Endoribonuclease EndoA; Toxin EndoA [H]

Number of amino acids: Translated: 116; Mature: 116

Protein sequence:

>116_residues
MNIKRGDIYYADLSPVIGSEQGGVRPVLVVQNDIGNKYSPTVIVVAITSQIQKAKLPTHVEITAEDYNLDKNSVVLTEQL
RTIDKQRLKEKITHIDKELMNKISEAIKISLGLIEF

Sequences:

>Translated_116_residues
MNIKRGDIYYADLSPVIGSEQGGVRPVLVVQNDIGNKYSPTVIVVAITSQIQKAKLPTHVEITAEDYNLDKNSVVLTEQL
RTIDKQRLKEKITHIDKELMNKISEAIKISLGLIEF
>Mature_116_residues
MNIKRGDIYYADLSPVIGSEQGGVRPVLVVQNDIGNKYSPTVIVVAITSQIQKAKLPTHVEITAEDYNLDKNSVVLTEQL
RTIDKQRLKEKITHIDKELMNKISEAIKISLGLIEF

Specific function: Part of a toxin-antitoxin (TA) module. Has specificity for UAC sequences, yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 13004; Mature: 13004

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKRGDIYYADLSPVIGSEQGGVRPVLVVQNDIGNKYSPTVIVVAITSQIQKAKLPTHV
CCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCHHHHCCCCCEE
EITAEDYNLDKNSVVLTEQLRTIDKQRLKEKITHIDKELMNKISEAIKISLGLIEF
EEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECC
>Mature Secondary Structure
MNIKRGDIYYADLSPVIGSEQGGVRPVLVVQNDIGNKYSPTVIVVAITSQIQKAKLPTHV
CCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCHHHHCCCCCEE
EITAEDYNLDKNSVVLTEQLRTIDKQRLKEKITHIDKELMNKISEAIKISLGLIEF
EEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]